EP2373332A1 - Process for production of vaccines - Google Patents

Process for production of vaccines

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Publication number
EP2373332A1
EP2373332A1 EP09775142A EP09775142A EP2373332A1 EP 2373332 A1 EP2373332 A1 EP 2373332A1 EP 09775142 A EP09775142 A EP 09775142A EP 09775142 A EP09775142 A EP 09775142A EP 2373332 A1 EP2373332 A1 EP 2373332A1
Authority
EP
European Patent Office
Prior art keywords
toxin
vaccine
toxoid
toxins
genus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09775142A
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German (de)
English (en)
French (fr)
Inventor
Jessica Reineke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Vetmedica GmbH
Boehringer Ingelheim Animal Health USA Inc
Original Assignee
Boehringer Ingelheim Vetmedica GmbH
Boehringer Ingelheim Vetmedica Inc
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Application filed by Boehringer Ingelheim Vetmedica GmbH, Boehringer Ingelheim Vetmedica Inc filed Critical Boehringer Ingelheim Vetmedica GmbH
Priority to EP09775142A priority Critical patent/EP2373332A1/en
Publication of EP2373332A1 publication Critical patent/EP2373332A1/en
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/08—Clostridium, e.g. Clostridium tetani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/12—Antidiarrhoeals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Definitions

  • the invention relates to a process of production of vaccines, and vaccines produced accordingly.
  • Clostridium difficile a spore-forming, gram-negative bacterium, is responsible for 60% of the cases of antibiotics-associated diarrhoea and for almost 100% of the patients affected by pseudomembranous colitis. The mechanism responsible for the outbreak of the disease is not yet fully understood. It might be related to both host and strain factors, since not all patients infected with C. difficile develop the disease. Clinical symptoms of infected patients can range from being asymptomatic to life-threatening toxic megacolon.
  • C. difficile like many other pathogens causing disease in animals, including humans, produces toxins.
  • a toxin is a poisonous substance produced by living cells or organisms that is active at very low concentrations. Toxins can be small molecules, peptides, or proteins and are capable of causing disease on contact or absorption with body tissues by interacting with biological macromolecules such as enzymes or cellular receptors.
  • C. difficile produces two toxins, Toxin A (TcdA) and Toxin B (TcdB) which are causative for antibiotic-associated diarrhoea or pseudomembranous colitis.
  • TcdA and TcdB are single-chained proteins characterized by a tripartite functional organization.
  • the hydrophobic middle part is a putative translocation domain and the N-terminal catalytic domain of the proteins carries the glycosyltransferase site.
  • the uptake process into the cytosol of the target cell has not yet been fully characterized. However, it is generally accepted that the toxins are endocytosed after binding to cell surface receptors. After acidification of the endosomes, only the N-terminal domain of the toxin translocates into the cytosol. This translocation process is supposedly mediated by pore formation, since TcdA forms pores in artificial membranes at low pH.
  • Activation of the toxin requires proteolytic cleavage between the amino acids Leu543 and Gly544, which liberates a small fragment of 63 kDa that harbours the N-terminal catalytic domain into the cytosol.
  • the N-terminal 63 kDa fragment displays full cytotoxic activity.
  • GTPases of the Rho/Rac family These proteins are involved in many cellular functions, e.g. organization of the actin cytoskeleton, control of transcription, cell polarity and proliferation. Since Rho GTPases play an important role in many functions of the immune system, including pathogen defense responses, cytokine expression and signalling of immune cells, they constitute optimal targets for bacterial toxins.
  • Vaccines comprising recombinantly expressed polypeptides representing the C-terminal ligand domain of TcdA or TcdB are disclosed in WO9859053, WO0061761 , WO0061762, WO9702836, Pavliakova et al, Infect Immun (2000), 68(4), 2161-2166; Ward et al, Infect Immun (1999), 67(10), 5124- 5132; and Lyerly et al., Current Microbiol 21 : 29-32).
  • WO2007146139 discloses a codon- optimised DNA-molecule encoding the receptor binding domain of TcdA and TcdB and is use as a DNA vaccine.
  • WO2004041857 discloses non-toxic mutants of TcdB and its use for vaccination.
  • Genth, H. et al., Infect. Immun. (2000), 68: 1094-1 101 discloses a method to generate enzymatically deficient Clostridium difficile toxin B as an antigen for immunization.
  • Vaccines are typically manufactured by making a preparation comprising antigenic components of such pathogens and admixing them with a pharmaceutically acceptable carrier. To achieve an effective immune response and for economic reasons it is desirable to use preparations obtained from bacterial cultures with only few processing or fractionation steps. In the case of toxin producing organisms, the problem is that the toxins included in such preparations would prevent administration thereof unless they are inactivated. Therefore, the prior art approaches have suggested to make vaccines against bacterial pathogens producing AB toxins like C. difficile by chemical inactivation of the toxins, recombinant expression of the non-toxic domain of the toxins (the C-terminal receptor binding or "B" domain), or producing non-toxic mutants of the toxins. However, all these measures lead to a loss of antigenic epitopes of the pathogenic organism which may affect the effectivity of the vaccine, and/or are costly.
  • step (c) Combining the composition of step (b) with a pharmaceutically acceptable carrier.
  • the enzymatic cleavage is autocatalytic.
  • inositol phosphate preferably inositol hexaphosphate is used as a co-factor of the enzymatic cleavage.
  • the invention relates to a process as described, wherein the cells are separated from the culture medium after the harvest, and the AB toxin in the culture medium is cleaved.
  • the process of the invention can be used for the production of vaccines against pathogens of the genus Clostridium, preferably C. difficile, C. sordellii, C. botulinum, C. perfringens, C. tetani, or C. novyi, or of the genus Vibrio, preferably V. cholerae, V. parahaemolyticus, V. vulnificus, or V. spectacularus, or V. anguillarum or of the genus Xenorhabdus, preferably X. nematophila, X. bovienii, or of the genus Yersinia, preferably Y. pseudotuberculosis, Y. pestis, Y.
  • enterocolitica or Y. mollaretti, or of the genus Bordetella, preferably B. pertussis, B. parapertussis, or B. bronchiseptica, or of the genus Actinobacillus, preferably A. pleuropneumoniae, or A. suis and E. coli.
  • the present invention relates to a vaccine produced with the process as described.
  • Another aspect of the invention is related to the use of a vaccine produced according to the process as disclosed, for the vaccination of animals, including humans, against infection of bacterial pathogens producing AB toxins.
  • Another aspect of the invention relates to a method of vaccination of animals, including humans, against infection of bacterial pathogens producing AB toxins, comprising administering an effective amount of a vaccine produced according to the process of the invention to an animal, including a human.
  • the toxic A domain is separated from the transporter domain B and looses its ability to enter the cytosol of cells where it needs to be to exert its toxic effects.
  • the resulting composition is not toxic any more, or much less toxic than the single chain AB toxin, when applied to living organisms.
  • this kind of inactivation preserves the natural conformation and the antigenic epitopes of the proteins which is important for the effectivity of the vaccine.
  • AB toxin is used for a single-chain bacterial toxin which, like the LCT's, comprises a catalytic domain (the A domain) and a receptor binding/translocation domain (the B domain or transporter domain), and wherein the activation of the catalytic domain in vivo occurs by autocatalytic cleavage releasing the catalytic domain into the cytosol.
  • AB toxins are for example the LCT's including toxin A (TcdA) und B (TcdB) of Clostridium difficile, the lethal (TcsL) und the hemorrhagic toxin (TcsH) of Clostridium sordellii, and the ⁇ -Toxin (Tcn ⁇ ) of Clostridium novyi.
  • AB toxins include the RTX toxins of Vibrio cholerae (VcRTX), V. vulnificus (VvRtx), V. spectacularus (VsRtx), Xenorhabdus nematophila (XnRtx), X.
  • XbRtx Yersinia pseudotuberculosis
  • YpRtx Y. mollaretti
  • YmMfp2 / Y. enterocolitica
  • YST Listonella anguillarum
  • VaRtx Bordetella pertussis
  • a vaccine is a pharmaceutical preparation which is used to improve immunity to a particular disease in animal, including humans.
  • Vaccines can be prophylactic (e.g. to prevent or ameliorate the effects of a future infection by any natural or "wild" pathogen), or therapeutic, i.e. applied in a situation where the host is already infected by the pathogen, with or without clinical symptoms of disease.
  • Vaccines may contain killed micro organisms, modified live (attenuated) micro organisms, antigenic subunit preparations of micro organisms (e.g.
  • the vaccine may contain an adjuvant, an agent that can stimulate the immune system and increase the response to a vaccine, without having any specific antigenic effect in itself.
  • adjuvants are alum (hydrated aluminium potassium sulfate), aluminium phosphate, aluminium hydroxide, squalene, or oil-based adjuvants.
  • a preferred adjuvant is the commercially available Carbopol ® 934P (Carbomer 934P; Noveon, Inc. , Pedricktown, NJ, USA) which may be present in the amount of about 2 ml/I.
  • Carbopol is an acrylic acid polymer which is cross-linked with polyallylsucrose.
  • the first step of the process is culturing the pathogen under conditions where the AB toxin is produced.
  • Bacterial cell culture is well-established in the art. Standard methods for the different species are known and suitable samples of the microorganisms are available from public collections. Listed microorganisms are cultivated according to their special requirements. Clostridia are cultivated under anaerobic atmosphere whereas Yersinia, Xenorhabdus, Bordetella and Vibrio can be cultivated aerobically. Yersinia are psychrotolerant, these organisms are usually cultivated at 28°C. Each organism needs its special medium composition for growth which is readily known in the art.
  • the AB toxin is normally released into the culture medium in the late stationary phase of the culture. After the harvest, it is often advantageous to separate the cells from the culture medium, as the AB toxins are present in the medium in sufficient concentration. This can be done by centrifugation. When the cells are separated by centrifugation, they are discarded and the supernatant is further processed. The toxins in the medium are then deactivated by enzymatic cleavage taking advantage of their autocatalytic properties. To achieve cleavage, the conditions have to be adjusted properly to allow for the enzymatic activity. Most importantly, a co-factor has to be added which promotes the enzymatic activity.
  • Inositol phosophate in particular inositol hexaphosphate, may be used as a co-factor at a concentration range of 1 ⁇ mol/l to 10 mmol/l, more preferably 10-100 ⁇ mol/l, but other analogues or derivates such as 1 ,3,4- or 3,4,6-triphopsphate, 1 ,2,3,4-, 1 ,3,4,5-, 3,4,5,6, or 1 ,4,5,6-tetraphosphate, or 1 ,2,3,4,5-, 1 ,2,3,5,6-, 1 ,3,4,5,6-, 2,3,4,5,6- pentaphosphate work as well, but may require higher concentrations.
  • the pH should be in the range of 6,5-8,5, and the pH of the culture medium is normally already within that range. Otherwise, a buffer may be added or exchanged e.g. by dialysis or ultrafiltration, e.g. Tris HCI at pH 8.5. A suitable temperature range is 20 - 40 0 C. The cleavage will normally be completed within 1 to 24 hours and should be tested with an assay like those disclosed in the examples, to avoid residual toxicity.
  • the toxin species may be purified from the harvest and/or culture medium prior to inactivation. If the pathogen produces more than one toxin species, these toxin species may be isolated from each other before inactivation.
  • C. difficile produces the two toxins Toxin A (TcdA) and Toxin B (TcdB), as outlined above. These two proteins may be separated as exemplified in Example 4 herein before inactivation, and then used in vaccine preparation either individually, or in combination.
  • Toxoid A and/or Toxoid B of C. difficile i.e. Toxin A and/or Toxin B inactivated according to the present invention, are preferred antigens for vaccinations.
  • the toxoids may be further purified after autocatalytic cleavage, with enrichment of the larger, C-terminal cleavage fragments (e.g. consisting of amino acids 543-2710 of the holotoxin A, or amino acids 544-2666 or 545-2666 of the holotoxin B).
  • the purified toxoids of the invention may further be combined with inactivated AB toxins of other pathogens in vaccine preparations.
  • the resulting preparation is then brought into its final formulation for use as a vaccine as deemed appropriate.
  • it may be used as is, with the aqueous environment as is being the pharmaceutically acceptable carrier.
  • the environment may also be changed, for example by dilution, dialysis, ultrafiltration or further purification steps like affinity chromatography.
  • the antigen preparation may be freeze-dried for storage, for reconstitution with water before use. If appropriate, adjuvants may be added.
  • Adjuvants that can be considered are for example water in oil-, oil in water-, multiphasic- or non- mineral oil- emulsions, aluminium based adjuvants, polymeric adjuvants like Carbopol®, squalene, liposome, microparticles, immunostimulatory complexes and Toll-like receptor cascade activating adjuvants.
  • the vaccine will be administered by subcutan, interdermal, intramuscular, intravenous or intraperitoneal injection. The frequency of injection and dosage depends on the target species. Susceptible species are humans, dogs, cats, rabbits, pigs, cattle, fish, rodents and horses.
  • the vaccination is a prophylactic treatment and protection of progeny can be achieved by vaccination of the mother. The time of vaccination starts after disappearance of maternal antibodies and may require booster vaccinations after 4 weeks and at later time points.
  • the present invention relates to a vaccine against Clostridium- induced diarrhoea, comprising toxoid A and/or toxoid B of Clostridium difficile, wherein said toxoid A and/or toxoid B has been generated from Toxin A and/or Toxin B by autocatalytic cleavage, optionally together with a pharmaceutically acceptable carrier.
  • Toxoid A consists of amino acids 543-2710 of the sequences as deposited in public databases (EMBL, NCBI) under the accession Nos. YP_001087137, ZP_05349827, or YP_003213641.
  • Example 1 Manufacture of a Clostridium difficile vaccine
  • the culture is harvested and the bacteria are sedimented by centrifugation at 8000 x g for 10 minutes
  • the supernatant is used as is, or toxins may be enriched by gel permeation chromatography (e.g. on S300 Sephacryl), affinity chromatography, anion exchange chromatography and/or ultrafiltration.
  • the reducing agent dithiotreitol is then added at a final concentration of 1-50 mmol/l to the supernatant or toxin-enriched preparation, followed by addition of the chelate forming agent ethylene diamine tetraacetate at a final concentration of 10-100 mmol/l.
  • the resulting preparation is then brought into its final formulation for use as a vaccine as deemed appropriate.
  • a vaccine for example, it may be used as is, with the aqueous environment as is being the pharmaceutically acceptable carrier.
  • the environment may also be changed,for example by dilution, dialysis ultrafiltration or further purification steps like affinity chromatography.
  • adjuvants may be added.
  • Adjuvants that can be considered are for example water in oil-, oil in water-, multiphasic- or non-mineral oil- emulsions, aluminium based adjuvants, polymeric adjuvants like Carbopol, squalene, liposome, microparticles, immunostimulatory complexes and Toll-like receptor cascade activating adjuvants.
  • the vaccine will be administered by subcutan, interdermal, intramuscular, intravenous or intraperitoneal injection.
  • the frequency of injection and dosage depends on the target species. Susceptible species are humans, dogs, cats, rabbits, pigs, cattle and horses.
  • the vaccination is a prophylactic treatment and protection of progeny can also be achieved by vaccination of the mother. The time of vaccination starts after disappearance of maternal antibodies and may require booster vaccinations after 4 weeks and at later time points.
  • CHO cells Choinese hamster ovary, e.g. DSM ACC1 10, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany
  • 96 or 24 well microtiter plates 100 ⁇ l per well
  • FCS fetal calf serum
  • Cells treated with the vaccine preparation should show no morphological changes, as those of the negative control.
  • Cells treated with untreated culture supernatant will show a cytopathic effect which is primarily characterized by getting a round shape and developing an "astrocyte-like" morphology. If the vaccine-treated cells show a cytopathic effect similar to the positive control, the enzymatic cleavage was not complete and has to be repeated.
  • AB-toxins of Clostridium difficile were inactivated by use of their intrinsic autocatalytic cleavage function and used as vaccines in animals.
  • 1 ml of C. difficile (reference strain VP110463, ATCC 43255) working culture was transferred into a pretreated and sterile dialysis bag containing 200 ml 0.9 % NaCI.
  • the dialysis bag was placed into 1.3 I BHI medium and was incubated for 5 d at 37 0 C in an anaerobic chamber. After 5 d the content of the dialysis bag was centrifuged (5000 rpm, 4 0 C, 15 min) and fractionated ammonium persulfate precipitation of the supernatant was performed.
  • the first precipitation step (toxin A) was performed by addition of 45 % (NH) 4 SO 4 and stirring for 3 h at 4 0 C. After this time the solution was centrifuged (5000 rpm, 4 0 C and 30 min) and a second precipitation step (toxin B) was conducted by addition of (NH) 4 SO 4 to a final content of 70 %. The second fraction was stirred for 3 h at 4 0 C and after that again centrifuged (5000 rpm, 4 0 C and 30 min). The resulting pellets of the precipitation steps were suspended in 5 ml 50 mM Tris/HCI pH 7.5.
  • sucrose density gradient centrifugation The two fractions (toxin A and B) received from (NH) 4 SO 4 precipitation were further purified by sucrose density gradient centrifugation. Therefore a sucrose density gradient was prepared by underlying 4.5 ml of the following sucrose solutions in increasing order in an ultracentrifuge tube: 10 %, 18.75 %, 27.50 %, 36.25 % and 45 % sucrose in 50 mM Tris/HCI pH 7.5.
  • the toxin fractions were added on the top and the tube was centrifuged for 3.5 h at 4 0 C and 100.000 g. The resulting gradient was collected in 2 ml aliquots. The toxin content of the samples was measured by cytotoxicity assays with CHO-K1 cells.
  • CHO-K1 cells ATCC CCL-61 ) were grown in DMEM/FIO-Medium containing 10 % FCS, 0.5 % L-glutamine and 0.5 % penicillin/streptomycin. Monolayers of cells (about 4000 per well) were prepared in 96-well microtiter plates and incubated at 37 0 C and 5 % CO 2 for 24 h. After incubation time 10- fold dilutions of toxin containing samples were prepared. After removal of medium from cells, toxin dilutions were added. Cytotoxic effects were examined microscopically after 24 h by an inverted microscope.
  • the toxin containing fractions of the sucrose density gradient centrifugation were pooled (each for Toxin A and Toxin B) and 1 :2 diluted with 50 mM Tris/HCI pH 7.5.
  • the samples (either Toxin A or Toxin B) were loaded on an ion exchange column and a NaCI gradient ranging from 50 mM NaCI in 50 mM Tris/HCI pH 7.5 to 700 mM NaCI in 50 mM Tris/HCI pH 7.5 was conducted ( ⁇ NaCI 5 mM/ml) to elute the toxin. Fractions of 2 ml were collected. Toxin content of the samples was measured by cytotoxicity assays with CHO- K1 cells.
  • the toxin containing fractions were pooled and concentrated by an ultra centrifugation step for 15 min at 4 0 C and 5000 rpm. To the obtained toxin solutions 20 % glycerin was added and samples were stored at -20 0 C.
  • Purification steps were also monitored by SDS-PAGE. Proteins were visualized by Coomassie staining. Concentration of the final toxin samples were determined by comparison of the toxin amount in SDS gels with BSA standards. The comparison was conducted by optical adjustment and by computer analysis.
  • Vaccine preparations were prepared by induction of autocatalytic cleavage of toxins by adding DTT and IP 6 - Toxin A cleavage was performed in H 2 O at a final volume of 50 ⁇ l by addition of 3 mM IP 6 and 50 mM DTT. Toxin B cleavage was performed in H 2 O at a final volume of 100 ⁇ l by addition of 1 mM IP 6 and 150 mM DTT. Autocleavage was performed over night at 37 0 C on a rotator.
  • the resulting cleavage products were analyzed by cytotoxicity assay and SDS PAGE analysis. Cytotoxicity assays were performed with CHO-K1 cells and Caco cells as Caco cells showed higher sensitivity against Toxin A. Caco cells were grown in MEM medium containing 10 % FCS and 0.5 % penicillin/streptomycin on 96 microtiter wells incubated at 37 0 C and 5 % CO 2 for 48 h. The cytotoxicity assay showed a reduction of cytotoxicity of at least 10 3 fold for toxin A and 10 4 fold for toxin B after 24 h. Cleavage efficiency was visualized with Coomassie stained SDS PAGE analysis.
  • Vaccine doses with respective inactivated toxins were prepared with the Sigma Adjuvant System (oil-in-water emulsion with Monophosphoryl Lipid A and synthetic trehalose dicorynomycolate). Toxoid concentrations were determined by comparison of the toxin amount in SDS gels with BSA standards. The comparison was conducted by optical adjustment and by computer analysis. The adjuvant was reconstituted as described by the manufacturer with PBS and mixed 1 :1 with the respective toxoid sample.
  • the classical model organism for Clostridium difficile infection is the Syrian hamster.
  • Syrian hamsters react very sensitive to the infection and develop clinical signs and pathological alterations similar to those in humans.
  • the hamster infection model is a very strict model ending in a 100 % mortality of infected animals.
  • CHO-K1 cells were seeded at 5000 cells/well in 96 well plates in DMEM/FIO-mdeium containing 10 % FCS, 0,5 % L-glutamine and 0,5 % penicillin/streptomycin and were incubated at 37°C and 5 % CO 2 overnight. Dilutions of hamster sera were prepared in medium and incubated for 1 h at 37 0 C with toxin A and B dilutions.
  • the toxins had been diluted to concentrations that cause > 90 % rounding of cells after 3 h and 24 h.
  • Cell rounding was determined microscopically after 3 h and 24 h as described before.
  • Neutralization titer was defined as the reciprocal value of the greatest sera dilution which completely inhibited rounding of cells after 24 h.
  • the objective of the study was to determine if the repeated subcutaneous immunizations with different doses of a combination of inactivated toxin A and B is biocompatible and if protection against a C. difficile infection can be induced. Therefore 12 male Syrian hamsters were used that were randomized to 4 respective groups at the arrival at the test facility. Each group consisted of 3 animals. The groups were vaccinated on different days and increasing doses in order to be able to react to potential toxic effects after vaccination. study outline: All animals were without clinical signs after subcutaneous vaccination thus the toxin dose could be increased to up to 4 ⁇ g each for toxoid A and B.

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EP09775142A 2008-12-03 2009-12-01 Process for production of vaccines Withdrawn EP2373332A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09775142A EP2373332A1 (en) 2008-12-03 2009-12-01 Process for production of vaccines

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08170591 2008-12-03
PCT/EP2009/066109 WO2010063693A1 (en) 2008-12-03 2009-12-01 Process for production of vaccines
EP09775142A EP2373332A1 (en) 2008-12-03 2009-12-01 Process for production of vaccines

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EP2373332A1 true EP2373332A1 (en) 2011-10-12

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US (1) US20110243991A1 (ru)
EP (1) EP2373332A1 (ru)
JP (1) JP2012510497A (ru)
KR (1) KR20110100189A (ru)
CN (1) CN102238960B (ru)
AR (1) AR074455A1 (ru)
AU (1) AU2009324180A1 (ru)
BR (1) BRPI0922219A2 (ru)
CA (1) CA2737403A1 (ru)
CL (1) CL2011001284A1 (ru)
CO (1) CO6390040A2 (ru)
MX (1) MX2011005758A (ru)
RU (1) RU2011126602A (ru)
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UA (1) UA105508C2 (ru)
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ZA (1) ZA201101645B (ru)

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CA2659684A1 (en) * 2006-08-02 2008-02-07 Johannes Gutenberg-Universitaet Mainz Medicament for lct poisoning
EP2198007B1 (en) 2007-09-14 2017-10-25 Sanofi Pasteur Biologics, LLC Pharmaceutical compositions containing clostridium difficile toxoids a and b
EP4365196A3 (en) * 2011-04-22 2024-08-07 Wyeth LLC Compositions relating to a mutant clostridium difficile toxin and methods thereof
BR122016023101B1 (pt) 2012-10-21 2022-03-22 Pfizer Inc Polipeptídeo, composição imunogênica que o compreende, bem como célula recombinante derivada de clostridium difficile
US8909605B1 (en) 2013-02-28 2014-12-09 Emc Corporation Method and system for accelerating data movement using change information concerning difference between current and previous data movements
EP2988778A4 (en) * 2013-04-22 2016-12-14 Board Of Regents Of The Univ Of Oklahoma CLOSTRIDIUM DIFFICILE IMPREGENT AND METHOD OF USE
CN109865135A (zh) * 2019-03-18 2019-06-11 宁波大学 一种银鲳美人鱼发光杆菌和灿烂弧菌联合灭活疫苗
KR102376876B1 (ko) * 2020-04-09 2022-03-21 대진대학교 산학협력단 무독성의 클로스트리디움 디피실 독소단백질-단편 및 이의 용도

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6967088B1 (en) * 1995-03-16 2005-11-22 Allergan, Inc. Soluble recombinant botulinum toxin proteins
EP0877623A1 (en) * 1995-07-07 1998-11-18 Oravax, Inc. Intranasal vaccination against gastrointestinal disease
EP2502998A3 (en) * 1999-04-09 2013-02-27 Intercell USA, Inc. Recombinant toxin A/ToxinB vaccine against clostridium difficile
AU2003299527A1 (en) * 2002-06-17 2004-06-07 Jimmy D. Ballard Mutant of clostridium difficile toxin b and methods of use
US8852600B2 (en) * 2006-06-08 2014-10-07 The Rockefeller University Codon-optimized DNA molecules encoding the receptor binding domains of Clostridium difficile toxins A and B, and methods of use thereof
CA2659684A1 (en) * 2006-08-02 2008-02-07 Johannes Gutenberg-Universitaet Mainz Medicament for lct poisoning

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIBBY J M ET AL: "EFFECTS OF THE TWO TOXINS OF CLOSTRIDIUM DIFFICILE IN ANTIBIOTIC- ASSOCIATED CECITIS IN HAMSTERS", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 36, no. 2, 1 May 1982 (1982-05-01), pages 822 - 829, XP002916723, ISSN: 0019-9567 *

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MX2011005758A (es) 2011-06-28
RU2011126602A (ru) 2013-01-10
TW201026852A (en) 2010-07-16
ZA201101645B (en) 2011-11-30
KR20110100189A (ko) 2011-09-09
US20110243991A1 (en) 2011-10-06
AR074455A1 (es) 2011-01-19
UA105508C2 (ru) 2014-05-26
SG171934A1 (en) 2011-07-28
AU2009324180A1 (en) 2010-06-10
WO2010063693A1 (en) 2010-06-10
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CA2737403A1 (en) 2010-06-10
BRPI0922219A2 (pt) 2018-10-23

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