EP1502946B1 - Solid State Fermenter - Google Patents
Solid State Fermenter Download PDFInfo
- Publication number
- EP1502946B1 EP1502946B1 EP04018255A EP04018255A EP1502946B1 EP 1502946 B1 EP1502946 B1 EP 1502946B1 EP 04018255 A EP04018255 A EP 04018255A EP 04018255 A EP04018255 A EP 04018255A EP 1502946 B1 EP1502946 B1 EP 1502946B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- fermenter
- solid
- cooling
- state
- seal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007787 solid Substances 0.000 title abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 33
- 239000000758 substrate Substances 0.000 claims abstract description 15
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 239000002826 coolant Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 2
- 229920001296 polysiloxane Polymers 0.000 claims description 2
- 125000006850 spacer group Chemical group 0.000 claims description 2
- 239000003779 heat-resistant material Substances 0.000 claims 1
- 230000000630 rising effect Effects 0.000 claims 1
- 238000000855 fermentation Methods 0.000 abstract description 7
- 230000004151 fermentation Effects 0.000 abstract description 7
- 238000011081 inoculation Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000110 cooling liquid Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
Definitions
- the invention relates to a solid-state fermenter for the cultivation of microorganisms on solid substrates, especially for large volumes.
- Field of application of the invention is the microbiological industry.
- WO 99/57239 already a solid-state fermenter is known, which consists of at least two air and water permeable, one above the other arranged Fermenteretagen that are connected to the wall of the container that neither air nor water can flow past the side, wherein on the Fermenteretagen a culture substrate located for the microorganisms to be cultivated and under each floor a cooling device is mounted
- the invention therefore an object of the said disadvantages of in WO 99/57239 claimed solid state fermenter by design changes to eliminate and allow the use of large volumes.
- the cooling of the fermentation product is carried out by vertically extending from the fermenter bottom cooling lances ( Fig. 1/3 ) guaranteed.
- the distance of the cooling lances is determined by the amount of heat produced by the cultured microorganisms.
- the cooling lances are in a triangular composite ( Fig. 2 ) are arranged to each other so that each cooling lance has the same distance to the nearest one.
- the cooling lances consist of an inner tube of smaller diameter, which serves for the return of the coolant, and a tube of larger diameter, in which the first is centrally located, which serves the flow of the coolant ( Fig. 3 ).
- the cooling lance is a circular ring.
- the outer tube is provided at the upper end with a circular cone-shaped closure.
- the cooling lances have an outer diameter of preferably 1 - 3 cm.
- the ratio of the diameters of the two tubes to each other should preferably be designed so that the flow velocity of the Coolant in the flow equal to the flow velocity of the coolant in the return is.
- the cooling lances are below the lowest fermenter floor as in Fig. 1 represented connected with 2 in the fermenter in the leading tubes, one of which serves for flow and the other to the return of the cooling liquid.
- the outer wall of the fermenter which is designed as a pressure vessel, for the purpose of cooling the fermenter good only at 2 points to be broken.
- the bottoms of the fermenter floors are provided with holes.
- the holes have a diameter that is about 1 mm larger than the outer diameter of the cooling lancets. They can be provided with lids which open upwards, ie into the culture substrate. When introducing the Fermenteretagen from the top of the fermenter these covers are pushed open by the cooling lances and thus give the way for the same free. Due to the circular cone-shaped tip, the cooling lancets can easily penetrate the culture substrate during the introduction of the fermenter stages into the fermenter. Due to the volume occupied by the cooling lancets, a fermenter floor may only be filled with culture substrate before it is introduced into the fermenter so that, after it has been introduced into the fermenter, it still has sufficient space in the fermenter floor.
- the filling of the Fermenteretagen can also be done after the relevant floor has already been introduced into the fermenter, namely such that the floor is just below the fermenter opening and the cooling lances have penetrated the fermentor floor to the intended height of the culture substrate.
- no lid for closing the holes in the fermenter floors are required. The floors are filled to the intended height and placed in the fermenter until they rest on the underlying fermenter floor.
- the fermenter described can be operated with only one filled with the culture substrate floor. This is particularly possible if the granular culture substrate used has a very stable structure and therefore there is no danger that the culture substrate during the Sterilization or fermentation condensed or changed its properties in a different way to the detriment of the cultural process.
- the inoculum is then introduced, which is distributed in the water.
- a uniform inoculation of all Fermenteretagen is subsequently achieved in that the water is drained by a Bodenabfluß again from the fermenter.
- all fermenter floors are flowed through evenly and at the same time contaminated with the inoculum.
- this is only possible if the water does not flow past the side of the fermenter floors.
- the air- and watertight seal is achieved according to the invention by a seal which is installed between the fermenter floors and is compressed by the mass of the respective upper floor ( Fig. 1/6 and 1 / 6a ).
- the seal consists of an elastic, heat-stable material (eg silicone). By compressing the gasket, it expands sideways and is pressed against the inner wall of the fermenter. As a result, the necessary air and watertight seal is guaranteed.
- the upper fermenter floor is located on the lower one. This can be z. B. by spacers ( Fig. 1 / 6a ), through which the space for the compressed seal between all floors is the same size.
- the seal can be installed either at the upper edge of the lower floor or at the edge under the floor of the upper floor, for example by means of a groove.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Processing Of Solid Wastes (AREA)
- Fertilizers (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL04018255T PL1502946T3 (pl) | 2003-07-31 | 2004-08-02 | Fermentor z podłożem stałym |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10335522 | 2003-07-31 | ||
DE10335522A DE10335522A1 (de) | 2003-07-31 | 2003-07-31 | Solid-State-Fermenter |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1502946A1 EP1502946A1 (de) | 2005-02-02 |
EP1502946B1 true EP1502946B1 (de) | 2009-02-04 |
Family
ID=33521538
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP04018255A Expired - Lifetime EP1502946B1 (de) | 2003-07-31 | 2004-08-02 | Solid State Fermenter |
Country Status (17)
Country | Link |
---|---|
US (1) | US7476534B2 (es) |
EP (1) | EP1502946B1 (es) |
JP (1) | JP4663640B2 (es) |
CN (1) | CN100347283C (es) |
AT (1) | ATE422210T1 (es) |
AU (1) | AU2004261682B2 (es) |
BR (1) | BRPI0409837B1 (es) |
CA (1) | CA2520721C (es) |
CO (1) | CO5660305A2 (es) |
DE (2) | DE10335522A1 (es) |
DK (1) | DK1502946T3 (es) |
MX (1) | MXPA05011688A (es) |
PL (1) | PL1502946T3 (es) |
RU (1) | RU2359026C2 (es) |
TR (1) | TR200504027T2 (es) |
WO (1) | WO2005012478A1 (es) |
ZA (1) | ZA200508807B (es) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL1025609C2 (nl) * | 2004-03-01 | 2005-09-05 | Buehler Gmbh | Inrichting voor het weken van gerst. |
EP2186876A1 (en) * | 2008-11-13 | 2010-05-19 | Biorecycling Group B.V. | System for solid state fermentation and use thereof |
CN101768546B (zh) * | 2010-02-24 | 2013-01-23 | 中国科学院过程工程研究所 | 一种中药蠕动式固态发酵技术及装置 |
DE102010010293B4 (de) | 2010-03-04 | 2014-01-09 | fzmb GmbH, Forschungszentrum für Medizintechnik und Biotechnologie | Festphasenfermenter |
CN102234205B (zh) * | 2010-04-23 | 2013-06-05 | 中国科学院过程工程研究所 | 一种多功能固态发酵反应器 |
CN102250755A (zh) * | 2011-06-20 | 2011-11-23 | 娄底市裕德科技有限公司 | 一种多功能间歇膨胀固态发酵罐 |
CO6780221A1 (es) * | 2012-04-17 | 2013-10-31 | Univ De Caldas | Biorreactor para la obtención de sustancias bioactivas por fermentación en estado sólido empleando hongos macromicetos |
BR112014030587A2 (pt) * | 2012-06-06 | 2018-04-24 | Novozymes Bioag As | biorreator para meio sólido em escala de produção com um vaso do reator, e, sistema de biorreator para meio sólido em escala de produção. |
CN103789194B (zh) * | 2013-10-12 | 2016-03-16 | 云南龙谷生物科技有限公司 | 一种微生物培养罐 |
CN103695293B (zh) * | 2013-12-20 | 2015-12-09 | 中粮生物化学(安徽)股份有限公司 | 一种使用微生物固态培养装置进行微生物固态培养的方法 |
US20150322394A1 (en) * | 2014-05-06 | 2015-11-12 | City University Of Hong Kong | System and method of producing glucomylases and/or proteases |
EP3034600A1 (en) | 2014-12-16 | 2016-06-22 | Bayer CropScience Biologics GmbH | Method for prolonging the viability of fungal spores in liquid formulations |
AU2015366109B2 (en) * | 2014-12-19 | 2018-05-17 | Q Power Oy | Bioreactor and fermentation process for producing hydrogen |
CN105349413A (zh) * | 2015-11-03 | 2016-02-24 | 湖北民族学院 | 一种立式固体发酵系统 |
US20170218316A1 (en) * | 2016-01-29 | 2017-08-03 | Thomas K. Blake | Novel System for Germinating and Malting Grain |
DK3475408T3 (da) * | 2016-06-23 | 2021-08-23 | Q Power Oy | Faststoffermenteringsreaktor, som er udstyret med aktivt bærermateriale |
EP3400801A1 (en) | 2017-05-10 | 2018-11-14 | Bayer CropScience Aktiengesellschaft | Plant health effect of purpureocillium lilacinum |
RU2647762C1 (ru) * | 2017-04-20 | 2018-03-19 | Сергей Евгеньевич Угловский | Установка для выработки тепловой энергии |
CN111373027A (zh) | 2017-09-20 | 2020-07-03 | 拜耳作物科学生物制品有限责任公司 | 改善真菌孢子的储存稳定性和适应性的方法 |
EP3806639A1 (en) | 2018-06-15 | 2021-04-21 | Bayer Aktiengesellschaft | Liquid and storage-stable formulations for fungal spores |
JP2021525545A (ja) | 2018-07-10 | 2021-09-27 | バイエル・アクチエンゲゼルシヤフト | 液体真菌胞子製剤用の新規担体流体 |
US20220007643A1 (en) | 2018-07-20 | 2022-01-13 | Bayer Aktiengesellschaft | Novel carrier fluids for liquid fungal spore formulations |
CA3112957A1 (en) | 2018-09-19 | 2020-03-26 | Bayer Cropscience Biologics Gmbh | Method for increasing storage stability of fungal spores |
CA3161379A1 (en) * | 2019-12-13 | 2021-06-17 | Sean Farmer | Reactor for two-stage liquid-solid state fermentation of microorganisms |
US20230212502A1 (en) | 2020-05-28 | 2023-07-06 | Danstar Ferment Ag | Novel fermentation substrate for solid-state fermentation |
WO2021249972A1 (en) | 2020-06-08 | 2021-12-16 | Bayer Cropscience Biologics Gmbh | Novel formulations for increasing the germination rate of fungal spores |
WO2022040510A1 (en) | 2020-08-21 | 2022-02-24 | Bayer Cropscience Lp | Combinations of trichoderma and bradyrhizobium |
CN114369518A (zh) * | 2022-01-26 | 2022-04-19 | 贺州酸状元食品科技有限公司 | 一种酸菜发酵系统 |
CN114505103B (zh) * | 2022-03-23 | 2024-03-22 | 河北森斯环保科技有限公司 | 一种固态发酵酱酒中游离酸去除装置 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH469806A (fr) * | 1966-12-01 | 1969-03-15 | Afico Sa | Procédé de fermentation et fermenteur pour la mise en oeuvre de ce procédé |
JPS61197900U (es) * | 1985-05-28 | 1986-12-10 | ||
FR2583059B1 (fr) | 1985-06-06 | 1987-10-09 | Orstom | Procede de production de spores de champignons filamenteux et dispositif utilise |
JPS61289879A (ja) * | 1985-06-17 | 1986-12-19 | Fuji Electric Co Ltd | 醸造タンクの熟成温度調整装置 |
US4882283A (en) * | 1987-11-17 | 1989-11-21 | Phillips Petroleum Company | Heat exchange apparatus |
AU4925393A (en) * | 1992-09-16 | 1994-04-12 | Rotron Incorporated | Biofilter with modular trays |
FR2701487B1 (fr) | 1993-02-10 | 1995-03-31 | Agronomique Inst Nat Rech | Réacteur pour conduire de façon stérile des procédés de fermentation d'un produit à l'état solide. |
DE4406632C1 (de) | 1994-03-01 | 1995-10-12 | Dietmar Dr Vollbrecht | Feststoff-Bioreaktor |
ID28122A (id) * | 1998-04-30 | 2001-05-03 | Prophyta Biolog Pflanzenschutz | Fermenter keadaan padat dan prosedur untuk fermentasi keadaan dapat |
US6197573B1 (en) * | 1998-11-17 | 2001-03-06 | Biocon India Limited | Solid state fermentation |
US6432698B1 (en) * | 1999-01-06 | 2002-08-13 | Rutgers, The State University | Disposable bioreactor for culturing microorganisms and cells |
DE19943853A1 (de) * | 1999-09-13 | 2001-03-15 | Udo Hoelker | Bioreaktor zur Fermentierung von festen Stoffen |
US6440596B1 (en) * | 1999-10-20 | 2002-08-27 | Technology Management, Inc. | Solid-oxide fuel cell hot assembly |
-
2003
- 2003-07-31 DE DE10335522A patent/DE10335522A1/de not_active Withdrawn
-
2004
- 2004-07-17 RU RU2005133000/13A patent/RU2359026C2/ru active
- 2004-07-17 CN CNB2004800113589A patent/CN100347283C/zh not_active Expired - Fee Related
- 2004-07-17 MX MXPA05011688A patent/MXPA05011688A/es active IP Right Grant
- 2004-07-17 TR TR2005/04027T patent/TR200504027T2/xx unknown
- 2004-07-17 WO PCT/DE2004/001558 patent/WO2005012478A1/de active Application Filing
- 2004-07-17 CA CA2520721A patent/CA2520721C/en not_active Expired - Fee Related
- 2004-07-17 JP JP2006521386A patent/JP4663640B2/ja not_active Expired - Fee Related
- 2004-07-17 AU AU2004261682A patent/AU2004261682B2/en not_active Ceased
- 2004-07-17 BR BRPI0409837-4A patent/BRPI0409837B1/pt not_active IP Right Cessation
- 2004-07-28 US US10/900,581 patent/US7476534B2/en active Active
- 2004-08-02 EP EP04018255A patent/EP1502946B1/de not_active Expired - Lifetime
- 2004-08-02 AT AT04018255T patent/ATE422210T1/de active
- 2004-08-02 PL PL04018255T patent/PL1502946T3/pl unknown
- 2004-08-02 DK DK04018255T patent/DK1502946T3/da active
- 2004-08-02 DE DE502004008945T patent/DE502004008945D1/de not_active Expired - Lifetime
-
2005
- 2005-10-10 CO CO05103244A patent/CO5660305A2/es active IP Right Grant
- 2005-10-31 ZA ZA200508807A patent/ZA200508807B/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP4663640B2 (ja) | 2011-04-06 |
CN1780904A (zh) | 2006-05-31 |
CA2520721C (en) | 2013-01-15 |
CO5660305A2 (es) | 2006-07-31 |
CA2520721A1 (en) | 2005-02-10 |
ZA200508807B (en) | 2006-11-29 |
BRPI0409837A (pt) | 2006-05-09 |
EP1502946A1 (de) | 2005-02-02 |
DE10335522A1 (de) | 2005-02-17 |
JP2007500502A (ja) | 2007-01-18 |
US20050059141A1 (en) | 2005-03-17 |
MXPA05011688A (es) | 2006-01-23 |
PL1502946T3 (pl) | 2009-09-30 |
AU2004261682A1 (en) | 2005-02-10 |
WO2005012478A8 (de) | 2005-11-17 |
RU2359026C2 (ru) | 2009-06-20 |
DE502004008945D1 (de) | 2009-03-19 |
ATE422210T1 (de) | 2009-02-15 |
TR200504027T2 (tr) | 2006-05-22 |
WO2005012478A1 (de) | 2005-02-10 |
US7476534B2 (en) | 2009-01-13 |
AU2004261682B2 (en) | 2008-02-07 |
BRPI0409837B1 (pt) | 2014-01-28 |
CN100347283C (zh) | 2007-11-07 |
RU2005133000A (ru) | 2006-03-20 |
DK1502946T3 (da) | 2009-06-08 |
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