EP1054667B1 - Bizyclische pyrrolverbindungen, deren pharmazeutische zusammensetzungen und ihre verwendung als entzündungshemmende und immunmodulierende mittel - Google Patents

Bizyclische pyrrolverbindungen, deren pharmazeutische zusammensetzungen und ihre verwendung als entzündungshemmende und immunmodulierende mittel Download PDF

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EP1054667B1
EP1054667B1 EP99903807A EP99903807A EP1054667B1 EP 1054667 B1 EP1054667 B1 EP 1054667B1 EP 99903807 A EP99903807 A EP 99903807A EP 99903807 A EP99903807 A EP 99903807A EP 1054667 B1 EP1054667 B1 EP 1054667B1
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alkyl
optionally substituted
compound
group
formula
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French (fr)
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EP1054667A1 (de
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Andrew John Barker
Jason Grant Kettle
Alan Wellington Faull
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AstraZeneca UK Ltd
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AstraZeneca UK Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/52—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring condensed with a ring other than six-membered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/30—Indoles; Hydrogenated indoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to carbon atoms of the hetero ring
    • C07D209/42—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals

Definitions

  • the present invention relates to pharmaceutical compositions which comprise anti-inflammatory and immunomodulatory compounds that act via antagonism of the CCR2 receptor (also known as the MCP-1 receptor), leading inter alia to inhibition of Monocyte Chemoattractant Protein-1 (MCP-1). These compounds contain a bicyclic moiety.
  • the invention further relates to novel compounds for use in the compositions, to processes for their preparation, to intermediates useful in their preparation and to their use as therapeutic agents.
  • MCP-1 is a member of the chemokine family of pro-inflammatory proteins which mediate leukocyte chemotaxis and activation.
  • MCP-1 is a C-C chemokine which is one of the most potent and selective T-cell and monocyte chemoattractant and activating agents known.
  • MCP-1 has been implicated in the pathophysiology of a large number of inflammatory diseases including rheumatoid arthritis, glomerular nephritis, lung fibrosis, restenosis (International Patent Application WO 94/09128), alveolitis (Jones et al., 1992, J. Immunol ., 149 ,2147) and asthma.
  • a CCR2 antagonist may also be useful to treat stroke, reperfusion injury, ischemia, myocardial infarction and transplant rejection.
  • MCP-1 acts through the CCR2 receptor.
  • MCP-2 and MCP-3 may also act, at least in part, through this receptor. Therefore in this specification, when reference is made to "inhibition or antagonism of MCP-1" or “MCP-1 mediated effects” this includes inhibition or antagonism of other cytokine mediated effects including MCP-2 and/or MCP-3 mediated effects when those cytokines are acting through the MCP-1 receptor.
  • EP-A-189690 discloses that inter alia certain cycloalkanopyrrole derivatives can be useful in treating elevated intra-ocular pressure, whilst WO 9730704 suggests that related compounds may be used in treating or preventing macular oedema.
  • RANTES R egulated upon A ctivation, N ormal T -cell E xpressed and S ecreted
  • MCP-1 MCP-1
  • the present invention provides a pharmaceutical composition which comprises a compound of formula (I) or a pharmaceutically acceptable salt, an ester or amide thereof, which are inhibitors of monocyte chemoattractant protein-1; and wherein A and B form an optionally substituted alkylene chain so as to form a ring with the carbon atoms to which they are attached;
  • X is CH 2 or SO 2
  • R 1 is an optionally substituted aryl or heteroaryl ring;
  • R 2 is carboxy, cyano, -C(O)CH 2 OH, -CONHR 4 , -SO 2 NHR 5 , tetrazol-5-yl, SO 3 H, or a group of formula (VI) where R 4 is selected from hydrogen, alkyl, aryl, cyano, hydroxy, -SO 2 R 9 where R 9 is alkyl, aryl, heteroaryl, or haloalkyl, or R 4 is a group-(CHR 10 ) r -COOH where r
  • compositions comprise a compound of formula (I) or a salt or in vivo hydrolysable ester thereof.
  • Example of such compounds are compounds where A, B, X, R 1 and R 3 are as defined above, and where R 2 is as defined above but that R 4 is selected from cyano, hydroxy, -SO 2 R 9 where R 9 is alkyl, aryl, heteroaryl, or haloalkyl, or R 4 is a group-(CHR 10 ) r -COOH where r is an integer of 1-3 and each R 10 group is independently selected from hydrogen or alkyl; R 5 is optionally substituted phenyl or optionally heteroaryl groups, or a group COR 6 where R 6 is alkyl, aryl, heteroaryl or haloalkyl; R 7 and R 8 are independently selected from hydrogen or alkyl, particularly C 1-4 alkyl.
  • Compounds of formula (I) are inhibitors of monocyte chemoattractant protein-1 and therefore can be used to treat inflammatory disease.
  • the invention further provides a compound of formula (I) for use in the treatment of inflammatory disease.
  • the invention provides the use of a compound of formula (I) in the preparation of a medicament for the treatment of inflammatory disease.
  • alkyl' when used either alone or as a suffix includes straight chained, branched structures. These groups may contain up to 10, preferably up to 6 and more preferably up to 4 carbon atoms.
  • alkenyl and alkynyl refer to unsaturated straight or branched structures containing for example from 2 to 10, preferably from 2 to 6 carbon atoms. Cyclic moieties such as cycloalkyl, cycloalkenyl and cycloalkynyl are similar in nature but have at least 3 carbon atoms. Terms such as “alkoxy” comprise alkyl groups as is understood in the art.
  • halo includes fluoro, chloro, bromo and iodo.
  • aryl groups include aromatic carbocylic groups such as phenyl and naphthyl.
  • heterocyclyl includes aromatic or non-aromatic rings, for example containing from 4 to 20, suitably from 5 to 8 ring atoms, at least one of which is a heteroatom such as oxygen, sulphur or nitrogen.
  • Examples of such groups include furyl, thienyl, pyrrolyl, pyrrolidinyl, imidazolyl, triazolyl, thiazolyl, tetrazolyl, oxazolyl, isoxazolyl, pyrazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, quinolinyl, isoquinolinyl, quinoxalinyl, benzothiazolyl, benzoxazolyl, benzothienyl or benzofuryl.
  • Heteroaryl refers to those groups described above which have an aromatic character.
  • aralkyl refers to aryl substituted alkyl groups such as benzyl.
  • hydrocarbyl which refers to any structure comprising carbon and hydrogen atoms.
  • these may be alkyl, alkenyl, alkynyl, aryl, heterocyclyl, alkoxy, aralkyl, cycloalkyl, cycloalkenyl or cycloalkynyl.
  • m is generally an integer from 1-3.
  • R 12 and R 13 together form an optionally substituted ring, the ring will comprise a non-aromatic heterocyclyl group as defined above.
  • Suitable optional substituents for hydrocarbyl groups R 11 , R 12 and R 13 include halo, perhaloalkyl such as trifluoromethyl, mercapto, hydroxy, carboxy, alkoxy, heteroaryloxy, alkenyloxy, alkynyloxy, alkoxyalkoxy, aryloxy (where the aryl group may be substituted by halo, nitro, or hydroxy), cyano, nitro, amino, mono- or di-alkyl amino, oximino (such as hydroxyimino or alkoxyimino) or S(O) m R y where m is as defined above and R y is alkyl.
  • perhaloalkyl such as trifluoromethyl, mercapto, hydroxy, carboxy, alkoxy, heteroaryloxy, alkenyloxy, alkynyloxy, alkoxyalkoxy, aryloxy (where the aryl group may be substituted by halo, nitro, or hydroxy
  • a and B form an alkylene chain which comprises from 3 to 6 carbon atoms so that, together with the carbon atoms to which they are attached, rings of from 5 to 8 atoms result.
  • Suitable substituents for the A-B chain include functional groups as defined above or optionally substituted hydrocarbyl groups or optionally substituted heterocyclic groups. Suitable substituents for these hydrocarbyl or heterocylic groups include those listed above for R 11 , R 12 and R 13 .
  • optionally substituted alkyl such as aralkyl, carboxyalkyl or the amide derivative thereof
  • alkoxy aryloxy; aralkyloxy
  • amino group which is optionally substituted with alkyl, aryl or aralkyl is a group of sub-formula (IV).
  • R 1 is suitably a single aryl ring.
  • R 1 is an optionally substituted phenyl, pyridyl, naphthyl, furyl or thienyl ring.
  • Suitable optional substitutents for R 1 in formula (I) include certain of those listed above for R 11 , R 12 and R 13 other than aryloxy or heteroaryloxy, as well as alkyl, alkenyl, alkynyl.
  • substituents for R 1 include trifluoromethyl, C 1-4 alkyl, halo, trifluoromethoxy, C 1-4 alkoxy, C 1-4 alkanoyl, C 1-4 alkanoyloxy, nitro, carboxy, carbamoyl, C 1-4 alkoxycarbonyl, C 1-4 alkylsulphanyl, C 1-4 alkylsulphinyl, C 1-4 alkylsulphonyl, sulphonamido, carbamoylC 1-4 alkyl, N -(C 1-4 alkyl)carbamoylC 1-4 alkyl, N -(C 1-4 alkyl) 2 carbamoyl-C 1-4 alkyl, hydroxyC 1-4 alkyl or C 1-4 alkoxyC 1-4 alkyl.
  • optional substituents for R 1 are selected from halo, haloalkyl including perhaloalkyl such as trifluoromethyl, carboxy, alkoxy, haloalkoxy, alkenyloxy, alkynyloxy, hydroxyalkoxy, alkoxyalkoxy, alkanoyl, alkanoyloxy, cyano, nitro, amino, mono- or di-alkyl amino, sulphonamido or S(O) m R x where m is as defined above and R x is hydrocarbyl.
  • perhaloalkyl such as trifluoromethyl, carboxy, alkoxy, haloalkoxy, alkenyloxy, alkynyloxy, hydroxyalkoxy, alkoxyalkoxy, alkanoyl, alkanoyloxy, cyano, nitro, amino, mono- or di-alkyl amino, sulphonamido or S(O) m R x where m is as defined above and
  • suitable examples are trifluoromethyl, C 1-4 alkyl, halo, trifluoromethoxy, C 1-4 alkoxy, C 1-4 alkanoyl, C 1-4 alkanoyloxy, nitro, carboxy, C 1-4 alkoxycarbonyl, C 1-4 alkylsulphanyl, C 1-4 alkylsulphinyl, C 1-4 alkylsulphonyl, sulphonamido, hydroxyC 1-4 alkyl or C 1-4 alkoxyC 1-4 alkyl.
  • two such substituents together may form a divalent radical of the formula -O(CH 2 ) 1-4 O- attached to adjacent carbon atoms on the R 1 ring.
  • Preferred substitutents for R 1 are one or more non-polar substituents such as halo.
  • R 1 is substituted by one or more halo groups, in particular chlorine.
  • R 1 groups are 3,4-dichlorophenyl, 3,4-difluorophenyl, 3-fluoro-4-chlorophenyl, 3-chloro-4-fluorophenyl or 2,3-dichloropyrid-5-yl.
  • R 1 is 3,4-dichlorophenyl.
  • R 2 is suitably other than a group SO 2 NH 2 .
  • R 2 examples include carboxy; cyano; tetrazol-5-yl; SO 3 H; -CONHR 4 where R 4 is selected from cyano, hydroxy, -SO 2 R 9 where R 9 is alkyl such as C 1-4 alkyl, aryl such as phenyl, heteroaryl or trifluoromethyl, or R 4 is a group-(CHR 10 ) r -COOH where r is an integer of 1-3 and each R 10 group is independently selected from hydrogen or alkyl such as C 1-4 alkyl; or R 2 is a group -SO 2 NHR 5 where R 5 is optionally substituted phenyl or optionally subtituted 5 or 6 membered heteroaryl groups, or a group COR 6 where R 6 is alkyl such as C 1-4 alkyl, aryl such as phenyl, heteroaryl or trifluoromethyl, or R 2 is a group of formula (VI) where R 7 and R 8 are independently selected from hydrogen or alkyl,
  • R 2 is carboxy or a pharmaceutically acceptable salt or ester thereof, such as a C 1-4 alky ester, and particularly carboxy or a pharmaceutically acceptable salt thereof, especially carboxy.
  • R 3 is an optionally substituted alkyl, alkenyl or alkynyl group, or R 3 is a group which includes an alkyl moiety
  • suitable optional substitutents include halo, perhaloalkyl such as trifluoromethyl, mercapto, hydroxy, carboxy, alkoxy, heteroaryloxy, alkenyloxy, alkynyloxy, alkoxyalkoxy, aryloxy (where the aryl group may be substituted by halo, nitro, or hydroxy), cyano, nitro, amino, mono- or di-alkyl amino, oximino (for example hydroxyimino or alkoxyimino) or S(O) m R y where m is as defined above and R y is alkyl.
  • R 3 is an aryl, aralkyl, aryloxy, cycloalkyl or heterocyclyl group
  • suitable substituents include those as listed above for R 1 .
  • Suitable groups R 3 include hydrogen, fluoro, chloro, bromo, iodo, methyl, cyano, trifluoromethyl, hydroxymethyl, carboxyalkyl, alkoxyalkyl such as C 1-4 alkoxymethyl, methoxy, benzyloxy, carboxyalkoxy such as carboxymethoxy, methylsulphanyl, methylsulphinyl, methylsulphonyl or carboxyC 3-6 cycloalkyl, -(CHR 23 ) r -NR 24 R 25 (where r is 0-2, preferably 1 or 2, each R 23 is independently hydrogen or alkyl, in particular C 1-4 alkyl, R 24 and R 25 are independently selected from H and C 1-4 alkyl or R 24 and R 25 together with the nitrogen to which they are attached form a 5 or 6 membered ring optionally containing one further heteroatom selected from O, N, S, S(O) or SO 2 .
  • R 24 and R 25 together form a heterocylic
  • R 3 include optionally substituted aryl groups such as optionally substituted phenyl group.
  • Suitable substituents for phenyl groups R 3 include one or more groups selected from chlorine, fluorine, methyl, trifluoromethyl, trifluoromethoxy, amino or formyl.
  • R 3 may comprise a range of substituents as listed above, it is preferably hydrogen or a small substituent group such as C 1-4 alkyl in particular methyl, halo or trifluoromethyl, and most preferably hydrogen.
  • X is CH 2 .
  • a preferred class of compounds of formula (I) are those of formula (III) wherein R 1 , R 2 , R 3 and X are as defined in relation to formula (I), Y is a group (CR 18 R 19 ) s , R 14 , R 15 , R 16 , R 17 and each R 18 and R 19 are independently selected from hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, cycloalkynyl or a functional group, and s is an integer of from 1 to 4.
  • R 14 , R 15 , R 16 , R 17 and each R 18 and R 19 are independently selected from hydrogen, alkyl, alkenyl, , alkynyl or a functional group
  • Suitable groups for R 14 , R 15 , R 16 , R 17 and each R 18 and R 19 include hydrogen; trifluoromethyl; C 1-4 alkyl which is optionally substituted for example by aryl, carboxy or amide derivatives thereof; halo; hydroxy; C 1-4 alkoxy; C 1-4 alkanoyl; C 1-4 alkanoyloxy; amino; cyano; C 1-4 alkylamino; di(C 1-4 alkyl)amino; C 1-4 alkanoylamino; nitro; carbamoyl; C 1-4 alkoxycarbonyl; thiol; C 1-4 alkylsulphanyl; C 1-4 alkylsulphinyl; C 1-4 alkylsulphonyl; sulphonamido; alkylsulphonamido, arylsulphonamido, carbamoylC 1-4 alkyl; N -(C 1-4 alkyl)carbamoylC 1-4 al
  • Suitable optional substituents for R 20 and R 21 include those listed above for R 1 .
  • R 14 or R 15 are other than hydrogen.
  • R 14 and R 15 together form an oxo group.
  • R 14 and R 15 together form a NOH or NOR 22 where R 22 has a value as defined above for R*.
  • R 16 , R 17 , R 18 and R 19 are hydrogen.
  • s is 1 or 2.
  • Suitable pharmaceutically acceptable salts of compounds of formula (I) include acid addition salts such as methanesulfonate, fumarate, hydrochloride, hydrobromide, citrate, maleate and salts formed with phosphoric and sulphuric acid.
  • suitable salts are base salts such as an alkali metal salt for example sodium, an alkaline earth metal salt for example calcium or magnesium, an organic amine salt for example triethylamine, morpholine, N -methylpiperidine, N -ethylpiperidine, procaine, dibenzylamine, N , N -dibenzylethylamine or amino acids for example lysine.
  • a preferred pharmaceutically acceptable salt is a sodium salt.
  • An in vivo hydrolysable ester of a compound of the formula (I) containing carboxy or hydroxy group is, for example, a pharmaceutically acceptable ester which is hydrolysed in the human or animal body to produce the parent acid or alcohol.
  • esters for carboxy include alkyl esters, such as C 1-6 alkyl esters for example, ethyl esters, C 1-6 alkoxymethyl esters for example methoxymethyl, C 1-6 alkanoyloxymethyl esters for example pivaloyloxymethyl, phthalidyl esters, C 3-8 cycloalkoxy-carbonyloxyC 1-6 alkyl esters for example 1-cyclohexylcarbonyloxyethyl; 1,3-dioxolen-2-onylmethyl esters for example 5-methyl-1,3-dioxolen-2-onylmethyl; and C 1-6 alkoxycarbonyloxyethyl esters for example 1-methoxycarbonyloxyethyl and may be formed at any carboxy group in the compounds of this invention.
  • alkyl esters such as C 1-6 alkyl esters for example, ethyl esters, C 1-6 alkoxymethyl esters for example methoxymethyl, C 1-6
  • inorganic esters such as phosphate esters and ⁇ -acyloxyalkyl ethers and related compounds which as a result of the in vivo hydrolysis of the ester breakdown to give the parent hydroxy group.
  • ⁇ -acyloxyalkyl ethers include acetoxymethoxy and 2,2-dimethylpropionyloxymethoxy.
  • a selection of in vivo hydrolysable ester forming groups for hydroxy include alkanoyl, benzoyl, phenylacetyl and substituted benzoyl and phenylacetyl, alkoxycarbonyl (to give alkyl carbonate esters), dialkylcarbamoyl and N -(dialkylaminoethyl)- N -alkylcarbamoyl (to give carbamates), dialkylaminoacetyl and carboxyacetyl.
  • Esters which are not in vivo hydrolysable are useful as intermediates in the production of the compounds of formula (I) and therefore these form a further aspect of the invention.
  • a suitable value for an amide includes, for example, a N-C 1-6 alkyl and N,N-di-(C 1-6 alkyl)amide such as N-methyl, N-ethyl, N-propyl, N,N-dimethyl, N-ethyl-N-methyl or N,N-diethylamide.
  • Certain compounds of formula (I) are novel and these form a further aspect of the invention.
  • the invention further provides a compound of formula (I) as defined above, or a pharmaceutically acceptable salt or in vivo hydrolysable ester thereof, which is an inhibitor of monocyte chemoattractant protein-1; with the proviso that where A is -(CH 2 ) 3 -, X is CH 2 , R 2 is carboxy or an ester or amide thereof, and R 3 is hydrogen, R 1 is other than unsubstituted phenyl.
  • Particular and preferred groups of novel compounds include those described above in relation to the pharmaceutical compositions.
  • Some compounds of formula (I) may exist as diastereoisomers and/or may possess chiral centres. It is to be understood that the invention encompasses all such optical isomers and diasteroisomers of compounds of formula (I) and pharmaceutical compositions containing these.
  • the invention further relates to all tautomeric forms of the compounds of formula (I) and pharmaceutical compositions containing these.
  • Suitable leaving groups for Z include halide such as chloride, bromide or iodide, as well as mesylate or tosylate.
  • the reaction is suitably effected in an organic solvent such as dimethylformamide (DMF) tetrahydrofuran (THF) or DCM in the presence of a base such as sodium hydride, sodium hydroxide, potassium carbonate.
  • a suitable phase transfer catalyst such as sodium hydride, sodium hydroxide, potassium carbonate.
  • base and solvent is interdependent to a certain extent in that certain solvents are compatible with some bases only as is understood in the art.
  • sodium hydride may preferably be used with dimethylformamide or tetrahydrofuran and sodium hydroxide is preferably used with dichloromethane and a phase transfer catalyst.
  • the reaction can be carried out at moderate temperatures, for example from 0 to 50°C and conveniently at about ambient temperature.
  • R 2 is an ester group in the compound of formula VII and this may be subsequently converted to an acid or to an amide or to another ester or salt, by conventional methods.
  • X is a group SO 2 and R 2 is a methyl ester of carboxy, it may be converted to the corresponding carboxylic acid by reaction with lithium iodide in dry pyridine or DMF.
  • Compounds of formula (VII) are either known compounds or they may be prepared from known compounds by conventional methods.
  • compounds of formula (VII) may be prepared by reacting a compound of formula (IX) where R 3 is as defined in relation to formula (I), R 28 is a carboxy ester such as an alkyl ester and R 30 is a group which can be removed under reductive conditions such as -NH(C 6 H 5 ); with a cyclic ketone of formula (X) where Q forms a cycloalkyl ring, for example of from 5 to 8 carbon atoms.
  • the reaction in suitably effected in an organic solvent such as organic acids such as acetic acid and propionic acid in the presence of base such as sodium acetate and a reducing agent such as zinc.
  • organic solvent such as organic acids such as acetic acid and propionic acid
  • base such as sodium acetate
  • a reducing agent such as zinc
  • the compounds may be prepared by hydrogenation of the corresponding aromatic compound, such as an appropriately substituted indole.
  • aromatic compound such as an appropriately substituted indole.
  • Hydrogenation may be carried out for example in an organic solvent such as acetic acid and in the presence of a catalyst such as platinum.
  • Substituents on the ring formed by A-B may be introduced either during synthesis as outlined above or using various methods which would be apparent to the skilled person depending upon the nature of the particular substituent to be introduced.
  • one substituent may be changed for a different substituent using conventional chemical methods.
  • reductive amination will convert the oxo substituent to an amine.
  • Amines may be converted to amides by reaction with for example acid halides.
  • Wittig reactions may be used in order to introduce alkyl or substituted alkyl susbtitutents. Other possibilities would be apparent to the skilled person.
  • the compounds are used in methods of treatment of inflammatory disease.
  • a method for antagonising an MCP-1 mediated effect in a warm blooded animal, such as man, in need of such treatment which comprises administering to said animal an effective amount of a compound of formula (I) , or a pharmaceutically acceptable salt, or an in vivo hydrolysable ester thereof.
  • the invention also provides a compound of formula (I) as defined herein, or a pharmaceutically acceptable salt, or an in vivo hydrolysable ester thereof, for use as a medicament.
  • compositions of the invention may be in a form suitable for oral use (for example as tablets, lozenges, hard or soft capsules, aqueous or oily suspensions, emulsions, dispersible powders or granules, syrups or elixirs), for topical use (for example as creams, ointments, gels, or aqueous or oily solutions or suspensions), for administration by inhalation (for example as a finely divided powder or a liquid aerosol), for administration by insufflation (for example as a finely divided powder) or for parenteral administration (for example as a sterile aqueous or oily solution for intravenous, subcutaneous, intramuscular or intramuscular dosing or as a suppository for rectal dosing).
  • oral use for example as tablets, lozenges, hard or soft capsules, aqueous or oily suspensions, emulsions, dispersible powders or granules, syrups or elixir
  • compositions of the invention may be obtained by conventional procedures using conventional pharmaceutical excipients, well known in the act.
  • compositions intended for oral use may contain, for example, one or more colouring, sweetening, flavouring and/or preservative agents.
  • Suitable pharmaceutically acceptable excipients for a tablet formulation include, for example, inert diluents such as lactose, sodium carbonate, calcium phosphate or calcium carbonate, granulating and disintegrating agents such as corn starch or algenic acid; binding agents such as starch; lubricating agents such as magnesium stearate, stearic acid or talc; preservative agents such as ethyl or propyl p -hydroxybenzoate, and anti-oxidants, such as ascorbic acid. Tablet formulations may be uncoated or coated either to modify their disintegration and the subsequent absorption of the active ingredient within the gastrointestinal track, or to improve their stability and/or appearance, in either case, using conventional coating agents and procedures well known in the art.
  • inert diluents such as lactose, sodium carbonate, calcium phosphate or calcium carbonate
  • granulating and disintegrating agents such as corn starch or algenic acid
  • binding agents such as starch
  • Compositions for oral use may be in the form of hard gelatin capsules in which the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules in which the active ingredient is mixed with water or an oil such as peanut oil, liquid paraffin, or olive oil.
  • an inert solid diluent for example, calcium carbonate, calcium phosphate or kaolin
  • water or an oil such as peanut oil, liquid paraffin, or olive oil.
  • Aqueous suspensions generally contain the active ingredient in finely powdered form together with one or more suspending agents, such as sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinyl-pyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents such as lecithin or condensation products of an alkylene oxide with fatty acids (for example polyoxyethylene stearate), or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol mono
  • the aqueous suspensions may also contain one or more preservatives (such as ethyl or propyl p -hydroxybenzoate, anti-oxidants (such as ascorbic acid), colouring agents, flavouring agents, and/or sweetening agents (such as sucrose, saccharine or aspartame).
  • preservatives such as ethyl or propyl p -hydroxybenzoate, anti-oxidants (such as ascorbic acid), colouring agents, flavouring agents, and/or sweetening agents (such as sucrose, saccharine or aspartame).
  • Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil (such as arachis oil, olive oil, sesame oil or coconut oil) or in a mineral oil (such as liquid paraffin).
  • the oily suspensions may also contain a thickening agent such as beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set out above, and flavouring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
  • Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water generally contain the active ingredient together with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients such as sweetening, flavouring and colouring agents, may also be present.
  • the pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions.
  • the oily phase may be a vegetable oil, such as olive oil or arachis oil, or a mineral oil, such as for example liquid paraffin or a mixture of any of these.
  • Suitable emulsifying agents may be, for example, naturally-occurring gums such as gum acacia or gum tragacanth, naturally-occurring phosphatides such as soya bean, lecithin, an esters or partial esters derived from fatty acids and hexitol anhydrides (for example sorbitan monooleate) and condensation products of the said partial esters with ethylene oxide such as polyoxyethylene sorbitan monooleate.
  • the emulsions may also contain sweetening, flavouring and preservative agents.
  • Syrups and elixirs may be formulated with sweetening agents such as glycerol, propylene glycol, sorbitol, aspartame or sucrose, and may also contain a demulcent, preservative, flavouring and/or colouring agent.
  • sweetening agents such as glycerol, propylene glycol, sorbitol, aspartame or sucrose, and may also contain a demulcent, preservative, flavouring and/or colouring agent.
  • compositions may also be in the form of a sterile injectable aqueous or oily suspension, which may be formulated according to known procedures using one or more of the appropriate dispersing or wetting agents and suspending agents, which have been mentioned above.
  • a sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example a solution in 1,3-butanediol.
  • Suppository formulations may be prepared by mixing the active ingredient with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.
  • suitable excipients include, for example, cocoa butter and polyethylene glycols.
  • Topical formulations such as creams, ointments, gels and aqueous or oily solutions or suspensions, may generally be obtained by formulating an active ingredient with a conventional, topically acceptable, vehicle or diluent using conventional procedure well known in the art.
  • compositions for administration by insufflation may be in the form of a finely divided powder containing particles of average diameter of, for example, 30 ⁇ or much less, the powder itself comprising either active ingredient alone or diluted with one or more physiologically acceptable carriers such as lactose.
  • the powder for insufflation is then conveniently retained in a capsule containing, for example, 1 to 50mg of active ingredient for use with a turbo-inhaler device, such as is used for insufflation of the known agent sodium cromoglycate.
  • Compositions for administration by inhalation may be in the form of a conventional pressurised aerosol arranged to dispense the active ingredient either as an aerosol containing finely divided solid or liquid droplets.
  • Conventional aerosol propellants such as volatile fluorinated hydrocarbons or hydrocarbons may be used and the aerosol device is conveniently arranged to dispense a metered quantity of active ingredient.
  • the amount of active ingredient that is combined with one or more excipients to produce a single dosage form will necessarily vary depending upon the host treated and the particular route of administration.
  • a formulation intended for oral administration to humans will generally contain, for example, from 0.5 mg to 2 g of active agent compounded with an appropriate and convenient amount of excipients which may vary from about 5 to about 98 percent by weight of the total composition.
  • Dosage unit forms will generally contain about 1 mg to about 500 mg of an active ingredient.
  • the size of the dose for therapeutic or prophylactic purposes of a compound of the Formula I will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine. As mentioned above, compounds of the Formula I are useful in treating diseases or medical conditions which are due alone or in part to the effects of farnesylation of rats.
  • a daily dose in the range for example, 0.5 mg to 75 mg per kg body weight is received, given if required in divided doses.
  • lower doses will be administered when a parenteral route is employed.
  • a dose in the range for example, 0.5 mg to 30 mg per kg body weight will generally be used.
  • a dose in the range for example, 0.5 mg to 25 mg per kg body weight will be used.
  • Oral administration is however preferred.
  • N,N-Dimethylformamide (DMF) was dried over 4 ⁇ molecular sieves.
  • Anhydrous tetrahydrofuran (THF) was obtained from Aldrich SURESEALTM bottles. Other commercially available reagents and solvents were used without further purification unless otherwise stated.
  • Organic solvent extracts were dried over anhydrous MgSO 4 .
  • 1 H, 13 C and 19 F NMR were recorded on Bruker WM200, WM250, WM300 or WM400 instruments using Me 2 SO-d 6 with Me 4 Si or CCl 3 F as internal standard as appropriate, unless otherwise stated.
  • Example 2 The procedure described in Example 1 was repeated using the appropriate pyrrole and benzyl halide or arylsulfonyl chloride. Thus were obtained the compounds described below.
  • Example 3 The procedure described in Example 3 was repeated using the appropriate pyrrole-2-carboxylic ester. Thus were obtained the compounds described below.
  • Triethyl amine (0.36 mL) was added dropwise to a solution of benzyl 1-(3,4-dichlorobenzyl)-5-formyl-4-oxo-4,5,6,7-tetrahydroindole-2-carboxylate (0.5 g) and p -acetamidobenzenesulphonyl azide (305 mg) at room temperature, and stirred for 18 hours to give a brown solution. The solvent was removed in vacuo to afford a brown oil.
  • Benzyl 4-carboxy-1-(3,4-dichlorobenzyl)cyclopenta[ b ]pyrrole-2-carboxylate (0.1 g) was dissolved in ethyl acetate (5 mL). Palladium on carbon (5 % Pd, 10 mg) was added and the reaction was exposed to an hydrogen atmosphere (1.1 atm) for 4 hours until reaction was complete. The hydrogen was evacuated and the resulting solution was filtered through Celite to remove catalyst. The reaction was concentrated in vacuo to afford the crude product, which was dissolved in a minimum of 2M NaOH, diluted with water (1 mL), and then precipitated by addition of dilute aqueous HCI.
  • Methoxylamine hydrochloride (44 mg) was added to a mixture of ethyl 1-(3,4-dichlorobenzyl)-3-ethyl-4-oxo-4,5,6,7-tetrahydroindole-2-carboxylate (0.15 g) and pyridine (0.2 mL) in ethanol (10 mL) at ambient temperature and the reaction stirred for 18 hours. The reaction was partitioned between 2N hydrochloric acid and ethyl acetate.
  • Example 14 The procedure described in Example 14 was repeated using the appropriate hydroxylamine and ketone. Thus were obtained the compounds described below.
  • Example 16 The procedure of Example 16 was repeated using the appropriate alkyl halide. Thus were obtained the compounds described below.
  • Example 3 The procedure described in Example 3 was repeated using the appropriate 4,5,6,7-tetrahydroindole-2-carboxylate. Thus were obtained the compounds described below.
  • the MCP-1 receptor B (CCR2B) cDNA was cloned by PCR from THP-1 cell RNA using suitable oligonucleotide primers based on the published MCP-1 receptor sequences (Charo et al ., 1994, Proc. Natl. Acad. Sci. USA, 91 , 2752). The resulting PCR products were cloned into vector PCR-IITM (InVitrogen, San Diego, CA.). Error free CCR2B cDNA was subcloned as a Hind III-Not I fragment into the eukaryotic expression vector pCDNA3 (In Vitrogen) to generate pCDNA3/CC-CKR2A and pCDNA3/CCR2B respectively.
  • Linearised pCDNA3/CCR2B DNA was transfected into CHO-K1 cells by calcium phosphate precipitation (Wigler et al ., 1979, Cell , 16 , 777). Transfected cells were selected by the addition of Geneticin Sulphate (G418, Gibco BRL) at 1mg/ml, 24 hours after the cells had been transfected. Preparation of RNA and Northern blotting were carried out as described previously (Needham et at ., 1995, Prot. Express. Purifrc ., 6 , 134). CHO-K1 clone 7 (CHO-CCR2B) was identified as the highest MCP-1 receptor B expressor.
  • CHO-CCR2B cells were grown in DMEM supplemented with 10% foetal calf serum, 2 mM glutamine, 1x Non-Essential Amino Acids, 1x Hypoxanthine and Thymidine Supplement and Penicillin-Streptomycin (at 50 ⁇ g streptomycin/ml, Gibco BRL).
  • Membrane fragments were prepared using cell lysis/differential centrifugation methods as described previously (Siciliano et al ., 1990, J . Biol. Chem ., 265 , 19658). Protein concentration was estimated by BCA protein assay (Pierce, Rockford, Illinois) according to the manufacturer's instructions.
  • 125 I MCP-1 was prepared using Bolton and Hunter conjugation (Bolton et al., 1973, Biochem. J ., 133 , 529; Amersham International plc]. Equilibrium binding assays were carried out using the method of Ernst et al ., 1994, J. Immunol ., 152 , 3541. Briefly, varying amounts of 125 I-labeled MCP-1 were added to 10 mg of purified CHO-CCR2B cell membranes in 100 ml of Binding Buffer. After 1 hour incubation at room temperature the binding reaction mixtures were filtered and washed 5 times through a plate washer (Packard Harvester FiltermateTM 196).
  • Test compounds dissolved in DMSO (5 ⁇ l) were tested in competition with 100 pM labelled MCP-1 over a concentration range (0.1-200 ⁇ M) in duplicate using eight point dose-response curves and IC 50 concentrations were calculated.
  • Compounds tested of the present invention had IC 50 values of less than 5 ⁇ M in the hMCP-1 receptor binding assay described herein.
  • the compound of example 4a had an IC 50 of 0.4 ⁇ M.
  • the human monocytic cell line THP-1 was grown in a synthetic cell culture medium RPMI 1640 supplemented with 10 % foetal calf serum, 2 mM glutamine and Penicillin-Streptomycin (at 50 ⁇ g streptomycin/ml, Gibco BRL). THP-1 cells were washed in HBSS (lacking Ca 2+ and Mg 2+ ) + 1 mg/ml BSA and resuspended in the same buffer at a density of 3 x 10 6 cells/ml. The cells were then loaded with 1 mM FURA-2/AM for 30 min at 37°C, washed twice in HBSS, and resuspended at 1x10 6 cells/ml.
  • THP-1 cell suspension (0.9 ml) was added to a 5 ml disposable cuvette containing a magnetic stirrer bar and 2.1 ml of prewarmed (37°C) HBSS containing 1 mg/ml BSA, 1 mM MgCl 2 and 2 mM CaCl 2 .
  • the cuvette was placed in a fluorescence spectrophotometer (Perkin Elmer, Norwalk, CT) and preincubated for 4 min at 37 ° C with stirring. Fluorescence was recorded over 70 sec and cells were stimulated by addition of hMCP-1 to the cuvette after 10 sec.
  • [Ca 2+ ]i was measured by excitation at 340 nm and 380 nm alternately and subsequent measurement of the intensity of the fluorescence emission at 510 nm.
  • the ratio of the intensities of the emitted fluorescent light following excitation at 340 nm and 380 nm, (R), was calculated and displayed to give andestimateofcytoplasmic[Ca 2+ ] according to the equation:- [Ca 2+ ]i K d (R-Rmin) (Rmax-R) (Sf2/Sb2) where the K d for FURA-2 Ca 2+ complex at 37°C was taken to be 224 nm.
  • R max is the maximal fluorescence ratio determined after addition of 10 mM Ionomycin
  • R min is the minimal ratio determined by the subsequent addition of a Ca 2+ free solution containing 5 mM EGTA
  • Sf2/Sb2 is the ratio of fluorescence values at 380 nm excitation determined at R min and R max , respectively.
  • Chemoattractants were introduced into a 96-well microtiter plate which forms the lower well of a chemotaxis chamber fitted with a PVP-free 5 ⁇ m poresize polycarbonate adhesive framed filter membrane (NeuroProbe MB series, Cabin John, MD 20818, USA) according to the manufacturer's instructions.
  • the chemoattractant was diluted as appropriate in synthetic cell culture medium, RPMI 1640 (Gibco) or supplemented with 2 mM glutamine and 0.5% BSA, or alternatively with HBSS with Ca2+ and Mg2+ without Phenol Red (Gibco) plus 0.1% BSA.
  • THP-1 cells 5x10 5 in 100 ⁇ l RPMI 1640 + 0.5%BSA
  • the chemoattractant was kept at a constant submaximal concentration determined previously for each chemokine (1nM for MCP-1 and 2nM for RANTES) and added to the lower well together with the test compounds dissolved in DMSO (final DMSO concentration ⁇ 0.05% v/v) at varying concentrations.
  • the chamber was incubated for 2 h at 37°C under 5 % CO 2 .
  • the medium was removed from the upper wells which were then washed out with 200 ⁇ l physiological saline before opening the chamber, wiping dry the membrane surface and centrifuging the 96-well plate at 600 g for 5 min to harvest the cells.
  • Supernatant 150 ⁇ l was aspirated and 10 ⁇ l of cell proliferation reagent, WST-1, ⁇ 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-phenyl disulfonate ⁇ plus an electron coupling reagent (Boehringer Mannheim, Cat.no. 1644 807) was added back to the wells.
  • the plate was incubated at 37°C for 3 h and the absorbance of the soluble formazan product was read on a microtitre plate reader at 450 nm.
  • the data was input into a spreadsheet, corrected for any random migration in the absence of chemoattractant and the average absorbance values, standard error of the mean, and significance tests were calculated.
  • hMCP-1 induced concentration dependent cell migration with a characteristic biphasic response, maximal 0.5-1.0 nm.
  • fluorescently tagged cells can be used in order to assist in end point detection.
  • the THP-1 cells used are fluorescently tagged by incubation in the presence of 5mM Calcein AM (Glycine, N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]]-bis[(acetyloxy)methyl] ester; Molecular Probes) for 45 minutes in the dark.
  • 5mM Calcein AM Glycine, N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis
  • Cells are harvested by centrifugation and resuspended in HBSS (without Phenol Red) with Ca2+, Mg2+ and 0.1% BSA. 50ml (2x105 cells) of the cell suspension are placed on the filter above each well and, as above, the unit is incubated at 37°C for 2 hours under 5% CO2. At the end of the incubation, cells are washed off the upper face of the filter with phosphate buffered saline, the filter removed from the plate and the number of cells attracted to either the underside of the filter or the lower well estimated by reading fluorescence at 485nm excitation, 538nm emission wavelengths (fmax, Molecular Devices). The data was input into a spreadsheet, corrected for any random migration in the absence of chemoattractant and the average fluorescence values, standard error of the mean, percentage inhibition and IC50 of compounds under test and significance tests can be calculated.
  • Compound X in the above formulation may comprise a compound illustrated in Examples 1 to 18 herein, for Example, the compounds of Examples 3, 4, 5, 13 and 18.
  • the above formulations may be obtained by conventional procedures well known in the pharmaceutical art.
  • the tablets (a)-(c) may be enteric coated by conventional means, for example to provide a coating of cellulose acetate phthalate.
  • the aerosol formulations (h)-(k) may be used in conjunction with standard, metered dose aerosol dispensers, and the suspending agents sorbitan trioleate and soya lecithin may be replaced by an alternative suspending agent such as sorbitan monooleate, sorbitan sesquioleate, polysorbate 80, polyglycerol oleate or oleic acid.

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Claims (10)

  1. Pharmazeutische Zusammensetzung, enthaltend eine Verbindung der Formel (I)
    Figure 00460001
    oder ein pharmazeutisch unbedenkliches Salz, einen pharmazeutisch unbedenklichen Ester oder ein pharmazeutisch unbedenkliches Amid davon, bei der bzw. dem es sich um einen Inhibitor des Monocyte Chemoattractant Protein-1 handelt und worin
    A und B eine gegebenenfalls substituierte Alkylenkette und somit mit den Kohlenstoffatomen, an die sie gebunden sind, einen Ring bilden;
    X für CH2 oder SO2 steht;
    R1 für einen gegebenenfalls substituierten Aryloder Heteroarylring steht;
    R2 für Carboxy, Cyano, -C(O)CH2OH, -CONHR4, -SO2NHR5, Tetrazol-5-yl, SO3H oder eine Gruppe der Formel (VI)
    Figure 00470001
    worin R4 unter Wasserstoff, Alkyl, Aryl, Cyano, Hydroxy oder -SO2R9, worin R9 für Alkyl, Aryl, Heteroaryl oder Halogenalkyl steht, ausgewählt ist oder eine Gruppe - (CHR10)r-COOH, worin r eine ganze Zahl von 1-3 bedeutet und jede Gruppe R10 unabhängig voneinander unter Wasserstoff oder Alkyl ausgewählt ist, bedeutet; R5 Wasserstoff, Alkyl, gegebenenfalls substituiertes Aryl, wie gegebenenfalls substituiertes Phenyl, oder gegebenenfalls substituiertes Heteroaryl, wie 5-oder 6-gliedrige Heteroarylgruppen, oder eine Gruppe COR6, worin R6 für Wasserstoff, Alkyl, Aryl, Heteroaryl oder Halogenalkyl steht, bedeutet; R7 und R8 unabhängig voneinander unter Wasserstoff und Alkyl, insbesondere C1-4-Alkyl, ausgewählt sind, steht und
    R3 für Wasserstoff, eine funktionelle Gruppe, gegebenenfalls substituiertes Alkyl, gegebenenfalls substituiertes Alkenyl, gegebenenfalls substituiertes Alkinyl, gegebenenfalls substituiertes Aryl, gegebenenfalls substituiertes Heterocyclyl, gegebenenfalls substituiertes Alkoxy, gegebenenfalls substituiertes Aralkyl, gegebenenfalls substituiertes Aralkyloxy oder gegebenenfalls substituiertes Cycloalkyl steht; in Kombination mit einem pharmazeutisch unbedenklichen Träger.
  2. Zusammensetzung nach Anspruch 1, bei der es sich bei der Verbindung der Formel (I) um eine Verbindung der Formel (III)
    Figure 00480001
    worin R1, R2, R3 und X die in Anspruch 1 angegebene Bedeutung besitzen, Y für eine Gruppe (CR18R19)s steht, R14, R15, R16, R17 und jedes R18 und R19 unabhängig voneinander unter Wasserstoff, Alkyl, Cycloalkyl, Alkenyl, Cycloalkenyl, Alkinyl, Cycloalkinyl oder einer funktionellen Gruppe ausgewählt sind und s für eine ganze Zahl von 1 bis 4 steht, handelt.
  3. Zusammensetzung nach Anspruch 2, bei der in der Verbindung der Formel (I) R14, R15, R16, R17 und jedes R18 und R19 unabhängig voneinander unter Wasserstoff; Trifluormethyl; gegebenenfalls durch Aryl, Carboxy oder Amidderivate davon substituiertem C1-4-Alkyl; Halogen; Hydroxy; C1-4-Alkoxy; C1-4-Alkanoyl; C1-4-Alkanoyloxy; Amino; Cyano; C1-4-Alkylamino; Di(C1-4-alkyl)amino; C1-4-Alkanoylamino; Nitro; Carbamoyl; C1-4-Alkoxycarbonyl; Thiol; C1-4-Alkylsulfanyl; C1-4-Alkylsulfinyl; C1-4-Alkylsulfonyl; Sulfonamido; Alkylsulfonamido; Arylsulfonamido; Carbamoyl-C1-4alkyl; N-(C1-4-Alkyl)carbamoyl-C1-4-alkyl; N-(C1-4-Alkyl)2-carbamoyl-C1-4-alkyl; Hydroxy-C1-4-alkyl; C1-4-Alkoxy-C1-4-alkyl; Morpholino; Thiomorpholino; Oxythiomorpholino; Pyrrolidinyl; Carboxy-C1-4-alkylamino; R20; NHR21 und -OR21, worin R20 und R21 unabhängig voneinander unter gegebenenfalls substituiertem Phenyl oder einem gegebenenfalls substituierten 5- oder 6-gliedrigen Heteroarylring ausgewählt sind; ausgewählt ist; oder R14 und R15, R16 und R17 und/oder R18 und R19 gemeinsam eine Oxogruppe oder eine Gruppe =NOR22, worin R22 für Wasserstoff oder eine gegebenenfalls substituierte Hydrocarbylgruppe steht, bilden; mit der Maßgabe, daß R14 und R15 oder R16 und R17 oder R18 und die an das gleiche Kohlenstoffatom gebundene Gruppe R19 nicht beide für Hydroxy, C1-4-Alkoxy, Amino, Cyano, Nitro oder Thiol stehen.
  4. Zusammensetzung nach einem der vorhergehenden Ansprüche, bei der X für CH2 steht.
  5. Zusammensetzung nach einem der vorhergehenden Ansprüche, bei der R1 für einen gegebenenfalls substituierten Phenyl-, Naphthyl-, Furyl- oder Thienylring steht.
  6. Zusammensetzung nach einem der vorhergehenden Ansprüche, bei der R2 für Carboxy oder ein pharmazeutisch unbedenkliches Salz oder einen pharmazeutisch unbedenklichen Ester davon steht.
  7. Zusammensetzung nach Anspruch 2, bei der es sich bei der Verbindung der Formel (I) um eine Verbindung der Formel (III) handelt, in welcher R2 für Carboxy steht, X und R1 die in Anspruch 1 angegebene Bedeutung besitzen, R3 für Wasserstoff oder C1-4-Alkyl steht, s die in Anspruch 2 angegebene Bedeutung besitzt, R14 und R15 unter Wasserstoff, =O, =NOH, =NOR*, worin R* Methyl, Benzyl, Carboxybenzyl, Methoxycarbonylbenzyl, 3-(Carboxy)propyl oder einen Ester davon, wie den Ethylester, 4-Carboxybutyl oder einen Ester davon, wie den Ethylester, und Carboxymethyl bedeutet, ausgewählt sind und R16, R17, R18 und R19 alle für Wasserstoff stehen; oder ein pharmazeutisch unbedenkliches Salz oder ein in vivo hydrolysierbarer Ester davon.
  8. Verbindungen der Formel (I) nach Anspruch 1 zur Verwendung bei der Behandlung einer entzündlichen Erkrankung.
  9. Verbindung der Formel (I) nach Anspruch 1 oder ein pharmazeutisch unbedenkliches Salz oder ein in vivo hydrolysierbarer Ester davon mit der Maßgabe, daß für den Fall, daß A -(CH2)3- bedeutet, X für CH2 steht, R2 für Carboxy oder einen Ester oder ein Amid davon steht, R3 für Wasserstoff steht und R1 nicht für unsubstituiertes Phenyl steht.
  10. Verfahren zur Herstellung einer Verbindung nach Anspruch 9, bei dem man eine Verbindung der Formel (VII)
    Figure 00500001
    worin A, B, R2 und R3 die in Anspruch 1 angegebene Bedeutung besitzen, mit einer Verbindung der Formel (VIII) R1-X-Z worin R1 und X die in Anspruch 1 angegebene Bedeutung besitzen und Z für eine Abgangsgruppe steht, umsetzt und gegebenenfalls danach:
    (i) die Gruppe R2 in eine andere derartige Gruppe umwandelt und/oder
    (ii) an den Gruppen A-B einen Substituenten einführt oder verändert und/oder
    (iii) die Gruppe R3 in eine andere derartige Gruppe umwandelt.
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US6291507B1 (en) 2001-09-18
KR20010040996A (ko) 2001-05-15
ATE237327T1 (de) 2003-05-15
NO20004090D0 (no) 2000-08-16
CN1291095A (zh) 2001-04-11
GB9803226D0 (en) 1998-04-08
BR9907962A (pt) 2000-10-24
DE69906940D1 (de) 2003-05-22
NO20004090L (no) 2000-10-16
AU745772B2 (en) 2002-03-28
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AU2432799A (en) 1999-08-30
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