EP1085083A4 - Kulturmedium zur kultivierung tierischer zellen und verfahren zur herstellung von proteinen durch dessen verwendung - Google Patents

Kulturmedium zur kultivierung tierischer zellen und verfahren zur herstellung von proteinen durch dessen verwendung

Info

Publication number
EP1085083A4
EP1085083A4 EP99922611A EP99922611A EP1085083A4 EP 1085083 A4 EP1085083 A4 EP 1085083A4 EP 99922611 A EP99922611 A EP 99922611A EP 99922611 A EP99922611 A EP 99922611A EP 1085083 A4 EP1085083 A4 EP 1085083A4
Authority
EP
European Patent Office
Prior art keywords
culture
culture medium
same
animal cells
producing proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP99922611A
Other languages
English (en)
French (fr)
Other versions
EP1085083B1 (de
EP1085083A1 (de
Inventor
Kazushi Shibuya
Masaru Atsumi
Shigeyuki Tsunakawa
Kaneo Nogaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chugai Pharmaceutical Co Ltd
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of EP1085083A1 publication Critical patent/EP1085083A1/de
Publication of EP1085083A4 publication Critical patent/EP1085083A4/de
Application granted granted Critical
Publication of EP1085083B1 publication Critical patent/EP1085083B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/40Nucleotides, nucleosides or bases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/70Undefined extracts
    • C12N2500/80Undefined extracts from animals

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
EP99922611A 1998-06-01 1999-06-01 Kulturmedium zur kultivierung tierischer zellen und verfahren zur herstellung von proteinen durch dessen verwendung Expired - Lifetime EP1085083B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP15095798 1998-06-01
JP15095798 1998-06-01
PCT/JP1999/002904 WO1999063058A1 (en) 1998-06-01 1999-06-01 Media for culturing animal cells and process for producing protein by using the same

Publications (3)

Publication Number Publication Date
EP1085083A1 EP1085083A1 (de) 2001-03-21
EP1085083A4 true EP1085083A4 (de) 2002-11-27
EP1085083B1 EP1085083B1 (de) 2007-05-02

Family

ID=15508136

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99922611A Expired - Lifetime EP1085083B1 (de) 1998-06-01 1999-06-01 Kulturmedium zur kultivierung tierischer zellen und verfahren zur herstellung von proteinen durch dessen verwendung

Country Status (15)

Country Link
US (2) US6537782B1 (de)
EP (1) EP1085083B1 (de)
JP (1) JP3510858B2 (de)
KR (1) KR100430447B1 (de)
CN (1) CN1187441C (de)
AT (1) ATE361360T1 (de)
AU (1) AU744715B2 (de)
CA (1) CA2333966C (de)
DE (1) DE69935978T2 (de)
DK (1) DK1085083T3 (de)
ES (1) ES2285840T3 (de)
IL (1) IL139974A (de)
NO (2) NO326180B1 (de)
RU (2) RU2214455C2 (de)
WO (1) WO1999063058A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU736282B2 (en) 1997-03-21 2001-07-26 Chugai Seiyaku Kabushiki Kaisha A preventive or therapeutic agent for sensitized T cell- mediated diseases comprising IL-6 antagonist as an active ingredient
DE60009956T2 (de) * 1999-12-28 2005-04-28 Iso Tis N.V. Wachstumsfaktoren und l-glutamin enthaltendes zellkulturmedium
UA80091C2 (en) 2001-04-02 2007-08-27 Chugai Pharmaceutical Co Ltd Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist
DE10124820A1 (de) * 2001-05-21 2002-12-05 Heinrich Wieland Zusammensetzung und Verwendung von Substanzen zur Stabilisierung von schwefelhaltigen Aminosäuren im Blut
WO2003068260A1 (fr) 2002-02-14 2003-08-21 Chugai Seiyaku Kabushiki Kaisha Produits pharmaceutiques en solution contenant des anticorps
GB2401040A (en) 2003-04-28 2004-11-03 Chugai Pharmaceutical Co Ltd Method for treating interleukin-6 related diseases
JPWO2005090405A1 (ja) 2004-03-24 2008-04-17 中外製薬株式会社 インターロイキン−6受容体に対するヒト型化抗体のサブタイプ
TWI374935B (en) * 2004-08-27 2012-10-21 Pfizer Ireland Pharmaceuticals Production of α-abeta
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
KR100679112B1 (ko) * 2004-11-17 2007-02-07 삼성정밀화학 주식회사 동물세포 배양방법
DK1835022T3 (en) * 2005-01-05 2015-02-23 Chugai Pharmaceutical Co Ltd CELL CULTIVATION PROCEDURE AND UTILIZATION OF THIS
US7972810B2 (en) 2005-12-08 2011-07-05 Amgen Inc. Production of glycoproteins using manganese
CA2633306A1 (en) 2006-01-04 2007-07-12 Baxter International Inc. Oligopeptide-free cell culture media
PE20091174A1 (es) * 2007-12-27 2009-08-03 Chugai Pharmaceutical Co Ltd Formulacion liquida con contenido de alta concentracion de anticuerpo
EP4406615A3 (de) 2009-10-26 2024-12-11 F. Hoffmann-La Roche AG Verfahren zur herstellung eines glykosylierten immunglobulins
MD191Z5 (ro) * 2010-01-21 2010-11-30 Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова Mediu nutritiv pentru cultivarea celulelor de origine animală
EP2638067A2 (de) 2010-11-08 2013-09-18 Genentech, Inc. Subkutan verabreichter anti-il-6-rezeptorantikörper
KR102248581B1 (ko) 2013-07-04 2021-05-06 에프. 호프만-라 로슈 아게 혈청 샘플 중 항-약물 항체를 검출하기 위한 간섭-억제된 면역분석
WO2015066377A1 (en) 2013-10-30 2015-05-07 The Curators Of The University Of Missouri Method for scalable skeletal muscle lineage specification and cultivation
AU2016268709B2 (en) 2015-05-27 2022-01-13 Astellas Pharma Inc. Cell culturing method using nucleic acid-containing medium
WO2017147169A1 (en) 2016-02-22 2017-08-31 Ohio State Innovation Foundation Chemoprevention using controlled-release formulations of anti-interleukin 6 agents, synthetic vitamin a analogues or metabolites, and estradiol metabolites
EP3596175A4 (de) 2017-03-17 2021-01-13 The Ohio State Innovation Foundation Nanopartikel zur abgabe von chemopräventiven mitteln
EP3612623B1 (de) 2017-05-06 2024-07-03 Upside Foods, Inc. Zusammensetzungen und verfahren zur erhöhung der kulturdichte einer zellbiomasse in einer kultivationsinfrastruktur
EP3638777A4 (de) 2017-07-13 2021-05-12 Memphis Meats, Inc. Zusammensetzungen und verfahren zur erhöhung der effizienz von zellkulturen zur nahrungsmittelherstellung
WO2020201362A2 (en) 2019-04-02 2020-10-08 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods of predicting and preventing cancer in patients having premalignant lesions
KR20230124607A (ko) 2020-12-22 2023-08-25 가부시키가이샤 마루하치 무라마쓰 펩타이드, 세포 증식 촉진제, 단백질 산생 촉진제,배지, 그 펩타이드를 이용한 세포 증식 방법, 및, 그 펩타이드를 이용한 단백질 산생 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3856627A (en) * 1972-12-11 1974-12-24 Morinaga Milk Industry Co Ltd Culture medium for bacteria
JPH0239882A (ja) * 1988-07-29 1990-02-08 Japan Synthetic Rubber Co Ltd 動物細胞培養用培地
WO1995023212A1 (en) * 1994-02-23 1995-08-31 Sea Run Holdings, Inc. Method for culturing mammalian cells and insect cells in a medium containing fish serum
CN1157694A (zh) * 1996-10-15 1997-08-27 玉环县海天营养源有限公司 一种液化鱼蛋白粉的制作方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS509877B2 (de) * 1972-09-13 1975-04-16
US3962425A (en) * 1972-09-21 1976-06-08 Weyerhaeuser Company Ruminant repellent from enzymatically putrefied lipoidal material
JPH01148181A (ja) * 1987-12-07 1989-06-09 Jgc Corp グルタチオンの菌体内蓄積法
JPH0638704A (ja) * 1991-06-19 1994-02-15 Morinaga Milk Ind Co Ltd 発酵調味料及びその製造法
US5426045A (en) 1993-12-16 1995-06-20 Sea Run Holdings, Inc. Method for culturing mammalian cells in a medium containing fish serum
RU2103360C1 (ru) * 1996-07-25 1998-01-27 Инна Георгиевна Ермишина Питательная среда для культивирования клеток эукариотов и способ получения основы питательной среды - протеолитического гидролизата

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3856627A (en) * 1972-12-11 1974-12-24 Morinaga Milk Industry Co Ltd Culture medium for bacteria
JPH0239882A (ja) * 1988-07-29 1990-02-08 Japan Synthetic Rubber Co Ltd 動物細胞培養用培地
WO1995023212A1 (en) * 1994-02-23 1995-08-31 Sea Run Holdings, Inc. Method for culturing mammalian cells and insect cells in a medium containing fish serum
CN1157694A (zh) * 1996-10-15 1997-08-27 玉环县海天营养源有限公司 一种液化鱼蛋白粉的制作方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 200140, Derwent World Patents Index; AN 2001-375305 [40], XP002214165 *
PATENT ABSTRACTS OF JAPAN vol. 014, no. 197 (C - 0712) 23 April 1990 (1990-04-23) *

Also Published As

Publication number Publication date
RU2214455C2 (ru) 2003-10-20
KR20010071359A (ko) 2001-07-28
HK1037678A1 (en) 2002-02-15
DK1085083T3 (da) 2007-06-18
DE69935978T2 (de) 2008-01-17
EP1085083B1 (de) 2007-05-02
KR100430447B1 (ko) 2004-05-10
AU3958499A (en) 1999-12-20
NO20006096L (no) 2001-02-01
IL139974A0 (en) 2002-02-10
IL139974A (en) 2005-09-25
NO326180B1 (no) 2008-10-13
CN1309698A (zh) 2001-08-22
AU744715C (en) 1999-12-20
US6537782B1 (en) 2003-03-25
DE69935978D1 (de) 2007-06-14
AU744715B2 (en) 2002-02-28
CA2333966C (en) 2006-07-25
RU2333242C2 (ru) 2008-09-10
CA2333966A1 (en) 1999-12-09
JP3510858B2 (ja) 2004-03-29
NO2009012I1 (no) 2009-06-15
NO20006096D0 (no) 2000-11-30
ES2285840T3 (es) 2007-11-16
CN1187441C (zh) 2005-02-02
WO1999063058A1 (en) 1999-12-09
EP1085083A1 (de) 2001-03-21
US6962812B2 (en) 2005-11-08
US20030096372A1 (en) 2003-05-22
ATE361360T1 (de) 2007-05-15

Similar Documents

Publication Publication Date Title
EP1085083A4 (de) Kulturmedium zur kultivierung tierischer zellen und verfahren zur herstellung von proteinen durch dessen verwendung
DE69637656D1 (de) Methoden zur Züchtung von Tierzellen
ES8700320A1 (es) Metodo de obtencion del exprimido de una materia seleccionada que contiene renina.
MX9703063A (es) Compuestos de ester y acido boronico, su sintesis y usos.
CY1117558T1 (el) Μεθοδος για καλλιεργεια κυτταρων για παραγωγη ουσιων
DK1451290T3 (da) Indretning og fremgangsmåde til udførelse af fermentering af høj celledensitet
NO970514D0 (no) Bakteriell produksjon av hydrofobiske polypeptider
FR2540381B1 (fr) Procede pour stimuler la croissance des cellules; composition cosmetique, pharmaceutique et composition complementaire pour milieu de culture cellulaire appliquant ce procede
DE69030765D1 (de) Medien zur zellzucht und verfahren dazu
RU2000133243A (ru) Питательная среда для культуры животной клетки (варианты) и способ получения белка с использованием среды
ATE353953T1 (de) Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion
DK1495112T3 (da) Cellekulturmedium
DE69426723D1 (de) Nährboden zur Züchtung tierischer Zellen oder Zellen die Antikörper produzieren
EP0649900A3 (de) Verfahren zur Herstellung von Proteinen.
ES534117A0 (es) Procedimiento para la obtencion de sarcosin-oxidasa
EA200100023A1 (ru) Промышленный способ производства гетерологичных белков в пригодных для этого способа штаммах е.coli
EP1227155A4 (de) Verfahren zur herstellung von ubiquinon-10
ES2160829T3 (es) Procedimiento para la obtencion de riboflavina por medio de microorganismos con actividad de isocitratoliasa modificada.
EP1176194A4 (de) Verfahren der zellkultivierung
FR2801900B1 (fr) Methode de production de cellules animales adherentes
ATE288476T1 (de) Herstellung von proteinen
FR2671098B1 (fr) Milieu haute densite pour culture de cellules animales.
ES2074509T3 (es) Metodo para cultivar protoplastos de tomate.
ATE56988T1 (de) Verfahren zur submersen zuechtung von pseudomonas aeruginosa-bakterienstaemmen.

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20001222

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

A4 Supplementary search report drawn up and despatched

Effective date: 20021010

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20070502

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REF Corresponds to:

Ref document number: 69935978

Country of ref document: DE

Date of ref document: 20070614

Kind code of ref document: P

REG Reference to a national code

Ref country code: DK

Ref legal event code: T3

REG Reference to a national code

Ref country code: SE

Ref legal event code: TRGR

ET Fr: translation filed
REG Reference to a national code

Ref country code: CH

Ref legal event code: NV

Representative=s name: E. BLUM & CO. AG PATENT- UND MARKENANWAELTE VSP

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2285840

Country of ref document: ES

Kind code of ref document: T3

REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1034748

Country of ref document: HK

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20071002

Ref country code: MC

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20070630

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20080205

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20070803

REG Reference to a national code

Ref country code: IE

Ref legal event code: SPCF

Free format text: SPC017/2009: 20090527

Spc suppl protection certif: SPC017/2009

Filing date: 20090527

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20070502

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20070601

REG Reference to a national code

Ref country code: IE

Ref legal event code: DD9A

REG Reference to a national code

Ref country code: IE

Ref legal event code: SPCG

Free format text: PRODUCT NAME: TOCILIZUMAB-HUMANISED IGG1 MONOCLONAL ANTIBODY AGAINST THE HUMAN INTERLEUKIN-6 (IL-6) RECEPTOR.; REGISTRATION NO/DATE: EU/1/08/492/001-006 20090116

Spc suppl protection certif: 2009/017

Extension date: 20240115

Effective date: 20140217

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 18

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 19

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20180614

Year of fee payment: 20

Ref country code: IE

Payment date: 20180612

Year of fee payment: 20

Ref country code: DE

Payment date: 20180522

Year of fee payment: 20

Ref country code: DK

Payment date: 20180517

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: AT

Payment date: 20180509

Year of fee payment: 20

Ref country code: FR

Payment date: 20180511

Year of fee payment: 20

Ref country code: BE

Payment date: 20180515

Year of fee payment: 20

Ref country code: NL

Payment date: 20180514

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: SE

Payment date: 20180612

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20180625

Year of fee payment: 20

Ref country code: GB

Payment date: 20180530

Year of fee payment: 20

Ref country code: ES

Payment date: 20180703

Year of fee payment: 20

REG Reference to a national code

Ref country code: DE

Ref legal event code: R071

Ref document number: 69935978

Country of ref document: DE

REG Reference to a national code

Ref country code: DK

Ref legal event code: EUP

Effective date: 20190601

REG Reference to a national code

Ref country code: NL

Ref legal event code: MK

Effective date: 20190531

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

REG Reference to a national code

Ref country code: GB

Ref legal event code: PE20

Expiry date: 20190531

Ref country code: IE

Ref legal event code: MK9A

Ref country code: BE

Ref legal event code: MK

Effective date: 20190601

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK07

Ref document number: 361360

Country of ref document: AT

Kind code of ref document: T

Effective date: 20190601

REG Reference to a national code

Ref country code: SE

Ref legal event code: EUG

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20190601

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20190531

REG Reference to a national code

Ref country code: ES

Ref legal event code: FD2A

Effective date: 20200904

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20190602