EP1587552A2 - Procede et dispositif de desinfection d'un cryostat de microtome - Google Patents

Procede et dispositif de desinfection d'un cryostat de microtome

Info

Publication number
EP1587552A2
EP1587552A2 EP03767660A EP03767660A EP1587552A2 EP 1587552 A2 EP1587552 A2 EP 1587552A2 EP 03767660 A EP03767660 A EP 03767660A EP 03767660 A EP03767660 A EP 03767660A EP 1587552 A2 EP1587552 A2 EP 1587552A2
Authority
EP
European Patent Office
Prior art keywords
disinfectant
cryostat
microtome
exposure time
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP03767660A
Other languages
German (de)
English (en)
Other versions
EP1587552B1 (fr
Inventor
Dieter Teppke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microm International GmbH
Original Assignee
Microm International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microm International GmbH filed Critical Microm International GmbH
Publication of EP1587552A2 publication Critical patent/EP1587552A2/fr
Application granted granted Critical
Publication of EP1587552B1 publication Critical patent/EP1587552B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/20—Gaseous substances, e.g. vapours
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/24—Apparatus using programmed or automatic operation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome

Definitions

  • the invention relates to a method for disinfecting a microtome cryostat with a defrosting phase, providing a vaporous disinfectant for acting on the closed cryostat chamber and an exposure time for the disinfectant.
  • the invention further relates to a device for disinfecting a microtome cryostat with a microtome in a cryostat chamber and a refrigeration device, and with a device for providing a vaporous disinfectant and a controller which is designed such that it provides this and an exposure time in Following a defrosting phase.
  • tissue samples that are infected with germs are often cut with microtomes, disinfection is necessary to protect the operator. Spraying is particularly problematic with cryostat microtomes, since the moisture must be removed from the K-ryostat chamber again. If moisture remains on a cryostat microtome, it freezes after cooling for the next operation. This can freeze guides and other things, making the microtome unusable. Or the disinfectant remains liquid in the chamber, for example in the case of highly concentrated alcoholic solutions, and leads to further impairments up to the risk of explosion.
  • the invention is therefore based on the object of developing a method and a device of the type mentioned at the outset such that rapid and effective disinfection and the fastest possible restart of a microtome cryostat with safe drying is possible.
  • the object is achieved according to the invention in that after the exposure time in the cryostat chamber, a temperature difference is generated and the disinfectant which is deposited in the colder area is removed.
  • the object of the invention is achieved in that the control is designed such that it causes the development of a temperature difference in the cryostat chamber after heating and / or cooling and a collecting device for removing dejected disinfectant is arranged in the colder area.
  • agents can be used as disinfectants.
  • examples include glutaraldehyde, benzalkonium chloride, formalin and didecyldimethylammonium chloride.
  • the temperature difference can be achieved both by cooling an area and by heating an area. It is advisable to use existing cooling or heating devices. It can be provided that after the exposure time the temperature of the refrigeration device of the cryostat is lowered in a cooling phase below 0 ° C until at least a large part of the disinfectant has deposited on the refrigeration device, and that the refrigeration device is then thawed to remove the disinfectant by means of a Remove the collecting device from the cryostat chamber. Also, such a collecting device is normally arranged under the refrigeration unit, so that the melt water of defrosted frost or ice can be removed from the cryostat chamber. It is also possible that the microtome is heated up after the exposure time.
  • Such heating heats the disinfectant, for example the disinfectant solution, out of the microtome and to colder parts of the device, which means that the microtome is in the dry state for the subsequent cooling phase.
  • the temperature difference can of course be achieved both by cooling and by heating, but the combination of the two aforementioned measures is the most expedient.
  • the colder areas within the cryostat chamber are most appropriately selected in such a way that precipitating disinfectant or moisture can be well collected and drained off.
  • the heating goes well above the ambient temperature of the cryostat.
  • the vaporous disinfectant be blown into the cryostat chamber, this forced convection leading to uniform wetting of all components.
  • Evaporation of the disinfectant can be effected by heating, but preferably evaporation is carried out by means of ultrasound, since this enables a uniform aerosol to be generated without the phases of substances in the disinfectant being separated. This serves a uniform disinfection effect.
  • the disinfectant can of course also be present in vapor form.
  • the cryostat is expediently warmed up to at least the ambient temperature after the defrosting phase. This warming-up is then expediently followed by a temperature equalization time so that all components are at the same temperature and are therefore subjected to uniform disinfection during the exposure time of the disinfectant.
  • Various heaters can be used for heating, expediently one in Microtome built-in heating is used. The heating via the microtome prevents it from thawing and thus dilution of the solvent during the actual subsequent disinfection during the contact time, so that a perfect disinfection of the microtome can be achieved.
  • Mechanical cutting waste should be removed before the vapor disinfectant is made available. This can be done manually or it can be provided that the cut waste is suctioned off. This prevents contamination from remaining in the cryostat chamber via the cut waste.
  • vaporous disinfectant is sucked into a suction system in order to disinfect this too.
  • Suction disinfects hoses, filters, pumps and shut-off devices in the suction system.
  • control is designed such that it lowers the temperature of the cryostat's refrigeration generator after the exposure time in a cooling phase below 0 ° C until at least a large part of the disinfectant has precipitated on the refrigeration generator and the refrigeration generator then defrosted is used to remove the disinfectant from the cryostat chamber by means of the collecting device.
  • the microtome has a heater and the controller is set up in such a way that it causes the microtome to heat up after the exposure time.
  • the refrigeration generator also has a heater, the controller being designed such that it switches the heater on to accelerate the defrosting.
  • the device for providing a vaporous disinfectant expediently has a blower which introduces the vaporous disinfectant into the cryostat chamber.
  • a forced confection in the cryostat chamber can thus be achieved with an even distribution of the disinfectant.
  • the device for evaporating the disinfectant is expediently equipped with an ultrasound actuator in order to achieve the above-mentioned atomization of the disinfectant without phase separation.
  • the device for producing the vaporous disinfectant is expediently supplied with disinfectant by means of a storage container.
  • a valve can ensure that the liquid level of the disinfectant can be regulated in the device for producing the vaporous disinfectant.
  • Fig. 1 is an exemplary representation of the temperature profiles and process steps of an inventive method
  • Fig. 2 shows an embodiment of an inventive microtome cryostat.
  • FIG. 1 shows an example of possible temperature profiles of the method according to the invention, the temperatures T being plotted in ° C. against the time t and possible method steps 11 to 19 being entered.
  • a defrosting phase 11 is initiated and a warming-up 12 to at least ambient temperature Tu, which is approximately in a range from 20 to 25 ° C.
  • Tu ambient temperature
  • This process is expediently accelerated in that the heater 7 of the microtome 6 is operated, the heating via the microtome 6 having the advantage that it is not condensed. Then it cannot happen during the subsequent disinfection that the disinfectant 2 is diluted by this dew.
  • FIG. 2 With regard to the device features, reference is made to FIG. 2.
  • the temperature T M of the latter rises faster than the temperature T K of the cryostat chamber 3. It is therefore expedient for the warming-up 12 to be followed by a temperature compensation time 12 '.
  • a vaporous disinfectant 2 is provided 13 by blowing it into the cryostat chamber 3.
  • an exposure time 14 is waited until heating 15 of the microtome 6 is then initiated by the heater 7 of the microtome 6.
  • the temperature TM of the microtome 6 rises further, for example to 50 ° C. This serves to transfer moisture and disinfectant solution out of the microtome 6 to the colder parts of the cryostat chamber 3.
  • the latter is cooled in a cooling phase 16 below 0 ° C., preferably in the range of -10 ° C.
  • This defrosting 17 of the refrigeration generator 4 is expediently supported by a heater 10 of the refrigeration generator 4, which is heated, for example, to + 6 ° C. If the defrosting liquid is removed and the cryostat chamber 3 and especially the microtome 6 are dry, cooling can be carried out again for restarting 19 so that the microtome cryostat 1 again reaches its operating temperature for renewed object processing.
  • the microtome cryostat 1 consists of a cooled housing which encloses a cryostat chamber 3 and is revealed by means of an opening 32.
  • a microtome 6 with an object holder 33 and a knife 34 for making the cuts.
  • the cryostat chamber 3 is cooled by means of a refrigeration generator 4, for example an evaporator, which is connected to a coolant supply 30.
  • Both the microtome 6 and the cold generator 4 are equipped with heaters 7 and 10 in order to be able to carry out rapid defrosting and heating.
  • a collecting device 5 for example in the form of a drip tray, which discharges thawing liquid out of the cryostat chamber 3 into a storage container 28 via an outlet 25.
  • a device 8 for providing a vaporous disinfectant 2, which can blow this vaporized disinfectant 2 into the cryostat chamber 3 via a pipe in the direction of the arrows 35.
  • a blower 20 is provided and an ultrasonic actuator 21 for generating the aerosol from the liquid disinfectant 2.
  • a storage container 22 and a valve 23 serve to ensure that the liquid level 24 of the disinfectant 2 is always maintained in the device 8.
  • a controller 9 is provided, which is connected to the heater 7 of the microtome 6, to the heater 10 of the refrigeration generator 4, to the ultrasound actuator 21, the blower 20 and the coolant supply 30 of the refrigeration generator 4 is connected. Connection lines 31 are used for this purpose.
  • the controller 9 can also be connected to sensors (not shown) for detecting temperatures, air humidity, etc., in order to be able to effectively regulate the temperatures and drying described.
  • a connection to a fan 29 of an extraction system 26 and to the valve 23 and further functional elements is possible.
  • controller 9 can control the temperature profiles T K , T M and Tv shown in FIG. 1 and method steps 11 to 19 by controlling the heaters 7 and / or 10 and the cooling by means of the cooling generator 4. In addition, it controls the provision 13 of the vaporous disinfectant 2 via the device 8.
  • controller 9 can ensure that vaporous disinfectant 2 is sucked into the suction system 26 in order to disinfect the fan 29, the pipes and the filter 27 there, for example.
  • the representation of the temperature profiles T K , T M and Tv and the work steps 11 to 19 of FIG. 1 as well as the device features of FIG. 2 is only exemplary.
  • the temperature differences ⁇ Ti and ⁇ T 2 for the precipitation of disinfectant 2 and moisture and their removal are essential.
  • An alternative configuration of heaters or an alternative generation of temperature differences within the cryostat chamber 3 would also be possible, for example only by heating or only by cooling, or a different time sequence can be selected in which, for example, method steps 11 to 19 can partially overlap.
  • Microtome cryostat (cryostat) disinfectant cryostat chamber refrigeration (of the cryostat), z.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un procédé de désinfection d'un cryostat de microtome (1), consistant à réaliser une phase de dégivrage (11), à se munir (13) d'un agent désinfectant vaporeux (2) destiné à agir sur la chambre cryostatique fermée (3), et à faire agir (14) l'agent désinfectant (2). L'invention concerne également un dispositif destiné à la mise en oeuvre dudit procédé. L'invention vise à améliorer lesdits procédé et dispositif afin de permettre une désinfection rapide et efficace, ainsi qu'une remise en service rapide du cryostat de microtome (1), tout en garantissant un séchage efficace. A cet effet, une différence de température (<T1, ?T2) est créée dans la chambre cryostatique (3) après la durée d'action (14), et l'agent désinfectant (2) déposé par précipitation dans une zone plus froide est évacué.
EP03767660A 2003-02-01 2003-11-26 Cryostat de microtome et procede de sa desinfection Expired - Lifetime EP1587552B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10303989A DE10303989B4 (de) 2003-02-01 2003-02-01 Verfahren und Vorrichtung zur Desinfektion eines Mikrotom-Kryostaten
DE10303989 2003-02-01
PCT/EP2003/013267 WO2004067047A2 (fr) 2003-02-01 2003-11-26 Procede et dispositif de desinfection d'un cryostat de microtome

Publications (2)

Publication Number Publication Date
EP1587552A2 true EP1587552A2 (fr) 2005-10-26
EP1587552B1 EP1587552B1 (fr) 2011-08-17

Family

ID=32747522

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03767660A Expired - Lifetime EP1587552B1 (fr) 2003-02-01 2003-11-26 Cryostat de microtome et procede de sa desinfection

Country Status (6)

Country Link
US (2) US20060133950A1 (fr)
EP (1) EP1587552B1 (fr)
JP (1) JP4210654B2 (fr)
AU (1) AU2003292118A1 (fr)
DE (1) DE10303989B4 (fr)
WO (1) WO2004067047A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7319230B2 (en) * 2004-07-15 2008-01-15 Skaggs Donald E Disinfection and decontamination using ultraviolet light
JP4840763B2 (ja) * 2006-01-18 2011-12-21 セイコーインスツル株式会社 自動薄切片作製装置及び自動薄切片標本作製装置
DE102009030624B4 (de) 2009-06-25 2012-06-21 Microm International Gmbh Verfahren und Vorrichtung zur Desinfektion eines Mikrotom-Kryostaten
DE102012106846B4 (de) * 2012-07-27 2014-09-25 Leica Biosystems Nussloch Gmbh Mikrotom mit einem Piezolinearantrieb
US11073447B2 (en) * 2016-03-31 2021-07-27 Agilent Technologies, Inc. Apparatus and methods for transferring a tissue section
CN113786510B (zh) * 2021-09-18 2023-04-21 沛县盛玛特新材料研究院有限公司 一种医用棉球处理设备

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Publication number Priority date Publication date Assignee Title
DE3202627A1 (de) * 1982-01-25 1983-08-11 Melagapparate Gmbh, 1000 Berlin Verfahren zum abfuehren eines sterilisiermediums aus einem sterilisierapparat und vorrichtung zur durchfuehrung des verfahrens
US4909999A (en) * 1987-07-06 1990-03-20 American Sterilizer Company Flow-through vapor phase sterilization system
US4952370A (en) * 1988-05-06 1990-08-28 American Sterilizer Company Hydrogen peroxide sterilization method
US5122344A (en) * 1990-01-31 1992-06-16 Mdt Corporation Chemical sterilizer system
FR2705587A1 (fr) * 1993-05-25 1994-12-02 Tabone Herve Cryostat de microtomie, notamment pour travaux histologiques.
US5711705A (en) * 1995-05-25 1998-01-27 Flanders Filters, Inc. Isolation work station
US6030579A (en) * 1996-04-04 2000-02-29 Johnson & Johnson Medical, Inc. Method of sterilization using pretreatment with hydrogen peroxide
DE19803966B4 (de) * 1997-03-07 2006-05-11 Microm International Gmbh Absaugeinrichtung für Schnittabfälle bei einem Kryostat-Mikrotom und Kryostat-Mikrotom
AUPP427398A0 (en) * 1998-06-23 1998-07-16 Novapharm Research (Australia) Pty Ltd Improved disinfection
GB2354443A (en) * 1999-09-21 2001-03-28 Microflow Ltd Vapour phase sterilisation
US6481219B2 (en) * 2001-03-30 2002-11-19 Sakura Finetek U.S.A., Inc. Disinfection system and method of using same

Non-Patent Citations (1)

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Title
See references of WO2004067047A2 *

Also Published As

Publication number Publication date
WO2004067047A2 (fr) 2004-08-12
WO2004067047A3 (fr) 2004-10-21
AU2003292118A1 (en) 2004-08-23
EP1587552B1 (fr) 2011-08-17
JP4210654B2 (ja) 2009-01-21
US20080181814A1 (en) 2008-07-31
US20060133950A1 (en) 2006-06-22
AU2003292118A8 (en) 2004-08-23
US8246905B2 (en) 2012-08-21
DE10303989B4 (de) 2006-07-06
JP2006513749A (ja) 2006-04-27
DE10303989A1 (de) 2004-08-26

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