EP1263971A1 - Procede permettant d'influencer la production de pollen par modification du metabolisme du saccharose - Google Patents
Procede permettant d'influencer la production de pollen par modification du metabolisme du saccharoseInfo
- Publication number
- EP1263971A1 EP1263971A1 EP01907515A EP01907515A EP1263971A1 EP 1263971 A1 EP1263971 A1 EP 1263971A1 EP 01907515 A EP01907515 A EP 01907515A EP 01907515 A EP01907515 A EP 01907515A EP 1263971 A1 EP1263971 A1 EP 1263971A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- plant
- nucleic acid
- protein
- acid molecule
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims abstract description 40
- 229930006000 Sucrose Natural products 0.000 title claims abstract description 37
- 239000005720 sucrose Substances 0.000 title claims abstract description 37
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 title claims abstract description 36
- 230000008119 pollen development Effects 0.000 title claims abstract description 12
- 230000004060 metabolic process Effects 0.000 title claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 156
- 108010047540 sucrose isomerase Proteins 0.000 claims abstract description 115
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 85
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 78
- 230000014509 gene expression Effects 0.000 claims abstract description 73
- 230000009261 transgenic effect Effects 0.000 claims abstract description 69
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 56
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 53
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 53
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 53
- 230000001105 regulatory effect Effects 0.000 claims abstract description 23
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000003306 harvesting Methods 0.000 claims abstract description 6
- 210000004027 cell Anatomy 0.000 claims description 91
- 108010025855 palatinase Proteins 0.000 claims description 53
- 241000556426 Erwinia rhapontici Species 0.000 claims description 37
- 238000004519 manufacturing process Methods 0.000 claims description 30
- 239000013598 vector Substances 0.000 claims description 30
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 21
- 235000011073 invertase Nutrition 0.000 claims description 21
- 238000012546 transfer Methods 0.000 claims description 19
- 230000008488 polyadenylation Effects 0.000 claims description 15
- 238000013518 transcription Methods 0.000 claims description 15
- 230000035897 transcription Effects 0.000 claims description 15
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 14
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 13
- 230000000692 anti-sense effect Effects 0.000 claims description 13
- 230000035558 fertility Effects 0.000 claims description 13
- 150000001720 carbohydrates Chemical class 0.000 claims description 12
- 235000014633 carbohydrates Nutrition 0.000 claims description 12
- 239000001573 invertase Substances 0.000 claims description 12
- 102000005455 Monosaccharide Transport Proteins Human genes 0.000 claims description 11
- 108010006769 Monosaccharide Transport Proteins Proteins 0.000 claims description 11
- 102000005548 Hexokinase Human genes 0.000 claims description 9
- 108700040460 Hexokinases Proteins 0.000 claims description 9
- 108020004999 messenger RNA Proteins 0.000 claims description 8
- 108091006112 ATPases Proteins 0.000 claims description 7
- 102000057290 Adenosine Triphosphatases Human genes 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 7
- 238000011069 regeneration method Methods 0.000 claims description 7
- 230000013595 glycosylation Effects 0.000 claims description 6
- 238000006206 glycosylation reaction Methods 0.000 claims description 6
- 150000002402 hexoses Chemical class 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 6
- 210000001938 protoplast Anatomy 0.000 claims description 6
- 230000008929 regeneration Effects 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 230000023852 carbohydrate metabolic process Effects 0.000 claims description 4
- 235000021256 carbohydrate metabolism Nutrition 0.000 claims description 4
- 108090000994 Catalytic RNA Proteins 0.000 claims description 3
- 102000053642 Catalytic RNA Human genes 0.000 claims description 3
- 108091092562 ribozyme Proteins 0.000 claims description 3
- 229940118465 Isomerase inhibitor Drugs 0.000 claims description 2
- 230000004071 biological effect Effects 0.000 claims description 2
- 238000005520 cutting process Methods 0.000 claims description 2
- 239000003394 isomerase inhibitor Substances 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 description 178
- 239000012634 fragment Substances 0.000 description 64
- 239000013615 primer Substances 0.000 description 56
- 108020004414 DNA Proteins 0.000 description 54
- 235000018102 proteins Nutrition 0.000 description 53
- 239000013612 plasmid Substances 0.000 description 47
- 239000002773 nucleotide Substances 0.000 description 44
- 125000003729 nucleotide group Chemical group 0.000 description 44
- 244000061176 Nicotiana tabacum Species 0.000 description 31
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 31
- 230000000694 effects Effects 0.000 description 30
- PVXPPJIGRGXGCY-DJHAAKORSA-N 6-O-alpha-D-glucopyranosyl-alpha-D-fructofuranose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@](O)(CO)O1 PVXPPJIGRGXGCY-DJHAAKORSA-N 0.000 description 22
- 238000003752 polymerase chain reaction Methods 0.000 description 20
- 241000588724 Escherichia coli Species 0.000 description 19
- 244000061456 Solanum tuberosum Species 0.000 description 19
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 19
- 235000002595 Solanum tuberosum Nutrition 0.000 description 17
- 230000009466 transformation Effects 0.000 description 16
- 210000002421 cell wall Anatomy 0.000 description 15
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 15
- 108091026890 Coding region Proteins 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 14
- 108090000790 Enzymes Proteins 0.000 description 14
- 230000003321 amplification Effects 0.000 description 14
- 238000003199 nucleic acid amplification method Methods 0.000 description 14
- 108010076504 Protein Sorting Signals Proteins 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000003112 inhibitor Substances 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 238000013459 approach Methods 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- 241000701489 Cauliflower mosaic virus Species 0.000 description 11
- 238000009396 hybridization Methods 0.000 description 11
- 230000005764 inhibitory process Effects 0.000 description 11
- 101150062179 II gene Proteins 0.000 description 10
- 239000002253 acid Substances 0.000 description 10
- 150000007513 acids Chemical class 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 10
- 150000002016 disaccharides Chemical class 0.000 description 10
- 230000035772 mutation Effects 0.000 description 10
- 108091008146 restriction endonucleases Proteins 0.000 description 10
- 210000001519 tissue Anatomy 0.000 description 10
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 9
- 238000012300 Sequence Analysis Methods 0.000 description 9
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 9
- 238000010367 cloning Methods 0.000 description 9
- 244000038559 crop plants Species 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 241000589158 Agrobacterium Species 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 239000002299 complementary DNA Substances 0.000 description 8
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 8
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 8
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 8
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 8
- 238000004925 denaturation Methods 0.000 description 8
- 230000036425 denaturation Effects 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 239000011535 reaction buffer Substances 0.000 description 8
- 239000011541 reaction mixture Substances 0.000 description 8
- 206010021929 Infertility male Diseases 0.000 description 7
- 208000007466 Male Infertility Diseases 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 7
- 108020001507 fusion proteins Proteins 0.000 description 7
- 102000037865 fusion proteins Human genes 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 150000002772 monosaccharides Chemical group 0.000 description 7
- 238000006116 polymerization reaction Methods 0.000 description 7
- SVBWNHOBPFJIRU-UHFFFAOYSA-N 1-O-alpha-D-Glucopyranosyl-D-fructose Natural products OC1C(O)C(O)C(CO)OC1OCC1(O)C(O)C(O)C(O)CO1 SVBWNHOBPFJIRU-UHFFFAOYSA-N 0.000 description 6
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 6
- 229930091371 Fructose Natural products 0.000 description 6
- 239000005715 Fructose Substances 0.000 description 6
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 240000002791 Brassica napus Species 0.000 description 5
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 5
- 241000588698 Erwinia Species 0.000 description 5
- 101710196632 LexA repressor Proteins 0.000 description 5
- 108700026244 Open Reading Frames Proteins 0.000 description 5
- 238000012408 PCR amplification Methods 0.000 description 5
- 108010083644 Ribonucleases Proteins 0.000 description 5
- 102000006382 Ribonucleases Human genes 0.000 description 5
- 241001622809 Serratia plymuthica Species 0.000 description 5
- 238000010170 biological method Methods 0.000 description 5
- 238000003776 cleavage reaction Methods 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 238000010353 genetic engineering Methods 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 230000007017 scission Effects 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- NMXLJRHBJVMYPD-IPFGBZKGSA-N trehalulose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(O)CO[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 NMXLJRHBJVMYPD-IPFGBZKGSA-N 0.000 description 5
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 4
- 235000011293 Brassica napus Nutrition 0.000 description 4
- 108020004511 Recombinant DNA Proteins 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 4
- 230000002759 chromosomal effect Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000006317 isomerization reaction Methods 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 3
- 241000219194 Arabidopsis Species 0.000 description 3
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 3
- 241000588914 Enterobacter Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 102000004157 Hydrolases Human genes 0.000 description 3
- 108090000604 Hydrolases Proteins 0.000 description 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 108700001094 Plant Genes Proteins 0.000 description 3
- 108091034057 RNA (poly(A)) Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 3
- 239000004473 Threonine Substances 0.000 description 3
- 108700009124 Transcription Initiation Site Proteins 0.000 description 3
- 241000083686 [Pseudomonas] mesoacidophila Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 235000004279 alanine Nutrition 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 229930003935 flavonoid Natural products 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 235000017173 flavonoids Nutrition 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 239000006870 ms-medium Substances 0.000 description 3
- 230000002018 overexpression Effects 0.000 description 3
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 2
- 244000060924 Brassica campestris Species 0.000 description 2
- 235000005637 Brassica campestris Nutrition 0.000 description 2
- 108700010070 Codon Usage Proteins 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 235000002634 Solanum Nutrition 0.000 description 2
- 241000207763 Solanum Species 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 240000006677 Vicia faba Species 0.000 description 2
- 235000010749 Vicia faba Nutrition 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000003139 biocide Substances 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 229940088530 claforan Drugs 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000005849 recognition of pollen Effects 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000009210 therapy by ultrasound Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 1
- NMKJJVNCRCSYDT-UHFFFAOYSA-N 1-benzylpurin-2-amine Chemical compound NC1=NC2=NC=NC2=CN1CC1=CC=CC=C1 NMKJJVNCRCSYDT-UHFFFAOYSA-N 0.000 description 1
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 101150114578 APG gene Proteins 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000207875 Antirrhinum Species 0.000 description 1
- 240000001436 Antirrhinum majus Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- RWCLSUOSKWTXLA-FXQIFTODSA-N Arg-Asp-Ala Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(O)=O RWCLSUOSKWTXLA-FXQIFTODSA-N 0.000 description 1
- BKFXFUPYETWGGA-XVSYOHENSA-N Asn-Phe-Thr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(O)=O BKFXFUPYETWGGA-XVSYOHENSA-N 0.000 description 1
- QCLHLXDWRKOHRR-GUBZILKMSA-N Asp-Glu-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)N QCLHLXDWRKOHRR-GUBZILKMSA-N 0.000 description 1
- 108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 235000021533 Beta vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 101150111062 C gene Proteins 0.000 description 1
- 101100275473 Caenorhabditis elegans ctc-3 gene Proteins 0.000 description 1
- 229920000018 Callose Polymers 0.000 description 1
- 240000004160 Capsicum annuum Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 101100406840 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) palF gene Proteins 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- QVXWAFZDWRLXTI-NWLDYVSISA-N Glu-Thr-Trp Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CCC(=O)O)N)O QVXWAFZDWRLXTI-NWLDYVSISA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- UWSMZKRTOZEGDD-CUJWVEQBSA-N His-Thr-Ser Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(O)=O UWSMZKRTOZEGDD-CUJWVEQBSA-N 0.000 description 1
- 102000005385 Intramolecular Transferases Human genes 0.000 description 1
- 108010031311 Intramolecular Transferases Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- 108010054278 Lac Repressors Proteins 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 241000192130 Leuconostoc mesenteroides Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- 101710132674 Monosaccharide transporter Proteins 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- MGDFPGCFVJFITQ-CIUDSAMLSA-N Pro-Glu-Asp Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O MGDFPGCFVJFITQ-CIUDSAMLSA-N 0.000 description 1
- 241000586779 Protaminobacter Species 0.000 description 1
- 238000012181 QIAquick gel extraction kit Methods 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 235000003846 Ricinus Nutrition 0.000 description 1
- 241000322381 Ricinus <louse> Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000012377 Salvia columbariae var. columbariae Nutrition 0.000 description 1
- 240000005481 Salvia hispanica Species 0.000 description 1
- 235000001498 Salvia hispanica Nutrition 0.000 description 1
- JPIDMRXXNMIVKY-VZFHVOOUSA-N Ser-Ala-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O JPIDMRXXNMIVKY-VZFHVOOUSA-N 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 101100020617 Solanum lycopersicum LAT52 gene Proteins 0.000 description 1
- 101100083698 Solanum lycopersicum LAT56 gene Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 229940100389 Sulfonylurea Drugs 0.000 description 1
- 101150085122 TA-29 gene Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 108050004197 Trp repressor Proteins 0.000 description 1
- BZMIYHIJVVJPCK-QSFUFRPTSA-N Val-Ile-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](C(C)C)N BZMIYHIJVVJPCK-QSFUFRPTSA-N 0.000 description 1
- DFQZDQPLWBSFEJ-LSJOCFKGSA-N Val-Val-Asn Chemical compound CC(C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)O)N DFQZDQPLWBSFEJ-LSJOCFKGSA-N 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000008848 allosteric regulation Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003392 amylase inhibitor Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940019748 antifibrinolytic proteinase inhibitors Drugs 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 101150082190 chiB gene Proteins 0.000 description 1
- 235000014167 chia Nutrition 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 108010060641 flavanone synthetase Proteins 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 108010085325 histidylproline Proteins 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 101150106875 malE gene Proteins 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 101150041450 palH gene Proteins 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 101150114322 ro gene Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
Definitions
- tissue- or cell-specific promoters are also preferably used for antisense or sense constructs in order to restrict changes in the carbohydrate metabolism with the aim of undersupplying the pollen to the relevant tissues.
- the present invention thus relates to a recombinant nucleic acid molecule comprising a) regulatory sequences of a promoter active in anthers, in tapetum and / or in pollen; b) operatively linked to a DNA sequence encoding a protein with the enzymatic activity of a sucrose isomerase; and c) operatively linked regulatory sequences which can serve as transcription, termination and or polyadenylation signals in plant cells.
- the DNA sequence which encodes a protein with the enzymatic activity of a sucrose isomerase is selected from the group consisting of a) DNA sequences which comprise a nucleotide sequence which has the sequence shown in SEQ ID NO. 6 encode the given amino acid sequence or fragments thereof, b) DNA sequences which contain the sequence shown in SEQ ID No.
- the invention further relates to vectors and microorganisms which contain nucleic acid molecules according to the invention and whose use enables the production of male-sterile plants.
- the vectors are in particular plasmids, cosmids, viruses, bacteriophages and other vectors commonly used in genetic engineering.
- the microorganisms are primarily bacteria, viruses, fungi, yeasts and algae.
- the invention further relates to a method for producing male-sterile plants, comprising the following steps: a) Production of a recombinant nucleic acid molecule which comprises the following sequences: regulatory sequences of one in anthers. promoters active in the tapetum and / or pollen; operatively linked to it a DNA sequence encoding a protein with the enzymatic activity of a sucrose isomerase; and operatively linked to regulatory sequences that can serve as transcription, termination and / or polyadenylation signals in plant cells; b) transfer of the nucleic acid molecule from a) to plant cells and c) regeneration of transgenic plants.
- a recombinant nucleic acid molecule which comprises the following sequences: regulatory sequences of one in anthers. promoters active in the tapetum and / or pollen; operatively linked to it a DNA sequence encoding a protein with the enzymatic activity of a sucrose isomerase; and operatively linked to regulatory sequences
- palatinase DNA sequences which code for an enzyme with high affinity for palatinose are preferably used. Accordingly, those trehalulase DNA sequences are preferably used which code for an enzyme with high affinity for trehalulose.
- LexA repressor binds hybrids to the LexA operator region and thereby prevents the transcription of the sucrose isomerase gene.
- LexA operator DNA molecules can be obtained, for example, by the synthesis of DNA fragments, which contain LexA operator sequences well known to those skilled in the literature, for example by Garriga et al. (1992) Mol. Gen. Genet. 236: 125.
- DNA sequences which code for the LexA repressor can be obtained, for example, by synthesis of such DNA molecules or by DNA cloning techniques as are known to the person skilled in the art and are described, for example, by Garriga et al., Vide supra.
- sequences coding for the LexA repressor can be taken, for example, from plasmid pRB500 (ATTC 67758).
- Fragment A contains the 35S promoter of the Cauliflower Mosaic Virus (CaMV). It contains a fragment which comprises nucleotides 6909 to 7437 of the CaMV (Franck (1980) Cell 21: 285.
- Fragment B contains nucleotides 923-1059 of a proteinase inhibitor II gene from the potato (Keil et al., Supra ), which is fused via a linker with the sequence ACC GAA TTG GG to the sucrose isomerase gene from Erwinia rhapontici, which comprises nucleotides 109-1803, thereby making a signal peptide necessary for the uptake of proteins into the endoplasmic reticulum (ER) vegetable protein N-terminally fused to the sucrose isomerase sequence.
- CaMV Cauliflower Mosaic Virus
- Termination signal includes the 3 'end of the polyadenylation site of the octopine synthase gene.
- the plasmid pTA29-cwIso contains three fragments A, B and C, which were cloned into the sites for restriction enzymes of the polylinker of pUC18 (see Figure 3).
- Fragment A contains the TA29 promoter from Nicotiana tabacum.
- the fragment contains the nucleotides -1477 to +57 relative to the transcription initiation site of the TA29 gene (Seurinck et al. (1990) Nucl. Acids. Res. 18: 3403). It was generated by PCR from genomic DNA from Nicotiana tabacum Var. Samsun NN amplified.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
L'invention concerne un procédé permettant d'influencer la production de pollen par modification du métabolisme du saccharose dans des cellules de plantes et des plantes transgéniques. L'invention concerne notamment un procédé de production de plantes stériles de type mâle, dans lequel du saccharose est extrait du pollen. Plus particulièrement, l'invention concerne l'expression d'une protéine ayant l'activité enzymatique d'une isomérase de saccharose dans des cellules de plantes transgéniques. En outre, l'invention concerne des molécules d'acide nucléique renfermant une séquence ADN codant pour une protéine à activité enzymatique d'une saccharose-isomérase, et dans laquelle la séquence ADN est liée coopérante avec des séquences régulatrices d'un promoteur actif dans des plantes, de telle sorte que la séquence ADN soit exprimée en anthères ou pollen. De plus, l'invention concerne des plantes et des cellules de plantes transgéniques qui renferment la molécule d'acide nucléique selon l'invention et qui, sur la base de l'expression de la séquence ADN, codent pour une protéine à activité enzymatique d'une saccharose-isomérase, et qui, par ailleurs, sont stériles de type mâle, ainsi que des produits récoltés et des matériaux de propagation des plantes transgéniques.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10006413 | 2000-02-14 | ||
| DE10006413 | 2000-02-14 | ||
| DE10045113 | 2000-09-13 | ||
| DE10045113A DE10045113A1 (de) | 2000-02-14 | 2000-09-13 | Verfahren zur Beeinflussung der Pollenentwicklung durch Veränderung des Saccharosestoffwechsels |
| PCT/EP2001/001412 WO2001059135A1 (fr) | 2000-02-14 | 2001-02-09 | Procede permettant d'influencer la production de pollen par modification du metabolisme du saccharose |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP1263971A1 true EP1263971A1 (fr) | 2002-12-11 |
Family
ID=26004322
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP01907515A Withdrawn EP1263971A1 (fr) | 2000-02-14 | 2001-02-09 | Procede permettant d'influencer la production de pollen par modification du metabolisme du saccharose |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20030159181A1 (fr) |
| EP (1) | EP1263971A1 (fr) |
| AR (1) | AR027421A1 (fr) |
| AU (1) | AU2001235460A1 (fr) |
| WO (1) | WO2001059135A1 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPQ976800A0 (en) * | 2000-08-29 | 2000-09-21 | University Of Queensland, The | Novel polypeptides and polynucleotides and uses therefor |
| DE10047286B4 (de) * | 2000-09-20 | 2005-06-09 | Südzucker AG Mannheim/Ochsenfurt | Isomalt produzierende transgene Pflanze |
| US7572950B2 (en) | 2002-07-04 | 2009-08-11 | Sungene Gmbh & Co. Kgaa | Methods for obtaining pathogen resistance in plants |
| AU2003246353A1 (en) * | 2002-07-04 | 2004-01-23 | Sungene Gmbh And Co. Kgaa | Methods for obtaining pathogen resistance in plants |
| AU2003902253A0 (en) | 2003-05-12 | 2003-05-29 | The University Of Queensland | Method for increasing product yield |
| US20050120418A1 (en) * | 2003-11-06 | 2005-06-02 | Anawah Inc. | Tomatoes having altered acid invertase activity due to non-transgenic alterations in acid invertase genes |
| WO2009147179A2 (fr) | 2008-06-03 | 2009-12-10 | Nordsaat Saatzuchtgesellschaft Mbh | Procédé de fabrication de plantes monocotylédones stériles mâles |
| EP2423316B1 (fr) | 2010-08-25 | 2016-11-16 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) | Procédé pour déterminer la fréquence de la recombinaison meiotique dans les plantes |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9028060D0 (en) * | 1990-12-24 | 1991-02-13 | Nickerson Int Seed | Recombinant dna |
| DE4447471C2 (de) * | 1994-01-19 | 1995-12-21 | Suedzucker Ag | Protein mit Palatinase-Aktivität und dafür codierende Nukleinsäure |
-
2001
- 2001-02-09 AU AU2001235460A patent/AU2001235460A1/en not_active Abandoned
- 2001-02-09 EP EP01907515A patent/EP1263971A1/fr not_active Withdrawn
- 2001-02-09 WO PCT/EP2001/001412 patent/WO2001059135A1/fr not_active Application Discontinuation
- 2001-02-14 AR ARP010100655A patent/AR027421A1/es unknown
-
2002
- 2002-08-14 US US10/223,277 patent/US20030159181A1/en not_active Abandoned
Non-Patent Citations (1)
| Title |
|---|
| See references of WO0159135A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001059135A1 (fr) | 2001-08-16 |
| US20030159181A1 (en) | 2003-08-21 |
| AR027421A1 (es) | 2003-03-26 |
| AU2001235460A1 (en) | 2001-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0973921B1 (fr) | Sequences d'adn codant pour une 2-desoxyglucose-6-phosphate (2-dog-6-p) phosphatase et servant de marqueur de selection chez les vegetaux | |
| DE60017781T2 (de) | Modulation der antwort einer pflanze auf abscisin säure | |
| DE69836267T2 (de) | Nukleinsäuren aus der artischocke (cynara scolymus), welche für ein enzym mit fruktosylpolymerase-aktivität kodieren | |
| EP1038014B1 (fr) | SEQUENCES DE (GntI) VEGETALES ET UTILISATION DE CELLES-CI POUR L'OBTENTION DE PLANTES NE PRESENTANT PAS UNE ACTIVITE DE N-ACETYLGLUCOSYLAMINYLTRANSFERASE I GnTI OU BIEN SEULEMENT UNE TELLE ACTIVITE REDUITE | |
| CN101182523B (zh) | 植物花药特异性启动子及其应用 | |
| DE4447387A1 (de) | Debranching-Enzyme aus Pflanzen und DNA-Sequenzen kodierend diese Enzyme | |
| DE4441408A1 (de) | DNA-Sequenzen aus Solanum tuberosum kodierend Enzyme, die an der Stärkesynthese beteiligt sind, Plasmide, Bakterien, Pflanzenzellen und transgene Pflanzen enhaltend diese Sequenzen | |
| DE19509695A1 (de) | Verfahren zur Herstellung einer modifizieren Stärke in Pflanzen, sowie die aus den Pflanzen isolierbare modifizierte Stärke | |
| DE4035756A1 (de) | Neue plasmide zur herstellung von im habitus und ertrag veraenderten transgenen pflanzen | |
| DE69434173T2 (de) | Herstellung von trehalose in pflanzen | |
| WO1996021030A1 (fr) | Accumulation accrue du trehalose dans les plantes | |
| EP1263971A1 (fr) | Procede permettant d'influencer la production de pollen par modification du metabolisme du saccharose | |
| AU697450B2 (en) | Processes for inhibiting and for inducing flower formation in plants | |
| EP1272645B1 (fr) | Production de sucres non cariogenes dans des plantes transgeniques | |
| EP0909314A4 (fr) | Plantes detectant les sucres et leurs utilisations | |
| US6025544A (en) | Processes for modifying plant flowering behavior | |
| EP0967278A2 (fr) | Gène pour la régulation de l'induction de la floraison et son utilisation | |
| DE10047286B4 (de) | Isomalt produzierende transgene Pflanze | |
| DE10045113A1 (de) | Verfahren zur Beeinflussung der Pollenentwicklung durch Veränderung des Saccharosestoffwechsels | |
| DE10045182A1 (de) | Verwendung von Palatinase- und Trehalulase-Sequenzen als nutritive Marker in transformierten Zellen | |
| EP1255841A2 (fr) | Utilisation de sequences de palatinase et de trehalulase en tant que marqueurs nutritifs dans des cellules transformees | |
| WO2000060101A1 (fr) | Marqueurs de selection metaboliques destines aux plantes | |
| EP0979874A2 (fr) | Séquence de protéine et d'ADN encodant la pinosylvine-3-O-méthyltransférase (PMT) | |
| DE10050233A1 (de) | Verfahren zur genetischen Modifizierung einer Pflanze | |
| JPWO2005001088A1 (ja) | 植物培養細胞で糖欠乏により働くプロモーターの有用タンパク質の分泌生産への適用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 20020905 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR |
|
| AX | Request for extension of the european patent |
Free format text: AL PAYMENT 20020905;LT PAYMENT 20020905;LV PAYMENT 20020905;MK PAYMENT 20020905;RO PAYMENT 20020905;SI PAYMENT 20020905 |
|
| GRAP | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOSNIGR1 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
| 18D | Application deemed to be withdrawn |
Effective date: 20060328 |