EP1305332A2 - Pharmezeutisch interessante moleküle mit glutaminsäure oder glutamin am n-terminalen ende als salze einer starken säure - Google Patents

Pharmezeutisch interessante moleküle mit glutaminsäure oder glutamin am n-terminalen ende als salze einer starken säure

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Publication number
EP1305332A2
EP1305332A2 EP01919544A EP01919544A EP1305332A2 EP 1305332 A2 EP1305332 A2 EP 1305332A2 EP 01919544 A EP01919544 A EP 01919544A EP 01919544 A EP01919544 A EP 01919544A EP 1305332 A2 EP1305332 A2 EP 1305332A2
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EP
European Patent Office
Prior art keywords
molecule
seq
mhc
glutamine
strong acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP01919544A
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English (en)
French (fr)
Inventor
Christine Klinguer-Hamour
Nathalie Corvaia
Alain Beck
Liliane Goetsch
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Pierre Fabre Medicament SA
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Pierre Fabre Medicament SA
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Application filed by Pierre Fabre Medicament SA filed Critical Pierre Fabre Medicament SA
Publication of EP1305332A2 publication Critical patent/EP1305332A2/de
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Definitions

  • the present invention relates to a molecule of pharmaceutical interest, preferably a ligand of the Complex Histocompatibility major (MHC), comprising a glutamic acid or a glutamine at its N-terminal end, which is in the form of a physiologically acceptable strong acid addition salt, as well as a vaccine comprising such a ligand.
  • MHC Complex Histocompatibility major
  • Vaccination is an effective way to prevent or reduce viral or bacterial infections.
  • Vaccine antigens administered alone in the host are often not immunogenic enough to induce an immune response, and must therefore be combined with an adjuvant or coupled to a carrier protein to induce (or increase) their immunogenicity. Under these conditions, only a humoral type immune response can be induced.
  • CTL cytotoxic T lymphocytes
  • CTL responses and T auxiliary have also been well described for vaccines against parasites such as Plasmodium falciparum, the causative agent of malaria (Le et al, Vaccine, 1998. 16, 305-312).
  • the primordial role of CTL responses has also been well documented in anti-tumor responses, in particular those directed against melanoma cells (reviewed in Rivoltini et al., Crit. Rev. Immunol, 1998, 18, 55-63).
  • the CTL epitope (s) peptide sequences interacting with class I molecules and presented to CD8 + T lymphocytes have been defined for several antigens.
  • MHC ligands The interest of these MHC ligands is confirmed by the increasing number of clinical studies in humans of these compounds as vaccine candidates against various pathologies and in particular as anti-melanoma vaccines (epitopes m27-25 MART 1, g209- 217, g280-288, gplOO, MAGE 3), as an anti-HIN vaccine (Klinguer et al, Vaccine, 2000, 18, 259-267) or also as anti-HBN vaccines of anti-HBN lipopeptide types (Livingston et al, J. Immunol., 1999, 162, 3088-3095).
  • the difficulty of these studies lies in the fact that the peptides used are difficult to keep before their administration to patients, which can lead to a reduction in their vaccine power, and to more rapid degradation in vivo.
  • the peptides having an amino acid of glutamic acid (Glu, E) or glutamine (Gln, Q) type at their N-terminal end cyclize with the free ⁇ -carboxylic acid function to form a pyroglutamate according to the reaction defined above below:
  • This acetylation reaction is however a minor modification of the structure of the peptide conventionally used by a person skilled in the art to improve the stability of a peptide (Brinckerhoff et al, Int. J. Cancer, 1999, 83, 326): it it is the replacement of one of the protons of the N-terminal NH function with an acetyl group CH 3 CO with a slight increase in mass (42 Da over 985 Da), all the rest of the structure remaining unchanged.
  • peptides obtained by chemical synthesis are purified by reverse phase HPLC using eluents containing trifluoroacetic acid (TFA) before being lyophilized.
  • the purified peptides obtained are positively charged and are in the form of trifluoroacetate salt (RNH 3 + , CF 3 CO " ).
  • the amount of trifluoroacetate and residual trifluoroacetic acid is generally proportional to the number of basic amino acids (Lysine, Arginine and Histidine) contained in the sequence as well as in the amino function of the N-terminal amino acid.
  • Peptides in the form of trifluoroacetate are commonly used for pre-clinical in vitro and in vivo experiments in animals.
  • acetic acid is a weak acid, which gives increased instability to the peptide. This forces the investigators to keep the peptide at -80 ° C (liquid nitrogen) in lyophilized form and to resolubilize it extemporaneously just before injection. which involves a very restrictive cold chain.
  • the present invention proposes to solve these problems of structural instability, conservation over time, toxicity and biological activity.
  • molecules of pharmaceutical interest in particular MHC ligands, having a glutamic acid or a glutamine at their N-terminal end can be stabilized in the form of addition salt of a strong acid, and that this makes it possible both to maintain the biological activity and to obtain an easy conservation of the peptide or the like in a stable form, which allows its therapeutic use in humans.
  • molecule of pharmaceutical interest in particular the MHC ligands, the natural or synthetic molecules having an epitope for the generation of antibodies, the molecules derived from receptor ligands, and having an agonist or antagonist activity with respect to these receptors, or having antibiotic, antifungal, or antiviral activity.
  • the molecules of therapeutic interest according to the invention are all characterized in that they have a glutamic acid or a -glutamine at their N-terminal end.
  • the molecules of pharmaceutical interest preferred according to the present invention are the ligands of the MHC.
  • the present invention thus in particular relates to a MHC ligand comprising at its N-terminal end a glutamic acid or a glutamine, characterized in that it is in the form of addition salt of a strong physiologically acceptable acid.
  • the physiologically acceptable strong acid addition salt can in particular be chosen from addition salts with strong mineral or organic acids.
  • methanesulfonate or mesilate
  • hydrochloride hydrobromide
  • sulfate nitrate
  • phosphate phosphate
  • MHC ligands within the meaning of the present invention are in particular MHC class I and IL ligands MHC is an important group of proteins involved in the presentation of antigens to T lymphocytes.
  • MHC class I molecules are membrane proteins found on all nucleated cells and platelets.
  • MHC class II molecules are expressed on B cells, macrophages, monocytes, antigen-presenting cells and certain T cells.
  • B cells are lymphocytes, which in mature form have immunoglobulin on their surface making "antigen receptor” function.
  • T cells are lymphocytes that express their receptor for the antigen (TcR) and differentiate into 2 subpopulations: helper T cells (Th or T helper) and cytotoxic T cells (CTL).
  • Th cells help B cells to divide, differentiate and produce antibodies.
  • the majority of Th are CD4 + (specific surface marker) and recognize the antigen presented on the surface of cells presenting the antigen, in association with MHC class II molecules.
  • Cytotoxic T cells are capable of destroying target cells infected by viruses or allogenic cells.
  • the majority are CD 8+ and recognize the antigen associated with MHC class I molecules on the surface of the target cell.
  • the antigen is recognized by the formation of a complex comprising in particular the MHC molecule having a MHC ligand, and the T cell receptor (TCR).
  • the molecules of pharmaceutical interest in particular the MHC ligands according to the present invention, can be chosen from natural or synthetic molecules, and inter alia, from proteins, peptides, multi-epitopic polypeptide constructs, or analogs of peptides of the pseudopeptide type, retro-inverso, peptoids, peptido-mimetics, lipopeptides. These molecules can also consist in part of a peptide chain, with the replacement of certain amino acids by analogues of amino acids, or having ramifications. These molecules can also present the various modifications which are observed on natural proteins or peptides (for example O- or N-glycosylation).
  • the MHC ligands according to the present invention are chosen from CTL epitopes, that is to say which. allow the generation of cytotoxic T lymphocytes and in particular among those which are in the form of octapeptide, nonapeptide or decapeptide.
  • the MHC ligand can also be chosen from the ligands described in the SYFPEITHI or MHCPEP databases, previously cited, and which comprise at their N-terminal end a glutamic acid or a glutamine.
  • This ligand can in particular be chosen from MHC ligands (ligands of MHC molecules of class I or II) included in the group consisting of peptides corresponding to the sequences SEQ ID No. 1 to SEQ ID No. 694.
  • ELA MART-1 26-35 EAAGIGILTN A2 112 MAGE-1 161-169 EADPTGHSY Al 2
  • the ligands according to the invention can also be chosen from multiepitopic polypeptide constructions having an amino acid of glutamic acid (Glu, E) or glutamine (Gln, Q) type at the N-terminal end such as the following peptide (SEQ ID N ° 695): NEF 117 EWRFDSRLAFHHVAREHPEYFNKNK (PaIm) NH 2
  • anti-HIV lipopeptide in clinical phase I Klinguer, et al, Vaccine, 1999, 18, 259-267.
  • the peptide analogs can be chosen from those described in application FR276307 which comprise at their N-terminal end a glutamic acid or a glutamine.
  • the invention relates to the MHC ligand of sequence ELAGIGILTV, in sulphate form or, even more preferably, in hydrochloride form.
  • the invention also relates to a pharmaceutical composition comprising at least one molecule of pharmaceutical interest according to the invention.
  • compositions can in particular be intended for the treatment of various immunopathologies: immunodeficiency, autoimmune diseases, hypersensitivities, allergies or to avoid rejection of grafts.
  • a composition according to the invention can also be used for an antibiotic, antiviral or antifungal purpose, or can be intended for the treatment of diseases linked to hormonal disorders, or to diseases of the central nervous system.
  • compositions according to the invention can also be used in the veterinary field. Indeed, the same problems of structural instability, conservation over time, toxicity and activity which arise for the preparation of veterinary preparations comprising a peptide or a molecule having a glutamic acid or a glutamine at their N- end terminal, can be resolved using strong acid addition salts to stabilize said peptides or molecules.
  • a preferred composition consists of a vaccine characterized in that it comprises at least one MHC ligand according to the invention, which is in the form of a physiologically acceptable strong acid addition salt , as defined above.
  • This vaccine can also comprise at least one adjuvant, in particular chosen from Aluminum (Alum) or Calcium salts, OmpA enterobacterial proteins, tetanus toxoid (TT), diphtheria toxoid (DT), CRM 197 (cross-reactive material), PLGA, ISCOM, Montanide ISA 720, aliphatic quaternary ammoniums, MPL-A, Quil-A, CpG, Leif, cholera toxin (CT), LT (LT for "Heat labile enterotoxin "heat labile enterotoxin) or detoxified versions of CT or LT.
  • the vaccine further comprises a carrier compound mixed or coupled to said ligand.
  • said carrier compound is chosen from the group of peptides comprising toxoids, in particular diphtheria toxoid (DT) or tetanus toxoid (TT), proteins derived from streptococcus (such as the protein for binding to human serum albumin, called “ BB “described in WO96 / 14415), OmpA membrane proteins (for” Outer Membrane Protein type A ”) and external membrane protein complexes (OMPC), external membrane vesicles (OMV) or heat shock proteins ("Heat Shock Protein” or HSP).
  • said carrier compound is covalently coupled with the ligand.
  • the term “coupling” is intended to denote both a coupling carried out chemically between the two compounds, and a biological coupling, by genetic recombination, as defined below.
  • the covalent coupling of the antigen or hapten can be carried out at the N or C terminus of the carrier compound.
  • the bifunctional reagents allowing this coupling are determined according to the end of the chosen carrier compound and the nature of the antigen or the hapten to be coupled. These coupling techniques are well known to those skilled in the art.
  • the conjugates resulting from a coupling of peptides can also be prepared by genetic recombination.
  • the hybrid (conjugated) peptide can in fact be produced by recombinant DNA techniques by insertion or addition to the DNA sequence coding for the carrier compound, of a sequence coding for the antigen, immunogenic or hapten peptides. These techniques for preparing hybrid peptide by genetic recombination are well known to those skilled in the art (cf. for example Makrides, 1996, Microbiologicals Reviews, 60, 512-538).
  • said carrier compound is a protein derived from streptococcus or an OmpA membrane protein from enterobacteria, in particular from Klebsiella pneumoniae, or one of its fragments.
  • the ligand according to the invention optionally associated with a carrier compound can be incorporated into vectors chosen from liposomes, virosomes, nanospheres, microspheres, microcapsules or biovectors.
  • a person skilled in the art knows how to choose the appropriate vector according to the aim sought (protection of the ligand possibly associated with a carrier compound or an adjuvant from degradation, targeting of cells of interest, search for penetration of the material contained in the vector inside target cells ).
  • One embodiment of the invention relates in particular to an anti-melanoma vaccine characterized in that it comprises at least one ELAGIGILTV peptide (SEQ ID No. 81) in the form of the hydrochloride or sulphate.
  • Another form relates to an anti-melanoma vaccine characterized in that it comprises at least one ELAGIGILTV peptide (SEQ ID No. 81) in the form of the hydrochloride or sulfate and in addition an enterobacterium protein OmpA.
  • Vaccines according to the invention can also be developed for use in the veterinary field, the identical problems of structural instability, storage over time, toxicity and activity being able to be solved in the same way.
  • the subject of the invention is also a method of in vitro diagnosis of pathologies associated with the presence in the body of a patient, of MHC ligands which can interact with MHC molecules, and which may be directly or indirectly involved.
  • MHC ligands which can interact with MHC molecules, and which may be directly or indirectly involved.
  • it comprises the steps of: bringing a biological sample from a patient into contact, in particular blood or any biological sample susceptible to contain lymphocytes, with a MHC ligand according to the invention, under conditions allowing the formation of a binary complex between said MHC ligand and the MHC molecules present in said sample, and the reaction between said binary complex and the T cell receptors that may be present in said biological sample.
  • the diagnostic methods according to the invention are advantageously carried out as follows: incubation of said biological sample with MHC ligands according to the invention, said MHC ligands being fixed on a solid support, in particular inside wells of plates microtitration of the type usually used for the implementation of detection or assay techniques well known under the name of ELISA (Enzyme Linked Immuno Sorbent Assay),
  • Rinsing steps are advantageously carried out between the different steps of this process.
  • Those skilled in the art know how to define the different incubation conditions, as well as the methods for detecting MHC complexes - MHC ligand - T receptor, the use of antibodies being only one method among others.
  • a subject of the invention is also the kits or kits for implementing in vitro diagnostic methods as described above, comprising:
  • reagents for detecting the ternary complex according to the invention which was produced at the end of the immunological reaction, said reagents possibly containing a marker or being capable of being recognized in turn by a labeled reagent, more particularly in the case where the peptide analog is not labeled.
  • the use of the peptides ELAGIGILTV (SEQ ID No. 81), EAAGIGILTV (SEQ ID No. 112), EADPTGHSY (SEQ ID No. 2), or EVDPIGHLY (SEQ ID No. 273) is preferred in a method of diagnosis of melanoma.
  • the peptides QVPLRPMTYK SEQ ID No. 567
  • EIYKRWIIL SEQ ID No. 10
  • EIKDTKEAL SEQ ID No. 692
  • a ligand according to the invention for the preparation of a vaccine intended for the prophylactic or therapeutic treatment of viral, bacterial, parasitic or fungal infections is another object of the invention.
  • the invention also relates to the use of a ligand according to the invention, for the preparation of a vaccine intended for the prophylactic or therapeutic treatment of cancers and preferably for inhibiting the growth of tumors.
  • the present invention also relates to the use of a strong physiologically acceptable acid for stabilizing and maintaining the biological activity of a molecule of pharmaceutical interest comprising a glutamic acid or a glutamine at its " N-terminal " end.
  • the activity which one seeks to maintain is an activity of stimulation and of interaction with the cells of the immune system.
  • the invention also relates to the use of a strong acid to reduce and / or suppress the formation of the pyroglutamic derivative of a molecule of pharmaceutical interest comprising a glutamic acid or a glutamine at its N-terminal end.
  • the present invention relates to a process for the stabilization of a molecule of pharmaceutical interest comprising a glutamic acid or a glutamine at its N-terminal end, characterized in that said molecule is reacted with a strong acid in conditions making it possible to obtain said molecule in the form of a physiologically acceptable strong acid addition salt.
  • the reaction with the strong acid is carried out in particular according to a process as defined below, the strong acid can be chosen from the strong acids defined above, and preferably makes it possible to obtain a hydrochloride.
  • the invention also relates to a process for the preparation of a molecule of pharmaceutical interest comprising a glutamic acid or a glutamine at its N-terminal end in the form of a physiologically acceptable strong acid addition salt according to the invention.
  • This process may in particular comprise a step of purification by RP-HPLC of said molecule from the corresponding trifluoroacetate salt using an eluent based on said strong acid, optionally followed by a step of lyophilization of the solution thus obtained.
  • An alternative method comprises a step of dissolving a trifluoroacetate salt of said molecule in an excess solution of said strong acid, optionally followed by a step of lyophilization of the solution thus obtained.
  • a MHC ligand is preferred, in particular SEQ ID No. 81, 112, 2, 273, 567, 10, 692, 11, 464, 466, 106, 257, or 568. More preferably, it this is SEQ ID No. 81 and the strong acid salt is a hydrochloride.
  • Figure 1 Difference in cell lysis of EL-4 A2 / Kb cells pre-prepursed with the peptide ELA, by lymphocytes obtained after immunization of mice with the peptides ELA (diamonds) or AcELA (squares) in the presence of the adjuvant protein rP40, according to the protocol of Example III ..
  • FIG 3 Chromatogram of the ELA peptide in acetate (3. A) or hydrochloride (3.B) form stored at 37 ° C for two months.
  • Figure 4 Chromatogram of the ELA peptide in hydrochloride form initially (4.A) or after one month of storage at 4 ° C (4.B).
  • ELA peptide the ELA peptide (SEQ ID No. 81) is synthesized in solid phase from the C-terminal amino acid to the N-terminal amino acid (glutamic acid) in FMOC or tBOC chemistry. After cleavage of the resin and of the protective groups of the reactive side chains, the peptide is purified in a conventional manner with eluents based on trifluoroacetic acid / water and trifluoroacetic acid / acetonitrile before being lyophilized. The purity of the peptide is checked by reverse phase liquid chromatography. The amino acid composition is checked after hydrolysis and determination of the derived amino acids obtained. The exact mass is measured by mass spectrometry.
  • Peptide PyrELA PyrELA the peptide is synthesized in the same manner as the ELA peptide to the single "difference of the coupling of the last amino acid the N-terminal: glutamic acid is replaced by a pyroglutamic acid.
  • AcELA peptide the AcELA peptide is synthesized in the same way as the ELA peptide with the only difference of a covering (capping) of glutamic acid using acetic anhydride.
  • This reaction is carried out using the corresponding trifluoroacetate salt, using ion exchange chromatography.
  • Ion exchange resins commercially available in the form of hydrochloride (Dowex resin 1X4, Amberlite IRA 416) are used, which can be used as they are once regenerated.
  • a) Regeneration of the resin the resin to be regenerated is introduced into a wide column equipped with a high porosity frit (1 or 2). The resin is then washed successively with ultra pure water (pH 5-6), IN sodium hydroxide solution (pH 14), ultra pure water (pH 7), IN HCl (pH 1) and a again with ultra pure water (pH 5-6).
  • the resin is stored in an acetonitrile / HCl 10 "4 N (20/80) mixture at room temperature for at least one year.
  • the amount of hydrochloride can be determined by anion exchange chromatography.
  • the amount of trifluoroacetic acid can be determined by gas chromatography.
  • HLA-A * 0201 / Kb (A2 / Kb) transgenic mice of strain C57B1 / 6 x BDA / 2 were used in this study (Vitiello et al., 1991, J. Exp. Med., 173,
  • the MHC class I molecule expressed in these mice is a chimeric molecule formed from the al and a2 domains of the human molecule HLA-A0201
  • A2 / Kb mice received 300 ⁇ g of rP40 mixed with 50 ⁇ g of ELA or 300 ⁇ g of rP40 mixed with 50 ⁇ g of AcELA. a) Generation of effector cytotoxic cells:
  • lymphocytes of the draining ganglia are recovered to be stimulated in vitro with the peptide falling.
  • These lymphocytes (4-5 10 6 ) are cultured in a 24-well plate in DMEM plus 10 mM HEPES, 10% FCS and 50 ⁇ M ⁇ -2-mercaptoefhanol with 2-5 10 5 EL-4 A2 / Kb cells (murine cells transfected with the HLA-A * 0201 / Kb) gene irradiated (10 kRads) pre-pulsed for 1 h at 37 ° C with 1 ⁇ M of the relevant peptide. After two weekly stimulations, the cells are tested for their cytotoxic activity.
  • b) Measurement of cytotoxic activity is a measure the cytotoxic activity:
  • the A2 / Kb EL-4 cells are incubated for 1 h with 51 Cr in the presence or not of ELA, washed and then co-incubated with the effector cells at different ratio in 96-well plate in a volume of 200 ⁇ l for 4 to 6 h at 37 ° C. The cells are then centrifuged and the release of 51 Cr is measured in 100 ⁇ l of supernatant. The percentage of specific lysis is calculated as follows:
  • % specific lysis % lysis with cells pulsed by the peptide -% lysis with cells not pulsed by the peptide.
  • Comparative example IN CTL activity of the peptides ELA, PyrELA and AcELA
  • mice received:
  • lymphocytes (4-5 10 6 ) are cultured in a 24-well plate in DMEM plus lOmM HEPES, 10% FCS and 50 ⁇ M ⁇ -2-mercaptoethanol with 2-5 10 5 EL- 4 A2 / Kb cells (murine cells transfected with the HLA-A * 0201 / Kb gene) irradiated (10 kRads) 1 h at 37 ° C with 1 ⁇ M of the relevant peptide. After two weekly stimulations, the cells are tested for their cytotoxic activity.
  • cytotoxic activity a measurement of cytotoxic activity:
  • the A2 / Kb EL-4 cells are incubated for 1 h with 51 Cr in the presence or not of ELA, washed and then co-incubated with the effector cells at different ratio in 96-well plate in a volume of 200 ⁇ l for 4 to 6 h at 37 ° C. The cells are then centrifuged and the release of 51 Cr is measured in 100 ⁇ l of supernatant. The percentage of specific lysis is calculated as follows:
  • Example V Studies of accelerated stability of the acetate and hydrochloride forms of the peptide ELA. Peptides are analyzed by reverse phase HPLC using an eluent
  • FIG. 3 shows the chromatograms of the ELA peptide in the form of acetate (3.A) or hydrochloride (3.B) stored at 37 ° C for 2 months.
  • Example NI Stability of the ELA peptide in the form of the hydrochloride stored at 4 ° C.
  • the ELA peptide in the hydrochloride form is extremely stable at 4 ° C. It can therefore be easily handled and stored at a temperature of 4 or -20 ⁇ ° C. This is not the case for an equivalent peptide (MART 3), prepared in the form of acetate which must be stored at -80 ° C (M. Marchand et al, Int.
  • the strong acid saline form therefore allows a much easier conservation at 4 ° C (refrigerator) or at -20 ° C (freezer) with total physicochemical stability, as shown in the examples above.

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EP01919544A 2000-03-23 2001-03-22 Pharmezeutisch interessante moleküle mit glutaminsäure oder glutamin am n-terminalen ende als salze einer starken säure Withdrawn EP1305332A2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0003711A FR2806727A1 (fr) 2000-03-23 2000-03-23 Molecule d'interet pharmaceutique comprotant en son extremite n-terminale un acide glutamique ou une glutamine sous forme de sel d'addition d'acide fort physiologiquement acceptable
FR0003711 2000-03-23
PCT/FR2001/000872 WO2001070772A2 (fr) 2000-03-23 2001-03-22 Molecule d'interet pharmaceutique comportant en son extremite n-terminale un acide glutamique ou une glutamine sous forme de sel d'addition d'acide

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CN (1) CN1449407A (de)
AU (1) AU2001246623A1 (de)
BR (1) BR0109502A (de)
CA (1) CA2403803A1 (de)
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BR0109502A (pt) 2004-01-13
CA2403803A1 (fr) 2001-09-27
AU2001246623A1 (en) 2001-10-03
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