EP1092041A4 - Exonuklease-vermittelter zusammenbau von nukleinsäuren in der direkten evolution - Google Patents

Exonuklease-vermittelter zusammenbau von nukleinsäuren in der direkten evolution

Info

Publication number
EP1092041A4
EP1092041A4 EP00919763A EP00919763A EP1092041A4 EP 1092041 A4 EP1092041 A4 EP 1092041A4 EP 00919763 A EP00919763 A EP 00919763A EP 00919763 A EP00919763 A EP 00919763A EP 1092041 A4 EP1092041 A4 EP 1092041A4
Authority
EP
European Patent Office
Prior art keywords
exonuclease
nucleic acid
directed evolution
mediated nucleic
acid reassembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00919763A
Other languages
English (en)
French (fr)
Other versions
EP1092041A1 (de
Inventor
Jay M Short
Tsotne David Djavakhishvili
Gerhard Johann Frey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of EP1092041A1 publication Critical patent/EP1092041A1/de
Publication of EP1092041A4 publication Critical patent/EP1092041A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
EP00919763A 1999-03-26 2000-03-27 Exonuklease-vermittelter zusammenbau von nukleinsäuren in der direkten evolution Withdrawn EP1092041A4 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US276860 1988-11-28
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US332835 1999-06-14
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
PCT/US2000/008245 WO2000058517A1 (en) 1999-03-26 2000-03-27 Exonuclease-mediated nucleic acid reassembly in directed evolution

Publications (2)

Publication Number Publication Date
EP1092041A1 EP1092041A1 (de) 2001-04-18
EP1092041A4 true EP1092041A4 (de) 2001-09-19

Family

ID=26958167

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00919763A Withdrawn EP1092041A4 (de) 1999-03-26 2000-03-27 Exonuklease-vermittelter zusammenbau von nukleinsäuren in der direkten evolution

Country Status (6)

Country Link
EP (1) EP1092041A4 (de)
JP (1) JP2004500019A (de)
AU (1) AU4039400A (de)
CA (1) CA2329122A1 (de)
MX (1) MXPA00011569A (de)
WO (1) WO2000058517A1 (de)

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US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
EP1119616A2 (de) 1998-10-07 2001-08-01 Maxygen, Inc. Dna shuffling zur herstellung von nukleinsäuren zur mykotoxinentgiftung
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
IL138206A (en) * 1999-02-04 2011-06-30 Verenium Corp Methods for non-stochastic generation of progeny polypeptides and hybrid polynucleotides
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4589602B2 (ja) 2000-12-01 2010-12-01 ヴェレニウム コーポレイション デハロゲナーゼ活性を有する酵素およびその利用方法
DE60239386D1 (de) 2001-01-25 2011-04-21 Evolva Ltd Zellbibliothek
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
EP1840211A1 (de) 2001-10-31 2007-10-03 Danisco A/S Pyranoson Dehydratase aus phanerochaete Chrysosporium
EP1619961B1 (de) 2003-01-17 2010-11-10 Danisco A/S Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
BRPI0417533B1 (pt) 2003-12-24 2016-03-01 Danisco enzima variante glicolipídio aciltransferase, uso e método para sua produção, método para o preparo de um produto alimentício e produto assado de farinha, processo de refinamento enzimático
EP2267108A1 (de) 2004-07-16 2010-12-29 Danisco A/S Enzymatisches Ölentschleimungsverfahren
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
JP5789073B2 (ja) * 2005-01-10 2015-10-07 メッドイミューン・リミテッド 変異誘発方法
EP2109670A1 (de) 2007-01-25 2009-10-21 Danisco A/S Herstellung einer lipid-acyltransferase aus transformierten bacillus licheniformis-zellen
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
EP2139515B2 (de) 2007-03-30 2023-12-20 The Research Foundation of the State University of New York Für impfstoffe geeignete abgeschwächte viren
AU2008339660B2 (en) 2007-12-21 2013-09-26 Dupont Nutrition Biosciences Aps Process for edible oil refining using a lipid acyltransferase
EP2406399B1 (de) 2009-03-09 2018-02-14 Bioatla, LLC Mirac-proteine
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
US20120058222A1 (en) 2009-05-19 2012-03-08 Danisco A/S Use
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
CA2788148C (en) 2010-03-12 2018-03-13 Dupont Nutrition Biosciences Aps An enzymatic process for refining a plant oil
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
BR112012032176A2 (pt) 2010-06-17 2015-09-08 Dupont Nutrition Biosci Aps processo de tratamento de sementes que contém óleo, método de obtenção de óleo de sementes vegetais, processo de produção de um óleo vegetal refinado e óleo vegetal bruto ou refinado que pode ser obtido por meio de um método ou processo
GB201011513D0 (en) 2010-07-08 2010-08-25 Danisco Method
CN103459606B (zh) 2011-02-23 2017-06-16 杜邦营养生物科学有限公司 加工方法
EP2678427A2 (de) 2011-02-23 2014-01-01 DuPont Nutrition Biosciences ApS Verfahren zur herstellung rekombinanter enzyme mit fähigkeit zur hydrolyse von chlorophyll oder chlorophyllderivaten
AR085251A1 (es) 2011-02-23 2013-09-18 Danisco Proceso para tratar aceite vegetal
KR20140024365A (ko) 2011-04-08 2014-02-28 다니스코 유에스 인크. 조성물
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
EP2909230B1 (de) 2012-10-19 2019-05-22 Danisco US Inc. Verfahren zur stabilisierung von biometrischen membranen
CA2934039A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Use of hydrophobins to increase gas transferin aerobic fermentation processes
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
EP3209774B1 (de) 2014-10-24 2020-02-26 DuPont Nutrition Biosciences ApS Verwendung von prolintoleranten tripeptidylpeptidasen in futterzusatzzusammensetzungen
US20170306360A1 (en) 2014-10-24 2017-10-26 Danisco Us Inc. Method for producing alcohol by use of a tripeptidyl peptidase
WO2017147060A1 (en) 2016-02-25 2017-08-31 Dupont Nutrition Biosciences Aps Method for producing a protein hydrolysate employing an aspergillus fumigatus tripeptidyl peptidase
EP3491128B1 (de) * 2016-07-29 2021-09-08 New England Biolabs, Inc. Verfahren und zusammensetzungen zur verhinderung der concatemerisierung während der vorlagenumschaltung

Citations (9)

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Publication number Priority date Publication date Assignee Title
EP0316018A2 (de) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
EP0438292A2 (de) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Verfahren zur Vorbereitung von Primern für die Polymerase-Kettenreaktion und DNS-Verstärkung durch Verwendung dieser Primer
WO1991012341A1 (en) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Improved method for ordered deletions from a fixed point in a cloned insert
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
WO1997020950A1 (en) * 1995-12-08 1997-06-12 Stratagene Improved circular site-directed mutagenesis
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
WO1998038297A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Single step assembly of multiple dna fragments
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Family Cites Families (2)

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US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316018A2 (de) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
EP0438292A2 (de) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Verfahren zur Vorbereitung von Primern für die Polymerase-Kettenreaktion und DNS-Verstärkung durch Verwendung dieser Primer
WO1991012341A1 (en) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Improved method for ordered deletions from a fixed point in a cloned insert
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
WO1997020950A1 (en) * 1995-12-08 1997-06-12 Stratagene Improved circular site-directed mutagenesis
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
WO1998038297A1 (en) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Single step assembly of multiple dna fragments
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function

Non-Patent Citations (5)

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Title
G.P. SMITH: "The progeny of sexual PCR", NATURE, vol. 370, 4 August 1994 (1994-08-04), pages 324 - 325 *
PALERMO D.P.; HESS G.F.: "Use of lambda exonuclease for efficient oligonucleotide-mediated site-directed deletion and point mutation of double stranded DNA", DNA, vol. 6, no. 3, pages 273 - 279 *
See also references of WO0058517A1 *
STEMMER W: "DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 91, no. 22, 25 October 1994 (1994-10-25), pages 10747 - 10751, XP002170160 *
WAYNE M. COCO ET AL.: "DNA shuffling method for generating highly recombined genes and evolved enzymes", NATURE BIOTECHNOLOGY, vol. 19, no. 4, pages 354 - 359 *

Also Published As

Publication number Publication date
EP1092041A1 (de) 2001-04-18
JP2004500019A (ja) 2004-01-08
MXPA00011569A (es) 2004-12-03
CA2329122A1 (en) 2000-10-05
WO2000058517A1 (en) 2000-10-05
AU4039400A (en) 2000-10-16

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