JP2004500019A - 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合 - Google Patents

定方向進化におけるエキソヌクレアーゼ仲介核酸再集合 Download PDF

Info

Publication number
JP2004500019A
JP2004500019A JP2000608795A JP2000608795A JP2004500019A JP 2004500019 A JP2004500019 A JP 2004500019A JP 2000608795 A JP2000608795 A JP 2000608795A JP 2000608795 A JP2000608795 A JP 2000608795A JP 2004500019 A JP2004500019 A JP 2004500019A
Authority
JP
Japan
Prior art keywords
polynucleotide
nucleic acid
exonuclease
polynucleotides
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000608795A
Other languages
English (en)
Japanese (ja)
Inventor
ショート、ジェイ、エム．
ヤワキシュヴィリ，ソーネ，デイヴィッド
フレイ，ゲルハルド，ジョアン
Original Assignee
ディベルサ　コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/276,860 external-priority patent/US6352842B1/en
Priority claimed from US09/332,835 external-priority patent/US6537776B1/en
Application filed by ディベルサ　コーポレーション filed Critical ディベルサ　コーポレーション
Publication of JP2004500019A publication Critical patent/JP2004500019A/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2000608795A 1999-03-26 2000-03-27 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合 Pending JP2004500019A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/276,860 US6352842B1 (en) 1995-12-07 1999-03-26 Exonucease-mediated gene assembly in directed evolution
US09/332,835 US6537776B1 (en) 1999-06-14 1999-06-14 Synthetic ligation reassembly in directed evolution
PCT/US2000/008245 WO2000058517A1 (en) 1999-03-26 2000-03-27 Exonuclease-mediated nucleic acid reassembly in directed evolution

Publications (1)

Publication Number Publication Date
JP2004500019A true JP2004500019A (ja) 2004-01-08

Family

ID=26958167

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000608795A Pending JP2004500019A (ja) 1999-03-26 2000-03-27 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合

Country Status (6)

Country Link
EP (1) EP1092041A4 (de)
JP (1) JP2004500019A (de)
AU (1) AU4039400A (de)
CA (1) CA2329122A1 (de)
MX (1) MXPA00011569A (de)
WO (1) WO2000058517A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012519499A (ja) * 2009-03-09 2012-08-30 バイオアトラ、エルエルシー Ｍｉｒａｃタンパク質

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
CA2345203A1 (en) 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
MXPA00009723A (es) * 1999-02-04 2003-09-22 Diversa Corp Generacion no estocastica de vacunas y enzimas geneticas.
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
DE19953854C2 (de) * 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
EP1507870A4 (de) * 2000-12-01 2005-11-09 Diversa Corp Enzyme mit dehalogenase-aktivität und verfahren zu deren verwendung
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
BR0209154A (pt) 2001-05-18 2004-07-20 Danisco Processo de preparação de uma massa com uma enzima
EP1840211A1 (de) 2001-10-31 2007-10-03 Danisco A/S Pyranoson Dehydratase aus phanerochaete Chrysosporium
ATE506440T1 (de) 2003-01-17 2011-05-15 Danisco Verfahren zur herstellung eines hydroxysäureesters
WO2005066347A1 (en) 2003-12-24 2005-07-21 Danisco A/S Proteins
DK1776455T3 (da) 2004-07-16 2015-06-22 Dupont Nutrition Biosci Aps Lipolytisk enzym, anvendelser deraf i fødevareindustrien
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
GB0500417D0 (en) 2005-01-10 2005-02-16 Cambridge Antibody Tech Method of mutagenesis
DE602006013596D1 (de) * 2005-01-10 2010-05-27 Medimmune Ltd Cambridge Mutageneseverfahren
BRPI0720801A2 (pt) 2007-01-25 2014-09-16 Dupont Nutrition Biosci Aps Produção de um lipídio aciltranfersase a partir de células transformadas de bacillus licheniformis.
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
US9476032B2 (en) 2007-03-30 2016-10-25 The Research Foundation For The State University Of New York Attenuated viruses useful for vaccines
EA020035B1 (ru) 2007-12-21 2014-08-29 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
EP2432876A1 (de) 2009-05-19 2012-03-28 Danisco A/S Verwendung von inhibitoren der zdhhc2-aktivität zur modulation der adipogenese
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
WO2011110967A1 (en) 2010-03-12 2011-09-15 Danisco A/S Process
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
AR081950A1 (es) 2010-06-17 2012-10-31 Danisco Proceso de tratamiento de semillas que contienen aceite
GB201011513D0 (en) 2010-07-08 2010-08-25 Danisco Method
CA2825628A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Method
WO2012114232A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
ES2649912T3 (es) 2011-02-23 2018-01-16 Dupont Nutrition Biosciences Aps Tratamiento enzimático de la clorofila en los aceites vegetales
RU2013149861A (ru) 2011-04-08 2015-05-20 ДАНИСКО ЮЭс, ИНК. Композиции
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
EP2909230B1 (de) 2012-10-19 2019-05-22 Danisco US Inc. Verfahren zur stabilisierung von biometrischen membranen
DK3083936T3 (en) 2013-12-19 2018-10-22 Danisco Us Inc USE OF HYDROPHOBINES TO INCREASE GAS TRANSFER IN AEROBE FERMENTATION PROCESSES
WO2015150372A1 (en) 2014-04-01 2015-10-08 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
BR112017007949A2 (pt) 2014-10-24 2018-01-23 Danisco Us Inc método para produção de álcool pelo uso de uma tripeptidil-peptidase
EP3209773B1 (de) 2014-10-24 2020-03-18 DuPont Nutrition Biosciences ApS Prolintolerante tripeptidylpeptidasen und verwendungen davon
MX2018009216A (es) 2016-02-25 2018-11-09 Dupont Nutrition Biosci Aps Metodo para producir un hidrolizado de proteina empleando una tripeptidil peptidasa de aspergillus fumigatus.
EP3491128B1 (de) * 2016-07-29 2021-09-08 New England Biolabs, Inc. Verfahren und zusammensetzungen zur verhinderung der concatemerisierung während der vorlagenumschaltung

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0316018A3 (de) * 1985-03-29 1989-07-26 Cetus Oncology Corporation Modifikation von DNS-Sequenzen
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
JP3096722B2 (ja) * 1990-01-17 2000-10-10 日本ケミカルリサーチ株式会社 ポリメラーゼ・チェーン・リアクション用プライマーの作製法およびそれを用いるｄｎａの増幅法
WO1991012341A1 (en) * 1990-02-12 1991-08-22 Fred Hutchinson Cancer Research Center Improved method for ordered deletions from a fixed point in a cloned insert
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO1997020918A1 (en) * 1995-12-07 1997-06-12 Recombinant Biocatalysis, Inc. Method of screening for enzyme activity
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5789166A (en) * 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
IL120338A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Single step DNA fragments assembly
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012519499A (ja) * 2009-03-09 2012-08-30 バイオアトラ、エルエルシー Ｍｉｒａｃタンパク質
US9464284B2 (en) 2009-03-09 2016-10-11 Bioatla, Llc Mirac proteins
US9637734B2 (en) 2009-03-09 2017-05-02 Bioatla, Llc Mirac proteins
US9637735B2 (en) 2009-03-09 2017-05-02 Bioatla, Llc Mirac proteins
US9982252B2 (en) 2009-03-09 2018-05-29 Bioatla, Llc Mirac proteins
US9994841B2 (en) 2009-03-09 2018-06-12 Bioatla, Llc Mirac proteins
US11718844B2 (en) 2009-03-09 2023-08-08 Bioatla, Inc. Mirac proteins

Also Published As

Publication number Publication date
CA2329122A1 (en) 2000-10-05
MXPA00011569A (es) 2004-12-03
EP1092041A4 (de) 2001-09-19
AU4039400A (en) 2000-10-16
EP1092041A1 (de) 2001-04-18
WO2000058517A1 (en) 2000-10-05

Similar Documents

Publication Publication Date Title
CA2374667C (en) Synthetic ligation reassembly in directed evolution
US6358709B1 (en) End selection in directed evolution
US6773900B2 (en) End selection in directed evolution
US6361974B1 (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
JP2004500019A (ja) 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合
US6709841B2 (en) Exonuclease-mediated gene assembly in directed evolution
US6238884B1 (en) End selection in directed evolution
WO2000053744A9 (en) End selection in directed evolution
US6939689B2 (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
AU2009212959B2 (en) Synthetic ligation reassembly in directed evolution
AU2005203018A1 (en) Exonuclease-mediated nucleic acid reassembly in directed evolution
Class et al. Patent application title: SYNTHETIC LIGATION REASSEMBLY IN DIRECTED EVOLUTION Inventors: Jay M. Short (Del Mar, CA, US)
AU2005203719A1 (en) End selection in directed evolution

Legal Events

Date Code Title Description
A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20050405

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20050629

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20050719

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20051213