EP2286227A1 - Procédé de détection d'espèces chimiques ou biologiques, et arrangement d'électrodes destiné à ce procédé - Google Patents

Procédé de détection d'espèces chimiques ou biologiques, et arrangement d'électrodes destiné à ce procédé

Info

Publication number
EP2286227A1
EP2286227A1 EP09757475A EP09757475A EP2286227A1 EP 2286227 A1 EP2286227 A1 EP 2286227A1 EP 09757475 A EP09757475 A EP 09757475A EP 09757475 A EP09757475 A EP 09757475A EP 2286227 A1 EP2286227 A1 EP 2286227A1
Authority
EP
European Patent Office
Prior art keywords
electrode
species
molecule
electrodes
redox reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP09757475A
Other languages
German (de)
English (en)
Other versions
EP2286227B1 (fr
Inventor
Eric Nebling
Joerg Albers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Original Assignee
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV filed Critical Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Publication of EP2286227A1 publication Critical patent/EP2286227A1/fr
Application granted granted Critical
Publication of EP2286227B1 publication Critical patent/EP2286227B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/404—Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid, e.g. Clark-type oxygen sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/30—Electrochemically active labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention porte sur un procédé de détection d'une ou plusieurs espèces chimiques ou biologiques, apte(s) à subir une réaction redox ou bien pouvant générer directement ou indirectement une molécule apte à subir une réaction redox, le courant généré par ladite réaction redox étant détecté par au moins une électrode. Le procédé comprenant les étapes suivantes : 1. positionnement d'une certaine quantité, variant dans le temps, de l'espèce ou de la molécule, contre ou au voisinage de ladite au moins une électrode, pendant un temps t1-t2, 2. commutation répétée de ladite au moins une électrode pendant le temps t1-t2 entre deux potentiels différents, de façon que l'électrode prenne par rapport à une électrode de référence des potentiels se trouvant dans la zone du potentiel d'oxydation des espèces mentionnées ou de la molécule mentionnée, ou au-delà, ou dans la zone du potentiel de réduction des espèces mentionnées ou de la molécule mentionnée, ou en-deçà, ce qui va provoquer en alternance une réduction et une oxydation des espèces mentionnées de la molécule mentionnée, et 3. détection de l'oxydation et de la réduction, répétées pendant le temps t1-t2, des espèces/de la molécule, dans le courant formé par l'au moins une électrode. Par ailleurs, l'invention fournit un réseau d'électrodes pour la mise en oevre de ce procédé, comprenant au moins une position de mesure et au moins une électrode de mesure par position de mesure ainsi qu'une électrode de référence, le réseau d'électrodes étant configuré de telle sorte que l'électrode de mesure puisse être, par rapport à l'électrode de référence, montée en alternance en tant que cathode et en tant qu'anode.
EP09757475.0A 2008-06-06 2009-05-29 Procédé de détection d'espèces chimiques ou biologiques, et dispositif destiné à ce procédé Active EP2286227B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008027038A DE102008027038A1 (de) 2008-06-06 2008-06-06 Verfahren zum Detektieren von chemischen oder biologischen Species sowie Elektrodenanordnung hierfür
PCT/EP2009/056616 WO2009147093A1 (fr) 2008-06-06 2009-05-29 Procédé de détection d'espèces chimiques ou biologiques, et arrangement d'électrodes destiné à ce procédé

Publications (2)

Publication Number Publication Date
EP2286227A1 true EP2286227A1 (fr) 2011-02-23
EP2286227B1 EP2286227B1 (fr) 2017-12-13

Family

ID=41023150

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09757475.0A Active EP2286227B1 (fr) 2008-06-06 2009-05-29 Procédé de détection d'espèces chimiques ou biologiques, et dispositif destiné à ce procédé

Country Status (6)

Country Link
US (1) US8900440B2 (fr)
EP (1) EP2286227B1 (fr)
JP (1) JP5675594B2 (fr)
CN (1) CN102057273B (fr)
DE (1) DE102008027038A1 (fr)
WO (1) WO2009147093A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011179893A (ja) * 2010-02-26 2011-09-15 Jfe Steel Corp 金属内部への侵入水素量の測定方法および移動体の金属部位内部へ侵入する水素量のモニタリング方法
EP2390664B1 (fr) * 2010-05-25 2013-04-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Procédé de détection électrochimique de réactions de liaison
DE102010064251A1 (de) 2010-12-28 2012-06-28 Robert Bosch Gmbh Handmessgerät zum Nachweis von verdeckten Schimmelschäden in Innenräumen
JP5754566B2 (ja) * 2011-08-26 2015-07-29 Ｊｆｅスチール株式会社 金属内部への侵入水素量の測定装置
JP5777098B2 (ja) * 2011-08-26 2015-09-09 Ｊｆｅスチール株式会社 金属内部への侵入水素量の測定方法および移動体の金属部位内部へ侵入する水素量のモニタリング方法
JP5700673B2 (ja) * 2011-08-26 2015-04-15 Ｊｆｅスチール株式会社 金属内部への侵入水素量の測定方法および移動体の金属部位内部へ侵入する水素量のモニタリング方法
CN103175872A (zh) * 2011-12-23 2013-06-26 长沙中生众捷生物技术有限公司 便携式电化学检测试纸条及其制备方法
KR101364647B1 (ko) 2012-03-14 2014-02-21 한국수력원자력 주식회사 고농도 비수용액 전해질에 적용가능한 금속이온 또는 산소이온의 모니터링 방법
DE102012205171B3 (de) 2012-03-29 2013-09-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Integriertes Einweg-Chipkartuschensystem für mobile Multiparameteranalysen chemischer und/oder biologischer Substanzen
GB201207869D0 (en) * 2012-05-04 2012-06-20 Accunostics Ltd Pulsed signal testing of biological fluid
EP3101415A1 (fr) * 2012-06-28 2016-12-07 Siemens Healthcare Diagnostics Inc. Dispositif de lecture et procédé d'amplification de signal
DE102012018539A1 (de) * 2012-09-19 2014-03-20 Seuffer Gmbh & Co.Kg Verfahren und Vorrichtung zur Erfassung von Eigenschaften fluider Medien
JP5979731B2 (ja) * 2014-01-08 2016-08-31 Ｊｆｅスチール株式会社 移動体の金属部位内部へ侵入する水素量のモニタリング方法
JP6621088B2 (ja) 2014-04-23 2019-12-18 テルモ株式会社 被分析物を測定するための非酵素的電気化学センサ
JP6130447B2 (ja) * 2015-07-29 2017-05-17 Ｊｆｅスチール株式会社 移動体の金属部位内部へ侵入する水素量のモニタリング方法
US11119101B2 (en) 2017-01-13 2021-09-14 Taiwan Semiconductor Manufacturing Co., Ltd. Cartridge and analyzer for fluid analysis

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04118554A (ja) * 1989-12-28 1992-04-20 Tosoh Corp 電気化学的酵素測定方法およびバイオセンサ
DE4318519C2 (de) 1993-06-03 1996-11-28 Fraunhofer Ges Forschung Elektrochemischer Sensor
DE19610115C2 (de) 1996-03-14 2000-11-23 Fraunhofer Ges Forschung Detektion von Molekülen und Molekülkomplexen
CA2300268A1 (fr) 1997-08-12 1999-02-18 Robert D. Macphee Systeme reporteur electrochimique permettant de detecter les dosages immunologiques analytiques et procedures de biologie moleculaire
DE19916867A1 (de) 1999-04-14 2000-10-19 Fraunhofer Ges Forschung Anordnung und Verfahren zur Herstellung planarer Arrays mit immobilisierten Molekülen
WO2001033206A1 (fr) 1999-11-02 2001-05-10 Advanced Sensor Technologies, Inc. Capteur amperometrique et potentiometrique combine de type microscopique
US7455975B2 (en) * 2000-04-14 2008-11-25 Esa Biosciences, Inc. Electrochemical detection of nucleic acid sequences
EP1409728A2 (fr) 2001-04-09 2004-04-21 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Analyse non enzymatique a liaison liposomique avec electrodes en reseau etroitement espacees (nel-ela) pour detecter et quantifier des acides nucleiques
WO2002082078A2 (fr) 2001-04-09 2002-10-17 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Systemes de detection a enzyme liee activee pour detection et quantification d'acides nucleiques, d'antigenes, d'anticorps et d'autres analytes
US8105478B2 (en) 2004-01-29 2012-01-31 Siemens Aktiengesellschaft Method for measuring the concentration or change in concentration of a redox-active substance and corresponding device
JP4007606B2 (ja) * 2004-02-03 2007-11-14 キヤノン株式会社 センサおよび検出方法
DE102005007539A1 (de) * 2005-02-17 2006-08-31 Universität Rostock Einrichtung zur Bestimmung redoxaktiver Stoffe
DE102005037436A1 (de) * 2005-08-04 2007-02-15 Siemens Ag Verfahren und System zur Konzentrationsbestimmung eines Analyt-Enzym-Komplexes oder Analyt-Enzym-Konjugats, insbesondere zur elektrochemischen Detektion des Analyten, und zugehörige Messvorrichtung
DE202005020141U1 (de) * 2005-11-29 2007-01-11 Farco-Pharma Gmbh Detektionseinheit, insbesondere Biosensor
DE102005062159A1 (de) 2005-11-29 2007-05-31 Farco-Pharma Gmbh Detektionseinheit, insbesondere Biosensor, und ihre Verwendung

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2009147093A1 *

Also Published As

Publication number Publication date
US20110156722A1 (en) 2011-06-30
DE102008027038A1 (de) 2009-12-17
WO2009147093A1 (fr) 2009-12-10
EP2286227B1 (fr) 2017-12-13
US8900440B2 (en) 2014-12-02
CN102057273B (zh) 2015-09-23
CN102057273A (zh) 2011-05-11
JP2011522266A (ja) 2011-07-28
JP5675594B2 (ja) 2015-02-25

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