EP1579014A2 - Procede permettant de deceler des bacteries du genre legionella - Google Patents

Procede permettant de deceler des bacteries du genre legionella

Info

Publication number
EP1579014A2
EP1579014A2 EP04762665A EP04762665A EP1579014A2 EP 1579014 A2 EP1579014 A2 EP 1579014A2 EP 04762665 A EP04762665 A EP 04762665A EP 04762665 A EP04762665 A EP 04762665A EP 1579014 A2 EP1579014 A2 EP 1579014A2
Authority
EP
European Patent Office
Prior art keywords
detection
legionella
probe
probes
species
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04762665A
Other languages
German (de)
English (en)
Inventor
Antje Breitenstein
Tarja Rosilainen
Peter Neubauer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scanbec GmbH
Original Assignee
Scanbec GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scanbec GmbH filed Critical Scanbec GmbH
Publication of EP1579014A2 publication Critical patent/EP1579014A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • Figure 1 p.8 shows a schematic representation of the sand wipe hybridization method.
  • the capture probe 1 is labeled with biotin 4; binds to magnetic beads 7 coated with streptavidin 7.
  • detection is carried out with alkaline phosphatase 6 which is attached to the digoxigenin labeled detection probe 2 binds via Anti DIG Fab fragments.
  • the detection of the amplified fluorescence signal can be quantified by a fluorescence reader. Another possibility of detection is the detection of the activity of the alkaline phosphatase by electrochemical sensors.
  • the 16S ribosomal DNA was amplified from the total DNA of various Legionella species using the fD1 and rP2 universal primers for the 16SrDNA by means of PCR.
  • the promoter region of the T7 polymerase was contained in the fD1.
  • 166Sr RNA transcribed in vitro was prepared from the corresponding PCR products from Legionella (16SrDN A) using the DIG RNA LabelingKit (SP6 / T7) (Röche) or the MAXIscript-Kit / Ambion.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant de déceler des bactéries du genre Legionella par des procédés d'hybridation en « sandwich » à des températures de 50-55 °C. Selon l'invention, on utilise comme sondes de captage et de détection, à cet effet, des oligonucléotides récemment développés et dont les séquences permettent l'identification des bactéries du genre Legionella de manière spécifique au genre ou au type. L'interchangeabilité mutuelle des sondes de captage et de détection lors de détections, spécifiques au genre et au type, du genre Legionella. En outre, ce procédé de détection peut être mis en oeuvre avec des combinaisons d'oligonucléotides pour des sondes spécifiques au type.
EP04762665A 2003-08-15 2004-08-13 Procede permettant de deceler des bacteries du genre legionella Withdrawn EP1579014A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10338123 2003-08-15
DE2003138123 DE10338123B3 (de) 2003-08-15 2003-08-15 Verfahren zum Nachweis von Bakterien der Gattung Legionella
PCT/DE2004/001821 WO2005017194A2 (fr) 2003-08-15 2004-08-13 Procede permettant de deceler des bacteries du genre legionella

Publications (1)

Publication Number Publication Date
EP1579014A2 true EP1579014A2 (fr) 2005-09-28

Family

ID=34177691

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04762665A Withdrawn EP1579014A2 (fr) 2003-08-15 2004-08-13 Procede permettant de deceler des bacteries du genre legionella

Country Status (4)

Country Link
US (1) US20070184437A1 (fr)
EP (1) EP1579014A2 (fr)
DE (2) DE10338123B3 (fr)
WO (1) WO2005017194A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8609829B2 (en) 2005-10-17 2013-12-17 Gen-Probe Incorporated Compositions and methods to detect Legionella pneumophila nucleic acid
AU2006304721B2 (en) * 2005-10-17 2012-01-19 Gen-Probe Incorporated Compositions and methods to detect Legionella pneumophila nucleic acid
EP1852512B1 (fr) * 2006-05-02 2011-11-02 Universite Pierre Et Marie Curie Méthode pour la détection et l'énumération de microorganismes
EP2202305A4 (fr) * 2007-10-04 2011-01-19 Tosoh Corp Amorce pour l'amplification de l'arnr d'une bactérie appartenant à l'espèce<i>legionella</i>, procédé de détection et coffret de détection

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU616646B2 (en) * 1986-11-24 1991-11-07 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
WO1993020234A1 (fr) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company Dosage rapide, a haute capacite, fonde sur l'acide nucleique
WO1994028174A1 (fr) * 1993-05-24 1994-12-08 Amoco Corporation Sondes d'acides nucleiques destinees a des bacteries du genre legionella
DE19515891C2 (de) * 1995-04-29 1999-10-28 Roche Diagnostics Gmbh Gattungs- und speziesspezifische Identifizierung von Legionellen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2005017194A2 *

Also Published As

Publication number Publication date
WO2005017194A2 (fr) 2005-02-24
DE112004002047D2 (de) 2006-08-10
US20070184437A1 (en) 2007-08-09
WO2005017194A3 (fr) 2005-07-21
DE10338123B3 (de) 2005-07-28

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