Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F3/00—Biological treatment of water, waste water, or sewage
  • C02F3/02—Aerobic processes
  • C02F3/10—Packings; Fillings; Grids
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/20—Bacteria; Culture media therefor
  • C12N1/205—Bacterial isolates
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F1/00—Treatment of water, waste water, or sewage
  • C02F1/68—Treatment of water, waste water, or sewage by addition of specified substances, e.g. trace elements, for ameliorating potable water
  • C02F1/685—Devices for dosing the additives
  • C02F1/686—Devices for dosing liquid additives
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F2101/00—Nature of the contaminant
  • C02F2101/30—Organic compounds
  • C02F2101/301—Detergents, surfactants
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
  • C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
  • C02F2103/346—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from semiconductor processing, e.g. waste water from polishing of wafers
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F2209/00—Controlling or monitoring parameters in water treatment
  • C02F2209/06—Controlling or monitoring parameters in water treatment pH
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
  • C12R2001/00—Microorganisms ; Processes using microorganisms
  • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
  • Y02W10/00—Technologies for wastewater treatment
  • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

• the present invention relates to a process for the microbiological treatment of water, in particular waste water from semiconductor production, and microorganisms suitable for use in such a process. Furthermore, the invention relates to a bio-reactor and a water treatment plant with such microorganisms.
• Wastewater from semiconductor production generally has a better defined composition than fresh water, so it can be treated more specifically.
• Wastewater from semiconductor production contains no appreciable amounts of inorganic ingredients and hardness constituents (calcium or magnesium) or carbonates.
• Such wastewater has a total organic carbon (TOC) value in the range between 500 ppb and 3000 ppb.
• the organic ingredients are predominantly residues of synthetic organic solvents. These have a lower molecular weight compared to natural organic water constituents such as humic acids or biopolymers. Moreover, they are polar and practically indissociable. By way of example, 2-propanol can be cited, which often accounts for the largest proportion of the impurities.
• microorganisms are either suspended directly in the process water or applied to solid support materials, which are then brought into contact with the process water.
• the microorganisms absorb organic compounds from the wastewater as well as oxygen, nutrients and dissolved minerals and convert these impurities into inert or easily separable compounds.
• the organic carbon is preferably mineralized.
• the source of the microorganisms is generally sewage sludge, which is used to inoculate suitable reactors in the ultrapure water treatment process.
• a treatment with microorganisms can lead to a partially very significant reduction in the proportion of organic ingredients.
• the efficiency and reliability of such measures are often very volatile. The results obtained were not always reproducible.
• the present invention has for its object to provide a technical solution to the aforementioned problem.
• the solution should enable efficient and reproducible in particular the purification of waste water from the semiconductor industry with a high proportion of low molecular weight organic impurities such as 2-propanol and acetone. It should be simple and inexpensive.
• An inventive method is used for microbiological treatment of water, especially wastewater from semiconductor production. It comprises a treatment of the water to be treated in a bioreactor with microorganisms. Particular preference is given to inventing According to the treated water to be treated with microorganisms, of which at least 70% of 2-propanol and / or acetone can degrade.
• the microorganisms are thus specialized in the degradation of the compounds mentioned and not completely undefined.
• the microorganisms can be both a single bacterial strain and bacterial cultures which comprise several bacterial strains or even strains from different bacterial genera. It is crucial that the proportion of 2-propanol and / or acetone-degrading microorganisms does not fall below the mentioned limit.
• the proportion of 2-propanol and / or acetone-degrading microorganisms is preferably more than 80%, in particular more than 90%, particularly preferably almost 100%.
• microorganisms from sewage sludge for the degradation of low molecular weight organic compounds.
• this is by no means a targeted selection of microorganisms.
• the proportion of microorganisms which specifically break down certain impurities is determined rather by chance in all the procedures known hitherto.
• the water to be treated is particularly preferably treated with at least one bacterial strain of the bacterial genus Xanthobacter and / or a mutant thereof.
• this strain On standard media, this strain usually formed transparent, whitish, dull colonies with a smooth margin, round growth, homogeneous structure and flat profile. Diplococci with cokirkoid cell shape and rounded corners, a width of approx. 0.8 ⁇ m and a length of approx. 1.2 ⁇ m were observed.
• a precise phylogenetic assignment was carried out by determining the nucleic acid sequence by means of direct sequencing of the PCR-amplified 16S rRNA. This was followed by the comparison of the sequence data with the known sequences of representatives of the genus Xanthobacter. The highest match of the sequence of 99.5% was found to Xanthobacter flavus (DSM 338).
• the water to be treated according to the present invention may also be treated with at least one bacterial strain of the bacterial genus Rhodococcus and / or a mutant thereof.
• bacterial strains have also proved to be very suitable for use in a method according to the invention.
• DSMZ German Collection of Microorganisms and Cell Cultures GmbH
• the nucleic acid sequence was determined by direct sequencing of the PCR amplified 16S rRNA. By comparing the sequence data with the sequences of known representatives of the genus Rhodococcus, the strain could be identified as a representative of the suborder Rhodococcus ruber.
• bacterial strains of the bacterial genus Paracoccus and / or mutants thereof are very suitable for use in a method according to the invention.
• the bacterial strain which under the DMS no.
• DMSZ German Collection of Microorganisms and Cell Cultures GmbH
• the above bacterial strains identified as being preferred can be used both individually and in combination in a method according to the invention. They may also be present in mixed cultures with one or more other bacterial strains, which may not be able to degrade 2-propanol and / or acetone. However, the proportion of 2-propanol and / or acetone-degrading microorganisms is preferably always above the stated preferred limit values. In preferred embodiments of the process according to the invention, only one or more of the strains identified as being preferred are used as microorganisms.
• the above-mentioned bioreactor is understood here to mean a container in which the water to be treated is brought into contact with the microorganisms, for example by suspending the microorganisms in the water to be treated.
• the bioreactor used is particularly preferably a ventilated or vented filtration unit, which in preferred embodiments has a particulate filter material. So configured, the bioreactor can simultaneously perform several functions. On the one hand, low molecular weight organic ingredients such as 2-propanol and / or acetone can be degraded in it, on the other hand, it can act as a filter to separate particles from the water to be treated.
• the microorganisms used in a method according to the invention are immobilized on the filter material in a bioreactor designed as a filtration unit.
• the microorganisms can form a continuous film on the particles and / or in pores of the filter material.
• a bottom-up flow guide is generally indicated as the preferred mode of operation of biofilters (see, e.g., EP 585036, JP 7284799, JP 6063592 and JP 62065792).
• biofilters When flowing through a biofilter from bottom to top, however, microorganisms can easily be flushed out with the process water. As a result, downstream process stages can become contaminated. For this reason, bioreactors known from the prior art are often combined with additional filtration stages downstream of the biofilter (see, for example, JP 63185494, JP 8197094 and JP 9155371).
• such solutions are complex in terms of apparatus and expensive.
• the water to be treated is passed as a waste stream through such a bioreactor formed as a filtration unit. This has ensured that the filtration unit does not contain excessive concentrations of microorganisms Treatment be initiated directly into the filtrate water tank of a water treatment plant.
• a bioreactor according to the invention is used especially for water treatment, in particular for the treatment of wastewater from the semiconductor industry, and is particularly suitable for use in a method according to the invention. It has microorganisms that can degrade 2-propanol and / or acetone.
• bacterial strains of the bacterial genus Xanthobacter and / or Rhodococcus and / or Paracoccus or mutants thereof are particularly suitable as microorganisms.
• the above description of preferably usable microorganisms is hereby expressly referred to and incorporated by reference.
• a bioreactor according to the invention is in preferred embodiments designed as a ventilated or non-ventilated filtration unit, the filtration unit having a particulate filter material on which the microorganisms are immobilized.
• the advantageous bifunctionality of such a bioreactor has already been discussed above.
• the particulate filter material is particularly preferably activated carbon.
• other materials for example structured or random packings made of plastics such as polystyrene, sand, expanded clay or anthracite, may also be very suitable.
• the particulate filter material be porous, in particular pores having a size that exceeds the size of the microorganisms by at least a factor of 10.
• Activated carbon has a significant adsorption capacity for organic solvents, especially for 2-propanol and acetone. Loading the filter material with these solvents creates a supply of substrate to which the microbiological culture is specifically adapted. In operation, fluctuations in the quantity and composition of the wastewater to be treated can be compensated.
• the present invention further includes a water treatment plant, which is characterized in that it has a bioreactor according to the invention.
• Generic water treatment plants for the production of ultrapure water are well known from the prior art and therefore need not be described in detail in the context of this application.
• Microorganisms that are particularly suitable for use in a method of the invention or in a bioreactor of the invention are included in the present invention.
• a microorganism according to the invention is characterized in particular in that it comprises a bacterial strain of the bacterial genus Xanthobacter with the DSM no. 19987 or a mutant of it.
• Another microorganism according to the invention is characterized in that it contains a bacterial strain of the bacterial genus Rhodococcus with DSM no. 19985 or a mutant of it is.
• a third microorganism according to the invention belongs to the genus Paracoccus and was the DSMZ under the DSM no. Deposited in 19986.
• microorganisms according to the invention can be immobilized on a carrier material such as activated carbon. Other suitable carriers have been mentioned above as filter materials.
• microorganisms according to the invention may be packed in a watertight, air-permeable container.
• immobilized microorganisms according to the invention can also be preserved over extended periods of time, provided that they are packed in containers which ensure adequate oxygen supply of the microorganisms.
• containers are, for example flat bags made of plastic film, preferably made of polyethylene, in particular with a film thickness of about 0.05 mm. These have sufficient air permeability to ensure oxygenation of trapped microorganisms for extended periods of up to several weeks.
• the nutrient requirement of the microorganisms in this period can be covered, for example, by adsorptively stored 2-propanol on the carrier material.
• the bags can be sealed sterile and waterproof. In this way, the immobilized microorganisms can be stored for longer periods of time and transported over long distances to the place of use.
• the fixed bed of a filtration unit such as a fixed bed of activated carbon
• the immobilized microorganisms conversion of a simple filtration unit in a bioreactor according to the invention.
• An important prerequisite for the rapid proliferation of microorganisms in the bioreactor is the addition of nutrient salts to the reactor feed.
• the nutrient salts are preferably added until the bioreactor has reached its full capacity.
• the nutrient salts preferably contain the required macroelements (in particular N, P, S, Na, Ca and Mg) as well as trace elements (in particular Fe, Cu, Cr, Co and Zn).
• the microorganisms can be provided in consistent and controlled quality.
• FIG. 1 shows a flowchart of an embodiment of a subsection of a water treatment plant according to the invention for the treatment of reclaimed water.
• the water tank 2 has a means for regulating the pH of the reclaimed water contained.
• the pH in the water tank is set to values between 4 and 9, in particular between 7 and 8.
• From the water tank 2 leads a line 12 to the bioreactor 1, in which there is a fixed bed of activated carbon, on the surface microorganisms of the strain with the DSM no. 19985 are settled.
• the reclaim water can be mixed with electrolyte from the reservoir tank 3 via the line 16 if necessary. This may be necessary as the reclaim water may not be completely free of salt to ensure long term survival of the microorganisms in the bioreactor.
• the pre-tanks 4 and 5 contain 2-propanol and nutrient salts. From these, the Reclaimwasser via the lines 17 and 18 substrate (eg 2-propanol) and nutrients are added, which may be necessary in the activation phase or to compensate for fluctuations in concentration during operation. If necessary, the water to be treated can be circulated and ventilated. The latter may be necessary during startup for biological activation.
• the valves 6a and 6b are used for switching between FiuduküuiisueLiieb, circuit management and sudpüiung. The latter can eg be necessary if the fixed bed of the bioreactor is added with dirt particles from the water to be treated.
• Reference numbers 13 and 14 designate the process for water treated in the bioreactor in the production mode and the inlet of the backwashing stream.
• micro-organism referred to under I has been registered with the International Depositary on this date and the request for conversion of this initial deposit into a Budapest Treaty has been received (date of receipt of the application for conversion).

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• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Health & Medical Sciences (AREA)
• Organic Chemistry (AREA)
• Biotechnology (AREA)
• Zoology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Wood Science & Technology (AREA)
• Genetics & Genomics (AREA)
• Microbiology (AREA)
• Tropical Medicine & Parasitology (AREA)
• Biomedical Technology (AREA)
• Virology (AREA)
• Biochemistry (AREA)
• General Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Medicinal Chemistry (AREA)
• Biodiversity & Conservation Biology (AREA)
• Hydrology & Water Resources (AREA)
• Environmental & Geological Engineering (AREA)
• Water Supply & Treatment (AREA)
• Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
• Biological Treatment Of Waste Water (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)

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• 2008
  • 2008-02-06 DE DE200810009219 patent/DE102008009219A1/de not_active Withdrawn
• 2009
  • 2009-02-05 TW TW98103637A patent/TWI460137B/zh active
  • 2009-02-06 EP EP09708214A patent/EP2247540A2/de not_active Withdrawn
  • 2009-02-06 CN CN2009801120099A patent/CN102123955A/zh active Pending
  • 2009-02-06 WO PCT/EP2009/000824 patent/WO2009098066A2/de active Application Filing
  • 2009-02-06 US US12/866,560 patent/US20110186509A1/en not_active Abandoned

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