EP2532366A1 - Composition pharmaceutique pour le traitement et/ou la prévention du cancer - Google Patents

Composition pharmaceutique pour le traitement et/ou la prévention du cancer Download PDF

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Publication number
EP2532366A1
EP2532366A1 EP11739881A EP11739881A EP2532366A1 EP 2532366 A1 EP2532366 A1 EP 2532366A1 EP 11739881 A EP11739881 A EP 11739881A EP 11739881 A EP11739881 A EP 11739881A EP 2532366 A1 EP2532366 A1 EP 2532366A1
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Prior art keywords
antibody
cancer
caprin
seq
amino acid
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German (de)
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EP2532366B1 (fr
EP2532366A4 (fr
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Shinichi Kobayashi
Fumiyoshi Okano
Takanori Saito
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Toray Industries Inc
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Toray Industries Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/39558—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against tumor tissues, cells, antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]

Definitions

  • the present invention relates to a novel pharmaceutical use of an antibody against CAPRIN-1 or a fragment thereof, as an agent, for treating and/or preventing a cancer.
  • Cancer is the leading cause of death.
  • conducted therapy comprises mainly surgical therapy in combination with radiation therapy and chemotherapy.
  • cancer treatment results have not been much improved recently, excluding that for some types of cancer.
  • Recent advances in molecular biology or cancer immunology lead to identification of antibodies specifically reacting with cancer, cancer antigens to be recognized by cytotoxic T cells, genes encoding cancer antigens, and the like.
  • Demands on specific cancer therapies targeting cancer antigens are increasing (Non-patent Literature 1).
  • Non-patent Literature 2 In cancer therapy, it is desirable that peptides, polypeptides, or proteins recognized as antigens be almost absent in normal cells, but they be present specifically in cancer cells, in order to alleviate side effects.
  • Boon et al. (Ludwig Institute for Cancer Research, Belgium) isolated a human melanoma antigen MAGE1 recognized by CD8-positive T cells by the cDNA expression cloning method using autologous cancer cell lines and cancer-reactive T cells (Non-patent Literature 2).
  • Non-patent Literature 3 and Patent Literature 1 SEREX (serological identification of antigens by recombinant expression cloning) method that comprises identifying tumor antigens recognized by antibodies that are produced in vivo in response to autologous cancer of a cancer patient by gene expression cloning techniques was reported (Non-patent Literature 3 and Patent Literature 1). With the use of this method, some cancer antigens, which are almost never expressed in normal cells but are specifically expressed in cancer cells, were isolated (Non-patent Literatures 4-9). Furthermore, clinical trials were conducted with cell therapies targeting some cancer antigens using immunocytes specifically reactive with cancer antigens, or cancer-specific immunotherapies using vaccines or the like containing cancer antigens.
  • Cytoplasmic- and proliferation-associated protein 1 (CAPRIN-1) is expressed when normal cells at the resting phase are activated or undergo cell division, and it is an intracellular protein known to form intracellular stress granules with RNA within cells, so as to be involved in mRNA transport and translational regulation.
  • CAPRIN-1 many other names that represent CAPRIN-1 exist, such as GPI-anchored membrane protein 1 or membrane component surface marker 1 protein (M11S1), as if such proteins had been known to be cell membrane proteins. These names originated from a report that the gene sequence of CAPRIN-1 is a membrane protein having a GPI-binding region and expressed in colorectal cancer cells (Non-patent Literature 10).
  • Patent Literatures 2 and 3 describe that CAPRIN-1 (as a cell membrane protein) under the name of M11S1 can be used as a target of an antibody medicine in cancer therapy, although working examples do not describe treatment using an antibody against the protein.
  • Non-patent Literature 11 it has been commonly believed from the time of the filing of Patent Literature 2 to date that CAPRIN-1 is not expressed on the surface of a cell.
  • the contents of Patent Literatures 2 and 3 based only on incorrect information that CAPRIN-1 is a cell membrane protein should not clearly be understood as common general knowledge for persons skilled in the art.
  • Objects of the present invention are to identify a cancer antigen protein specifically expressed on the surface of a cancer cell and to provide the use of an antibody targeting the cancer antigen protein as an agent for treating and/or preventing a cancer.
  • the present inventors have now obtained a cDNA encoding a protein that binds to an antibody existing in sera from dogs with breast cancer by the SEREX method using both cDNA libraries prepared from dog testis tissues and sera of dogs with breast cancer.
  • the present inventors have now further prepared CAPRIN-1 proteins having the even-numbered amino acid sequences of SEQ ID NOS: 2 to 30 and antibodies against such CAPRIN-1 proteins based on the obtained dog gene and the corresponding human, cattle, horse, mouse, and chicken homologous genes.
  • CAPRIN-1 is specifically expressed in breast cancer, brain tumor, leukemia, lymphoma, lung cancer, uterine cervix cancer, bladder cancer, esophageal cancer, colorectal cancer, gastric cancer, renal cancer, ovarian cancer, prostate cancer, and fibrosarcoma, and that a portion of the CAPRIN-1 protein is specifically expressed on the surface of each cancer cell.
  • the present inventors have thus now found that an antibody or antibodies against the portion of CAPRIN-1 expressed on the surface of each cancer cell is/are cytotoxic to the CAPRIN-1-expressing cancer cells. On the basis of these findings, the present invention as described below was completed.
  • the present invention has the following characteristics.
  • the present invention provides a pharmaceutical composition for treating and/or preventing a cancer, comprising an antibody or a fragment thereof as an active ingredient having immunological reactivity with a partial polypeptide of CAPRIN-1, wherein CAPRIN-1 is represented by any of the even-numbered sequences of SEQ ID NOS: 2 to 30, and wherein the partial polypeptide comprises the amino acid sequence represented by SEQ ID NO: 37 or an amino acid sequence having 80% or more sequence identity with the amino acid sequence of SEQ ID NO: 37.
  • the above caner is breast cancer, brain tumor, leukemia, lymphoma, lung cancer, uterine cervix cancer, bladder cancer, esophageal cancer, colorectal cancer, gastric cancer, renal cancer, ovarian cancer, prostate cancer, or fibrosarcoma.
  • the antibody is a monoclonal antibody or a polyclonal antibody.
  • the antibody is a human antibody, a humanized antibody, chimeric antibody, single chain antibody, or bispecific antibody.
  • the antibody against CAPRIN-1 used in the present invention is cytotoxic to cancer cells. As such, the antibody against CAPRIN-1 is useful for treating or preventing cancers.
  • the anti-tumor activity of an antibody against a polypeptide represented by any of the even-numbered sequences of SEQ ID NOS: 2 to 30 used in the present invention can be evaluated by examining in vivo suppression of tumor growth in animals with cancer, or, examining whether or not the antibody exhibits cytotoxicity via immunocytes or complements to tumor cells expressing the polypeptide in vitro, as described later.
  • nucleotide sequences of polynucleotides encoding proteins comprising the even-numbered amino acid sequences (i.e., SEQ ID NOS: 2, 4, 6, ..., 28, 30) of SEQ ID NOS: 2 to 30 are represented by the odd-numbered sequences (i.e., SEQ ID NOS: 1, 3, 5, ... , 27, 29) of SEQ ID NOS: 1 to 29.
  • amino acid sequences that are represented by SEQ ID NOS: 6, 8, 10, 12, and 14 in the Sequence Listing disclosed herein are the amino acid sequences of CAPRIN-1 isolated as polypeptides, which bind to antibodies specifically existing in serum from a dog with cancer, through the SEREX method using a cDNA library from dog testis tissue and the serum of a dog with breast cancer.
  • the amino acid sequences represented by SEQ ID NOS: 2 and 4 are the amino acid sequences of CAPRIN-1 isolated as human homologues.
  • the amino acid sequence represented by SEQ ID NO: 16 is the amino acid sequence of CAPRIN-1 isolated as a cattle homologue.
  • the amino acid sequence represented by SEQ ID NO: 18 is the amino acid sequence of CAPRIN-1 isolated as a horse homologue.
  • the amino acid sequences represented by SEQ ID NOS: 20 to 28 are the amino acid sequences of CAPRIN-1 isolated as mouse homologues.
  • the amino acid sequence represented by SEQ ID NO: 30 is the amino acid sequence of CAPRIN-1 isolated as a chicken homologue (see Example 1 described later).
  • CAPRIN-1 is known to be expressed when normal cells in the resting phase are activated or give rise to cell division.
  • CAPRIN-1 was not expressed on cell surfaces.
  • an antibody recognizing a partial polypeptide of the CAPRIN-1 protein which comprises the amino acid sequence represented by SEQ ID NO: 37 or an amino acid sequence having 80% or more, preferably 85% or more, more preferably 90% or more, further preferably 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 37, exhibits anti-tumor activity.
  • the antibody of the present invention include all antibodies which bind to a fragment of the above CAPRIN-1 protein and exhibit anti-tumor activity.
  • the above-described anti-CAPRIN-1 antibody used in the present invention may be any type of antibody as long as it can exhibit anti-tumor activity.
  • examples of such antibodies include monoclonal antibodies, polyclonal antibodies, recombinant antibodies, such as synthetic antibodies, multispecific antibodies, humanized antibodies, chimeric antibodies, and single chain antibodies (scFv), human antibodies, and fragments thereof, such as Fab, F(ab') 2 , and Fv. These antibodies and fragments thereof can be prepared by methods known by persons skilled in the art.
  • antibodies having immunological reactivity with CAPRIN-1 proteins or partial polypeptides thereof that is, binding to CAPRIN-1 proteins via antigen-antibody reaction
  • antibodies capable of specifically binding to CAPRIN-1 proteins are desired.
  • they are monoclonal antibodies.
  • Polyclonal antibodies may also be used as long as homogenous antibodies can be stably produced.
  • human antibodies or humanized antibodies are desired in order to avoid or suppress rejection.
  • the term "specifically binding to CAPRIN-1 protein" as used herein means that the antibody specifically binds to a CAPRIN-1 protein, but does not substantially bind to proteins other than the CAPRIN-1 protein.
  • the anti-tumor activity of an antibody that can be used in the present invention can be evaluated as described below by examining in vivo the suppression of the tumor growth in animals with cancer, or, by examining whether or not it exhibits in vitro an activity of cytotoxicity, which is mediated by immunocytes or complements, to tumor cells expressing the polypeptide.
  • examples of the subject for cancer treatment and/or prevention in the present invention include mammals, such as humans, pet animals, domestic animals, and animals for competition.
  • a preferable subject is a human.
  • Proteins or fragments thereof to be used as sensitizing antigens for obtaining anti-CAPRIN-1 antibodies used in the present invention may be derived from any animal species without particular limitation, such as humans, dogs, cattle, horses, mice, rats, and chickens. However, proteins or fragments thereof are preferably selected in consideration of compatibility with parent cells used for cell fusion. In general, mammal-derived proteins are preferred and, in particular, human-derived protein is preferred. For example, when CAPRIN-1 is human CAPRIN-1, the human CAPRIN-1 protein, a partial peptide thereof, or cells expressing human CAPRIN-1 can be used.
  • nucleotide sequences and the amino acid sequences of human CAPRIN-1 and homologues thereof can be obtained by accessing GenBank (NCBI, U.S.A.) and using an algorithm such as BLAST or FASTA ( Karlin and Altschul, Proc. Natl. Acad. Sci. U.S.A., 90: 5873-5877, 1993 ; Altschul et al., Nucleic Acids Res. 25: 3389-3402, 1997 ).
  • a target nucleic acid or a target protein comprises a sequence having 70% to 100%, preferably 80% to 100%, more preferably 90% to 100%, even more preferably 95% to 100% (e.g., 97% to 100%, 98% to 100%, 99% to 100%, or 99.5% to 100%) sequence identity with the nucleotide sequence or the amino acid sequence of the ORF or the mature portion of human CAPRIN-1.
  • % sequence identity refers to a percentage (%) of identical amino acids (or nucleotides) relative to the total number of amino acids (or nucleotides), when two sequences are aligned to achieve the highest similarity with or without introduction of gaps.
  • the length of a fragment of CAPRIN-1 protein ranges from the amino acid length of an epitope (antigenic determinant), which is the minimum unit recognized by an antibody, to a length less than the full length of the protein.
  • epitope refers to a polypeptide fragment having antigenicity or immunogenicity in mammals, preferably in humans, and the minimum unit of the epitope consists of about 7 to 12 amino acids, for example 8 to 11 amino acids.
  • the antibody of the present invention is characterized by recognizing a fragment containing at least an epitope consisting of about 7 to 12 continuous amino acids (e.g., 8 to 11 continuous amino acids) in the amino acid sequence represented by SEQ ID NO: 37 or an amino acid sequence having 80% or more, preferably 85% or more, more preferably 90% or more, further preferably 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 37.
  • the antibody is characterized by binding (preferably, specifically binding) to such a partial sequence (fragment).
  • polypeptides comprising the above-mentioned human CAPRIN-1 protein or partial peptides of the protein, can be synthesized by a chemical synthesis method, such as the Fmoc method (fluorenylmethyloxycarbonyl method) or the tBoc method (t-butyloxycarbonyl method) ( Edited by The Japanese Biochemical Society, Seikagaku Jikken Koza (Biochemical Experimental Lecture Series) 1, Protein Chemistry IV, Chemical Modification and Peptide Synthesis, TOKYO KAGAKU DOZIN (Japan), 1981 ).
  • the above-mentioned polypeptides may also be synthesized by conventional methods using various commercially available peptide synthesizers.
  • a polynucleotide encoding the above polypeptide is prepared and then incorporated into an expression vector, which is subsequently introduced into a host cell in order to produce a polypeptide of interest in the host cell, and then recover it.
  • the polynucleotides encoding the above polypeptides can be easily prepared by known genetic engineering techniques or conventional techniques using a commercially available nucleic acid synthesizer.
  • DNA comprising the nucleotide sequence of SEQ ID NO: 1 can be prepared by PCR using a human chromosomal DNA or cDNA library, as a template, and a pair of primers designed to be able to amplify the nucleotide sequence represented by SEQ ID NO: 1. PCR conditions can be appropriately determined.
  • PCR conditions comprise conducting 30 cycles of the reaction cycle of: denaturation at 94°C for 30 seconds; annealing at 55°C for 30 seconds to 1 minute; and extension at 72°C for 2 minutes, using a thermostable DNA polymerase (e.g., Taq polymerase or Pfu polymerase) and PCR buffer containing Mg 2+ , followed by reacting at 72°C for 7 minutes.
  • a thermostable DNA polymerase e.g., Taq polymerase or Pfu polymerase
  • PCR buffer containing Mg 2+ containing Mg 2+
  • a cDNA library is preferably constructed from cells, organs or tissues, which express proteins having even-numbered sequences of SEQ ID NOS: 2 to 30. Examples of such cells or tissues include cells or tissues derived from testis, and cancers or tumors, such as leukemia, breast cancer, lymphoma, brain tumor, lung cancer, colorectal cancer, and the like.
  • Procedures such as the preparation of probes or primers, construction of a cDNA library, screening of a cDNA library, and cloning of target genes are known by a person skilled in the art and can be carried out by the methods described in Sambrook et al., Molecular Cloning, 2nd Edition, Current Protocols in Molecular Biology (1989 ), Ausbel et al., (above), and the like.
  • DNA encoding a human CAPRIN-1 protein or a partial peptide thereof can be obtained from the thus obtained DNA.
  • the host cells may be any cells, as long as they can express the above-mentioned polypeptide.
  • prokaryotic cells include, but are not limited to, Escherichia coli and the like.
  • eukaryotic cells include, but are not limited to, mammalian cells, such as monkey kidney cells (COS1) and Chinese hamster ovary cells (CHO), human fetal kidney cell line (HEK293), fetal mouse skin cell line (NIH3T3), yeast cells such as budding yeast and fission yeast, silkworm cells, and Xenopus oocyte.
  • an expression vector used herein contains an origin replicable within prokaryotic cells, a promoter, a ribosome-binding site, a multiple cloning site, a terminator, a drug resistance gene, an auxotrophic complementary gene, and the like.
  • Escherichia coli expression vector include a pUC-based vector, pBluescript II, a pET expression system, and a pGEX expression system.
  • DNA encoding the above polypeptide is incorporated into such an expression vector, prokaryotic host cells are transformed with the vector, the thus obtained transformed cells are cultured, and thus the polypeptide encoded by the DNA can be expressed in prokaryotic host cells. At this time, the polypeptide can also be expressed as a fusion protein with another protein.
  • an expression vector used herein is an expression vector for eukaryotic cells, which contains a promoter, a splicing region, a poly(A) addition site, and the like.
  • Examples of such an expression vector include pKA1, pCDM8, pSVK3, pMSG, pSVL, pBK-CMV, pBK-RSV, EBV vector, pRS, pcDNA3, and pYES2.
  • DNA encoding the above polypeptide is incorporated into such an expression vector, eukaryotic host cells are transformed with the vector, the thus obtained transformed cells are cultured, and thus the polypeptide encoded by the DNA can be expressed in eukaryotic host cells.
  • pIND/V5-His, pFLAG-CMV-2, pEGFP-N1, pEGFP-C1, or the like is used as an expression vector, the above polypeptide can be expressed as a fusion protein to which a tag from among various tags such as a His tag (e.g., (His) 6 -(His) 10 ), a FLAG tag, a myc tag, an HA tag, and GFP has been added.
  • a known method for introduction of an expression vector into host cells, a known method can be employed, such as electroporation, a calcium phosphate method, a liposome method, a DEAE dextran method, microinjection, viral infection, lipofection, and binding to a cell membrane-permeable peptide.
  • the polypeptide of interest can be isolated and purified from host cells by a combination of known separation procedures.
  • a denaturing agent such as urea or a surfactant
  • ultrasonication enzymatic digestion
  • salting-out or solvent fractionation and precipitation dialysis, centrifugation, ultrafiltration, gel filtration, SDS-PAGE, isoelectric focusing, ion exchange chromatography, hydrophobic chromatography, affinity chromatography, and reverse phase chromatography.
  • An antibody is a heteromultimeric glycoprotein that generally contains at least two heavy chains and two light chains.
  • Antibodies other than IgM an antibody is an about 150-kDa heterotetramer glycoprotein composed of two identical light (L) chains and two identical heavy (H) chains.
  • L light chain
  • H heavy chain
  • each light chain is connected to a heavy chain via one disulfide covalent bond, however, the number of disulfide bonds between heavy chains of various immunoglobulin isotypes is varied.
  • Each heavy chain or each light chain also has an intrachain disulfide bond.
  • Each heavy chain has a variable domain (VH region) on one end followed by several constant regions.
  • Each light chain has a variable domain (VL region) and has one constant region on an end opposite to the other end.
  • the constant region of a light chain is aligned with the first constant region of a heavy chain, and a light chain variable domain is aligned with a heavy chain variable domain.
  • a specific region of an antibody variable domain exhibits specific variability that is referred to as a complementarity determining region (CDR), so that it imparts binding specificity to the antibody.
  • CDR complementarity determining region
  • a portion of a variable region, which is relatively conserved, is referred to as a framework region (FR).
  • Complete heavy chain and light chain variable domains separately contains four FRs ligated via three CDRs. The three CDRs in a heavy chain are referred to as CDRH1, CDRH2, and CDRH3 in this order from the N-terminus.
  • CDRLs are referred to as CDRL1, CDRL2, and CDRL3.
  • CDRH3 is most important for the binding specificity of an antibody to an antigen. Also, the CDRs of each chain are retained together in a state of being adjacent to each other due to the FR regions, contributing to the formation of the antigen binding site of the antibody together with CDRs from the other chain.
  • a constant region does not directly contribute to the binding of an antibody to an antigen, but exhibits various effector functions, such as involvement in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis via binding to an Fc ⁇ receptor, the rate of half-life/clearance via a neonate Fc receptor (FcRn), and complement-dependent cytotoxicity (CDC) via a C1q constituent of the complement cascade.
  • ADCC antibody-dependent cell-mediated cytotoxicity
  • FcRn neonate Fc receptor
  • CDC complement-dependent cytotoxicity
  • anti-CAPRIN-1 antibody refers to an antibody having immunological reactivity with a full-length CAPRIN-1 protein or a fragment thereof.
  • an antibody used in the present invention may be any type of antibody, as long as it binds to a CAPRIN-1 protein so as to be able to damage tumor, such as leukemia, lymphoma, breast cancer, brain tumor, lung cancer, esophageal cancer, gastric cancer, renal cancer, colorectal cancer, ovarian cancer, prostate cancer, or fibrosarcoma.
  • an antibody examples include a monoclonal antibody, a polyclonal antibody, a synthetic antibody, a multispecific antibody, a human antibody, a humanized antibody, a chimeric antibody, a single chain antibody, and an antibody fragment (e.g., Fab and F(ab') 2 ).
  • an antibody may be an immunoglobulin molecule of any class such as IgG, IgE, IgM, IgA, IgD, or IgY, or any subclass such as IgG1, IgG2, IgG3, IgG4, IgA1, or IgA2.
  • the antibody may further be modified by, in addition to glycosylation, acetylation, formylation, amidation, phosphorylation, pegylation (PEG), or the like.
  • the antibody is a monoclonal antibody
  • the breast cancer cell line SK-BR-3 expressing CAPRIN-1 is administered to a mouse for immunization, the spleen is removed from the mouse, cells are separated, and then the cells and mouse myeloma cells are fused. From among the thus obtained fusion cells (hybridomas), a clone producing an antibody having the effect of suppressing cancer cell proliferation is selected.
  • a hybridoma producing a monoclonal antibody that has the effect of suppressing cancer cell proliferation is isolated, the hybridoma is cultured, and then an antibody is purified from the culture supernatant by general affinity purification, so that the antibody can be prepared.
  • the hybridoma producing a monoclonal antibody can also be prepared as described below, for example.
  • a sensitizing antigen according to a known method.
  • a general method is carried out by injecting a sensitizing antigen to a mammal intraperitoneally or subcutaneously.
  • a sensitizing antigen is diluted with PBS (Phosphate-Buffered Saline), saline, or the like to an appropriate amount, followed by suspension.
  • PBS Phosphate-Buffered Saline
  • saline or the like
  • the resultant is then mixed with an appropriate amount of a general adjuvant as necessary, such as Freund's complete adjuvant.
  • the solution was administered to a mammal several times every 4 to 21 days.
  • an appropriate carrier can also be used upon immunization with a sensitizing antigen.
  • a mammal is immunized as described above. After confirmation of a rise in a desired serum antibody level, immunized cells are collected from the mammal and then subjected to cell fusion. Preferable immunized cells are particularly splenocytes.
  • Mammalian myeloma cells are used as the other parent cells to be fused with the immunized cells.
  • various known cell lines are preferably used, such as P3U1 (P3-X63Ag8U1), P3 (P3x63Ag8. 653) ( J. Immunol. (1979) 123, 1548-1550 ), P3x63Ag8U.1 ( Current Topics in Microbiology and Immunology (1978) 81, 1-7 ), NS-1 ( Kohler. G. and Milstein, C. Eur. J. Immunol. (1976) 6, 511-519 ), MPC-11 ( Margulies. D. H.
  • Fusion of the immunized cell and the myeloma cell can be carried out according to basically a known method such as Kohler and Milstein's technique ( Kohler, G. and Milstein, C. Methods Enzymol. (1981) 73, 3-46 ), for example.
  • the above cell fusion is carried out, for example, in the presence of a cell fusion accelerator in a usual nutrient culture medium.
  • a cell fusion accelerator polyethyleneglycol (PEG), Sendai virus (HVJ), or the like is used.
  • an auxiliary agent such as dimethylsulfoxide may be added and used in order to enhance fusion efficiency.
  • the ratio of the immunized cells to the myeloma cells to be used herein can be arbitrarily set.
  • the number of immunized cells that are preferably used is one to ten times the number of myeloma cells.
  • a culture medium to be used for the above-mentioned cell fusion an RPMI1640 culture medium suitable for proliferation of the above-mentioned myeloma cell line, an MEM culture medium, and other culture media usually used for culturing this kind of cell can be used.
  • liquid that is supplemental to serum such as fetal bovine serum (FCS) can be used together therewith.
  • FCS fetal bovine serum
  • Cell fusion can be performed by thoroughly mixing the predetermined amounts of the above immunized cells and the myeloma cells in the above culture medium, and a PEG solution (for example, having an average molecular weight ranging from about 1000 to 6000) prewarmed at about 37°C is added usually at a concentration of 30%-60% (w/v) and mixed, thereby forming a culture containing hybridomas of interest.
  • a suitable culture medium is successively added to the thus-obtained culture, which is then centrifuged to remove the supernatant, and this procedure is repeated to remove the cell fusion agent or the like which is not preferable for the growth of hybridomas.
  • hybridomas are cultured for selection in a usual selection culture medium (e.g., a HAT culture medium containing hypoxanthine, aminopterin and thymidine). Culturing in this HAT culture medium is continued for a sufficient period of time (usually several days to several weeks) so that the cells (non-fused cells) other than the target hybridomas die. Subsequently, screening and single cloning of the hybridoma which produces an antibody of interest are performed using the general limiting dilution method.
  • a usual selection culture medium e.g., a HAT culture medium containing hypoxanthine, aminopterin and thymidine.
  • hybridomas are obtained by an immunizing non-human animal with an antigen.
  • hybridomas that produce a human antibody having desired activity can also be obtained by in vitro sensitizing human lymphocytes, such as human lymphocytes that have been infected with the EB virus, with a protein, a protein-expressing cell, or a lysate thereof, followed by fusing of the thus sensitized lymphocytes with human-derived myeloma cells having an ability to permanently divide, such as U266 (registration no. TIB 196).
  • the thus prepared hybridoma that produces a monoclonal antibody of interest can be passaged in a general culture medium and can be stored in liquid nitrogen over a long period of time.
  • a hybridoma can be prepared by immunizing by a general immunization method using, as a sensitizing antigen, a desired antigen or a cell that expresses the desired antigen, fusing the thus obtained immunized cell with a known parent cell by a general cell fusion method, and then screening for a monoclonal antibody-producing cell (i.e., a hybridoma) by a general screening method.
  • Another example of an antibody that can be used in the present invention is a polyclonal antibody.
  • a polyclonal antibody can be obtained as described below, for example.
  • a small animal such as a mouse, a human antibody-producing mouse, or a rabbit, is immunized with a natural CAPRIN-1 protein, a recombinant CAPRIN-1 protein expressed in a microorganism such as Escherichia coli in the form of a fusion protein with GST or the like, or a partial peptide thereof, and then serum is obtained.
  • the serum is purified by ammonium sulfate precipitation, protein A column, protein G column, DEAE ion exchange chromatography, affinity column to which a CAPRIN-1 protein or a synthetic peptide has been coupled, or the like, so that a polyclonal antibody can be prepared.
  • a human antibody-producing mouse As a human antibody-producing mouse, a KM mouse (Kirin Pharma/Medarex) and a Xeno mouse (Amgen) are known (e.g., International Patent Publications WO02/43478 and WO02/092812 ), for example.
  • a complete human polyclonal antibody can be obtained from blood.
  • splenocytes are collected from the immunized mouse and then a human-type monoclonal antibody can be prepared by a method for fusion with myeloma cells.
  • An antigen can be prepared according to a method using animal cells ( JP Patent Publication (Kohyo) No. 2007-530068 ) or baculovirus (e.g., International Patent Publication WO98/46777 ), for example.
  • the antigen may be bound to a macromolecule having immunogenicity, such as albumin, and then immunization is carried out.
  • an antibody gene is cloned from said hybridoma and then incorporated into an appropriate vector.
  • the vector is then introduced into a host, and then the genetically recombined antibody produced using gene recombination techniques can be used (e.g., see Carl, A. K. Borrebaeck, James, W. Larrick, THERAPEUTIC MONOCLONAL ANTIBODIES, Published in the United Kingdom by MACMILLAN PUBLISHERS LTD, 1990 ).
  • the cDNA of a variable region (V region) of an antibody is synthesized from the mRNA of the hybridoma using reverse transcriptase.
  • DNA encoding the V region of an antibody of interest When DNA encoding the V region of an antibody of interest can be obtained, this DNA is ligated to DNA encoding the constant region (C region) of a desired antibody, and then the resultant fusion product is incorporated into an expression vector.
  • DNA encoding the V region of an antibody may be incorporated into an expression vector containing the DNA for the C region of an antibody.
  • the DNA can be incorporated into an expression vector so that it is expressed under the control of expression control regions, such as enhancer and promoter.
  • host cells are transformed with the expression vector, so that the antibody can be expressed.
  • the anti-CAPRIN-1 antibody of the present invention is preferably a monoclonal antibody.
  • the anti-CAPRIN-1 antibody may also be a polyclonal antibody or a genetically-modified antibody (e.g., a chimeric antibody or a humanized antibody), for example.
  • Examples of a monoclonal antibody include human monoclonal antibodies, non-human animal monoclonal antibodies (e.g., a mouse monoclonal antibody, a rat monoclonal antibody, a rabbit monoclonal antibody, and a chicken monoclonal antibody), and chimeric monoclonal antibodies.
  • a monoclonal antibody can be prepared by culturing a hybridoma obtained by cell fusion of a splenocyte from a non-human mammal (e.g., a mouse, a human antibody-producing mouse, a chicken, or a rabbit) immunized with a CAPRIN-1 protein, with a myeloma cell.
  • a chimeric antibody is prepared by combining sequences from different animals, such as an antibody comprising heavy chain and light chain variable regions of a mouse antibody and heavy chain and light chain constant regions of a human antibody.
  • a chimeric antibody can be prepared using a known method. For example, a chimeric antibody can be obtained by ligating DNA encoding an antibody V region to DNA encoding a human antibody C region, incorporating the resultant fusion product into an expression vector, and then introducing the vector into a host for production of the chimeric antibody.
  • monoclonal antibodies having immunological reactivity with a partial polypeptide of CAPRIN-1 were prepared, wherein CAPRIN-1 is represented by any of the even-numbered sequences of SEQ ID NOS: 2 to 30, and wherein the partial polypeptide comprises the amino acid sequence represented by SEQ ID NO: 37 or an amino acid sequence having 80% or more sequence identity with the amino acid sequence of SEQ ID NO: 37.
  • the anti-tumor effects of the monoclonal antibodies were confirmed.
  • VH heavy chain variable
  • VL light chain variable
  • the VH region comprises CDR1 represented by the amino acid sequence of SEQ ID NO: 40, 44, or 60
  • CDR2 represented by the amino acid sequence of SEQ ID NO: 41, 45, or 61
  • CDR3 represented by the amino acid sequence of SEQ ID NO: 42, 46, or 62
  • the VL region comprises CDR1 represented by the amino acid sequence of SEQ ID NO: 48 or 64, CDR2 represented by the amino acid sequence of SEQ ID NO: 49 or 65, and CDR3 represented by the amino acid sequence of SEQ ID NO: 50 or 66.
  • Examples of a polyclonal antibody include an antibody obtained by immunizing a human antibody-producing animal (e.g., a mouse) with a CAPRIN-1 protein.
  • a humanized antibody is a modified antibody that is also referred to as a reshaped human antibody.
  • a humanized antibody can be constructed by transplanting CDRs of an antibody from an immunized animal into the complementarity determining regions of a human antibody. General gene recombination techniques therefor are also known.
  • DNA sequences designed to have each of the CDRs of a mouse or chicken antibody ligated to each of the framework regions (FRs) of a human antibody are synthesized by the PCR method from several oligonucleotides, which are prepared so as to have overlap portions at their terminal portions, for example.
  • a humanized antibody can be obtained by ligating the thus obtained DNA to DNA encoding the constant region of a human antibody, incorporating the resultant fusion product into an expression vector, introducing the vector into a host, and thus causing the host to produce the gene product (see European Patent Publication No. 239400 and International Patent Publication WO96/02576 ).
  • FRs of a human antibody which is ligated via CDRs
  • FRs that allow the formation of an antigen-binding site with good complementarity determining regions are selected.
  • the amino acids of the framework regions of an antibody variable region may be substituted ( Sato, K. et al., Cancer Research, 1993, 53: 851-856 ).
  • the amino acids of FRs may be substituted with those of framework regions from various human antibodies (see International Patent Publication WO99/51743 ).
  • FRs framework regions of a human antibody, which are ligated via CDRs
  • FRs that allows the formation of an antigen-binding site with good complementarity determining regions are selected. If necessary, for the formation of an antigen-binding site having the appropriate complementarity determining regions of a reshaped human antibody, the amino acids of the framework regions of an antibody variable region may be substituted ( Sato K. et al., Cancer Research 1993, 53: 851-856 ).
  • amino acids in a variable region e.g., FR
  • a constant region e.g., FR
  • Amino acid substitution is a substitution of, for example, less than 15, less than 10, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, or 2 or less amino acids and is preferably a substitution of 1 to 5 amino acids, and more preferably 1 or 2 amino acids.
  • a substituted antibody should be functionally equivalent to an unsubstituted antibody.
  • Substitution is desirably a substitution of a conservative amino acid(s) between amino acids having analogous properties such as electric charge, side chain, polarity, and aromaticity.
  • Amino acids having analogous properties can be classified into basic amino acids (arginine, lysine, and histidine), acidic amino acids (aspartic acid and glutamic acid), uncharged polar amino acids (glycine, asparagine, glutamine, serine, threonine, cysteine, and tyrosine), nonpolar amino acids (leucine, isoleucine, alanine, valine, proline, phenylalanine, tryptophan, and methionine), branched-chain amino acids (threonine, valine, and isoleucine), and aromatic amino acids (phenylalanine, tyrosine, tryptophan, and histidine), for example.
  • basic amino acids arginine, lysine, and histidine
  • acidic amino acids aspartic acid and glutamic acid
  • uncharged polar amino acids glycine, asparagine, glutamine, serine, threonine, cysteine, and
  • modified antibody product examples include antibodies bound to various molecules such as polyethylene glycol (PEG). Substances to be bound in the modified antibody product of the present invention are not limited. Such a modified antibody product can be obtained by subjecting the thus obtained antibody to chemical modification. Methods therefor have already been established in the art.
  • PEG polyethylene glycol
  • the term "functionally equivalent” refers to that a subject antibody has biological or biochemical activity similar to that of the antibody of the present invention, and specifically refers to that a subject antibody has the function of impairing tumor without essentially causing rejection upon its application to a human, for example.
  • An example of such activity includes an activity to suppress cell proliferation or a binding activity.
  • a method for introducing a mutation into a polypeptide is known.
  • persons skilled in the art can prepare an antibody functionally equivalent to the antibody of the present invention by appropriately introducing a mutation into the antibody using site-directed mutagenesis ( Hashimoto-Gotoh, T. et al., (1995) Gene 152, 271-275 ; Zoller, MJ., and Smith, M. (1983) Methods Enzymol. 100, 468-500 ; Kramer, W. et al., (1984) Nucleic Acids Res. 12, 9441-9456 ; Kramer, W.
  • An antibody that recognizes an epitope of a CAPRIN-1 protein recognized by the above anti-CAPRIN-1 antibody can be obtained by a method known by persons skilled in the art.
  • such an antibody can be obtained by a method that involves determining an epitope of a CAPRIN-1 protein recognized by an anti-CAPRIN-1 antibody, by a general method (e.g., epitope mapping) and then preparing an antibody using a polypeptide having an amino acid sequence contained in the epitope as an immunogen, or a method that involves determining an epitope of such an antibody prepared by a general method, and then selecting an antibody having the epitope identical with that of an anti-CAPRIN-1 antibody.
  • a general method e.g., epitope mapping
  • epitope refers to, in a mammal and preferably a human, a polypeptide fragment having antigenicity or immunogenicity.
  • the minimum size unit thereof consists of about 7 to 12 amino acids, and preferably 8 to 11 amino acids.
  • the affinity constant Ka (k on /k off ) of the antibody of the present invention is preferably at least 10 7 M -1 , at least 10 8 M -1 , at least 5 x 10 8 M -1 , at least 10 9 M -1 , at least 5 x 10 9 M -1 , at least 10 10 M -1 , at least 5 x 10 10 M -1 , at least 10 11 M -1 , at least 5 x 10 11 M -1 , at least 10 12 M -1 , or at least 10 13 M -1 .
  • the antibody of the present invention can be conjugated with an antitumor agent. Conjugation of the antibody with an antitumor agent can be carried out via a spacer having a group reactive to an amino group, a carboxyl group, a hydroxy group, a thiol group or the like (e.g., a succinimidyl succinate group, a formyl group, a 2-pyridyldithio group, a maleimidyl group, an alkoxy carbonyl group, and a hydroxy group).
  • a spacer having a group reactive to an amino group, a carboxyl group, a hydroxy group, a thiol group or the like (e.g., a succinimidyl succinate group, a formyl group, a 2-pyridyldithio group, a maleimidyl group, an alkoxy carbonyl group, and a hydroxy group).
  • antitumor agent examples include the following known antitumor agents as in prior art literatures and the like, such as paclitaxel, doxorubicin, daunorubicin, cyclophosphamide, methotrexate, 5-fluorouracil, thiotepa, busulfan, improsulfan, piposulfan, benzodopa, carboquone, meturedopa, uredopa, altretamine, triethylenemelamine, triethylenephosphoramide, triethilenethiophosphoramide, trimethylolomelamine, bullatacin, bullatacinone, camptothecin, bryostatin, callystatin, cryptophycin1, cryptophycin8, dolastatin, duocarmycin, eleutherobin, pancratistatin, sarcodictyin, spongistatin, chlorambucil, chlornaphazine, cholophosphamide, estramus
  • This technique is applicable to both before and after surgery of a cancer patient with the expression of CAPRIN-1. Particularly after surgery, more effective prevention of cancer recurrences or prolonged survival period can be obtained against cancer with the expression of CAPRIN-1, which has been conventionally treated with an antitumor agent alone.
  • antitumor agent to be administered in combination with the antibody of the present invention examples include the following known antitumor agents as in prior art literatures or the like, such as paclitaxel, doxorubicin, daunorubicin, cyclophosphamide, methotrexate, 5-fluorouracil, thiotepa, busulfan, improsulfan, piposulfan, benzodopa, carboquone, meturedopa, uredopa, altretamine, triethylenemelamine, triethylenephosphoramide, triethilenethiophosphoramide, trimethylolomelamine, bullatacin, bullatacinone, camptothecin, bryostatin, callystatin, cryptophycin1, cryptophycin8, dolastatin, duocarmycin, eleutherobin, pancratistatin, sarcodictyin, spongistatin, chlorambucil, chlornaphazine,
  • a known radio isotope as in prior art literatures or the like such as 211 At, 131 I, 125 I, 90 Y, 186 Re, 188 Re, 153 Sm, 212 Bi, 32 P, 175 Lu, or 176 Lu can be bound to the antibody of the present invention.
  • a desired radio isotope is effective for treatment or diagnosis of tumor.
  • the antibody of the present invention is an antibody having immunological reactivity with CAPRIN-1, an antibody specifically recognizing CAPRIN-1, or an antibody specifically binding to CAPRIN-1, which exhibits cytotoxic activity against cancer or the effect of suppressing tumor growth.
  • the antibody should have a structure such that rejection is almost or completely avoided in a subject animal to which the antibody is administered. Examples of such an antibody include, when a subject animal is a human, a human antibody, a humanized antibody, a chimeric antibody (e.g., a human-mouse chimeric antibody), a single chain antibody, and a bispecific antibody.
  • These antibodies are: recombinant antibodies having heavy chain and light chain variable regions from a human antibody; recombinant antibodies having heavy chain and light chain variable regions composed of complementarity determining regions (CDRs) (CDR1, CDR2, and CDR3) from a non-human animal antibody and framework regions from a human antibody; or recombinant antibodies having heavy chain and light chain variable regions from a non-human animal antibody; said recombinant antibodies also having heavy chain and light chain constant regions from a human antibody.
  • CDRs complementarity determining regions
  • Preferable antibodies are the former two antibodies.
  • These recombinant antibodies can be prepared as follows by cloning DNA encoding an anti-human CAPRIN-1 monoclonal antibody (e.g., a human monoclonal antibody, a mouse monoclonal antibody, a rat monoclonal antibody, a rabbit monoclonal antibody, or a chicken monoclonal antibody) from an antibody-producing cell such as a hybridoma, preparing DNA encoding a light chain variable region and a heavy chain variable region of the antibody by an RT-PCR method using it as a template, and then determining the sequence of each variable region of light chain and heavy chain or each sequence of CDR1, CDR2, and CDR3 based on a Kabat EU numbering system ( Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institute of Health, Bethesda, Md. (1991 )).
  • an anti-human CAPRIN-1 monoclonal antibody e.g., a human monoclonal
  • DNA encoding each of these variable regions or DNA encoding each CDR is prepared using gene recombination techniques ( Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989 )) or a DNA synthesizer.
  • the above human monoclonal antibody-producing hybridoma can be prepared by immunizing a human antibody-producing animal (e.g., a mouse) with human CAPRIN-1 and then fusing splenocytes excised from the immunized animal to myeloma cells.
  • DNAs encoding a light chain or heavy chain variable region and a constant region from a human antibody are prepared as necessary using gene recombination techniques or a DNA synthesizer.
  • DNA is prepared by substituting a CDR coding sequence in DNA encoding a variable region of light chain or heavy chain derived from a human antibody, with a CDR coding sequence corresponding thereto of an antibody derived from a non-human animal (e.g., a mouse, a rat, or a chicken) and then ligating the DNA thus obtained to DNA encoding a constant region of light chain or heavy chain derived from a human antibody.
  • a non-human animal e.g., a mouse, a rat, or a chicken
  • DNA encoding a chimeric antibody can be prepared by ligating DNA encoding a light chain or heavy chain variable region of an antibody from a non-human animal (e.g., a mouse, a rat, and a chicken) to DNA encoding a light chain or heavy chain constant region from a human antibody.
  • a non-human animal e.g., a mouse, a rat, and a chicken
  • this antibody is an antibody prepared by linearly ligating a heavy chain variable region to a light chain variable region via a linker.
  • DNA encoding a single chain antibody can be prepared by binding DNA encoding a heavy chain variable region, DNA encoding a linker, and DNA encoding a light chain variable region.
  • a heavy chain variable region and a light chain variable region are both from a human antibody, or, only CDRs are substituted with CDRs of an antibody from a non-human animal (e.g., a mouse, a rat, and a chicken) although the other regions are from a human antibody.
  • a linker comprises 12 to 19 amino acids, such as (G 4 S) 3 of 15 amino acids ( G. -B. Kim et al., Protein Engineering Design and Selection 2007, 20 (9): 425-432 ).
  • DNA encoding a bispecific antibody can be prepared by linking DNA encoding a heavy chain variable region A, DNA encoding a light chain variable region B, DNA encoding a heavy chain variable region B, and DNA encoding a light chain variable region A in this order (here, DNA encoding a light chain variable region B is bound to DNA encoding a heavy chain variable region B via DNA encoding the above linker).
  • a heavy chain variable region and a light chain variable region are both from a human antibody, or, a human antibody in which only CDRs have been substituted with CDRs of an antibody from a non-human animal (e.g., a mouse, a rat, or a chicken).
  • the above-prepared recombinant DNA is incorporated into one or a plurality of appropriate vectors, they are introduced into host cells (e.g., mammalian cells, yeast cells, or insect cells), and then (co)expression is caused, so that a recombinant antibody can be prepared ( P. J. Delves., ANTIBODY PRODUCTION ESSENTIAL TECHNIQUES., 1997 WILEY , P. Shepherd and C. Dean., Monoclonal Antibodies., 2000 OXFORD UNIVERSITY PRESS ; J. W. Goding., Monoclonal Antibodies: principles and practice., 1993 ACADEMIC PRESS ).
  • host cells e.g., mammalian cells, yeast cells, or insect cells
  • Examples of the antibody of the present invention prepared by the above method include the following antibody (a), (b), or (c) obtained in Examples below:
  • amino acid sequences represented by SEQ ID NOS: 40, 41, and 42, SEQ ID NOS: 44, 45, and 46, and SEQ ID NO: 60, 61, and 62 are CDR1, CDR2, and CDR3, respectively, of mouse antibody heavy chain variable regions.
  • amino acid sequences represented by SEQ ID NOS: 48, 49, and 50, and SEQ ID NOS: 64, 65, and 66 are CDR1, CDR2, and CDR3, respectively, of mouse antibody light chain variable regions.
  • the humanized antibody, the chimeric antibody, the single chain antibody, or the bispecific antibody of the present invention is the following antibody (exemplified as "antibody (a)"), for example:
  • sequences of human antibody heavy chain and light chain constant regions and variable regions can be obtained from NCBI (e.g., U.S.A.: GenBank, UniGene), for example.
  • NCBI e.g., U.S.A.: GenBank, UniGene
  • the sequence of Accession No. J00228 can be referred to for a human IgG1 heavy chain constant region
  • sequence of Accession No. J00230 can be referred to for a human IgG2 heavy chain constant region
  • sequence of Accession No. X03604 can be referred to for a human IgG3 heavy chain constant region
  • sequence of Accession No. K01316 can be referred to for a human IgG4 heavy chain constant region
  • the sequences of Accession Nos can be obtained from NCBI (e.g., U.S.A.: GenBank, UniGene), for example.
  • the sequence of Accession No. J00228 can be referred to for a human IgG1 heavy chain constant region
  • V00557, X64135, X64133, and the like can be referred to for human light chain ⁇ constant regions, and the sequences of Accession Nos. X64132, X64134, and the like can be referred to for human light chain ⁇ constant regions.
  • the above antibodies preferably have cytotoxic activity and thus can exhibit anti-tumor effects.
  • a hybridoma capable of producing another human antibody or non-human animal antibody (e.g., a mouse antibody) against human CAPRIN-1 is prepared, a monoclonal antibody that is produced by the hybridoma is collected, and then whether or not it is a target antibody is determined by immunological binding property with human CAPRIN-1 and cytotoxic activity as indicators.
  • DNA encoding heavy chain and light chain variable regions of the target antibody is prepared from the hybridoma as described above, sequencing is carried out, and then the DNA is used for preparation of another antibody.
  • each of the above antibodies (a) to (c), and particularly the sequence of the framework region and/or the sequence of the constant region of each of the antibodies may have a substitution, a deletion, or an addition of one or several amino acids, as long as it has specificity for specific recognition of CAPRIN-1.
  • severe refers to preferably 2 to 5, and more preferably 2 or 3.
  • the present invention further provides DNA encoding the above antibody of the present invention, or, DNA encoding the above antibody heavy chain or light chain, or, DNA encoding the above antibody heavy chain or light chain variable region.
  • DNA include, in the case of antibody (a), DNA encoding a heavy chain variable region comprising the nucleotide sequences encoding the amino acid sequences of SEQ ID NOS: 40, 41, and 42 and DNA encoding a light chain variable region comprising the nucleotide sequences encoding the amino acid sequences of SEQ ID NOS: 48, 49, and 50.
  • Complementarity determining regions (CDRs) encoded by the sequences of DNA are regions for determining the specificity of an antibody.
  • sequences encoding regions in an antibody other than CDRs may be from other antibodies.
  • other antibodies include antibodies from non-human organisms, and are preferably from a human in view of reduction of side effects.
  • regions encoding each framework region and each contact region of heavy chains and light chains preferably comprise nucleotide sequences encoding corresponding amino acid sequences from a human antibody.
  • DNA encoding the antibody of the present invention include, in the case of antibody (a), DNA encoding a heavy chain variable region comprising the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 43 and DNA encoding a light chain variable region comprising the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 51.
  • an example of the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 43 is the nucleotide sequence of SEQ ID NO: 52.
  • an example of the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 51 is the nucleotide sequence of SEQ ID NO: 53.
  • regions encoding each constant region of heavy chains and light chains preferably comprise nucleotide sequences encoding the corresponding amino acid sequences from a human antibody.
  • the DNAs of these antibodies can be obtained by the above methods or the following method, for example.
  • total RNA is prepared from a hybridoma relating to the antibody of the present invention using a commercially available RNA extraction kit, and then cDNA is synthesized with reverse transcriptase using random primers, and the like.
  • cDNA encoding an antibody is amplified by a PCR method using as primers the oligonucleotides of sequences conserved in each variable region of known mouse antibody heavy chain and light chain genes.
  • the sequence encoding a constant region can be obtained by amplifying a known sequence by a PCR method.
  • the nucleotide sequence of DNA can be determined by a conventional method such as insertion of it into a plasmid or a phage for sequencing.
  • An anti-CAPRIN-1 antibody to be used in the present invention is considered to exhibit the anti-tumor effects against CAPRIN-1-expressing cancer cells through the following mechanism:
  • ADCC effector-cell antibody-dependent cytotoxicity
  • CDC complement-dependent cytotoxicity
  • an anti-CAPRIN-1 antibody to be used in the present invention can be evaluated by, as specifically described in Examples below, measuring ex vivo the above ADCC activity or CDC activity against CAPRIN-1-expressing cancer cells.
  • an anti-CAPRIN-1 antibody to be used in the present invention binds to a CAPRIN-1 protein on a cancer cell and exhibits anti-tumor effects due to the above activity, and thus it is useful for treating or preventing cancer.
  • the present invention provides a pharmaceutical composition for treating and/or preventing a cancer, which comprises an anti-CAPRIN-1 antibody as an active ingredient.
  • the anti-CAPRIN-1 antibody is used for administration thereof to a human body (antibody therapy), it is preferably human antibody or humanized antibody in order to decrease immunogenicity.
  • binding constant (affinity constant) Ka (k on /k off ) is preferably at least 10 7 M -1 , at least 10 8 M -1 , at least 5 x 10 8 M -1 , at least 10 9 M -1 at least 5 x 10 9 M -1 , at least 10 10 M -1 , at least 5 x 10 10 M -1 at least 10 11 M -1 , at least 5 x 10 11 M -1 , at least 10 12 M -1 , or, at least 10 13 M -1 .
  • the capacity of an antibody to bind to CAPRIN-1 can be specified by binding assay using ELISA, a Western blot method, immuno-fluorescence and flow cytometric analysis, or the like as described in Examples.
  • An antibody that recognizes CAPRIN-1 can be tested for reactivity to CAPRIN-1 by a method for immunohistochemistry known by persons skilled in the art using paraformaldehyde- or acetone-fixed frozen sections or paraformaldehyde-fixed paraffin-embedded tissue sections, which is prepared from tissue samples obtained from a patient during surgery, or tissue samples obtained from an animal having heterotransplant tissue inoculated with a cell line expressing CAPRIN-1, naturally or after transfection.
  • An antibody reactive to CAPRIN-1 can be stained by various methods for immunohistochemical staining. For example, a horseradish peroxidase-conjugated goat anti-mouse antibody or goat anti-chicken antibody is caused to undergo reaction, a target antibody can be visualized.
  • a target of the pharmaceutical composition for treating and/or preventing a cancer of the present invention is not particularly limited, as long as it is cancer (cell) expressing a CAPRIN-1 gene.
  • tumor and cancer as used herein refers to malignant neoplasm and is used interchangeably.
  • Cancer to be subjected to the present invention is cancer expressing genes encoding CAPRIN-1 proteins having amino acid sequences of even-numbered SEQ ID NOS: 2 to 30.
  • cancer include preferably breast cancer, brain tumor, leukemia, lung cancer, lymphoma, mastocytoma, renal cancer, uterine cervix cancer, bladder cancer, esophageal cancer, gastric cancer, colorectal cancer, ovarian cancer, prostate cancer, and fibrosarcoma.
  • Examples of such specific cancer include, but are not limited to, breast adenocarcinoma, composite type breast adenocarcinoma, mammary gland malignant mixed tumor, intraductal papillary adenocarcinoma, lung adenocarcinoma, squamous cell carcinoma, small cell carcinoma, large cell carcinoma, glioma that is neural epithelial tissue tumor, ependymoma, neurocytoma, fetal neuroectodermal tumor, schwannoma, neurofibroma, meningioma, chronic lymphocytic leukemia, lymphoma, gastrointestinal lymphoma, digestive lymphoma, small-cell to medium-cell lymphoma, cancer of cecum, ascending colon cancer, descending colon cancer, transverse colon cancer, sigmoid colon cancer, rectal cancer, epithelial ovarian cancer, germ cell tumor, and interstitial cell tumor.
  • preferable subjects are mammals including primates, pet animals, domestic animals, animals for race, and the like and are particularly preferably humans, dogs, and cats.
  • an antibody to be used in the present invention when used as a pharmaceutical composition, it can be formulated by a method known by persons skilled in the art.
  • the antibody can be used parenterally in the form of an injection preparation such as an aseptic solution or a suspension prepared with water or a pharmacologically acceptable solution other than water.
  • it can be formulated by mixing in a unit dosage form required by generally accepted pharmaceutical practice in appropriate combination with a pharmacologically acceptable carrier or medium, specifically, sterile water or saline, vegetable oil, an emulsifier, a suspension, a surfactant, a stabilizer, a flavoring compound, an excipient, a vehicle, an antiseptic, a binder, and the like.
  • the amounts of active ingredients in these preparations are determined so that an appropriate dose within the indicated range can be obtained.
  • An aseptic composition for injection can be prescribed according to general pharmaceutical practice using a vehicle such as distilled water for injection.
  • an aqueous solution for injection examples include saline, an isotonic solution containing dextrose or other adjuvants, such as D-sorbitol, D-mannose, D-mannitol, and sodium chloride. These examples may be used in combination with an appropriate solubilizing agent such as alcohol, specifically ethanol and polyalcohol (e.g., propylene glycol and polyethylene glycol), and nonionic surfactant (e.g., polysorbate 80 (TM) and HCO-60).
  • an appropriate solubilizing agent such as alcohol, specifically ethanol and polyalcohol (e.g., propylene glycol and polyethylene glycol), and nonionic surfactant (e.g., polysorbate 80 (TM) and HCO-60).
  • oils examples include sesame oil and soybean oil, which can be used in combination with a solubilizing agent such as benzyl benzoate or benzyl alcohol.
  • a buffering agent such as phosphate buffer or sodium acetate buffer
  • a soothing agent such as procaine hydrochloride
  • a stabilizer such as benzyl alcohol or phenol
  • an antioxidant may be combined therewith.
  • An appropriate amplus is generally filled with the thus prepared injection solution.
  • Administration is peroral or perenteral administration and is preferably perenteral administration.
  • Specific examples of the route of administration include injection, transnasal administration, pulmonary administration, and transdermal administration.
  • Examples of injection include intravenous injection, intramuscular injection, intraperitoneal injection, and subcutaneous injection, so that systemic or local administration is possible.
  • administration methods can be appropriately selected depending on a patient's age, body weight, gender, symptoms, and the like.
  • the dosage per administration of a pharmaceutical composition containing an antibody or a polynucleotide encoding the antibody can be selected from the range between 0.0001 mg and 1000 mg per kg of body weight, for example.
  • dosage can be selected from the range between 0.001 mg/body and 100000 mg/body per patient.
  • the dosage range is not always limited to these numerical values.
  • the dosage and administration method are varied depending on a patient's body weight, age, gender, symptoms, and the like, but can be appropriately selected by persons skilled in the art.
  • the above pharmaceutical composition containing the antibody or a fragment thereof of the present invention is administered to a subject, so that cancer, preferably, breast cancer, brain tumor, leukemia, lung cancer, lymphoma, mastocytoma, renal cancer, uterine cervix cancer, bladder cancer, esophageal cancer, gastric cancer, and colorectal cancer can be treated and/or prevented.
  • cancer preferably, breast cancer, brain tumor, leukemia, lung cancer, lymphoma, mastocytoma, renal cancer, uterine cervix cancer, bladder cancer, esophageal cancer, gastric cancer, and colorectal cancer
  • the present invention further encompasses a method for treating and/or preventing a cancer, comprising administering to a subject the pharmaceutical composition of the present invention in combination with the above exemplified antitumor agent or pharmaceutical composition containing such antitumor agent.
  • the antibody or a fragment thereof of the present invention and an antitumor agent may be administered simultaneously or separately to a subject. They can be separately administered regardless of the order of administration.
  • the administration intervals, dosage, the route of administration, and the frequency of administration can be appropriately selected by a specialist.
  • Examples of the other pharmaceutical formulation to be administered simultaneously include pharmaceutical compositions obtained by mixing the antibody or a fragment thereof of the present invention with an antitumor agent in a pharmacologically acceptable carrier (or a medium) followed by formulation.
  • explanations concerning prescription, formulation, the route of administration, dose, cancer, and the like for administration of a pharmaceutical composition containing the antibody of the present invention and formulation are applicable.
  • the present invention also provides a pharmaceutical combination for treating and/or preventing a cancer, comprising the pharmaceutical composition of the present invention, and the above exemplified pharmaceutical composition containing an antitumor agent. Also, the present invention provides a pharmaceutical composition for treating and/or preventing a cancer, comprising the antibody or a fragment thereof of the present invention and an antitumor agent together with a pharmacologically acceptable carrier.
  • the present invention further provides the following polypeptides and DNAs relating to the above antibody (a), (b), or (c).
  • polypeptides and DNAs can be prepared using gene recombination techniques as described above.
  • RNA was extracted from a testis tissue of a healthy dog by an acid guanidium-phenol-chloroform method.
  • PolyA RNA was purified according to protocols included with an Oligotex-dT30 mRNA purification Kit (Takara Shuzo Co., Ltd.) using the kit.
  • a dog testis cDNA phage library was synthesized using the thus obtained mRNA (5 ⁇ g).
  • a cDNA synthesis kit, a ZAP-cDNA synthesis kit, and a ZAP-cDNA gigapack III gold cloning kit (STRATAGENE) were used and the library was prepared according to protocols included with the kit.
  • the size of the thus prepared cDNA phage library was 7.73 x 10 5 pfu/ml.
  • Immunoscreening was carried out using the above-prepared dog testis cDNA phage library. Specifically, host Escherichia coli (XL1-Blue MRF') was infected with the phage so that 2210 clones were present on a ⁇ 90 x 15 mm NZY agarose plate. Cells were cultured at 42°C for 3 to 4 hours, so as to cause plaque formation. The plate was covered with a nitrocellulose membrane (Hybond C Extra: GE HealthCare Bio-Sciences) impregnated with IPTG (isopropyl- ⁇ -D-thiogalactoside) at 37°C for 4 hours. Proteins were induced, expressed, and then transferred to the membrane.
  • Hoscherichia coli XL1-Blue MRF'
  • IPTG isopropyl- ⁇ -D-thiogalactoside
  • the membrane was recovered, immersed, and shaken in TBS (10 mM Tris-HCl, 150 mM NaCl pH 7.5) containing 0.5% powdered skim milk at 4°C overnight, so that nonspecific reaction was suppressed.
  • TBS 10 mM Tris-HCl, 150 mM NaCl pH 7.5
  • the filter was caused to react with 500-fold diluted sera of dogs with cancer at room temperature for 2 to 3 hours.
  • the collected Escherichia coli /phage extract was passed through a NHS-column (GE HealthCare Bio-Sciences), so as to immobilize the Escherichia coli ⁇ phage-derived protein.
  • the serum of a dog with cancer was passed through the column to which the protein had been immobilized for reaction, thereby removing Escherichia coli and antibodies adsorbed to the phage from the serum.
  • Each serum fraction that had passed through the column was diluted 500-fold with TBS containing 0.5% powdered skim milk, and the resultant was used as an immunoscreening material.
  • Colonies corresponding to the color reaction positive site were collected from the ⁇ 90 x 15 mm NZY agarose plate, and then dissolved in 500 ⁇ l of SM buffer (100 mM NaCl, 10 mM MgClSO 4 , 50 mM Tris-HCl, 0.01% gelatin, pH 7.5). Until unification of color reaction positive colonies, secondary screening and tertiary screening were repeated by a method similar to the above. Thus, 30940 phage clones that had reacted with serum IgG were screened so that 5 positive clones were isolated.
  • SM buffer 100 mM NaCl, 10 mM MgClSO 4 , 50 mM Tris-HCl, 0.01% gelatin, pH 7.5.
  • a procedure for conversion of phage vectors to plasmid vectors was performed for the 5 positive clones isolated by the above method for the purpose of subjecting the clones to nucleotide sequence analysis. Specifically, 200 ⁇ l of a solution of host Escherichia coli (XL1-Blue MRF') prepared to give an absorbance OD 600 of 1.0, 250 ⁇ l of a purified phage solution, and 1 ⁇ l of ExAssist helper phage (STRATAGENE) were mixed and allowed to react at 37°C for 15 minutes.
  • the purified plasmid was subjected to the analysis of the entire sequence of the insert by the primer walking method using the T3 primer of SEQ ID NO: 31 and the T7 primer of SEQ ID NO: 32.
  • the gene sequences of SEQ ID NOS: 5, 7, 9, 11, and 13 were obtained by the sequence analysis.
  • homology search program BLAST search http://www.ncbi.nlm.nih.gov/BLAST/
  • BLAST search http://www.ncbi.nlm.nih.gov/BLAST/
  • sequence identities among the five genes were 100% at the nucleotide sequence level and 99% at the amino acid sequence level in the regions to be translated into proteins.
  • sequence identities of these genes and the human homologue-encoding gene were 94% at the nucleotide sequence level and 98% at the amino acid sequence level in the regions to be translated into proteins.
  • the nucleotide sequences of the human homologues are represented by SEQ ID NOS: 1 and 3 and the amino acid sequences of the same are represented by SEQ ID NOS: 2 and 4.
  • sequence identities of the obtained dog genes and the cattle homologue-encoding gene were 94% at the nucleotide sequence level and 97% at the amino acid sequence level in the regions to be translated into proteins.
  • the nucleotide sequence of the cattle homologue is represented by SEQ ID NO: 15 and the amino acid sequence of the same is represented by SEQ ID NO: 16.
  • sequence identities of the human homologue-encoding genes and the cattle homologue-encoding gene were 94% at the nucleotide sequence level and 93% to 97% at the amino acid sequence level in the regions to be translated into proteins.
  • sequence identities of the obtained dog genes and the horse homologue-encoding gene were 93% at the nucleotide sequence level and 97% at the amino acid sequence level in the regions to be translated into proteins.
  • the nucleotide sequence of the horse homologue is represented by SEQ ID NO: 17 and the amino acid sequence of the same is represented by SEQ ID NO: 18.
  • sequence identities of the human homologue-encoding genes and the horse homologue-encoding gene were 93% at the nucleotide sequence level and 96% at the amino acid sequence level in the regions to be translated into proteins.
  • sequence identities of the obtained dog genes and the mouse homologue-encoding genes were 87% to 89% at the nucleotide sequence level and 95% to 97% at the amino acid sequence level in the regions to be translated into proteins.
  • the nucleotide sequences of the mouse homologues are represented by SEQ ID NOS: 19, 21, 23, 25, and 27 and the amino acid sequences of the same are represented by SEQ ID NOS: 20, 22, 24, 26, and 28.
  • sequence identities of the human homologue-encoding genes and the mouse homologue-encoding genes were 89% to 91% at the nucleotide sequence level and were 95% to 96% at the amino acid sequence level in the regions to be translated into proteins.
  • sequence identities of the obtained dog genes and the chicken homologue-encoding gene were 82% at the nucleotide sequence level and 87% at the amino acid sequence level in the regions to be translated into proteins.
  • the nucleotide sequence of the chicken homologue is represented by SEQ ID NO: 29 and the amino acid sequence of the same is represented by SEQ ID NO: 30.
  • sequence identities of the human homologue-encoding genes and the chicken homologue-encoding gene were 81% to 82% at the nucleotide sequence level and 86% at the amino acid sequence level in the regions to be translated into proteins.
  • PCR was performed as follows using primers of SEQ ID NOS: 33 and 34 specific to the obtained genes.
  • reagents and an accompanying buffer were added to 0.25 ⁇ l of the sample prepared by the reverse transcription reaction to a total volume of 25 ⁇ l, so that the resultant contained the above primers of 2 ⁇ M each, dNTPs of 0.2 mM each, and 0.65 U ExTaq polymerase (Takara Shuzo Co., Ltd.).
  • PCR was carried out by repeating a cycle of 94°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds 30 times using a Thermal Cycler (BIO RAD).
  • the above gene-specific primers are capable of amplifying the region ranging from nucleotides 206 to 632 in the nucleotide sequence of SEQ ID NO: 5 (dog CAPRIN-1 gene) and the region ranging from nucleotides 698 to 1124 in the nucleotide sequence of SEQ ID NO: 1 (human CAPRIN-1 gene).
  • GAPDH-specific primers of SEQ ID NOS: 35 and 36 were also used concurrently.
  • Fig. 1 strong expression was observed in testis among normal dog tissues, while expression was observed in dog breast cancer and adenocarcinoma tissues.
  • the observation of the expression of the human homologues from the obtained genes was also carried out.
  • CAPRIN-1 As a result, similarly to the case of the dog CAPRIN-1 gene, expression could be observed in only testis among normal tissues. However, in the case of cancer cells, expression was detected in many types of cancer cell lines, including breast cancer, brain tumor, leukemia, lung cancer, and esophageal cancer cell lines. Expression was observed particularly in many breast cancer cell lines. It was confirmed by the results that the expression of CAPRIN-1 is not observed in normal tissues other than testis, while CAPRIN-1 was expressed in many cancer cells and particularly in breast cancer cell lines.
  • reference number 1 on each vertical axis indicates the expression patterns of genes identified above and reference number 2 indicates the expression patterns of the GAPDH gene as a control.
  • CAPRIN-1-derived peptide represented by SEQ ID NO: 37 was synthesized. 1 mg of the peptide as an antigen was mixed with an equivalent amount of an incomplete Freund's adjuvant (IFA) solution. The mixture was subcutaneously administered to rabbits 4 times every 2 weeks. Blood was then collected and antiserum containing polyclonal antibodies was obtained. Furthermore, the antiserum was purified using a protein G carrier (GE HealthCare Bio-Sciences), so that polyclonal antibodies against the CAPRIN-1-derived peptide were obtained. Also, the serum from a rabbit to which no antigen had been administered was purified using a protein G carrier in a manner similar to the above, and the resultant was used as a control antibody.
  • IFA incomplete Freund's adjuvant
  • Percentage of increase in mean fluorescence intensity ((MFI level in cells having reacted with anti-human CAPRIN-1 antibody) - (MFI level of control)) / (MFI level of control) x 100.
  • CAPRIN-1 expression was analyzed for 3 renal cancer cell lines (Caki-1, Caki-2, and A498), ovarian cancer cell line (SKOV3), lung cancer cell line (QG56), prostate cancer cell line (PC3), uterine cervix cancer cell line (Hela), fibrosarcoma cell line (HT1080), 2 brain tumor cell lines (T98G and U87MG), 2 mouse colorectal cancer cell lines (CT26 and colon 26), mouse breast cancer cell line (4T1), mouse melanoma cell line (B16), and 2 mouse neuroblastoma cell lines (N1E-115 and Neuro2a).
  • CAPRIN-1 expression was confirmed in all cell lines.
  • mice (Balb/c, female) and dogs (beagles, female) were exsanguinated under ether anesthesia and ketamine/isoflurane anesthesia.
  • each organ stomach, liver, eyeball, thymus gland, muscle, bone marrow, uterus, small bowel, esophagus, heart, kidney, salivary gland, large bowel, mammary gland, brain, lung, skin, adrenal gland, ovary, pancreas, spleen, and bladder
  • Each organ was cut open in PBS and then subjected to perfusion fixation overnight in 0.1 M phosphate buffer (pH 7.4) containing 4% paraformaldehyde (PFA).
  • the perfusion solution was discarded, the tissue surface of each organ was rinsed with PBS, a PBS solution containing 10% sucrose was added to a 50-ml centrifuge tube, each tissue was added to the tube, and then the tube was shaken using a rotor at 4°C for 2 hours.
  • the solution was replaced by a PBS solution containing 20% sucrose, and then left to stand at 4°C until the tissue sank.
  • the solution was replaced by a PBS solution containing 30% sucrose and then left to stand at 4°C until the tissue sank.
  • tissue was removed and then needed portions were excised with a surgical scalpel.
  • an OCT compound Tissue Tek was added to the tissue so that it was thoroughly applied to the tissue surface, and then the tissue was placed in a cryomold.
  • the cryomold was placed on dry ice for quick freezing. Thereafter, the tissue was sliced to 10 ⁇ m to 20 ⁇ m using a cryostat (LEICA). Slices were air-dried on slide glasses using a hair dryer for 30 minutes, to prepare the sliced tissue mounted on a slide glass.
  • each sample was placed in a staining bottle filled with PBS-T (saline containing 0.05% Tween20) and then subjected to replacement with PBS-T being repeated three times every 5 minutes.
  • PBS-T saline containing 0.05% Tween20
  • DAKOPEN DAKOPEN
  • MOM mouse Ig blocking reagent VECTASTAIN
  • PBS-T solution containing 10% FBS were overlaid on mouse tissue and dog tissue, respectively, and then left to stand in a moist chamber at room temperature for 1 hour.
  • a solution of the polyclonal antibodies (reactive with the surfaces of cancer cells, and prepared in (5) above) against the CAPRIN-1-derived peptide (SEQ ID NO: 37) of 10 ⁇ g/ml adjusted with a blocking solution was placed on and then left to stand overnight in a moist chamber at 4°C.
  • a DAB coloring solution (DAB 10 mg + 30% H 2 O 2 10 ⁇ l/0.05 M Tris-HCl (pH 7.6) 50 ml) was placed on, and then the sample was left to stand in a moist chamber at room temperature for 30 minutes.
  • a hematoxylin reagent (DAKO) was placed on, the sample was left to stand at room temperature for 1 minute, and then rinsed with distilled water.
  • the slide glass was immersed in 70%, 80%, 90%, 95%, and then 100% ethanol solutions in such order for 1 minute each and then left to stand overnight in xylene.
  • the slide glass was removed, sealed in Glycergel Mounting Medium (DAKO), and then observed.
  • DAKO Glycergel Mounting Medium
  • Frozen section slides were prepared by a method similar to the above using 108 frozen breast cancer tissue specimens of dogs pathologically diagnosed as having malignant breast cancer, and immunohistochemical staining was performed using the polyclonal antibodies (prepared in (5) above) against the CAPRIN-1-derived peptide (SEQ ID NO: 37).
  • the expression of CAPRIN-1 was observed in 100 out of 108 specimens (92.5%) and CAPRIN-1 was strongly expressed on the surfaces of cancer cells with a particularly high grade of atypism.
  • similar results were obtained in the case of using the monoclonal antibody #1, #2, or #3 obtained in Example 3.
  • Immunohistochemical staining was performed using 188 breast cancer tissue specimens on a paraffin-embedded human breast cancer tissue array (BIOMAX). After 3 hours of treatment of the human breast cancer tissue array at 60°C, the array was placed in a staining bottle filled with xylene, followed by xylene replacement being repeated three times every 5 minutes. Next, a similar procedure was performed with ethanol and PBS-T instead of xylene. The human breast cancer tissue array was placed in a staining bottle filled with 10 mM citrate buffer (pH 6.0) containing 0.05% Tween20. After 5 minutes of treatment at 125°C, the array was left to stand at room temperature for 40 minutes or more.
  • BIOMAX paraffin-embedded human breast cancer tissue array
  • Immunohistochemical staining was performed according to a method similar to that used in (7)-3 above with 247 malignant brain tumor tissue specimens on a paraffin-embedded human malignant brain tumor tissue array (BIOMAX), using the polyclonal antibodies (prepared in (5) above) against the CAPRIN-1-derived peptide (SEQ ID NO: 37).
  • BIOMAX paraffin-embedded human malignant brain tumor tissue array
  • the polyclonal antibodies prepared in (5) above
  • SEQ ID NO: 37 the CAPRIN-1-derived peptide
  • Immunohistochemical staining was performed according to a method similar to that in (7)-3 above with 150 breast cancer metastasized lymph node tissue specimens on a paraffin-embedded human breast cancer metastasized lymph node tissue array (BIOMAX), using the polyclonal antibodies against the CAPRIN-1-derived peptide (SEQ ID NO: 37) prepared in (5) above.
  • BIOMAX paraffin-embedded human breast cancer metastasized lymph node tissue array
  • SEQ ID NO: 37 CAPRIN-1-derived peptide
  • a recombinant protein from the human homologous gene was prepared by the following method. PCR was performed in a total volume of 50 ⁇ l with 1 ⁇ l of cDNA, two primers (SEQ ID NOS: 38 and 39 comprising Sac I and Xho I restriction enzyme cleavage sequences) of 0.4 ⁇ M each, 0.2 mM dNTP, and 1.25U PrimeSTAR HS polymerase (Takara Shuzo Co., Ltd.), prepared by adding the reagents and an accompanying buffer. The expression had been confirmed by an RT-PCR method for the cDNA used herein from among various tissue ⁇ or cell-derived cDNAs prepared in Example 1.
  • PCR was preformed by repeating a cycle of 98°C for 10 seconds and 68°C for 2.5 minutes 30 times using a Thermal Cycler (BIO RAD).
  • the above two primers are capable of amplifying a region encoding the entire amino acid sequence of SEQ ID NO: 2.
  • the thus amplified DNA was subjected to electrophoresis on 1% agarose gel, and then an about 2.1 kbp DNA fragment was purified using a QIAquick Gel Extraction Kit (QIAGEN).
  • the thus purified DNA fragment was ligated to a cloning vector PCR-Blunt (Invitrogen). After transformation of Escherichia coli with it, plasmid was collected. It was verified by sequencing that the thus amplified gene fragment has the sequence of interest.
  • the plasmid having a matched sequence with the sequence of interest was treated with Sac I and Xho I restriction enzymes and then purified with a QIAquick Gel Extraction Kit.
  • the gene sequence of interest was inserted into an Escherichia coli expression vector pET30a (Novagen) treated with Sac I and Xho I restriction enzymes. A His-tag fused recombinant protein can be produced using the vector.
  • the plasmid was transformed into Escherichia coli for recombinant expression, BL21(DE3), and then expression was induced with 1 mM IPTG, so that the protein of interest was expressed in Escherichia coli.
  • the above-obtained recombinant Escherichia coli expressing the gene of SEQ ID NO: 1 was cultured in LB medium containing 30 ⁇ g/ml kanamycin at 37°C until absorbance at 600 nm reached around 0.7, isopropyl- ⁇ -D-1-thiogalactopyranoside was added at a final concentration of 1 mM, and then cells were cultured at 37°C for 4 hours. Subsequently, centrifugation was performed at 4800 rpm for 10 minutes and then cells were collected. The resulting cell pellet was suspended in phosphate buffered saline and centrifuged at 4800 rpm for 10 minutes, and then cells were washed.
  • the cells were suspended in phosphate buffered saline and then disrupted by ultrasonication on ice.
  • the resulting lysate of the ultrasonicated Escherichia coli was subjected to centrifugation at 6000 rpm for 20 minutes, and then the resulting supernatant was regarded as a soluble fraction and the precipitate was regarded as an insoluble fraction.
  • the soluble fraction was added to a nickel chelate column adjusted according to a conventional method (carrier: Chelating Sepharose TM Fast Flow (GE HealthCare); column capacity of 5 ml; and equilibration buffer: 50 mM hydrochloride buffer (pH 8.0)). Unadsorbed fractions were washed off with 50 mM hydrochloride buffer (pH 8.0) in an amount 10 times the column capacity and 20 mM phosphate buffer (pH 8.0) containing 20 mM imidazole. Immediately after washing, 6 beds were eluted with 20 mM phosphate buffer (pH 8.0) containing 100 mM imidazole.
  • the elution of the protein of interest was confirmed by Coomassie staining on the elution fraction with 20 mM phosphate buffer (pH 8.0) containing 100 mM imidazole, and then the elution fraction was added to a strong anion exchange column (carrier: Q Sepharose TM Fast Flow (GE HealthCare); column capacity of 5 ml; and 20 mM phosphate buffer (pH 8.0) as an equilibration buffer).
  • An unadsorbed fraction was washed off with 20 mM phosphate buffer (pH 7.0) in an amount 10 times the column capacity and 20 mM phosphate buffer (pH 7.0) containing 200 mM sodium chloride.
  • 5 beds were eluted with 20 mM phosphate buffer (pH 7.0) containing 400 mM sodium chloride, and thus the purified fraction of the protein having the amino acid sequence represented by SEQ ID NO: 2 was obtained.
  • Example 2 100 ⁇ g of the antigen protein (human CAPRIN-1) represented by SEQ ID NO: 2 prepared in Example 2 was mixed with an equivalent amount of MPL+TDM adjuvant (Sigma), and then this was used as an antigen solution per one mouse.
  • the antigen solution was intraperitoneally administered to 6-week-old Balb/cc mice (Japan SLC Inc.), and then the administration was performed 7 times every week, and thus immunization was completed. Each spleen was excised 3 days after the final immunization, and sandwiched between two sterilized slide glasses and then crushed. The resultant was washed with PBS(-) (Nissui) and then centrifuged at 1500 rpm for 10 minutes to remove the supernatant.
  • PBS(-) Nasui
  • splenocytes were obtained.
  • the thus obtained splenocytes and mouse myeloma cells SP2/0 purchased from ATCC
  • a PEG solution prepared by mixing 200 ⁇ l of RPMI1640 medium containing 10% FBS heated at 37°C and 800 ⁇ l of PEG1500 (Boehringer) was added to the mixture, left to stand for 5 minutes for cell fusion, and then subjected to centrifugation at 1700 rpm for 5 minutes.
  • cells were suspended in 150 ml of RPMI1640 medium containing 15% FBS, supplemented with a HAT solution (Gibco) (2% equivalent) (HAT selective medium), and then the cell suspension was plated on fifteen 96-well plates (Nunc) at 100 ⁇ l per well. Cells were cultured for 7 days at 37°C under conditions of 5% CO 2 , so that hybridomas prepared by fusion of splenocytes and myeloma cells were obtained.
  • Hybridomas were selected using as a marker the binding affinity of the antibody produced by the prepared hybridomas to the CAPRIN-1 protein.
  • the CAPRIN-1 protein solution (1 ⁇ g/ml) prepared in Example 2 was added to a 96-well plate at 100 ⁇ l per well and then left to stand at 4°C for 18 hours. Each well was washed 3 times with PBS-T, 400 ⁇ l of a 0.5% Bovine Serum Albumin (BSA) solution (SIGMA) was added per well, and then the plate was left to stand at room temperature for 3 hours. The solution was removed, and then the wells were washed three times with 400 ⁇ l of PBS-T per well.
  • BSA Bovine Serum Albumin
  • the culture supernatant of the above-obtained hybridomas was added at 100 ⁇ l per well, and then left to stand at room temperature for 2 hours. After washing each well three times with PBS-T, an HRP-labeled anti-mouse IgG (H+L) antibody (Invitrogen) diluted 5000-fold with PBS was added at 100 ⁇ l per well and the resultant was then left to stand at room temperature for 1 hour. After washing the wells three times with PBS-T, 100 ⁇ l of a TMB substrate solution (Thermo) was added per well and then left to stand for 15 to 30 minutes for coloring reaction.
  • HRP-labeled anti-mouse IgG (H+L) antibody Invitrogen
  • hybridomas were added to a 96-well plate at 0.5 cells per well and then cultured. After 1 week, hybridomas that had formed single colonies in wells were observed. These cells in the wells were further cultured, and then hybridomas were selected using as a marker the binding affinity of antibodies produced by the cloned hybridomas to the CAPRIN-1 protein.
  • the CAPRIN-1 protein solution (1 ⁇ g/ml) prepared in Example 2 was added to a 96-well plate at 100 ⁇ l per well, and then left to stand at 4°C for 18 hours. Each well was washed with PBS-T three times, 400 ⁇ l of a 0.5% BSA solution was added per well, and then the resultant was left to stand at room temperature for 3 hours.
  • antibodies reactive to the cell surfaces of breast cancer cells expressing CAPRIN-1 were selected. Specifically, 10 6 cells of the human breast cancer cell line MDA-MB-231V were subjected to centrifugation in a 1.5-ml microcentrifuge tube, and 100 ⁇ l of the culture supernatant of each of the above hybridomas was added to the tube, and then the tube was left to stand on ice for 1 hour. After washing with PBS, an FITC-labeled goat anti-mouse IgG antibody (Invitrogen) diluted 500-fold with PBS containing 0.1% FBS was added, and then the resultant was left to stand on ice for 1 hour.
  • FITC-labeled goat anti-mouse IgG antibody Invitrogen
  • mRNA was extracted from each hybridoma cell line producing either one of the three monoclonal antibodies selected in Example 3.
  • An RT-PCR method using primers specific to the mouse FR1-derived sequence and the mouse FR4-derived sequence was performed therefor, and the genes of the heavy chain variable (VH) regions and the genes of the light chain variable (VL) regions of all anti-CAPRIN-1 monoclonal antibodies were obtained.
  • VH heavy chain variable
  • VL light chain variable
  • mRNA was prepared from 10 6 cells of each hybridoma cell line using a mRNA micro purification kit (GE HealthCare). The thus obtained mRNA was reverse-transcribed and then cDNA was synthesized using a SuperScriptII 1st strand Synthesis Kit (Invitrogen). These procedures were performed according to protocols attached to each kit.
  • Antibody gene amplification was performed by a PCR method using the thus obtained cDNA.
  • a primer (SEQ ID NO: 54) specific to the mouse heavy chain FR1 sequence and a primer (SEQ ID NO: 55) specific to the mouse heavy chain FR4 sequence were used.
  • a primer (SEQ ID NO: 56) specific to the mouse light chain FR1 sequence and a primer (SEQ ID NO: 57) specific to the mouse light chain FR4 were used.
  • These primers were designed in reference to Jones, S. T. and Bending, M. M. Bio/Technology 9, 88-89 (1991 ). Ex-taq (Takara Bio Inc.) was used for PCR.
  • a cDNA sample was added to 5 ⁇ l of 10 x EX Taq Buffer, 4 ⁇ l of dNTP Mixture (2.5 mM), primers (1.0 ⁇ M) (2 ⁇ l each), and 0.25 ⁇ l of Ex Taq (5 U/ ⁇ l), and then the total amount thereof was adjusted with sterile water to 50 ⁇ l.
  • PCR was performed under the conditions: 2 minutes of treatment at 94°C, followed by 30 cycles of 1 minute of denaturation at 94°C, 30 seconds of annealing at 58°C, and 1 minute of extension reaction at 72°C.
  • PCR products were each subjected to agarose gel electrophoresis, and DNA bands of the VH region and the VL region were excised.
  • DNA fragments were purified using a QIAquick Gel purification kit (QIAGEN) according to the accompanying protocols.
  • the purified DNA was cloned into a pCR2.1 vector using a TA cloning kit (Invitrogen).
  • the ligated vector was transformed into DH5a competent cells (TOYOBO) according to a conventional method. 10 clones of each transformant were cultured overnight in medium (100 ⁇ g/ml ampicillin) at 37°C, and then plasmid DNA was purified using a Qiaspin Miniprep kit (QIAGEN).
  • the gene sequences of the VH region and the VL region in each plasmid obtained above were analyzed with an M13 forward primer (SEQ ID NO: 58) and an M13 reverse primer (SEQ ID NO: 59) on a fluorescence sequencer (DNA sequencer 3130XL; ABI), using a Big Dye Terminator Ver3.1 Cycle Sequencing Kit (ABI) according to the accompanying protocols. As a result, each gene sequence was determined. The sequences were identical among the 10 clones.
  • the thus obtained gene sequences encoding the monoclonal antibody heavy chain variable regions are shown by SEQ ID NOS: 52, 70, and 68 and the amino acid sequences thereof are shown by SEQ ID NOS: 43, 47, and 51; and the gene sequences encoding the light chain variable regions are shown by SEQ ID NOS: 53 and 69, and the amino acid sequences thereof are shown by SEQ ID NOS: 51 and 67.
  • the monoclonal antibody #1 comprises the heavy chain variable region of SEQ ID NO: 43 and the light chain variable region of SEQ ID NO: 51
  • the monoclonal antibody #2 comprises the heavy chain variable region of SEQ ID NO: 47 and the light chain variable region of SEQ ID NO: 51
  • the monoclonal antibody #3 comprises the heavy chain variable region of SEQ ID NO: 63 and the light chain variable region of SEQ ID NO: 67.
  • 93 candidate peptides each consisting of 12 to 16 amino acids in the amino acid sequence of the human CAPRIN-1 protein, were synthesized, and then each peptide was dissolved in DMSO at a concentration of 1 mg/ml.
  • Each peptide was dissolved in 0.1 M sodium carbonate buffer (pH 9.6) at a concentration of 30 ⁇ g/ml, added to a 96-well plate (Nunc, Product No. 436006) at 100 ⁇ l per well, and then left to stand overnight at 4°C.
  • the solution was discarded, 10 mM ethanolamine/0.1 M sodium carbonate buffer (PH9.6) was added at 200 ⁇ l per well, and then the resultant was left to stand at room temperature for 1 hour.
  • the solution was discarded and then the plate was washed twice with PBS containing 0.5% Tween 20 (PBST), so that a plate onto which each peptide had been immobilized was prepared.
  • PBST PBS containing 0.5% Tween 20
  • the cell culture supernatant containing the mouse monoclonal antibodies (#1, #2, and #3) obtained in Example 3 was added at 50 ⁇ l per well, and then shaken at room temperature for 1 hour. The solution was removed, followed by three times of washing with PBST. Next, a secondary antibody solution prepared by diluting a HRP-labeled anti-mouse IgG (Invitrogen) antibody 3000- to 4000-fold with PBST was added (50 ⁇ l each) to the mouse monoclonal antibodies. The solution was removed, followed by six times of washing with PBST.
  • a TMB substrate solution (Thermo) was added at 100 ⁇ l per well and then left to stand for 15 to 30 minutes for coloring reaction. After color development, 1 N sulfuric acid was added at 100 ⁇ l per well to stop the reaction, and then absorbances at 450 nm and 595 nm were measured using an absorption spectrometer. As a result, a polypeptide comprising the amino acid sequence of SEQ ID NO: 37 was identified as a partial CAPRIN-1 sequence recognized by all of the anti-CAPRIN-1 monoclonal antibodies #1, #2, and #3.
  • polypeptide of SEQ ID NO: 37 contains epitope regions for the anti-CAPRIN-1 antibodies #1, #2, and #3.
  • Example 6 CAPRIN-1 expression on various cancer cell surfaces using anti-CAPRIN-1 antibodies #1, #2, and #3
  • 7 breast cancer cell lines (MDA-MB-157, T47D, MRK-nu-1, MDA-MB-231 V, BT20, SK-BR-3, and DA-MB-231 T) for which CAPRIN-1 gene expression had been observed, and the other 3 breast cancer cell lines (MDA-MB-231C, MCF-7, and ZR75-1), 5 glioma cell lines (T98G, SNB19, U251, U87MG, and U373), 4 renal cancer cell lines (Caki-1, Caki-2, A498, and ACHN), 2 gastric cancer cell lines (MKN28 and MKN45), 5 colorectal cancer cell lines (HT29, LoVo, Caco2, SW480, and HCT116), 3 lung cancer cell lines (A549, QG56, and PC8), 4 leukemia cell lines (AML5, Namalwa, BDCM, RPI1788), one uterine cervix cancer cell line (SW756), one bladder cancer cell line (T24),
  • Percentage of increase in mean fluorescence intensity percentage of enhancement in fluorescence intensity ⁇ % MFI level in cells having reacted with anti - human CAPRIN - 1 antibody - MFI level of control / MFI level of control x 100.
  • the cytotoxic activity was determined as a cytotoxic activity against a cancer cell line. Specifically, as described above, the result was obtained by mixing an anti-CAPRIN-1 antibody to be used in the present invention, a rabbit lymphocyte, and 10 3 cells of each cancer cell line that had incorporated chromium-51, culturing cells for 4 hours, measuring the amount of chromium-51 released in medium after culture, and then calculating the cytotoxic activity against the cancer cell line by the following formula *.
  • cytotoxic activity % (the amount of chromium - 51 released from cancer cells upon addition of anti - CAPRIN - 1 antibody and rabbit lymphocyte ) / the amount of chromium - 51 released from target cells to which 1 ⁇ N hydrochloric acid had been added x 100.
  • the anti-CAPRIN-1 mouse monoclonal antibodies #1, #2, and #3 were evaluated for their cytotoxic activity against cancer cells.
  • Each cell culture supernatant producing #1, #2, or #3 was purified using Hitrap ProteinA Sepharose FF (GE HealthCare), subjected to buffer replacement with PBS(-), and then filtered with a 0.22 ⁇ m filter (Millipore). The resultants were used as antibodies for activity measurement.
  • 10 6 cells of the MDA-MB-157 human breast cancer cell line were collected in a 50-ml centrifuge tube, 100 ⁇ Ci chromium-51 was added, and then incubation was performed at 37°C for 2 hours.
  • the resultant was washed three times with RPMI1640 medium containing 10% FBS.
  • Cells were added to a 96-well V-bottom plate at 10 3 cells per well for use as target cells.
  • the above purified antibodies (1 ⁇ g each) were added to the cells.
  • 5x10 4 cells of mouse splenocytes isolated from the spleen of a 6-week-old BALB/C mouse (Japan SLC Inc.) according to a conventional method were further added and then cultured for 4 hours at 37°C under conditions of 5% CO 2 . After culture, the amount of chromium-51 released from damaged tumor cells in a culture supernatant was measured, and the cytotoxic activity of each anti-CAPRIN-1 antibody against cancer cells was calculated.
  • the antibodies #1, #2, and #3 were examined for their cytotoxic activity against other cancer cells including glioma cell lines T98G and U373, lung cancer cell lines A549 and QG56, renal cancer cell lines Caki-1 and ACHN, a uterine cervix cancer cell line SW756, a bladder cancer cell line T24, an esophageal cancer cell line KYSE180, gastric cancer cell lines MKN28 and MKN45, a colorectal cancer cell line SW480, a leukemia cell line AML5, and a lymphoma cell line Ramos.
  • glioma cell lines T98G and U373, lung cancer cell lines A549 and QG56 renal cancer cell lines Caki-1 and ACHN
  • a uterine cervix cancer cell line SW756, a bladder cancer cell line T24 an esophageal cancer cell line KYSE180
  • gastric cancer cell lines MKN28 and MKN45 gastric cancer cell lines
  • SW480 colorectal cancer cell
  • the antibody #1 exhibited 11.2% activity against T98G (2.5% in the case of isotype control), 13.3% activity against U373 (4.3% in the case of isotype control), 20.8% activity against A549 (4.5% in the case of isotype control), 21.3% activity against QG56 (5.3% in the case of isotype control), 15.9% activity against Caki-1 (4.5% in the case of isotype control), 14.7% activity against ACHN (3.8% in the case of isotype control), 13.5% activity against SW756 (5.1% in the case of isotype control), 11.6% activity against T24 (3.8% in the case of isotype control), 16.2% activity against KYSE180 (3.7% in the case of isotype control), 12.8% activity against MKN28 (4.2% in the case of isotype control), 13.4% activity against MKN45 (4.6% in the case of isotype control), 12.4% activity against SW480 (6.4% in the case of isotype control), 10.3% activity against AML5 (4.7% in the case of isotype control),
  • the cytotoxic activity was determined as a cytotoxic activity against a cancer cell line. Specifically, as described above, the result was obtained by mixing an anti-CAPRIN-1 antibody to be used in the present invention, a mouse splenocyte, and 10 3 cells of each cancer cell line that had incorporated chromium-51, culturing cells for 4 hours, measuring the amount of chromium-51 released in medium after culture, and then calculating the cytotoxic activity against the cancer cell line by the following formula*.
  • cytotoxic activity % the amount of chromium - 51 released from cancer cells upon addition of anti - CAPRIN - 1 antibody and a mouse splenocyte / the amount of chromium - 51 released from target cells to which 1 ⁇ N hydrochloric acid had been added x 100.
  • the obtained anti-CAPRIN-1 mouse monoclonal antibodies #1 and #2 were evaluated for their cytotoxic activity (CDC activity) against cancer cells.
  • Blood collected from a rabbit was added to an Eppendorf tube, left to stand at room temperature for 60 minutes, and then subjected to 5 minutes of centrifugation at 3000 rpm.
  • serum for measurement of CDC activity was prepared.
  • 10 5 cells of the MDA-MB-231 V human breast cancer cell line were collected in a 50-ml centrifuge tube, 100 ⁇ Ci chromium-51 was added, and then incubation was performed at 37°C for 2 hours. The resultant was washed three times with RPMI medium containing 10% FBS.
  • the cells were suspended in RPMI medium containing the above-prepared rabbit serum (50%), and then added to a 96-well V-bottom plate at 10 3 cells per well. 1 ⁇ g each of the mouse monoclonal antibodies #1 and #2 was added to the cells and then cells were cultured for 4 hours at 37°C under conditions of 5% CO 2 . After culture, the amount of chromium-51 released from damaged tumor cells in a culture supernatant was measured, and then the CDC activity of each antibody against MDA-MB-231V was calculated. As a result, the antibodies #1 and #2 both exhibited more than 21 % CDC activity. Also, no cytotoxic activity was observed in a negative control group to which no antibody had been added. Therefore, it was revealed that the antibodies #1 and #2 can damage tumor cells expressing CAPRIN-1 also by CDC activity.
  • the cytotoxic activity was determined as a cytotoxic activity against a cancer cell line. Specifically, as described above, the result was obtained by mixing an anti-CAPRIN-1 antibody to be used in the present invention, serum, and 10 3 cells of each cancer cell line that had incorporated chromium-51, culturing cells for 4 hours, measuring the amount of chromium-51 released in medium after culture, and then calculating the cytotoxic activity against the cancer cell line by the following formula*.
  • cytotoxic activity % the amount of chromium - 51 released from cancer cells upon addition of an anti - CAPRIN - 1 antibody and serum / ( the amount of chromium - 51 released from target cells to which 1 ⁇ N hydrochloric acid had been added ) x 100.
  • anti-CAPRIN-1 mouse monoclonal antibodies #1 and #2 were evaluated for their in vivo anti-tumor effects in tumor-bearing mice.
  • Antibodies used herein were prepared by column purification of the culture supernatant of each cell producing #1 or #2 in the same manner as described above.
  • the anti-tumor effects of the antibodies #1 and #2 were examined using tumor-bearing mice into which a mouse-derived cancer cell line expressing CAPRIN-1 had been transplanted.
  • 4T1 cells purchased from ATCC
  • 4T1 cells were transplanted subcutaneously to the dorsal region of 30 Balb/c mice (Japan SLC Inc.) at 5 ⁇ 10 5 cells/mouse. Tumors were allowed to grow to reach a size of about 5 mm in diameter.
  • the antibodies #1 and #2 were administered intraperitoneally to 20 tumor-bearing mice from among the 30 tumor-bearing mice in an amount of 200 ⁇ g (200 ⁇ l)/mouse (each antibody was administered to 10 mice).
  • the same amount of the antibody was administered intraperitoneally to each tumor-bearing mouse 3 times in total within 2 days. Tumor sizes were measured every day and anti-tumor effects were examined by observation. Meanwhile, as a control group, PBS (-) was administered instead of the antibodies to the remaining 10 tumor-bearing mice.
  • the tumor size was calculated as a volume using the formula: length of major axis x length of minor axis x length of minor axis x 0.5.
  • the antibodies of the present invention are useful for treating and/or preventing a cancer.
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US20130045210A1 (en) 2013-02-21
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PL2532366T3 (pl) 2017-02-28
EP2532366B1 (fr) 2016-09-07
CA2788716C (fr) 2019-06-18
ES2606173T3 (es) 2017-03-23
KR101801667B1 (ko) 2017-11-27
BR112012018951A2 (pt) 2017-06-27
MX342291B (es) 2016-09-23
BR112012018951B1 (pt) 2020-05-12
BR112012018951B8 (pt) 2020-06-09
KR20130033347A (ko) 2013-04-03
WO2011096533A1 (fr) 2011-08-11
BR112012018951A8 (pt) 2020-03-31
AU2011211698B2 (en) 2015-07-16
AU2011211698A1 (en) 2012-08-23
CN102821789B (zh) 2016-03-02
HUE030742T2 (en) 2017-06-28
CN102821789A (zh) 2012-12-12
PT2532366T (pt) 2016-12-20
EP2532366A4 (fr) 2013-12-04
DK2532366T3 (da) 2017-01-02

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