EP2834649A4 - Zellkultur sowie gradientenmigrations-testverfahren und -vorrichtungen - Google Patents

Zellkultur sowie gradientenmigrations-testverfahren und -vorrichtungen

Info

Publication number
EP2834649A4
EP2834649A4 EP13772405.0A EP13772405A EP2834649A4 EP 2834649 A4 EP2834649 A4 EP 2834649A4 EP 13772405 A EP13772405 A EP 13772405A EP 2834649 A4 EP2834649 A4 EP 2834649A4
Authority
EP
European Patent Office
Prior art keywords
devices
cell culture
assay methods
migration assay
gradient migration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13772405.0A
Other languages
English (en)
French (fr)
Other versions
EP2834649A1 (de
Inventor
Ju-Sung Paul Hung
Philip J Lee
Amedeo J Cappione
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
EMD Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/436,992 external-priority patent/US9637715B2/en
Application filed by EMD Millipore Corp filed Critical EMD Millipore Corp
Publication of EP2834649A1 publication Critical patent/EP2834649A1/de
Publication of EP2834649A4 publication Critical patent/EP2834649A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Urology & Nephrology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP13772405.0A 2012-04-01 2013-02-06 Zellkultur sowie gradientenmigrations-testverfahren und -vorrichtungen Withdrawn EP2834649A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/436,992 US9637715B2 (en) 2005-07-07 2012-04-01 Cell culture and invasion assay method and system
US201361761227P 2013-02-05 2013-02-05
PCT/US2013/024999 WO2013151616A1 (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Publications (2)

Publication Number Publication Date
EP2834649A1 EP2834649A1 (de) 2015-02-11
EP2834649A4 true EP2834649A4 (de) 2015-12-09

Family

ID=49300898

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13772405.0A Withdrawn EP2834649A4 (de) 2012-04-01 2013-02-06 Zellkultur sowie gradientenmigrations-testverfahren und -vorrichtungen

Country Status (5)

Country Link
EP (1) EP2834649A4 (de)
JP (2) JP2015517804A (de)
CN (2) CN106399094A (de)
SG (3) SG11201405210QA (de)
WO (1) WO2013151616A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2992206B1 (fr) * 2012-06-21 2014-07-18 Oreal Composition cosmetique comprenant une huile, des particules d'aerogel de silice hydrophobe et une resine hydrocarbonee
CA2901950A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
US10421949B2 (en) 2013-02-22 2019-09-24 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
EP3112450A4 (de) * 2014-02-25 2017-11-08 Kyoto University Mikrofluidische vorrichtung und dreidimensionales mikrokulturverfahren für zellen
TWI743024B (zh) * 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
RU2712746C2 (ru) 2014-12-22 2020-01-30 Джензим Корпорейшн Способы культивирования клетки млекопитающего
CN105062879B (zh) * 2015-07-26 2017-06-27 江苏大学附属医院 细胞多向迁移实验用装置
CN105062890A (zh) * 2015-09-11 2015-11-18 温州梅塔光学科技有限公司 一种单细胞实验装置
NL2016404B1 (en) 2016-03-09 2017-09-26 Mimetas B V Double tubular structures.
RU2741806C2 (ru) 2016-06-15 2021-01-28 Миметас Б.В. Устройство и способы для культивирования клеток
EP3478409A1 (de) * 2016-06-29 2019-05-08 Miltenyi Biotec GmbH Mehrstufige einwegkassette für biologische proben
WO2018234163A1 (en) * 2017-06-16 2018-12-27 İni̇ti̇o Biyomedikal Mühendislik Danişmanlik Sanayi Ve Ticaret Limited Şirketi METHOD FOR DETERMINING CELL MIGRATION AND INVASION
JP6812944B2 (ja) * 2017-10-18 2021-01-13 株式会社島津製作所 質量分析装置用情報管理装置
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
CN108641931A (zh) * 2018-04-04 2018-10-12 浙江大学 一种数字化微阵列器官芯片及其应用
CN111057639B (zh) * 2019-11-29 2022-06-17 中国医科大学 一种基于微流控芯片的循环肿瘤细胞分离富集系统和方法
EP4177350A1 (de) 2020-07-06 2023-05-10 Nikon Corporation Vorrichtung zur berechnung der migrationsfähigkeit, verfahren zur beurteilung der migrationsfähigkeit und computerprogramm zur veranlassung eines computers zur ausführung des migrationsfähigkeitsbeurteilungsverfahrens
WO2022018743A1 (en) * 2020-07-22 2022-01-27 INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) A system and method for simultaneous live-cell imaging and growing
CN112646713A (zh) * 2020-12-25 2021-04-13 中国科学院广州生物医药与健康研究院 一种整合性肿瘤细胞行为实验用芯片
NL2028424B1 (en) 2021-06-10 2022-12-20 Mimetas B V Method and apparatus for forming a microfluidic gel structure
CN113648287B (zh) * 2021-07-30 2023-05-05 深圳华源再生医学有限公司 细胞装载装置及其制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012024646A2 (en) * 2010-08-19 2012-02-23 The Johns Hopkins University Cell culture system and method of use thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1546299A4 (de) * 2002-08-27 2007-07-04 Univ Vanderbilt Kapillarperfundierte bioreaktoren mit mehreren kammern
JP2007515958A (ja) * 2003-12-19 2007-06-21 ユニヴァーシティー オブ ウォータールー 培養細胞、細胞培養の方法および機器
US20080233607A1 (en) * 2004-11-11 2008-09-25 Hanry Yu Cell Culture Device
US9388374B2 (en) * 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
WO2009062095A1 (en) * 2007-11-07 2009-05-14 Bellbrook Labs, Llc Microfluidic device having stable static gradient for analyzing chemotaxis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012024646A2 (en) * 2010-08-19 2012-02-23 The Johns Hopkins University Cell culture system and method of use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHENG SHING-YI ET AL: "A hydrogel-based microfluidic device for the studies of directed cell migration", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY - CAMBRIDGE, GB, vol. 7, no. 6, 1 June 2007 (2007-06-01), pages 763 - 769, XP002497558, ISSN: 1473-0197, [retrieved on 20070404], DOI: 10.1039/B618463D *
See also references of WO2013151616A1 *
SEOK CHUNG ET AL: "Cell migration into scaffolds under co-culture conditions in a microfluidic platform", LAB ON A CHIP, vol. 9, no. 2, 1 January 2009 (2009-01-01), pages 269, XP055066343, ISSN: 1473-0197, DOI: 10.1039/b807585a *

Also Published As

Publication number Publication date
WO2013151616A1 (en) 2013-10-10
SG10201803961VA (en) 2018-07-30
CN104412109A (zh) 2015-03-11
SG11201405210QA (en) 2014-09-26
EP2834649A1 (de) 2015-02-11
SG10201607963SA (en) 2016-11-29
JP2017093467A (ja) 2017-06-01
CN106399094A (zh) 2017-02-15
JP2015517804A (ja) 2015-06-25

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