EP2656916A1 - Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides - Google Patents

Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides Download PDF

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Publication number
EP2656916A1
EP2656916A1 EP12002849.3A EP12002849A EP2656916A1 EP 2656916 A1 EP2656916 A1 EP 2656916A1 EP 12002849 A EP12002849 A EP 12002849A EP 2656916 A1 EP2656916 A1 EP 2656916A1
Authority
EP
European Patent Office
Prior art keywords
syringe
dosing
pipette
metering
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP12002849.3A
Other languages
German (de)
English (en)
Other versions
EP2656916B1 (fr
Inventor
Herbert Belgardt
Burkhardt Reichmuth
Peter Molitor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP12002849.3A priority Critical patent/EP2656916B1/fr
Priority to PL12002849T priority patent/PL2656916T3/pl
Publication of EP2656916A1 publication Critical patent/EP2656916A1/fr
Application granted granted Critical
Publication of EP2656916B1 publication Critical patent/EP2656916B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information

Definitions

  • the invention relates to a method for dosing liquid by means of a pipette and a syringe and to a pipette for actuating a syringe for dosing liquid.
  • the pipettes used here for actuating a syringe serve to dispense liquid received in the syringe in several steps. They are also referred to as dispensers or repeating pipettes. These pipettes have at the lower end of a rod-shaped housing a receptacle for a flange of a cylinder of the syringe and in the housing a displaceable receiving body with a piston receptacle for the upper end portion of a piston rod of a piston of the syringe.
  • the syringe can be used with the flange and the end portion of the piston rod through axially directed openings of the receptacles.
  • the flange and the end portion are held in the receptacles by means for detachable holding, which are designed, for example, as spring-loaded gripping levers.
  • the pipette comprises means for displacing the receiving body, which allow a partial withdrawal of the piston from the cylinder for the suction of liquid into the syringe and a stepwise depression of the piston into the cylinder for the stepwise discharge of liquid.
  • the DE 2926691 C2 and the US 4406170 A describe means for displacing the receiving body in the housing. These include a connected to the receiving body and by a straight line slot protruding from the housing elevator lever for drawing liquid into the syringe by displacing the receiving body of the recording away. In addition, they include a rack-pawl means for gradually advancing the piston by a reciprocating metering lever. At the metering lever is a pivoting pawl stored. The rack is connected to the receiving body and arranged in the pivoting range of the pawl. An adjustable sliding cover covers more or less the row of teeth on the rack in order to limit the engagement of the pawl in the rack when pivoting the metering lever.
  • the rack is designed with a contour through which in the advanced position of the piston, the cover of the rack is displaced away, so that it prevents the pawl from engaging in the uncovered teeth of the rack. This prevents that a residual amount is discharged from the syringe, which is smaller than the dispensed in each dosing step dosage.
  • the EP 0657216 B1 and US 5620661 A describe such a pipette with a sensor for sensing protrusions and depressions on the syringe flange and associated syringes.
  • the sensor is used to determine the size of the syringe used.
  • electronics determines the amount of fluid dispensed at each dispensing step. This is displayed on a display.
  • the known manually operated repeating pipettes have the disadvantage that the user has to look up the maximum number of dosing steps that are possible with completely filled syringe with the set dosing volume after performing a reverse stroke without refilling the syringe in the manual or experimentally determined. Furthermore, the user must count the dosing steps performed if he wants to know the number of additional dosing steps that are still possible with the remaining liquid in the syringe. This is particularly troublesome and error prone with small dosing increments.
  • the object of the invention is to provide a method for dosing liquid by means of a pipette and a syringe and a pipette for actuating a syringe for dosing liquid with more favorable application properties.
  • the user is shown the maximum number of metering steps which, when the syringe is completely filled, are displayed at the setpoint Dosing volume after performing a reverse stroke without refilling the syringe are possible. Furthermore, the user is shown the number of dosing steps performed and / or the number of dosing steps still possible without refilling the syringe with the set dosing increment.
  • a residual amount of liquid remains that is less than the set metering volume.
  • the user After performing the maximum number of dosing steps, the user either has the option of dislodging the used syringe from the pipette and, after inserting a new syringe, performing the procedure again. Alternatively, the user may use the same syringe multiple times. If he repeatedly uses the syringe, the display device displays the total number of dosing steps performed by the same syringe. The user can thus carry out the dosing processes more easily and with a reduced risk of error.
  • the number of executed dosing steps is displayed instead of the display of the still possible number of dosing steps.
  • the number of refilling of the syringe still possible dosing steps is displayed.
  • counting up is performed, and counting down is performed in the second-mentioned embodiment.
  • the number of possible dosing steps is the difference between the possible maximum number of dosing steps and the number of dosing steps performed.
  • the number of metering steps performed and the number of possible metering steps until the syringe is completely emptied are displayed.
  • one of the two abovementioned numbers is displayed larger than the other one. For example, during dispensing the sum of the executed number of dosing steps greater than the number of possible dosing steps displayed.
  • the method is carried out with a hand-driven pipette.
  • a hand-driven pipette both the drawing-in of liquid into the syringe and the gradual discharge of liquid through the syringe are performed by means of a mechanical drive which the user drives by muscle power.
  • the pipette detects a coding of the syringe connected to the pipette and indicates the metering volume on the basis of the set metering increment and the detected coding and displays it by means of the display device.
  • This embodiment allows the use of different sized syringes, which always automatically the dosing volume is displayed, which is delivered at the set dosing increment with each syringe used.
  • a display indicative of the picking up of liquid is indicated by means of the display device.
  • the display is according to an embodiment an upward arrow or other obvious symbol. This facilitates the operation of the pipette.
  • a flashing or otherwise indicative of the current process of receiving liquid into the syringe and performing a reverse stroke is displayed.
  • the user is instructed to fill the syringe completely with liquid as far as possible and to perform the reverse stroke.
  • operator errors and dosing errors can be avoided.
  • the set metering volume and the number of metering steps have been indicated.
  • the user can obtain information at any time about the set dosing volume and the number of doses taken.
  • a display indicative of the dispensing of liquid is displayed.
  • the display is according to one embodiment, a downward arrow or other obvious symbol. This facilitates the operation of the pipette.
  • a display indicating the optional dispensing of residual liquid from the syringe and detaching the syringe from the pipette or taking up further liquid by means of the same syringe is displayed.
  • the display comprises a downward and an upward arrow or other obvious symbols. This facilitates the operation of the pipette.
  • this display comprises a downwardly directed arrow or another obvious symbol. This facilitates the operation of the pipette.
  • a display is only displayed by means of the display device when a syringe is detachably connected to the pipette and / or the display goes out when the syringe is disconnected from the pipette.
  • a battery or other electrical power supply of the pipette is spared.
  • an indication indicative of the setting of the pipette to a lower end position for receiving liquid is displayed when the pipette is not in the lower end position for receiving liquid.
  • the display is according to one embodiment, a downwardly directed arrow or other obvious symbol for the setting of the pipette in the lower end position. This facilitates the operation of the pipette.
  • the newly set metering and the possible without refilling the pipette maximum number of additional metering with the newly set metering volume is displayed and the number of further dosing steps carried out is displayed with the newly set dosing volume by means of the display device.
  • the user receives the information about the set dosing volume, the maximum possible number of further dosing steps and the number of further dosing steps performed.
  • the maximum number of further dosing steps possible without refilling the syringe with the previously set dosing increment by means of the display device and the dosing steps carried out by means of the dosing step width set after the reset or the total dosing steps performed before and after the reset are counted and displayed by means of the display device.
  • the control device determines the possible maximum number of metering steps, which is possible after complete filling of the syringe without refilling the syringe, on the basis of the detected metering step width and data present in the control device about the syringe used.
  • the control device displays the metering volume determined from the metering step width and the determined maximum number of metering steps. When dosages take place, this is signaled to the control device by the means for detecting the execution of a dosing step.
  • the control device counts the metering steps and displays the respectively performed number of metering steps by means of display device.
  • the control device is such that the display of the number of executed metering steps instead of the display of the without refilling the syringe still possible number of dosing steps takes place.
  • the control device is designed such that the display of the number of dosing steps still possible without refilling the syringe takes place in place of the display of the number of dosing steps carried out.
  • the control device is such that the number of metering steps performed and at the same time the number of metering steps still possible without refilling the syringe is displayed.
  • control device is such that one of the two aforementioned numbers indicates greater than the other.
  • the pipette is a pipette driven by the user's muscular strength.
  • the pipette on mechanical drive means for driving the receiving body, which are driven by the muscle power of the user.
  • the pipette has a sensor for detecting a coding indicating the syringe volume on a syringe detachably connected to the pipette.
  • the control device always determines the set metering volume based on the set metering step size and the determined syringe volume and displays it via the display device.
  • the sensor is a ring sensor.
  • the sensor for detecting a coding is according to a preferred embodiment at the same time the means for detecting a syringe detachably connected to the pipette.
  • control device is such that, after carrying out the maximum number of dosing steps and Refilling the same syringe determines the total number of metering steps performed with the syringe with the set Dosier suitsweite and / or determines the number of possible without refilling the syringe still possible metering and displayed by the display device.
  • control device is designed such that, before the liquid is taken up by means of the syringe detachably connected to the pipette, it indicates, by means of the display device, a display indicative of the picking up of liquid.
  • control device controls this display when the means for detecting a syringe signal that a syringe is detachably connected to the pipette before the means for detecting the execution of a dosing step signal a dosing step.
  • the control device is designed so that it indicates a flashing or otherwise indicative of the current process of receiving liquid in the syringe and performing a reverse stroke when recording liquid until complete execution of a reverse stroke by means of the display.
  • the pipette according to one embodiment comprises means for detecting a displacement of the receiving body in a lower end position for releasable connection with a syringe and means for detecting a displacement of the receiving body in an upper end position for the full filling of a syringe, said means for detecting are connected to the electronic control device. These means for detecting signal to the controller that a syringe has been completely filled.
  • the execution of the reverse stroke of the control means of the means for detecting the implementation of a Metering step are signaled if the reverse stroke is controlled by the control knob.
  • means for detecting a reverse stroke are present, which detect the operation of a transfer member for the reverse stroke and are connected to the electronic control device.
  • the transfer member for the reverse stroke is coupled to the receiving body, so that it is taken at the end of the displacement of the receiving body in the upper end position for the full filling of the syringe and taken in the reverse stroke in the opposite direction and are the means for detecting the Displacement of the receiving body in the upper end position at the same time the means for detecting the Umledgehubes.
  • These means for detecting are formed according to an embodiment by a single microswitch, which is switched by the transfer member for the reverse stroke in reaching the position for the full filling of the syringe and after execution of the reverse stroke in different switching states.
  • control device is designed such that it indicates the set dosing volume and the number of dosing steps taken after complete execution of the reverse stroke by means of the display device.
  • the execution of the reverse stroke is signaled to the control device by the means for detecting the execution of a dosing step or by the means for detecting the execution of a reverse stroke.
  • the execution of a dosing step is signaled to the control device by the means for detecting the execution of a dosing step.
  • the controller counts the dosing steps performed and displays them.
  • control device is designed such that it indicates, after complete execution of the reversal stroke by means of the display device indicative of the dispensing of liquid.
  • the execution of the reverse stroke is signaled to the control device by the means for detecting the execution of a dosing step or by the means for detecting the execution of a reverse stroke.
  • control device is designed such that, after the maximum number of metering steps have been carried out by the display device, it indicates an indication of the optional dispensing of remaining liquid from the syringe and the detachment of the syringe from the pipette or the taking up of further liquid by means of the same syringe displays.
  • control device is designed such that it determines the execution of the maximum number of metering steps by comparing the number of metering steps performed with the determined maximum number of metering steps for emptying the syringe.
  • the control device is designed such that, after dispensing residual liquid from the syringe by means of the display device, it indicates an indication of the release of the syringe.
  • the delivery of residual liquid from the syringe is signaled to the controller by the means for detecting the displacement of the receiving body to a lower end position for insertion of the syringe. In the lower end position, the receiving body is displaced as far as possible for receiving the syringe barrel.
  • the control device then brings this information to the display when previously dosing has taken place. This can be signaled to the control device by the means for detecting the execution of a dosing step.
  • control device is such that after the detachable connection of the pipette to the syringe and before the liquid is taken up into the syringe by means of the display device, a display indicative of the setting of the pipette into a lower end position for receiving liquid is displayed when the pipette is not in the initial position for picking up liquid. This is signaled to the pipette by the means for detecting the placement of the receiving body in the lower end position for insertion of a syringe.
  • the control device is such that after performing at least one dosing step and before carrying out the possible maximum number of dosing in a change in the setting of Dosier intimidweite means of the display the newly set dosing and the possible without refilling the syringe maximum number of additional dosing be displayed with the newly set dosing and determined the number of further dosing performed with the newly set dosing and displayed by the display device.
  • the change in the setting of the dosing increment is signaled to the control device by the means for detecting the set dosing increment.
  • the number of further metering steps is determined by the control device on the basis of available data of the syringe volume and the number of metering steps taken with the previously set metering step size.
  • control device is such that, after changing the metering step size and subsequent resetting of the metering step width to the previously set value, the maximum number of further metering steps possible without refilling the syringe with the previously set dosing increment by means of the display means and the means of the re-set Dosier intimide after resetting performed dosing counts and displays by means of the display or counts before and after the reset total dosing and displays by means of the display device.
  • the control device when the dosing increment is changed, stores the previously set value of the dosing step width and the number of dosing steps performed therewith.
  • control device is designed so that it indicates in the case of performing dosing steps after incomplete fluid intake into the syringe by means of the display device indicative of the execution of a reverse stroke display until the reversal stroke is performed by one or more dosing steps on it the following dosing counts and indicates by means of the display.
  • the incomplete fluid intake is signaled to the pipette in that the means for detecting dosing steps signal the execution of dosing steps, without the means for detecting a displacement of the receiving body in the upper end position signaling the displacement of the receiving body in the upper end position.
  • the control device can make the number of required metering steps for performing a reverse stroke as a function of the set metering step size, which is signaled to the control device by the means for detecting the metering step.
  • the control device accesses a stored table in which the number of metering steps for carrying out a reversal stroke is specified as a function of the setting of the metering step width.
  • the means for detecting are preferably carried out in one or more of the following manners: microswitch, Hall sensor with magnet, reed contact with magnet, light barrier, pressure sensor (for example foil pressure sensor), capacitive sensor or printed circuit board with a meandering conductor track actuated by a carbon switching pill on a flex foil.
  • the control device is preferably a microcontroller.
  • the means for holding the attachment portion in the receptacle and the further attachment portion in the further receptacle are preferably formed as described in the above-mentioned prior art documents.
  • the means for displacing the receiving body within the housing are preferably formed as described in the prior art documents mentioned above.
  • the sensor for detecting a coding of a syringe and the coding of the syringe are preferably designed as described in the prior art documents mentioned above. In this regard, reference is made to the documents mentioned above DE 29 26 691 C2 . US 4,406,170A . DE 44 37 716 C2 . EP 0 679 439 B1 . US 5,591,408A . EP 056 229 B1 . US 5,620,660 A . EP 1 724 020 B1 . US 7,731,908 B2 . EP 0 657,216 B1 . US 5,620,661 A , the contents of which are hereby incorporated into the present application.
  • top and bottom refer to the orientation of the pipette, in which the rod-shaped housing is vertically aligned and the receptacle for the syringe is arranged below.
  • a pipette 1 has a rod-shaped housing 2 in which a syringe 3 is held at the bottom. From a side wall of the housing 2 is above a straight line slot 4, an elevator lever 5 of the housing 2 before. From the same side wall of the housing is about two further slots 6, 7 before a control knob 8 a rack-pawl control.
  • a display device in the form of an LCD display 9 is embedded in the same side wall of the housing 2. From openings in the adjacent side wall are segments of a dial 10 out.
  • the syringe 3 has a cylinder 11 and a piston 12 arranged displaceably therein.
  • the cylinder 11 has at the bottom a conical portion 13 with a hole 14 for the passage of liquids and above a cylindrical portion 15 in which the piston 12 is displaceable.
  • the cylinder 11 has a mounting portion 16 with a circumferential flange 17.
  • From the piston 12 is upwardly a piston rod 18, which has a further mounting portion 19 with a plurality of circumferential beads (not shown).
  • the syringe 3 is arranged with the flange 17 in a receptacle 20 at the lower end of the housing 2, which at the lower end of the housing 2 has an axially directed opening 21.1 for insertion and removal of the syringe 3.
  • the syringe 3 presses with its upper side against a pressure-sensitive ring sensor 22, which scans projections on the upper edge of the flange 17.
  • the flange 17 is held in this position by means of gripping levers 23 in the housing 2.
  • the further attachment portion 19 of the piston 12 is arranged in a further receptacle 24 in a hollow cylindrical receiving body 25. This has for insertion of the attachment portion 19 on a further axially directed opening 21.2.
  • the further attachment portion 19 is held by means of further gripping levers 26 which engage between the beads of the further attachment portion 19 or pinch it.
  • the receiving body 25 is fixedly connected to a rack 27 which extends below the slot 4 in the longitudinal direction of the housing 2.
  • An elevator lever holder 28 is fixed to the receiving body 25 and to a lower part of the rack 27.
  • an elevator lever carrier 29 is present, which abuts with a sliding plate 30 at the bottom of the edges of the slot 4.
  • the elevator lever carrier 29 has an upwardly projecting post 31, which passes through the slot 4. On the post outside the housing 2 of the elevator lever 5 is fixed.
  • a metering lever 34 is pivotally mounted in a swivel bearing 32 in a bulge 33 of the slot 4 opposite side wall of the housing 2.
  • the metering lever 34 has two spaced apart legs 35, 36 which protrude on the opposite side wall of the housing 2 from the two slots 6, 7. There, the control knob 8 is fixed on the protruding ends of the legs 35, 36.
  • a pawl 37 is pivotally mounted between the two legs 35, 36 of the metering lever 34.
  • the pawl 37 is arranged with a ratchet tooth 38 above the toothing 39 of the rack 27.
  • the metering lever 34 is moved by a spring device not shown in the position of Fig. 2 pressed. Contrary to the action of the spring device of the metering lever 34 by pressing the control knob 8 is pivoted downwards.
  • the pawl 37 is pressed by means of a further spring means not shown to the teeth 39 of the rack 27.
  • a sliding cover 40 is arranged between the pawl 37 and the rack 27, a sliding cover 40 is arranged.
  • the cover 40 is made by turning the side of the housing 2 protruding selector wheel 10 displaced so that it covers the teeth 39 of the rack 27 more or less.
  • a circuit board 41 is arranged with an electronics.
  • an electrical power supply in the form of batteries or batteries 42.
  • the dial 10 is associated with a further sensor 43 which detects the rotational position of the dial 10.
  • the measured values determined by the ring sensor 22 and the further sensor 43 are forwarded to the electronics via cables.
  • the code indicated on the flange 17 designates the size of the respective syringe 3.
  • the electronics determine the respective syringe size from the measuring signals supplied by the ring sensor 22 and the respective step size from the setting of the dial 10. From this, it calculates the set dosing volume and brings it to display 9 for display.
  • the slots 6, 7 are covered on the inside by a diaphragm 44 connected to the metering lever 34.
  • a flexible cover strip 45 is present.
  • the stake strip 45 is made of polypropylene.
  • Fig. 8 has the cover strip 45 at the ends of eyes 46, 47, according to Fig. 2 are fixed to pins 48, 49 projecting from the inside of the side wall of the housing 2, which has the slot 4.
  • the cover strip 45 extends through a channel 50 between the elevator lever holder 28 and the elevator lever carrier 29.
  • the channel 50 is bounded on opposite sides by a side surface 51 with a radius of the elevator lever holder 28 and by a further side surface 52 with a corresponding radius of the elevator lever carrier 29.
  • the two lateral boundaries of the channel 50 are formed by two strip-shaped side walls 53, 54 of the elevator lever carrier 29. These are connected to each other at a distance from the side surface 52 having the radius by a crossbar 55. Between the radius having further side surface 52 and the cross member 55, the elevator lever carrier has an opening 56.
  • the elevator lever carrier 29 is preassembled with the cover strip 45 by the cover strip 45 is guided along the other side surface 52 and through the aperture 56 so that it extends on the top of the crossbar 55 between the two strip-shaped side walls 53, 54.
  • the elevator lever carrier 29 is connectable to the elevator lever holder 28 by the projections 57, 58 are inserted into the pockets 59, 60 and elevator lever carrier 29 and elevator lever holder 28 are glued together or snapped together by means of suitable snap means.
  • the elevator lever holder 28 and the elevator lever carrier 29 together form a connecting element 62 between the receiving body 25 and the elevator lever 5 (cf. Fig. 2 . 3 . 4 ).
  • the elevator lever mount 28 has wings 63, 64 projecting from both sides.
  • the wings 63, 64 have a cylindrical shape. They engage in grooves 65, 66 on two opposite side walls of the housing 2.
  • the grooves 65, 66 are bounded on one side by an edge of a housing half and on the other side by a bar of a chassis in which parts of the drive mechanism of the pipette 1 are mounted.
  • Fig. 2 is the cover strip 45 of the two pins 48, 49 to the eyes 46, 47 according to Fig. 8, 9 held clamped on the inside of the slot 4.
  • Fig. 3 it is due to the radii of the side surfaces 51, 52 of the channel 50 on the level in which it is extended on both sides of the channel 50, bulged. As a result, a close contact of the cover strip 45 is achieved at the edges of the slot 4.
  • Fig. 10 and 11 carries the circuit board 41 on the side facing away from the rack 27 side at its lower end designed as a microswitch upper limit switch 67 for detecting the upper end position of the receiving body 25.
  • the upper limit switch 67 is a button with a respect to the circuit board 41 outwardly projecting lever 68th
  • the upper limit switch 67 also serves to detect a reverse stroke.
  • a transmission member 69 is arranged for controlling a Umledgehubes in the housing of the pipette.
  • the transmission member 69 has a strip-shaped transmission section 70, which is guided parallel to the rack 27 in the housing of the pipette and displaced.
  • the strip-shaped transfer section 70 has a lower transfer member end 71, which is at a displacement of the transmission member 69 down in the displacement region of the side of the rack 27 projecting lower stop 72 is located.
  • the transmission member 69 has above two control arms 73, 74, in the (zu Fig. 10 and 11 vertical) plane of the circuit board 41 are extended and engage around the lower end of the circuit board 41 on both sides.
  • the transmission member 69 is displaceable so that the control arms 73, 74 reach upper control arm ends 75, 76 in the displacement region of bevelled stop edges 77, 78 on the legs 35, 36 (see. Fig. 10 to 13 ).
  • the transmission member 69 has a switching arm 79 above, which engages over the circuit board 41 on the side at a distance on which the upper limit switch 67 is arranged.
  • the switching arm 79 is designed so that it actuates the sensing lever 68 of the upper limit switch 67 with its upper Druckarmende 80 upon displacement of the transmission member 69 and releases.
  • the switching arm 79 actuates the upper limit switch 67, wherein the sensing lever 68 is covered by the leg 35 of the metering lever 34.
  • Fig. 11 It is shown how the switching arm 79 releases the sensing lever 68 of the upper limit switch 67.
  • the circuit board 41 carries according Fig. 12 and 13 at its lower end on the side facing the rack 27, designed as a microswitch lower limit switch 81.
  • the lower limit switch 81 is a button with a rack 27 projecting towards the sensing lever 82.
  • the sensing lever 82 is located in the displacement of one of the opposite side of the rack 27 projecting upper stop 83. In FIG. 12 gives the upper stop 83, the sensing lever 82 free and in Fig. 13 he pushes him.
  • Fig. 14 to 16 is arranged on the circuit board 41 next to the upper limit switch 67 a Dosier suitssensor 84 for detecting dosing steps.
  • the metering step sensor 84 is also a button.
  • the feeler is preferably a sheet metal membrane which is deformable from a stable, outwardly curved initial position into an unstable, inwardly curved position in which it closes a circuit. After relieving the sheet membrane automatically returns from the unstable to the stable position in which the circuit is open.
  • the metering step sensor 84 is located with the membrane in the pivoting range of a pin 85 made of silicone, which is arranged on a leg 35, 36 of the metering lever 34 connecting and mounted on the pivot bearing 32 Dosierhebelbasis 86 of the metering lever 34.
  • the circuit board 41 carries an electronic control device 87 which is wired to the ring sensor 22, the further sensor 43 for detecting the rotational position of the dial 10, the upper limit switch 67, the lower limit switch 81, the Dosier suitssensor 84 and the display 9. Furthermore, the control device 87 is connected to the battery 42.
  • a syringe 3 with a syringe size selected by the user is releasably connected to the pipette by being inserted into the receptacle 20 in the attachment section 16 and into the receptacle 24 with the attachment section 19, so that the flange 22 of the gripping levers 23 and the attachment section 19 is gripped by the gripping levers 26.
  • the ring sensor 22 scans the coding on the flange 17 of the syringe 3.
  • the control device 87 determines from the signals supplied by the ring sensor 22 that a syringe 3 is inserted and switches the display 9 on. Based on the signals supplied by the ring sensor 22 and the signals supplied by the sensor 43, the control device 87 determines the set metering volume and displays it on the display 9. Optionally, the user changes the setting of the metering volume by means of the dial 10 and the changed metering volume is displayed by the display 9.
  • control device 87 determines the possible maximum number of metering steps for emptying the syringe 3 after complete filling and displays this. Furthermore, the control device 87 by an upward arrow on the display 9 indicates that liquid can be received in the syringe 3.
  • the elevator lever 5 is moved out of the position of Fig. 1 . 2 and 13 pushed up.
  • the upper stop 83 operates according to Fig. 13 the sensing lever 82 of the lower limit switch 81.
  • the upper stop 83 releases the lower limit switch 81, as shown in FIG Fig. 12 will be shown.
  • this signals the lower limit switch 81 of the controller 87, this controls a flashing of the display on the display.
  • a reverse stroke must be carried out. This is done by pressing the control knob 8. Here hit the stop edges 74, 78 on the control arms 73, 74 and move the transmission member 69 down. The transmission member 69 takes over the lower stop 72 with the rack 27. This reverse stroke removes the lots from the system.
  • the switching arm 79 is released from the sensing lever 68 of the upper limit switch 67, whereby the control device 87 is signaled that the reversal stroke has been performed.
  • the display 9 stops flashing and indicates that zero dosing steps have been performed. Furthermore, the display 9 indicates by indicating a downward arrow that liquid can be dispensed.
  • the absorbed liquid amount can be delivered in small steps by the control knob 8 is pressed repeatedly against the action of the spring device down.
  • the further spring device presses the pawl 37 with the pawl tooth 38 against the cover 40 until the pawl tooth 38 reaches the lower end of the cover 40.
  • the pawl 37 falls with the pawl tooth 38 in the toothing 39 of the rack 27 and takes the rack 27 during further pivoting of the metering lever 34 down with a piece.
  • the displacement of the rack 27 depends on each pivoting of the metering lever 34 until reaching a lower stop of a set by means of the dial 10 position of the cover 40 from.
  • To Relief of the control knob 8 he is pushed by the spring device up and another dosing can be performed.
  • the pin 85 hits the dosing step sensor 84 and actuates it (cf. Fig. 14 ). This signals the control device 87 to carry out a dosing step.
  • the control device 87 adds the dosing steps carried out and displays the respective number of dosing steps carried out on the display 9.
  • the control device 87 then indicates on the display 9 that the maximum number of metering steps has taken place. It also indicates by indicating an upward and downward arrow on the display 9 the indication that either liquid can be sucked into the syringe 3 again or empties the residual liquid from the syringe 3 and the syringe 3 separated from the pipette 1 can be. If additional liquid is absorbed into the same syringe 3, the sequence is as described above, wherein the controller 87 determines the sum of the dosing performed with the same dosing and displays on the display 9.
  • a residual liquid in the syringe 3 can be discharged by displacing the elevator lever 5 down.
  • the lower limit switch 81 is actuated.
  • the controller 87 shows on the display 9 a downward Arrow on, as an indication that the syringe 3 can be separated from the pipette 1.
  • the user actuates the handle lever 23, 26th
  • Fig. 17 The procedure described above is in Fig. 17 illustrated.
  • the respective display is indicated in the display and with explanations.
  • the example shows a set dosing volume of 500 ml.
  • Fig. 18 The expiration of Fig. 18 is different from the expiration of Fig. 17 in that the elevator lever 5 is not in the lower end position when releasably connecting the pipette 1 to the syringe 3.
  • This can be detected by the control device 87 that the ring sensor 22 reports the onset of a syringe 3 and the lower limit switch 81 reports that the receiving body 25 is not in the lowest end position.
  • the display on the display device 9 by a downward arrow to indicate to shift the elevator lever 5 down. Only when this is done, the set dosing volume, the maximum number of metering steps and the upward arrow is displayed as an indication of the filling of the syringe 3.
  • the further procedure corresponds to that of Fig. 17 ,
  • Fig. 19 The expiration of Fig. 19 is different from the expiration of Fig. 17 in that during dispensing the setting of the dosing volume is changed.
  • the dosing volume is adjusted to 250 ml.
  • the newly set dosing volume is displayed by means of the display device 9 and the amount of liquid present with this dosing volume and the amount of liquid still present in the syringe 3 possible maximum number of further dosing steps. In the example, these are 8 more steps.
  • the dosing steps carried out with the newly set dosing volume are counted and displayed.
  • Fig. 20 The expiration of Fig. 20 is different from the expiration of Fig. 19 in that, after the change in the dosing volume, the dosing volume is reset to the original value.
  • the metering volume is changed after a metering step of 500 ml to a metering volume of 250 ml. After a metering step with a metering volume of 250 ml, the metering volume is reset to the original metering volume of 500 ml. Thereafter, the with the original metering volume and the displayed in the syringe amount of liquid possible maximum number of additional dosing steps.
  • the dosing steps that have taken place after returning to the original dosing quantity to the original value with this dosing quantity are counted and displayed.
  • the dosing steps taken in total before and after the reset are counted and displayed when the dosing quantity is reset to the originally set value during dispensing.
  • Fig. 21 The expiration of Fig. 21 is different from the expiration of Fig. 17 in that the syringe 3 is only partially filled.
  • This is determined by the controller 87 based on the signals of the lower limit switch 81 and the upper limit switch 67. Namely, when the lower limit switch 81 indicates that the receiving body 25, the lower end position has left and then the dosing counter reports the execution of a dosing without the upper limit switch 67 reports reaching the upper end position, the syringe has been only partially filled.
  • the control device 87 then outputs via the display 9 a flashing display.
  • the flashing display also shows the set dosing volume, the possible maximum number of dosing steps and the arrow pointing upwards. The flashing of the display indicates to the user that there is a partial filling so that reaching the maximum number of dosing steps is not ensured.
  • the controller 87 stops flashing the display only when the user has performed a sufficient number of dosing steps that correspond to a Umledgehub. This indicates to the user that no precise dosing has taken place and he should discard the dispensed doses. As soon as the reversing stroke has been carried out, the control device 87 outputs via the display device 9 the set metering volume, the number of metering steps that have taken place and an arrow indicating the displacement of the receiving body 25 downwards.
  • the control device 87 takes the number of required dosing for the realization of a reverse stroke for each set dosing a stored table.
  • An example of such a table is in Fig. 22 specified.
  • Fig. 17 to 21 can in particular by means of the pipette according to Fig. 1 to 16 be performed.
  • the processes of Fig. 1 to 17 can also be carried out by means of other suitable pipettes.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
EP12002849.3A 2012-04-23 2012-04-23 Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides Active EP2656916B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP12002849.3A EP2656916B1 (fr) 2012-04-23 2012-04-23 Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides
PL12002849T PL2656916T3 (pl) 2012-04-23 2012-04-23 Sposób dozowania cieczy za pomocą pipety i strzykawki oraz pipeta do uruchamiania strzykawki do dozowania cieczy

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP12002849.3A EP2656916B1 (fr) 2012-04-23 2012-04-23 Procédé de dosage de liquide au moyen d'une pipette et d'une seringue ainsi que la pipette destinée à actionner une seringue pour le dosage de liquides

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EP2656916A1 true EP2656916A1 (fr) 2013-10-30
EP2656916B1 EP2656916B1 (fr) 2019-01-16

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EP (1) EP2656916B1 (fr)
PL (1) PL2656916T3 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012011938A1 (de) 2012-06-18 2013-12-19 Eppendorf Ag Pipette zum Betätigen einer Spritze
DE102014109345A1 (de) 2014-07-04 2016-01-07 Eppendorf Ag Pipette zum Betätigen einer Spritze
EP3335795A1 (fr) 2016-12-16 2018-06-20 Eppendorf AG Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides

Citations (10)

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DE2926691C2 (de) 1979-07-02 1983-05-26 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Repetierpipette
EP0056229B1 (fr) 1981-01-13 1985-07-03 Ruhrchemie Aktiengesellschaft Procédé de préparation de catalyseurs sur support pour la polymérisation d'alpha-oléfines
EP0657216A2 (fr) * 1993-12-10 1995-06-14 Eppendorf-Netheler-Hinz Gmbh Système de pipette
DE4414760C1 (de) * 1994-04-27 1995-08-24 Eppendorf Geraetebau Netheler Repetierpipette
DE4437716C2 (de) 1994-10-21 1996-10-17 Eppendorf Geraetebau Netheler Repetierpipette
US5620660A (en) 1993-12-03 1997-04-15 Eppendorf-Netheler-Hinz Gmbh Pipette system
US6254832B1 (en) * 1999-03-05 2001-07-03 Rainin Instrument Co., Inc. Battery powered microprocessor controlled hand portable electronic pipette
EP1524035A1 (fr) * 2003-10-17 2005-04-20 Biohit Oyj Procédé de fixation des pointes de pipette et dispositif pour ce procédé
EP1632840A1 (fr) * 2004-09-07 2006-03-08 Gilson Sas Pipette comportant une roue de commande
EP1724020B1 (fr) 2005-05-20 2010-03-10 Eppendorf Ag Pipette

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US4406170A (en) 1979-07-02 1983-09-27 Eppendorf Geratebau Netheler & Hinz Gmbh Repeating pipetting device
DE2926691C2 (de) 1979-07-02 1983-05-26 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Repetierpipette
EP0056229B1 (fr) 1981-01-13 1985-07-03 Ruhrchemie Aktiengesellschaft Procédé de préparation de catalyseurs sur support pour la polymérisation d'alpha-oléfines
US5620660A (en) 1993-12-03 1997-04-15 Eppendorf-Netheler-Hinz Gmbh Pipette system
EP0656229B1 (fr) 1993-12-03 1997-07-16 Eppendorf-Netheler-Hinz Gmbh Système de pipette
EP0657216A2 (fr) * 1993-12-10 1995-06-14 Eppendorf-Netheler-Hinz Gmbh Système de pipette
EP0657216B1 (fr) 1993-12-10 1999-05-12 Eppendorf-Netheler-Hinz Gmbh Système de pipette
US5620661A (en) 1993-12-10 1997-04-15 Eppendorf-Netherler-Hinz Gmbh Pipette system
DE4414760C1 (de) * 1994-04-27 1995-08-24 Eppendorf Geraetebau Netheler Repetierpipette
US5591408A (en) 1994-04-27 1997-01-07 Eppendorf-Netheler-Hinz Gmbh Repetition pipette
EP0679439B1 (fr) 1994-04-27 1998-03-04 Eppendorf-Netheler-Hinz Gmbh Pipette de distribution à répétition
DE4437716C2 (de) 1994-10-21 1996-10-17 Eppendorf Geraetebau Netheler Repetierpipette
US6254832B1 (en) * 1999-03-05 2001-07-03 Rainin Instrument Co., Inc. Battery powered microprocessor controlled hand portable electronic pipette
EP1524035A1 (fr) * 2003-10-17 2005-04-20 Biohit Oyj Procédé de fixation des pointes de pipette et dispositif pour ce procédé
EP1632840A1 (fr) * 2004-09-07 2006-03-08 Gilson Sas Pipette comportant une roue de commande
EP1724020B1 (fr) 2005-05-20 2010-03-10 Eppendorf Ag Pipette
US7731908B2 (en) 2005-05-20 2010-06-08 Eppendorf Ag Pipette

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012011938A1 (de) 2012-06-18 2013-12-19 Eppendorf Ag Pipette zum Betätigen einer Spritze
DE102014109345A1 (de) 2014-07-04 2016-01-07 Eppendorf Ag Pipette zum Betätigen einer Spritze
US10507463B2 (en) 2014-07-04 2019-12-17 Eppendorf Ag Pipette for activating a syringe
EP3335795A1 (fr) 2016-12-16 2018-06-20 Eppendorf AG Procédé de dosage de liquide au moyen d'une pipette et d'une seringue et pipette destinée à actionner une seringue pour le dosage de liquides
US11154854B2 (en) 2016-12-16 2021-10-26 Eppendorf Ag Method for dosing liquid by means of a pipette and a syringe, and pipette for actuating a syringe for dosing liquid
US11697113B2 (en) 2016-12-16 2023-07-11 Eppendorf Ag Method for dosing liquid by means of a pipette and a syringe, and pipette for actuating a syringe for dosing liquid

Also Published As

Publication number Publication date
EP2656916B1 (fr) 2019-01-16
PL2656916T3 (pl) 2019-07-31

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