EP1590454A2 - Subtilases - Google Patents

Subtilases

Info

Publication number
EP1590454A2
EP1590454A2 EP04706617A EP04706617A EP1590454A2 EP 1590454 A2 EP1590454 A2 EP 1590454A2 EP 04706617 A EP04706617 A EP 04706617A EP 04706617 A EP04706617 A EP 04706617A EP 1590454 A2 EP1590454 A2 EP 1590454A2
Authority
EP
European Patent Office
Prior art keywords
atom
subtilase
seq
positions
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP04706617A
Other languages
German (de)
English (en)
Inventor
Allan Svendsen
Henriette Draborg
Nikolaj Tindbaek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to EP07118727A priority Critical patent/EP1914307A3/fr
Publication of EP1590454A2 publication Critical patent/EP1590454A2/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Definitions

  • subtilase or "(a) TY145 like subtilase” should in the context of the pre- sent invention be understood as a subtilase belonging to the Subtilisin group according to Siezen et al. Protein Science 6 (1997) 501-523 and which has at least 63% homology to TY145, SEQ ID NO:1. In the context of the present invention a TY145 subtilase has three ion-binding sites.
  • position is in the context of the present invention to be understood as the number of an amino acid in a peptide or polypeptide when counting from the N-terminal end of said peptide/polypeptide.
  • position numbers used in the present invention refer to different subtilases depending on which subgroup the subtilase belongs to.
  • subtilases belonging to the TY145 subgroup i.e. subtilases obtained from TY145, TA39, TA41 and Bacillus sphaericus are numbered individually according to each of SEQ ID NO:1 ,2,3 and 4.
  • the filter is then washed twice for 30 minutes in 2 x SSC, 0.5 % SDS at least * 55°C (low stringency), more preferably at least 60°C (medium stringency), still more preferably at least 65°C (medium/high stringency), even more preferably at least 70°C (high stringency), and even more preferably at least 75°C (very high stringency).
  • a BPN' subtilase is in the context of the present invention to be understood as a subtilase which has at least 61% homology to SEQ ID NO:5.
  • said BPN' subtilase may have at least 70%, such as at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to BPN', i.e. to SEQ ID NO:5.
  • subtilase consists of 6-8 helices, 11 strands of which 7 are central in a twisted beta-sheet. Two ion-binding sites are mentioned, one of which is the so called "Weak" calcium-binding site. It was later discovered that for some structures (subtilisin DY PDB no. 1 BH6, 1998), this calcium-binding site was shown to be a Na (sodium) binding site when the calcium concentration in the crystallization medium was low. Thus, in the following we refer to ion-binding sites instead of calcium-binding sites.
  • the structure of TY145 was solved in accordance with the principle for x-ray crystallographic methods, for example, as given in X-Ray Structure Determination, Stout, G.K. and Jensen, L.H., John Wiley & Sons, Inc. NY, 1989.
  • the structural coordinates for the solved crystal structure of TY145 are given in standard PDB format (Protein Data Bank, Brookhaven National Laboratory, Brookhaven, CT) as set forth in Appendix 1. It is to be understood that Appendix 1 forms part of the present application.
  • the present invention comprises a method of producing a variant of a parent TY145 like subtilase, the variant having at least one altered property as compared to the parent TY145 like subtilase, the method comprising: a) modelling the parent TY145 subtilase on the three-dimensional structure of a TY145 subtilase to produce a three-dimensional structure of the parent TY145 subtilase; b) comparing the three-dimensional structure obtained in step a) to the three-dimensional structure of a TY145 subtilase; c) identifying on the basis of the comparison in step b) at least one structural part of the parent TY145 subtilase, wherein an alteration in said structural part is predicted to result in an altered property; d) modifying the nucleic acid sequence encoding the parent TY145 subtilase to produce a nucleic acid sequence encoding deletion or substitution of one or more amino acids at a position corresponding to said structural part, or an insertion of one or more amino acid residues in positions
  • Removal of the Far site can be done by deletion(s) of or in the region K290-D300 (of SEQ ID NO:1) and subsequent insertion of one or more amino acid residues.
  • a preferred variant has the whole region deleted and a subsequent insertion between I289 and Y301 of the sequence GDS or DST.
  • the substitution S303Y is further added.
  • Removal of the Near site can be done by deletion(s) of or in the region N212-R224 (of SEQ ID NO:1) and subsequent insertion of one or more amino acid residues.
  • a preferred variant has the whole region deleted and a subsequent insertion of a proline or alanine residue between G211 and D225.
  • TY145 a mesophilic-derived enzyme obtained from crystal structure
  • TA41 a psychrophilic derived enzyme obtained from modelling
  • Regions closest to the active site and the substrate binding site are regarded as preferred in relation to making higher activity at low temperature for TY145: 40-73, and 140-161 , preferably 65-73 and 140-150.
  • the regions in TY145 should be modified to be more mobile for example by substitution with small less rigid residues, i.e. residues with smaller side chains (such as Gly, Ala, Ser, Thr or Val), into the TY145 backbone.
  • the substrate binding site is identified by the residues in contact with a substrate model, such as the CI2 inhibitor.
  • a substrate model such as the CI2 inhibitor.
  • the 3D structure coordinates of the TY145 subtilase with CI2 bound in the active site can be found in Appendix 1. Without being limited to any theory, it is presently believed that binding between a substrate and an enzyme is supported by favor- able interactions found within a sphere 10 A from the substrate molecule, in particular within a sphere of 6 A from the substrate molecule. Examples of such favorable bonds are hydrogen bonds, strong electrostatic interaction and/or hydrophobic interactions.
  • Asn-Gly sequences can be found in the following positions: B. sphaericus: 198-199, 240-241 TY145: 87-88, 109-110, 199-200 TA41 : 83-84, 198-199 TA39: 88-89, 198-199
  • the present invention in this respect thus relates to modifications, such as deletions and substitutions in one or more of these positions in accordance with the principles given above.
  • subtilase variant described herein may advantageously be com- bined with one or more modification(s) in any of the positions:
  • the host cell into which the DNA construct or the recombinant vector of the invention is introduced may be any cell that is capable of producing the present subtilase variants, such as prokaryotes, e.g. bacteria or eukaryotes, such as fungal cells, e.g. yeasts or filamentous fungi, insect cells, plant cells or mammalian cells.
  • prokaryotes e.g. bacteria or eukaryotes
  • fungal cells e.g. yeasts or filamentous fungi
  • insect cells e.g. yeasts or filamentous fungi
  • plant cells or mammalian cells examples of bacterial host cells which, on cultivation, are capable of producing the subtilase variants of the invention are gram-positive bacteria such as strains of Bacillus, e.g. strains of B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B.
  • Examples of useful proteases are the variants described in WO 92/19729, WO 98/20115, WO 98/20116, and WO 98/34946, especially the variants with substitutions in one or more of the following positions: 27, 36, 57, 76, 87, 97, 101 , 104, 120, 123, 167, 170, 194, 206, 218, 222, 224, 235 and 274.
  • cellulases are the alkaline or neutral cellulases having colour care benefits.
  • Examples of such cellulases are cellulases described in EP 0 495 257, EP 0 531 372, WO 96/11262, WO 96/29397, WO 98/08940.
  • Other examples are cellulase variants such as 5 those described in WO 94/07998, EP 0 531 315, US 5,457,046, US 5,686,593, US 5,763,254, WO 95/24471 , WO 98/12307 and PCT/DK98/00299.
  • the detergent may contain a bleaching system which may comprise a H 2 0 2 source such as perborate or percarbonate which may be combined with a peracid-forming bleach activator such as tetraacetylethylenediamine or nonanoyloxybenzenesulfonate.
  • a bleaching system may comprise peroxyacids of e.g. the amide, imide, or sulfone type.
  • AMSA wash tests were performed on variants with Segment X and Segments X and XI like described in Example 5 herein.
  • the assay was conducted under the experimental conditions specified below:
  • HCIC Hydrophobic Charge Induction Chromatography
  • 4-MEP 4-Mercapto-Ethyl-Pyridine
  • Beads of the cellulose matrix sized 80-100 ⁇ m are mixed with a media containing yeast and the transformed B. subtilis capable of secreting the subtilisin variants and incubated at pH 9.5 in Unifilter ® microplates.
  • ATOM 422 C ILE A 29 28, .143 24 .586 9. .459 1, .00 14. .30 c
  • ATOM 507 N ASP A 35 21, .571 9, .765 12. .490 1. .00 11, .34 N
  • ATOM 604 CA HIS A 42 12 .245 17 .917 1, .419 1, .00 21, .28 C
  • ATOM 1069 CA HIS A 75 12 .345 15 .653 11, ,803 1. .00 13, .53 C

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés pour produire des variantes d'une subtilase TY145 apparentée et d'une subtilase BPN' apparentée, ainsi que des variantes de TY145 et de BPN' présentant des propriétés modifiées par rapport à la subtilase TY145/BPN' apparentée.
EP04706617A 2003-01-30 2004-01-30 Subtilases Ceased EP1590454A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07118727A EP1914307A3 (fr) 2003-01-30 2004-01-30 Substitulases

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DK200300119 2003-01-30
DKPA200300119 2003-01-30
US44530003P 2003-02-05 2003-02-05
US445300P 2003-02-05
DKPA200300689 2003-05-07
DK200300689 2003-05-07
PCT/DK2004/000066 WO2004067737A2 (fr) 2003-01-30 2004-01-30 Subtilases

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP07118727A Division EP1914307A3 (fr) 2003-01-30 2004-01-30 Substitulases

Publications (1)

Publication Number Publication Date
EP1590454A2 true EP1590454A2 (fr) 2005-11-02

Family

ID=32830227

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04706617A Ceased EP1590454A2 (fr) 2003-01-30 2004-01-30 Subtilases

Country Status (2)

Country Link
EP (1) EP1590454A2 (fr)
WO (1) WO2004067737A2 (fr)

Families Citing this family (164)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7727756B2 (en) 2003-03-21 2010-06-01 Novozymes A/S Subtilases
DE602005026541D1 (de) * 2004-09-21 2011-04-07 Novozymes As Subtilasen
WO2006067198A2 (fr) * 2004-12-22 2006-06-29 Direvo Biotech Ag Nouvelles entites biologiques et utilisation ciblee de celles-ci
DE102007032111B4 (de) * 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) * 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
CN101649301B (zh) * 2009-09-08 2012-07-25 迟仁立 一种微生物菌液和使用其制备生物有机肥的方法
CN102758378B (zh) * 2011-04-28 2015-09-09 徐奎元 一种旧棉改造再生利用的方法
WO2013076269A1 (fr) 2011-11-25 2013-05-30 Novozymes A/S Variants de subtilase et polynucléotides codants pour ceux-ci
CN105358686A (zh) 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
CN105358685A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
EP3309249B1 (fr) 2013-07-29 2019-09-18 Novozymes A/S Variants de protéases et polynuclétides les codant
EP2832853A1 (fr) 2013-07-29 2015-02-04 Henkel AG&Co. KGAA Composition détergente comprenant des variantes de protéases
US10030239B2 (en) 2013-12-20 2018-07-24 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
US11407989B2 (en) 2014-10-30 2022-08-09 Novozymes A/S Protease variants and polynucleotides encoding same
EP3212785A2 (fr) * 2014-10-30 2017-09-06 Novozymes A/S Variants de protéase et polynucléotides les codant
US10400230B2 (en) 2014-12-19 2019-09-03 Novozymes A/S Protease variants and polynucleotides encoding same
WO2016097357A1 (fr) * 2014-12-19 2016-06-23 Novozymes A/S Variants de protéase et polynucléotides les codant
CN107002059A (zh) 2014-12-19 2017-08-01 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
US11518987B2 (en) 2014-12-19 2022-12-06 Novozymes A/S Protease variants and polynucleotides encoding same
EP3253858A1 (fr) * 2015-02-04 2017-12-13 Novozymes A/S Composition de détergent comprenant des variants de protéase et d'amylase
EP3280791A1 (fr) 2015-04-10 2018-02-14 Novozymes A/S Procédé de lavage de linge, utilisation d'adnase et composition détergente
CN107636134A (zh) 2015-04-10 2018-01-26 诺维信公司 洗涤剂组合物
US20180171318A1 (en) 2015-10-14 2018-06-21 Novozymes A/S Polypeptides Having Protease Activity and Polynucleotides Encoding Same
BR112018008454B1 (pt) 2015-10-28 2023-09-26 Novozymes A/S Composição detergente que compreende variantes de amilase e protease, seu uso e métodos para lavar
EP3380608A1 (fr) 2015-11-24 2018-10-03 Novozymes A/S Polypeptides ayant une activité de protéase et polynucléotides codant pour ceux-ci
CA3007170A1 (fr) 2015-12-29 2017-07-06 Novozymes A/S Compositions detergentes et utilisations de celles-ci
BR112018069220A2 (pt) 2016-03-23 2019-01-22 Novozymes As uso de polipeptídeo que tem atividade de dnase para tratamento de tecidos
BR112018072282A2 (pt) 2016-04-29 2019-02-12 Novozymes A/S composições detergentes e usos das mesmas
EP3464538A1 (fr) 2016-05-31 2019-04-10 Novozymes A/S Compositions stabilisées de peroxyde liquide
WO2017220422A1 (fr) 2016-06-23 2017-12-28 Novozymes A/S Utilisation d'enzymes, composition et procédé d'élimination de salissures
WO2018002261A1 (fr) 2016-07-01 2018-01-04 Novozymes A/S Compositions détergentes
WO2018007573A1 (fr) 2016-07-08 2018-01-11 Novozymes A/S Compositions détergentes contenant de la galactanase
JP6858850B2 (ja) 2016-07-13 2021-04-14 ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ バチルス・シビ（ＢＡＣＩＬＬＵＳ ＣＩＢＩ）ＤＮａｓｅ変異体及びその使用
EP3275989A1 (fr) 2016-07-26 2018-01-31 The Procter and Gamble Company Composition de détergent de lave-vaisselle automatique
EP3275985A1 (fr) 2016-07-26 2018-01-31 The Procter and Gamble Company Composition de détergent de lave-vaisselle automatique
EP3275988B1 (fr) 2016-07-26 2020-07-08 The Procter and Gamble Company Composition de détergent de lave-vaisselle automatique
EP3275987A1 (fr) 2016-07-26 2018-01-31 The Procter and Gamble Company Composition de détergent de lave-vaisselle automatique
EP3275986B1 (fr) 2016-07-26 2020-07-08 The Procter and Gamble Company Composition de détergent de lave-vaisselle automatique
EP3519547A1 (fr) 2016-09-29 2019-08-07 Novozymes A/S Granule contenant des spores
EP3519548A1 (fr) 2016-09-29 2019-08-07 Novozymes A/S Utilisation d'enzyme pour le lavage, procédé de lavage et composition pour laver la vaisselle
US20210284933A1 (en) 2016-10-25 2021-09-16 Novozymes A/S Detergent compositions
WO2018102478A1 (fr) 2016-12-02 2018-06-07 The Procter & Gamble Company Compositions de nettoyage comprenant des enzymes
EP3330349B1 (fr) 2016-12-02 2024-06-19 The Procter & Gamble Company Compositions de nettoyage comprenant des enzymes
EP3551740B1 (fr) 2016-12-12 2021-08-11 Novozymes A/S Utilisation de polypeptides
EP3339423A1 (fr) 2016-12-22 2018-06-27 The Procter & Gamble Company Composition de détergent de lave-vaisselle automatique
US11053483B2 (en) 2017-03-31 2021-07-06 Novozymes A/S Polypeptides having DNase activity
EP3601551A1 (fr) 2017-03-31 2020-02-05 Novozymes A/S Polypeptides présentant une activité de rnase
US11208639B2 (en) 2017-03-31 2021-12-28 Novozymes A/S Polypeptides having DNase activity
CN114480034A (zh) 2017-04-04 2022-05-13 诺维信公司 糖基水解酶
US20200109352A1 (en) 2017-04-04 2020-04-09 Novozymes A/S Polypeptide compositions and uses thereof
EP3607039A1 (fr) 2017-04-04 2020-02-12 Novozymes A/S Polypeptides
CN111108183A (zh) 2017-06-30 2020-05-05 诺维信公司 酶浆液组合物
MX2020002953A (es) 2017-09-20 2020-07-22 Novozymes As Uso de enzimas para mejorar la absorcion de agua y/o la blancura.
US11414814B2 (en) 2017-09-22 2022-08-16 Novozymes A/S Polypeptides
CN111247245A (zh) 2017-10-27 2020-06-05 宝洁公司 包含多肽变体的洗涤剂组合物
BR112020008251A2 (pt) 2017-10-27 2020-11-17 Novozymes A/S variantes de dnase
DE102017125560A1 (de) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa Reinigungszusammensetzungen, die dispersine iii enthalten
DE102017125558A1 (de) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa Reinigungszusammensetzungen, die dispersine i enthalten
DE102017125559A1 (de) 2017-11-01 2019-05-02 Henkel Ag & Co. Kgaa Reinigungszusammensetzungen, die dispersine ii enthalten
US11505767B2 (en) 2017-11-01 2022-11-22 Novozymes A/S Methods for cleansing medical devices
WO2019086528A1 (fr) 2017-11-01 2019-05-09 Novozymes A/S Polypeptides et compositions comprenant de tels polypeptides
EP4379029A1 (fr) 2017-11-01 2024-06-05 Novozymes A/S Polypeptides et compositions comprenant de tels polypeptides
EP3502245A1 (fr) 2017-12-19 2019-06-26 The Procter & Gamble Company Composition de détergent de lave-vaisselle automatique
EP3502227B1 (fr) 2017-12-19 2024-09-04 The Procter & Gamble Company Composition de détergent de lave-vaisselle automatique
EP3502246A1 (fr) 2017-12-19 2019-06-26 The Procter & Gamble Company Composition de détergent de lave-vaisselle automatique
EP3502244A1 (fr) 2017-12-19 2019-06-26 The Procter & Gamble Company Composition de détergent de lave-vaisselle automatique
JP2021512986A (ja) 2018-02-28 2021-05-20 ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ 洗浄方法
CN111801409A (zh) 2018-02-28 2020-10-20 宝洁公司 清洁组合物
CN112262207B (zh) 2018-04-17 2024-01-23 诺维信公司 洗涤剂组合物中包含碳水化合物结合活性的多肽及其在减少纺织品或织物中的褶皱的用途
EP3810769A1 (fr) 2018-06-19 2021-04-28 The Procter & Gamble Company Composition de détergent pour lave-vaisselle automatique
EP3810768A1 (fr) 2018-06-19 2021-04-28 The Procter & Gamble Company Composition de détergent pour lave-vaisselle automatique
US12012573B2 (en) 2018-07-02 2024-06-18 Novozymes A/S Cleaning compositions and uses thereof
WO2020007875A1 (fr) 2018-07-03 2020-01-09 Novozymes A/S Compositions de nettoyage et leurs utilisations
WO2020008024A1 (fr) 2018-07-06 2020-01-09 Novozymes A/S Compositions de nettoyage et leurs utilisations
CN112969775A (zh) 2018-10-02 2021-06-15 诺维信公司 清洁组合物
EP3861110A1 (fr) 2018-10-02 2021-08-11 Novozymes A/S Ribonucléases d'endonucléase 1 pour le nettoyage
WO2020070014A1 (fr) 2018-10-02 2020-04-09 Novozymes A/S Composition de nettoyage comprenant un tensioactif anionique et un polypeptide ayant une activité rnase
WO2020070209A1 (fr) 2018-10-02 2020-04-09 Novozymes A/S Composition de nettoyage
WO2020070199A1 (fr) 2018-10-03 2020-04-09 Novozymes A/S Polypeptides ayant une activité de dégradation de l'alpha-mannane et polynucléotides codant pour ceux-ci
US20220033739A1 (en) 2018-10-11 2022-02-03 Novozymes A/S Cleaning compositions and uses thereof
EP3647398B1 (fr) 2018-10-31 2024-05-15 Henkel AG & Co. KGaA Compositions de nettoyage contenant des dispersines v
EP3647397A1 (fr) 2018-10-31 2020-05-06 Henkel AG & Co. KGaA Compositions de nettoyage contenant des dispersions iv
US20220017844A1 (en) 2018-12-03 2022-01-20 Novozymes A/S Low pH Powder Detergent Composition
CN113366103A (zh) 2018-12-21 2021-09-07 诺维信公司 具有肽聚糖降解活性的多肽以及编码其的多核苷酸
CN113330101A (zh) 2018-12-21 2021-08-31 诺维信公司 包含金属蛋白酶的洗涤剂袋
MX2021011104A (es) 2019-03-14 2021-10-22 Procter & Gamble Composiciones de limpieza que comprenden enzimas.
EP3938503A1 (fr) 2019-03-14 2022-01-19 The Procter & Gamble Company Procédé de traitement du coton
CN113439116B (zh) 2019-03-14 2023-11-28 宝洁公司 包含酶的清洁组合物
AU2020242303A1 (en) 2019-03-21 2021-06-24 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3947668A4 (fr) * 2019-04-02 2023-04-19 Novozymes A/S Compositions de détergent liquide pour laver la vaisselle
MX2021011981A (es) 2019-04-03 2021-11-03 Novozymes As Polipeptidos que tienen actividad de beta-glucanasa, polinucleotidos que los codifican y usos de los mismos en composiciones de limpieza y de detergente.
WO2020207944A1 (fr) 2019-04-10 2020-10-15 Novozymes A/S Variants polypeptidiques
EP3741283A1 (fr) 2019-05-22 2020-11-25 The Procter & Gamble Company Procédé de lavage automatique de la vaisselle
EP3976775A1 (fr) 2019-05-24 2022-04-06 The Procter & Gamble Company Composition détergente pour lave-vaisselle automatique
EP3997202A1 (fr) 2019-07-12 2022-05-18 Novozymes A/S Émulsions enzymatiques pour détergents
WO2021030400A1 (fr) 2019-08-13 2021-02-18 Novozymes Bioag A/S Combinaisons pesticides de yersinia et de protéases
CN114787329A (zh) 2019-08-27 2022-07-22 诺维信公司 洗涤剂组合物
CN114616312A (zh) 2019-09-19 2022-06-10 诺维信公司 洗涤剂组合物
US20220340843A1 (en) 2019-10-03 2022-10-27 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
US11492571B2 (en) 2019-10-24 2022-11-08 The Procter & Gamble Company Automatic dishwashing detergent composition comprising a protease
US20210122998A1 (en) 2019-10-24 2021-04-29 The Procter & Gamble Company Automatic dishwashing detergent composition comprising an amylase
EP3835396A1 (fr) 2019-12-09 2021-06-16 The Procter & Gamble Company Composition de détergent comportant un polymère
US20230048546A1 (en) 2019-12-20 2023-02-16 Henkel Ag & Co. Kgaa Cleaning compositions comprising dispersins vi
CN114929848A (zh) 2019-12-20 2022-08-19 诺维信公司 稳定的液体无硼酶组合物
WO2021122117A1 (fr) 2019-12-20 2021-06-24 Henkel Ag & Co. Kgaa Composition de nettoyage comprenant une dispersine et une carbohydrase
AU2020405786A1 (en) 2019-12-20 2022-08-11 Henkel Ag & Co. Kgaa Cleaning compositions comprising dispersins IX
EP4077656A2 (fr) 2019-12-20 2022-10-26 Novozymes A/S Polypeptides présentant une activité protéolytique et leur utilisation
EP3862412A1 (fr) 2020-02-04 2021-08-11 The Procter & Gamble Company Composition de détergent
EP3892708A1 (fr) 2020-04-06 2021-10-13 Henkel AG & Co. KGaA Compositions de nettoyage comprenant des variantes de dispersine
US20230167384A1 (en) 2020-04-21 2023-06-01 Novozymes A/S Cleaning compositions comprising polypeptides having fructan degrading activity
MX2022013878A (es) 2020-06-05 2022-11-30 Procter & Gamble Composiciones detergentes que contienen un surfactante ramificado.
EP3936593A1 (fr) 2020-07-08 2022-01-12 Henkel AG & Co. KGaA Compositions de nettoyage et leurs utilisations
WO2022031309A1 (fr) 2020-08-04 2022-02-10 The Procter & Gamble Company Procédé de lavage automatique de la vaisselle
WO2022031311A1 (fr) 2020-08-04 2022-02-10 The Procter & Gamble Company Procédé de lavage automatique de la vaisselle
JP2023536081A (ja) 2020-08-04 2023-08-23 ザ プロクター アンド ギャンブル カンパニー 自動食器洗浄方法
JP2023537336A (ja) 2020-08-04 2023-08-31 ザ プロクター アンド ギャンブル カンパニー 自動食器洗浄方法及びパック
CN116507725A (zh) 2020-10-07 2023-07-28 诺维信公司 α-淀粉酶变体
EP4232539A2 (fr) 2020-10-20 2023-08-30 Novozymes A/S Utilisation de polypeptides ayant une activité de dnase
MX2023004260A (es) 2020-10-29 2023-04-25 Procter & Gamble Composiciones de limpieza que contienen enzimas alginato liasas.
EP4001388A1 (fr) 2020-11-17 2022-05-25 The Procter & Gamble Company Procédé de lavage automatique de la vaisselle avec un polymère greffé amphiphile dans le rinçage
WO2022108766A1 (fr) 2020-11-17 2022-05-27 The Procter & Gamble Company Composition pour lave-vaisselle comprenant un polymère greffé amphiphile
CA3197110A1 (fr) 2020-11-17 2022-05-27 Patrick Firmin August Delplancke Procede de lavage automatique de la vaisselle presentant un rincage alcalin
EP4006131A1 (fr) 2020-11-30 2022-06-01 The Procter & Gamble Company Méthode de lavage de tissu
JP2023551014A (ja) 2020-12-23 2023-12-06 ビーエーエスエフ ソシエタス・ヨーロピア 両親媒性アルコキシル化ポリアミン及びそれらの使用
EP4032966A1 (fr) 2021-01-22 2022-07-27 Novozymes A/S Composition enzymatique liquide avec piégeur de sulfite
CN117015592A (zh) 2021-02-12 2023-11-07 诺维信公司 稳定的生物洗涤剂
EP4291646A2 (fr) 2021-02-12 2023-12-20 Novozymes A/S Variants d'alpha-amylase
EP4305146A1 (fr) 2021-03-12 2024-01-17 Novozymes A/S Variants polypeptidiques
EP4060036A1 (fr) 2021-03-15 2022-09-21 Novozymes A/S Variantes de polypeptides
CA3199985A1 (fr) 2021-03-15 2022-09-22 Lars Lehmann Hylling Christensen Compositions de nettoyage contenant des variants polypeptidiques
WO2022194673A1 (fr) 2021-03-15 2022-09-22 Novozymes A/S Variants de la dnase
EP4095223A1 (fr) 2021-05-05 2022-11-30 The Procter & Gamble Company Procédés de fabrication de compositions de nettoyage et de détection de salissures
EP4086330A1 (fr) 2021-05-06 2022-11-09 The Procter & Gamble Company Traitement de surface
EP4108767A1 (fr) 2021-06-22 2022-12-28 The Procter & Gamble Company Compositions de nettoyage ou de traitement contenant des enzymes nucléases
WO2022268885A1 (fr) 2021-06-23 2022-12-29 Novozymes A/S Polypeptides d'alpha-amylase
EP4123006A1 (fr) 2021-07-19 2023-01-25 The Procter & Gamble Company Composition comprenant des spores et des matériaux de pro-parfum
EP4123007A1 (fr) 2021-07-19 2023-01-25 The Procter & Gamble Company Traitement de tissu à l'aide de spores bactériennes
WO2023064749A1 (fr) 2021-10-14 2023-04-20 The Procter & Gamble Company Tissu et produit d'entretien domestique comprenant un polymère cationique facilitant le lavage et une enzyme lipase
EP4194537A1 (fr) 2021-12-08 2023-06-14 The Procter & Gamble Company Cartouche de traitement du linge
EP4194536A1 (fr) 2021-12-08 2023-06-14 The Procter & Gamble Company Cartouche de traitement du linge
EP4448712A1 (fr) 2021-12-16 2024-10-23 The Procter & Gamble Company Composition pour lave-vaisselle automatique comprenant une protéase
US20230272310A1 (en) 2021-12-16 2023-08-31 The Procter & Gamble Company Home care composition
CA3240638A1 (fr) 2021-12-16 2023-06-22 Michelle Jackson Composition de soin textile et menager comprenant une protease
CN118369413A (zh) 2021-12-16 2024-07-19 宝洁公司 包含淀粉酶的家庭护理组合物
EP4206309A1 (fr) 2021-12-30 2023-07-05 Novozymes A/S Particules de protéines à blancheur améliorée
AU2023210344A1 (en) * 2022-01-21 2024-06-13 Novozymes A/S Cleaning method, use of enzymes and cleaning composition
WO2023165507A1 (fr) 2022-03-02 2023-09-07 Novozymes A/S Utilisation de xyloglucanase pour l'amélioration de la durabilité de détergents
AU2023228020A1 (en) 2022-03-04 2024-07-11 Novozymes A/S Dnase variants and compositions
WO2023194204A1 (fr) 2022-04-08 2023-10-12 Novozymes A/S Variants et compositions d'hexosaminidase
EP4273210A1 (fr) 2022-05-04 2023-11-08 The Procter & Gamble Company Compositions détergentes contenant des enzymes
EP4273209A1 (fr) 2022-05-04 2023-11-08 The Procter & Gamble Company Compositions pour le nettoyage des machines contenant des enzymes
EP4279571A1 (fr) 2022-05-19 2023-11-22 The Procter & Gamble Company Composition de lavage comprenant des spores
EP4321604A1 (fr) 2022-08-08 2024-02-14 The Procter & Gamble Company Tissu et composition de soins à domicile comprenant un tensioactif et un polyester
WO2024094802A1 (fr) 2022-11-04 2024-05-10 The Procter & Gamble Company Tissu et composition d'entretien ménager
WO2024094790A1 (fr) 2022-11-04 2024-05-10 Clariant International Ltd Polyesters
WO2024094803A1 (fr) 2022-11-04 2024-05-10 The Procter & Gamble Company Composition d'entretien textile et ménager
WO2024119298A1 (fr) 2022-12-05 2024-06-13 The Procter & Gamble Company Composition de soin textile et ménager comprenant un composé de carbonate de polyalkylène
WO2024129520A1 (fr) 2022-12-12 2024-06-20 The Procter & Gamble Company Composition de soin textile et ménager
EP4386074A1 (fr) 2022-12-16 2024-06-19 The Procter & Gamble Company Composition de soin pour le linge et le domicile
EP4388967A1 (fr) 2022-12-19 2024-06-26 The Procter & Gamble Company Procédé de lavage de vaisselle
WO2024131880A2 (fr) 2022-12-23 2024-06-27 Novozymes A/S Composition détergente comprenant une catalase et une amylase
WO2024156628A1 (fr) 2023-01-23 2024-08-02 Novozymes A/S Compositions de nettoyage et leurs utilisations
EP4410941A1 (fr) 2023-02-01 2024-08-07 The Procter & Gamble Company Compositions détergentes contenant des enzymes
WO2024194245A1 (fr) 2023-03-21 2024-09-26 Novozymes A/S Compositions détergentes à base de biotensioactifs

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001291649A1 (en) * 2000-10-02 2002-04-15 Novozymes A/S Nucleic acids encoding polypeptides having proteolytic activity

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2004067737A2 *

Also Published As

Publication number Publication date
WO2004067737A3 (fr) 2004-12-02
WO2004067737A2 (fr) 2004-08-12

Similar Documents

Publication Publication Date Title
US10144922B2 (en) Subtilase variants and compositions comprising the same
WO2004067737A2 (fr) Subtilases
US8563289B2 (en) Protease variants
US8008057B2 (en) Subtilases
US7507569B2 (en) Variant subtilisin enzymes (subtilases)
JP2012045000A (ja) サブチラーゼ
EP2013339B1 (fr) Variants de savinase offrant une meilleure efficacité de lavage contre les taches d'oeuf
US20040147008A1 (en) Subtilase variants
US6777218B1 (en) Subtilase enzymes having an improved wash performance on egg stains
CN1768137A (zh) 枯草杆菌酶
EP1914307A2 (fr) Substitulases
EP1313862A1 (fr) Procede de production d'un complexe inhibiteur de proteases
WO2005010176A1 (fr) Procedes pour fabriquer des variants de polypeptides par echange de fragments combinatoires

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20050830

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK

DAX Request for extension of the european patent (deleted)
17Q First examination report despatched

Effective date: 20060426

17Q First examination report despatched

Effective date: 20060426

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED

18R Application refused

Effective date: 20080615