EP1092041A4 - Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease - Google Patents

Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Info

Publication number
EP1092041A4
EP1092041A4 EP00919763A EP00919763A EP1092041A4 EP 1092041 A4 EP1092041 A4 EP 1092041A4 EP 00919763 A EP00919763 A EP 00919763A EP 00919763 A EP00919763 A EP 00919763A EP 1092041 A4 EP1092041 A4 EP 1092041A4
Authority
EP
European Patent Office
Prior art keywords
exonuclease
nucleic acid
directed evolution
mediated nucleic
acid reassembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00919763A
Other languages
German (de)
English (en)
Other versions
EP1092041A1 (fr
Inventor
Jay M Short
Tsotne David Djavakhishvili
Gerhard Johann Frey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Enzymes LLC
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of EP1092041A1 publication Critical patent/EP1092041A1/fr
Publication of EP1092041A4 publication Critical patent/EP1092041A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
EP00919763A 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease Withdrawn EP1092041A4 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US276860 1999-03-26
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US332835 1999-06-14
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
PCT/US2000/008245 WO2000058517A1 (fr) 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Publications (2)

Publication Number Publication Date
EP1092041A1 EP1092041A1 (fr) 2001-04-18
EP1092041A4 true EP1092041A4 (fr) 2001-09-19

Family

ID=26958167

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00919763A Withdrawn EP1092041A4 (fr) 1999-03-26 2000-03-27 Reassemblage d'acide nucleique en evolution dirigee induit par l'exonuclease

Country Status (6)

Country Link
EP (1) EP1092041A4 (fr)
JP (1) JP2004500019A (fr)
AU (1) AU4039400A (fr)
CA (1) CA2329122A1 (fr)
MX (1) MXPA00011569A (fr)
WO (1) WO2000058517A1 (fr)

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US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
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US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
US7078504B2 (en) * 2000-12-01 2006-07-18 Diversa Corporation Enzymes having dehalogenase activity and methods of use thereof
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
ATE501251T1 (de) 2001-01-25 2011-03-15 Evolva Ltd Zellbibliothek
CN100591212C (zh) 2001-05-18 2010-02-24 丹尼斯科有限公司 改善生面团和面包质量的方法
EP1840211A1 (fr) 2001-10-31 2007-10-03 Danisco A/S Pyranosone dehydratase de phanerochaete chrysosporium
EP1619961B1 (fr) 2003-01-17 2010-11-10 Danisco A/S Procede pour la production in situ d'un emulsifiant dans un aliment
BRPI0417533B1 (pt) 2003-12-24 2016-03-01 Danisco enzima variante glicolipídio aciltransferase, uso e método para sua produção, método para o preparo de um produto alimentício e produto assado de farinha, processo de refinamento enzimático
EP1776455B1 (fr) 2004-07-16 2015-03-18 DuPont Nutrition Biosciences ApS Enzymes lipotiques, utilisations de ceux-ci dans l'industrie alimentaire
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GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
PT1844144E (pt) * 2005-01-10 2010-06-11 Medimmune Ltd Método de mutagénese
PL2405007T3 (pl) 2007-01-25 2014-04-30 Dupont Nutrition Biosci Aps Wytwarzanie acylotransferazy lipidowej z przekształconych komórek gospodarza Bacillus licheniformis
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
KR101621100B1 (ko) 2007-03-30 2016-05-13 더 리서치 파운데이션 오브 스테이트 유니버시티 오브 뉴욕 백신에 유용한 약독화 바이러스
CN101978037B (zh) 2007-12-21 2015-06-17 杜邦营养生物科学有限公司 使用脂质酰基转移酶精炼食用油的方法
KR102265757B1 (ko) 2009-03-09 2021-06-17 바이오아트라, 인코퍼레이티드 미락 단백질
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
WO2010134035A1 (fr) 2009-05-19 2010-11-25 Danisco A/S Utilisation
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
MX2012010399A (es) 2010-03-12 2012-10-05 Dupont Nutrition Biosci Aps Proceso.
WO2011114251A1 (fr) 2010-03-18 2011-09-22 Danisco A/S Produits alimentaires
US20130085287A1 (en) 2010-06-17 2013-04-04 Dupont Nutrition Biosciences Aps Process
GB201011513D0 (en) 2010-07-08 2010-08-25 Danisco Method
CA3004088A1 (fr) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Traitement enzymatique d'une huile vegetale
CN103797114A (zh) 2011-02-23 2014-05-14 杜邦营养生物科学有限公司 生产能够水解叶绿素或叶绿素衍生物的重组酶的方法
AR085251A1 (es) 2011-02-23 2013-09-18 Danisco Proceso para tratar aceite vegetal
CA2830579A1 (fr) 2011-04-08 2012-10-11 Danisco Us Inc. Compositions
WO2013104659A2 (fr) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Procédé
WO2013104660A1 (fr) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Procédé de traitement d'une huile végétale comprenant l'hydrolyse de chlorophylle ou d'un dérivé de chlorophylle et une neutralisation caustique partielle
WO2013160374A1 (fr) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Procédé de raffinage d'huile végétale brute comprenant une hydrolyse enzymatique et un recyclage de gommes
WO2013160372A1 (fr) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Procédé de traitement d'huile végétale comprenant l'ajout de doses successives de chlorophylle ou d'une enzyme de dégradation de dérivé de chlorophylle
EP2909230B1 (fr) 2012-10-19 2019-05-22 Danisco US Inc. Stabilisation de membranes biométriques
EP3083936B1 (fr) 2013-12-19 2018-07-04 Danisco US Inc. Utilisation d'hydrophobines pour augmenter le transfert de gaz dans des processus de fermentation aérobie
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
DK3209774T3 (da) 2014-10-24 2020-05-25 Dupont Nutrition Biosci Aps Anvendelse af prolintolerante tripeptidylpeptidaser i foderadditivsammensætninger
WO2016065238A1 (fr) 2014-10-24 2016-04-28 Danisco Us Inc. Procédé de production d'alcool au moyen d'une tripeptidyl peptidase
WO2017147060A1 (fr) 2016-02-25 2017-08-31 Dupont Nutrition Biosciences Aps Procédé de production d'un hydrolysat protéique utilisant la tripeptidyl-peptidase aspergillus
EP3491128B1 (fr) * 2016-07-29 2021-09-08 New England Biolabs, Inc. Procédés et compositions destinés à prévenir la concatémérisation pendant la commutation de matrice

Citations (9)

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Publication number Priority date Publication date Assignee Title
EP0316018A2 (fr) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modification de séquences d'ADN
EP0438292A2 (fr) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Procédé pour la préparation d'amorces pour la réaction en chaîne de polymérase à l'aide de ces amorces
WO1991012341A1 (fr) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Procede ameliore pour deletions ordonnees a partir d'un point fixe dans un insert clone
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO1997020918A1 (fr) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Procede de detection d'une activite enzymatique
WO1997020950A1 (fr) * 1995-12-08 1997-06-12 Stratagene Mutagenese amelioree, dirigee sur un site d'un adn circulaire
WO1998001581A1 (fr) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Methode de remaniement d'adn avec des polynucleotides obtenus par blocage ou interruption d'un processus de synthese ou d'amplification
WO1998038297A1 (fr) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Reunion, en une seule operation, de plusieurs fragments d'adn
WO1998058080A1 (fr) * 1997-06-16 1998-12-23 Bioinvent International Ab Procede permettant l'evolution moleculaire in vitro d'une fonction proteique

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US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316018A2 (fr) * 1985-03-29 1989-05-17 Cetus Oncology Corporation Modification de séquences d'ADN
EP0438292A2 (fr) * 1990-01-17 1991-07-24 NIHON CHEMICAL RESEARCH KABUSHIKI KAISHA also known as JCR PHARMACEUTICALS CO., LTD Procédé pour la préparation d'amorces pour la réaction en chaîne de polymérase à l'aide de ces amorces
WO1991012341A1 (fr) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Procede ameliore pour deletions ordonnees a partir d'un point fixe dans un insert clone
WO1997020078A1 (fr) * 1995-11-30 1997-06-05 Maxygen, Inc. Procede d'elaboration de polynucleotides presentant des caracteristiques desirees par selection iterative et recombinaison
WO1997020918A1 (fr) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Procede de detection d'une activite enzymatique
WO1997020950A1 (fr) * 1995-12-08 1997-06-12 Stratagene Mutagenese amelioree, dirigee sur un site d'un adn circulaire
WO1998001581A1 (fr) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Methode de remaniement d'adn avec des polynucleotides obtenus par blocage ou interruption d'un processus de synthese ou d'amplification
WO1998038297A1 (fr) * 1997-02-27 1998-09-03 Gesher - Israel Advanced Biotecs (1996) Ltd. Reunion, en une seule operation, de plusieurs fragments d'adn
WO1998058080A1 (fr) * 1997-06-16 1998-12-23 Bioinvent International Ab Procede permettant l'evolution moleculaire in vitro d'une fonction proteique

Non-Patent Citations (5)

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Title
G.P. SMITH: "The progeny of sexual PCR", NATURE, vol. 370, 4 August 1994 (1994-08-04), pages 324 - 325 *
PALERMO D.P.; HESS G.F.: "Use of lambda exonuclease for efficient oligonucleotide-mediated site-directed deletion and point mutation of double stranded DNA", DNA, vol. 6, no. 3, pages 273 - 279 *
See also references of WO0058517A1 *
STEMMER W: "DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 91, no. 22, 25 October 1994 (1994-10-25), pages 10747 - 10751, XP002170160 *
WAYNE M. COCO ET AL.: "DNA shuffling method for generating highly recombined genes and evolved enzymes", NATURE BIOTECHNOLOGY, vol. 19, no. 4, pages 354 - 359 *

Also Published As

Publication number Publication date
EP1092041A1 (fr) 2001-04-18
WO2000058517A1 (fr) 2000-10-05
CA2329122A1 (fr) 2000-10-05
JP2004500019A (ja) 2004-01-08
MXPA00011569A (es) 2004-12-03
AU4039400A (en) 2000-10-16

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