EP1579014A2 - Procede permettant de deceler des bacteries du genre legionella - Google Patents
Procede permettant de deceler des bacteries du genre legionellaInfo
- Publication number
- EP1579014A2 EP1579014A2 EP04762665A EP04762665A EP1579014A2 EP 1579014 A2 EP1579014 A2 EP 1579014A2 EP 04762665 A EP04762665 A EP 04762665A EP 04762665 A EP04762665 A EP 04762665A EP 1579014 A2 EP1579014 A2 EP 1579014A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- detection
- legionella
- probe
- probes
- species
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 52
- 208000007764 Legionnaires' Disease Diseases 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000894006 Bacteria Species 0.000 title abstract 3
- 239000000523 sample Substances 0.000 claims abstract description 83
- 241000589248 Legionella Species 0.000 claims abstract description 38
- 238000009396 hybridization Methods 0.000 claims abstract description 29
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract description 27
- 241000894007 species Species 0.000 claims abstract description 16
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000011895 specific detection Methods 0.000 claims abstract description 4
- 241000589242 Legionella pneumophila Species 0.000 claims description 7
- 229940115932 legionella pneumophila Drugs 0.000 claims description 7
- 241000589276 Legionella jordanis Species 0.000 claims description 5
- 230000014670 detection of bacterium Effects 0.000 claims description 5
- 238000000338 in vitro Methods 0.000 description 10
- 239000012528 membrane Substances 0.000 description 10
- 239000000872 buffer Substances 0.000 description 9
- 108020004465 16S ribosomal RNA Proteins 0.000 description 6
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 6
- 239000002751 oligonucleotide probe Substances 0.000 description 6
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 5
- 230000027455 binding Effects 0.000 description 5
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 5
- 239000011976 maleic acid Substances 0.000 description 5
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 2
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical group C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 210000003705 ribosome Anatomy 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- BMYCCWYAFNPAQC-UHFFFAOYSA-N 2-[dodecyl(methyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCN(C)CC(O)=O BMYCCWYAFNPAQC-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 206010001889 Alveolitis Diseases 0.000 description 1
- 241000224489 Amoeba Species 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 241000937819 Legionella pneumophila serogroup 1 Species 0.000 description 1
- 208000004023 Legionellosis Diseases 0.000 description 1
- 208000000112 Myalgia Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 108020001027 Ribosomal DNA Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 241000589262 Tatlockia micdadei Species 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- UKWLRLAKGMZXJC-QIECWBMSSA-L disodium;[4-chloro-3-[(3r,5s)-1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl]phenyl] phosphate Chemical compound [Na+].[Na+].O1OC2([C@@H]3CC4C[C@H]2CC(Cl)(C4)C3)C1(OC)C1=CC(OP([O-])([O-])=O)=CC=C1Cl UKWLRLAKGMZXJC-QIECWBMSSA-L 0.000 description 1
- 201000001155 extrinsic allergic alveolitis Diseases 0.000 description 1
- 208000021760 high fever Diseases 0.000 description 1
- 208000022098 hypersensitivity pneumonitis Diseases 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 244000000056 intracellular parasite Species 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 208000030247 mild fever Diseases 0.000 description 1
- 208000013465 muscle pain Diseases 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- Figure 1 p.8 shows a schematic representation of the sand wipe hybridization method.
- the capture probe 1 is labeled with biotin 4; binds to magnetic beads 7 coated with streptavidin 7.
- detection is carried out with alkaline phosphatase 6 which is attached to the digoxigenin labeled detection probe 2 binds via Anti DIG Fab fragments.
- the detection of the amplified fluorescence signal can be quantified by a fluorescence reader. Another possibility of detection is the detection of the activity of the alkaline phosphatase by electrochemical sensors.
- the 16S ribosomal DNA was amplified from the total DNA of various Legionella species using the fD1 and rP2 universal primers for the 16SrDNA by means of PCR.
- the promoter region of the T7 polymerase was contained in the fD1.
- 166Sr RNA transcribed in vitro was prepared from the corresponding PCR products from Legionella (16SrDN A) using the DIG RNA LabelingKit (SP6 / T7) (Röche) or the MAXIscript-Kit / Ambion.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un procédé permettant de déceler des bactéries du genre Legionella par des procédés d'hybridation en « sandwich » à des températures de 50-55 °C. Selon l'invention, on utilise comme sondes de captage et de détection, à cet effet, des oligonucléotides récemment développés et dont les séquences permettent l'identification des bactéries du genre Legionella de manière spécifique au genre ou au type. L'interchangeabilité mutuelle des sondes de captage et de détection lors de détections, spécifiques au genre et au type, du genre Legionella. En outre, ce procédé de détection peut être mis en oeuvre avec des combinaisons d'oligonucléotides pour des sondes spécifiques au type.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10338123 | 2003-08-15 | ||
| DE2003138123 DE10338123B3 (de) | 2003-08-15 | 2003-08-15 | Verfahren zum Nachweis von Bakterien der Gattung Legionella |
| PCT/DE2004/001821 WO2005017194A2 (fr) | 2003-08-15 | 2004-08-13 | Procede permettant de deceler des bacteries du genre legionella |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1579014A2 true EP1579014A2 (fr) | 2005-09-28 |
Family
ID=34177691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP04762665A Withdrawn EP1579014A2 (fr) | 2003-08-15 | 2004-08-13 | Procede permettant de deceler des bacteries du genre legionella |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20070184437A1 (fr) |
| EP (1) | EP1579014A2 (fr) |
| DE (2) | DE10338123B3 (fr) |
| WO (1) | WO2005017194A2 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8609829B2 (en) * | 2005-10-17 | 2013-12-17 | Gen-Probe Incorporated | Compositions and methods to detect Legionella pneumophila nucleic acid |
| WO2007047912A2 (fr) * | 2005-10-17 | 2007-04-26 | Gen-Probe Incorporated | Compositions et procedes destines a detecter des acides nucleiques de legionella pneumophila |
| EP1852512B1 (fr) * | 2006-05-02 | 2011-11-02 | Universite Pierre Et Marie Curie | Méthode pour la détection et l'énumération de microorganismes |
| EP2202305A4 (fr) * | 2007-10-04 | 2011-01-19 | Tosoh Corp | Amorce pour l'amplification de l'arnr d'une bactérie appartenant à l'espèce<i>legionella</i>, procédé de détection et coffret de détection |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988003957A1 (fr) * | 1986-11-24 | 1988-06-02 | Gen-Probe Incorporated | Sondes d'acide nucleique pour la detection et/ou la quantification d'organismes non viraux |
| WO1993020234A1 (fr) * | 1992-03-31 | 1993-10-14 | E.I. Du Pont De Nemours And Company | Dosage rapide, a haute capacite, fonde sur l'acide nucleique |
| WO1994028174A1 (fr) * | 1993-05-24 | 1994-12-08 | Amoco Corporation | Sondes d'acides nucleiques destinees a des bacteries du genre legionella |
| DE19515891C2 (de) * | 1995-04-29 | 1999-10-28 | Roche Diagnostics Gmbh | Gattungs- und speziesspezifische Identifizierung von Legionellen |
-
2003
- 2003-08-15 DE DE2003138123 patent/DE10338123B3/de not_active Expired - Fee Related
-
2004
- 2004-08-13 WO PCT/DE2004/001821 patent/WO2005017194A2/fr not_active Ceased
- 2004-08-13 US US10/554,238 patent/US20070184437A1/en not_active Abandoned
- 2004-08-13 EP EP04762665A patent/EP1579014A2/fr not_active Withdrawn
- 2004-08-13 DE DE112004002047T patent/DE112004002047D2/de not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| See references of WO2005017194A2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| DE112004002047D2 (de) | 2006-08-10 |
| US20070184437A1 (en) | 2007-08-09 |
| DE10338123B3 (de) | 2005-07-28 |
| WO2005017194A3 (fr) | 2005-07-21 |
| WO2005017194A2 (fr) | 2005-02-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR |
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| AX | Request for extension of the european patent |
Extension state: AL HR LT LV MK |
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| 17P | Request for examination filed |
Effective date: 20060117 |
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| DAX | Request for extension of the european patent (deleted) | ||
| RBV | Designated contracting states (corrected) |
Designated state(s): AT CH DE FI GB LI |
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