EP1052497B1 - Dispositif pour fixer des lames de verre - Google Patents

Dispositif pour fixer des lames de verre Download PDF

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Publication number
EP1052497B1
EP1052497B1 EP00303853A EP00303853A EP1052497B1 EP 1052497 B1 EP1052497 B1 EP 1052497B1 EP 00303853 A EP00303853 A EP 00303853A EP 00303853 A EP00303853 A EP 00303853A EP 1052497 B1 EP1052497 B1 EP 1052497B1
Authority
EP
European Patent Office
Prior art keywords
glass
cover glass
mounting medium
drop
slide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP00303853A
Other languages
German (de)
English (en)
Other versions
EP1052497A3 (fr
EP1052497A2 (fr
Inventor
Takayoshi c/o K.K. Tiyoda Seisakusho Sakai
Shinji c/o K.K. Tiyoda Seisakusho Tokudaiji
Takashi c/o K.K. Tiyoda Seisakusho Yoshiike
Kiyokazu c/o K.K. Tiyoda Seisakusho Iijima
Seiichi c/o K.K. Tiyoda Seisakusho Takizawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sakura Seiki Co Ltd
Original Assignee
Sakura Seiki Co Ltd
Sakura Finetek Japan Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sakura Seiki Co Ltd, Sakura Finetek Japan Co Ltd filed Critical Sakura Seiki Co Ltd
Publication of EP1052497A2 publication Critical patent/EP1052497A2/fr
Publication of EP1052497A3 publication Critical patent/EP1052497A3/fr
Application granted granted Critical
Publication of EP1052497B1 publication Critical patent/EP1052497B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
    • Y10T156/17—Surface bonding means and/or assemblymeans with work feeding or handling means
    • Y10T156/1702—For plural parts or plural areas of single part
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
    • Y10T156/17—Surface bonding means and/or assemblymeans with work feeding or handling means
    • Y10T156/1702—For plural parts or plural areas of single part
    • Y10T156/1744—Means bringing discrete articles into assembled relationship
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
    • Y10T156/17—Surface bonding means and/or assemblymeans with work feeding or handling means
    • Y10T156/1702—For plural parts or plural areas of single part
    • Y10T156/1744—Means bringing discrete articles into assembled relationship
    • Y10T156/1751—At least three articles
    • Y10T156/1761—Stacked serially
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
    • Y10T156/17—Surface bonding means and/or assemblymeans with work feeding or handling means
    • Y10T156/1785—Magazine stack directly contacting work

Definitions

  • the present invention relates to a cover glass adhering device, more precisely relates to a cover glass adhering device, which mounts a cover glass onto a mounting medium covering a microscope specimen on a slide glass.
  • a method of making a microscope specimen e.g., a tissue slice of an affected part of a patient, is shown in Fig. 17.
  • the method comprising the steps of: slicing a tissue of the affected part; mounting the tissue slice 10 onto a slide glass 12; removing fat from the tissue slice 10; dyeing the tissue slice 10; dropping a mounting medium, which includes a solvent, onto the tissue slice 10 on the slide glass 12; and mounting a cover glass 16 onto a mounting medium pattern 140, which is formed by dropping the mounting medium along the tissue slice 10.
  • the solvent in the mounting medium evaporates, and the mounting medium solidifies, so that the cover glass 16 is adhered on the slide glass 12.
  • a dropping device which automatically drops the mounting medium onto the specimens 10 on the slide glasses 12, was disclosed in the Japanese Patent Gazettes No. 59-157533 and No. 61-66141 .
  • compressed air which is compressed by a compressor 102, is introduced into an upper inner space of a container 100, in which the mounting medium including the solvent is reservoired, via a regulator 106 of a pressurizing pipe 104.
  • the mounting medium in the container 100 is pushed and sent, by the compressed air in the container 100, to a tank 112 via a spouting pipe 108, whose one end is opened in a lower part of the container 100.
  • the mounting medium in the tank 112 is dropped onto the specimen on the slide glass 12 from a drop nozzle 114.
  • Amount of dropping the mounting medium can be controlled by a control valve 110, which opens and closes with regular time separations.
  • a table 116, on which the slide glass 12 is mounted, is slid in a direction of an arrow, so that the mounting medium can be dropped along the specimen 10 and the mounting medium pattern 140 having the prescribed pattern can be formed.
  • the cover glass 16 has a thickness of 0.06-0.25 mm and flat surfaces, so the piled cover glasses 16 are mutually tightly fitted.
  • a device for taking out the uppermost cover glass of the piled cover glasses 16 from a holder container was disclosed in the Japanese Patent Gazette No. 58-30636 .
  • the device is shown in Fig. 19.
  • a press member 204 is pierced through an elevating member 202, which can be moved in the vertical direction, and its lower end 205 is biased downward by a spring 203.
  • a sucking pad 206 is provided to the elevating member 202.
  • the elevating member 202 is moved downward to push one end of the uppermost glass 16a by the lower end 205 of the press member 204, and the other end of the uppermost glass 16a is pulled upward by the sucking member 206 of the elevating member 202.
  • an upper end of the press member 204 is projected upward, against the elasticity of the spring 203, from the elevating member 202.
  • the elevating member 202 is moved upward as shown in Fig. 19. With this action, the other end of the cover glass 16a is pulled upward by the sucking member 206, and the cover glass 16a is bent downward, so that the cover glass 16a can be removed from the piled glasses 16 and taken out from the holder container 200.
  • the device is shown in Fig. 20.
  • a press member 306 is pierced through a holder member 300, which can be moved in the vertical direction, and its lower end 304 is biased downward by a spring 302.
  • a sucking pad 308 is provided to the holder member 300.
  • the holder member 300 is moved downward and toward the slide glass 12, which has been horizontally mounted on a table 310, until the other end of the cover glass 16 contacts an upper face of one end of the slide glass 12. With further moving the holder member 300 downward, the one end of the cover glass 16 is gradually moved toward the upper face of the slide glass 12. With this action, the cover glass 16 pushes the mounting medium pattern 140 toward the other end of the slide glass 12 and tightly fits thereon, so that air bubbles between the slide glass 12 and the cover glass 16 can be pushed out by the cover glass 16.
  • the sucking pad 308 releases the cover glass 16 and the holder member 300 is moved upward, so that the cover glass 12 can be left on the slide glass 12.
  • the inventors of the present invention have studied a cover glass adhering device, which includes a unit for taking out the cover glass and a unit for mounting the cover glass, so as to automatically make a microscope specimen.
  • the sequential steps for adhering the cover glass onto a specimen on a slide glass can be executed automatically.
  • the cover glass adhering device has following disadvantages: it is difficult to always drop fixed amount of mounting medium onto the specimen; it is difficult to always form a mounting medium pattern into a prescribed pattern; the device must be large in size; and a long cover glass cannot be used.
  • the size of the device can be smaller than that of the conventional device, in which the slide glass is slid, by sliding a drop nozzle; and fixed amount of the mounting medium can be supplied by using a proper supply unit for supplying fixed amount of the mounting medium, e.g., a plunger pump, and removing a drop of the mounting medium dangling from said drop nozzle.
  • a proper supply unit for supplying fixed amount of the mounting medium, e.g., a plunger pump, and removing a drop of the mounting medium dangling from said drop nozzle.
  • mounting medium patterns can be always formed into a prescribed pattern by the steps of: stopping a movement of the drop nozzle at a position, at which the drop action of the mounting medium is begun; beginning the drop action thereof; and moving the drop nozzle along the slide glass.
  • a rotational angle of a holding member which holds one end of the uppermost cover glass of a plurality of cover glasses, can be made narrower by slantingly piling the cover glasses in a holder container.
  • the cover glass adhering device of the present invention which mounts a cover glass onto a mounting medium covering a microscope specimen on a slide glass, comprises:
  • cover glass adhering device further includes:
  • cover glass adhering device of the present invention preferably includes:
  • the traveling unit may include:
  • the cover glass adhering device may further comprise a container, in which a solvent for preventing solidification of the mounting medium is reservoired, being located at a position, which is outside of the slide glass and at which a front end of the drop nozzle can enter the container.
  • control section of the dropping means may control the movement of the traveling unit so as to put the front end of the drop nozzle into a container, in which a solvent for preventing solidification of the mounting medium is reservoired, before dropping the mounting medium, while stopping or after the dropping action.
  • the cover glass supplying means may include, as said holding member, a sucking member being capable of sucking the one end of the uppermost glass of the cover glasses, which have been slantingly piled in the holder container; and may further include:
  • control section of the dropping means may control the traveling unit and the supply unit so as to stop the travel of the drop nozzle, which is moved along the slide glass, after stopping the drop action.
  • a supporting section of the rack, in which the slide glasses are accommodated may have a inclined face, with which side faces of the slide glasses come into contact.
  • the cover glass adhering device of the present embodiment of the present invention is shown in Fig. 1.
  • the cover glass adhering device has a transparent cover 22 for opening and closing a body section 20, and the body section 20 has an operating panel 24. Further, a container 26, in which mounting medium is reservoired, is inserted in the body section 20. As shown in Fig. 2, a screen 28, on which operating conditions are shown, start and stop buttons for each operation, a check button, etc. are provided in the operating panel 24.
  • An adjusting dial 30 for adjusting amount of supplying the mounting medium and a speed adjusting dial 32 for adjusting speed of mounting the cover glasses are also provided in the operating panel 24.
  • a basket container 34 in which a plurality of slide glasses on each of which a specimen is mounted are accommodated, and racks 36, which store the slide glasses on each of which the cover glass is adhered, are provided in the body section 20 shown in Fig. 1.
  • a plurality of baskets 38 in each of which a plurality of the slide glasses on which the specimens are mounted are vertically stored, are provided in the basket container 34.
  • the basket container 34 can be moved by a wire 42, which is driven by a motor 40.
  • the racks 36 are capable of moving in the vertical direction, by a motor 37, so as to store the slide glasses in order.
  • supporting sections 44 which support both ends of the slide glasses 12, are formed in slide glass accommodating sections of the racks 36. Front ends 46 of the supporting sections 44 are formed into an L-shape so as to prevent the slide glasses 12 from falling down. Further, inner parts 48 of the supporting sections 44 are slopes.
  • a conveying means 60 which intermittently conveys the slide glasses 12, which is taken from the basket 38 by slide glass take-out means 50, to the racks 36, is provided between the basket container 34 and the racks 36.
  • the slide glass take-out means 50 includes: a motor 54 for vertically moving a member 53, to which a sucking pad 52 for sucking one end of the slide glass 12, which has been vertically stored in the basket 38, is fixed; and a weight 56.
  • a guide rod 55 which is guided by a guide groove 57 formed in an inner face of the body section 20, is provided to the member 53 so as to rotate the sucking pad 52.
  • the sucking pad 52 is rotated so as to horizontally arrange the slide glass 12, whose one end has been sucked by the sucking pad 52 and vertically pulled upward.
  • sucking pad 52 is connected to one end of a hose whose the other end is connected to vacuum means.
  • the slide glass 12 which has been horizontally arranged, is located in the vicinity of the take-out means 50, then mounted onto a recessed first station 62a of a supporting member 62, which is capable of supporting both ends of the slide glass 12.
  • a recessed second station 62b, a recessed third station 62c and a recessed fourth station 62d are further formed, toward the racks 36, with regular separations.
  • the slide glass 12 which is horizontally mounted on the first station 62a is conveyed to the second station 62b, the third station 62c and the fourth station 62d, in order, by a conveying plate 64 of the conveying means 60.
  • a plurality of recesses 64a are formed in an upper face of the conveying plate 64 and arranged from the take-out means 50 side to the racks 36 side.
  • a projected section 65 is downwardly projected from a bottom face of the conveying plate 64.
  • the projected section 65 is connected to a belt 69, which is engaged with a pulley 67, which is directly driven by a motor 66, and free pulleys 68a, 68b and 68c.
  • the projected section 65 is vertically moved by a cam 71, which is driven by a motor 70.
  • the motors 37, 40, 54, 66 and 70 are controlled by a control section 1.
  • the slide glass 12 which has been horizontally mounted on the first station 62a by the take-out means 50, is moved to a position above the supporting member 62 by the conveying plate 64, and the slide glass 12 is horizontally conveyed, in the same state, to a position above the second station 62a, then the slide glass 12 is moved downward, by the conveying plate 64, so that the slide glass 12 can be horizontally mounted on the second station 62b.
  • the conveying plate 64 which has been moved downward, is horizontally moved toward the first station 62a so as to convey the next slide glass 12.
  • the slide glass 12 can be conveyed to the rest stations and stored in the racks 36 in order.
  • the second station 62b is located in the vicinity of a drop nozzle 72 for dropping the mounting medium; the third station 62c is located in the vicinity of a sucking pad 90 for sucking the cover glass.
  • means for dropping the mounting medium includes: the drop nozzle 72; a connecting member 73a, whose one end is connected to the drop nozzle 72; a motor 73, which constitutes a slide unit for horizontally sliding the drop nozzle 72 and which is connected to the other end of the connecting member 73a; and a solenoid 74, which constitutes an elevating unit for vertically moving the drop nozzle 72 and whose rod 74a is connected to a mid part of the connecting member 73a.
  • the motor 73 and the solenoid 74 are controlled by a control section 2.
  • the control section 2 moves the drop nozzle 72 in the horizontal direction and the vertical direction.
  • a plunger pump 76 which is an example of a supply unit for supplying fixed amount of the mounting medium, supplies the mounting medium from the container 26 to the drop nozzle 72.
  • a motor 76b for moving a plunger 76a of the plunger pump 76 is also controlled by the control section 2.
  • the drop nozzle 72 is capable of dropping the mounting medium onto the slide glass 12, which has been horizontally arranged, by horizontally moving in the longitudinal direction of the slide glass 12.
  • the plunger pump 76 is capable of always supplying the fixed amount of the mounting medium.
  • the drop nozzle 72 shown in Fig. 6 is capable of horizontally moving to a position outside of the slide glass 12.
  • a discharge tray 78 and a container 80, in which a solvent for preventing solidification of the mounting medium is reservoired, are located at positions, which are outside of the slide glass 12 and within a moving range of the drop nozzle 72.
  • a removing member 82 which is capable of removing a drop of the mounting medium dangling from the drop nozzle 72, is vertically extended from the discharge tray 78.
  • Check valves 75 and 77 which are capable of preventing reverse flow of the mounting medium, are provided to mid positions of a tube between the container 26 and the drop nozzle 72.
  • a sucking pad 90 for sucking the cover glass and a holder container 84, in which a plurality of cover glasses 16 are piled, are provided in the vicinity of the third station 62c.
  • the holder container 84 is mounted on a container table 85, which is capable of horizontally rotating.
  • the cover glasses 16 are slantingly piled in the holder container 84.
  • the uppermost cover glass 16a is taken out from the holder container 84 by the sucking pad 90, which acts as a holding member.
  • the sucking pad 90 constitutes means for holding and adhering the cover glass 16 (see Fig. 10).
  • the holding-and-adhering means further includes: an arm 86 in which the sucking pad 90 is provided to one end; a motor 87 for rotating the arm 86 about an axis 86a; and a motor 89 for vertically moving an arm member 88, to which the arm 86 is attached. Further, the arm member 88 has a pin 92, which is provided in the vicinity of the sucking pad 90.
  • the motors 87 and 89 are controlled by a control section 3.
  • sucking pad 90 is connected to one end of a hose whose the other end is connected to the vacuum means.
  • the slide glass 12 which is horizontally located on the first station 62a, is conveyed to the second station 62b by the conveying plate 64 so as to drop the mounting medium onto a specimen on the slide glass 12.
  • This action is controlled by the control section 2, which controls the solenoid 74 and the motors 73 and 76b.
  • the drop nozzle 72 drops the mounting medium onto the specimen on the slide glass 12 and is moved, in the longitudinal direction of the slide glass 12, away from the discharge tray 78, which is located outside of the slide glass, by the motor 73, which is provided to the other end of the connecting member 73a whose one end is connected to the drop nozzle 72, and the solenoid 74, which is connected to the mid part of the connecting member 73a (see Fig. 11A).
  • the drop nozzle 72 reaches a predetermined position on the slide glass 12, supplying the mounting medium by the plunger pump 76 and the travel of the drop nozzle 72 in the longitudinal direction of the slide glass 12 are stopped. Then the drop nozzle 72 are moved upward and moved toward the discharge tray 78 (Fig. 11B).
  • the drop nozzle 72 is moved downward again and moved away from the discharge tray 78 (see Fig. 11C).
  • a drop “B" of the mounting medium which is reservoired in the drop nozzle 72, is apt to dangle from a lower end of the drop nozzle 72, which has been stopped. If the drop nozzle 72, which holds the drop “B”, restarts to drop the mounting medium, amount of the mounting medium dropped on the slide glasses 12 are varied. To drop fixed amount of the mounting medium, the lower end of the drop nozzle 72 is passed over the removing member 82, which is vertically extended from the discharge tray 78, so as to make the drop "B” contact the removing member 82 and remove the drop "B” from the drop nozzle 72.
  • the drop nozzle 72 After removing the drop "B", the drop nozzle 72 restarts to drop the mounting medium at a predetermined position on the slide glass 12, so that the fixed amount of the mounting medium can be dropped onto the slide glass 12.
  • Amount of supplying the mounting medium can be adjusted by the adjusting dial 30 of the operating panel 24 (see Fig. 2).
  • the drop "B" of the mounting medium is removed from the lower end of the drop nozzle 72, and the drop action of the drop nozzle 72 is started when the drop nozzle 72 reaches the predetermined position on the slide glass 12.
  • the drop action of the drop nozzle 72 and the travel of the drop nozzle 72, in the longitudinal direction of the slide glass 12 are stopped when the drop nozzle 72 reaches another predetermined position on the slide glass 12. Then the drop nozzle 72 is moved upward.
  • a mounting medium pattern 14' shown in Fig. 13B is sometimes formed.
  • a start end “C” of the pattern 14', at which the drop action is started, is narrow;
  • a stop end “D” of the pattern 14', at which the drop action is stopped, is formed like a wide bump.
  • the pattern shape shown in Fig.13B air is apt to invade into a space between the cover glass 16 and the slide glass 12 when the cover glass 16 is mounted onto the pattern 14'.
  • the pattern 14' is formed. Namely, at the start end "C", the mounting medium is begun to be dropped and traveled simultaneously, so that the shape of the start end "C” is formed narrower.
  • the control section 2 controls the solenoid 74 and the motor 73, which move the drop nozzle 72 in the predetermined direction, and the motor 76b, which drives the plunger pump 76 for sending the mounting medium to the drop nozzle 72, so as to execute the steps of: once stopping the travel of the drop nozzle 72 at the start end, at which the mounting medium is begun to be dropped onto the slide glass 12; beginning to drop the mounting medium; and starting to travel the drop nozzle 72 along the surface of the slide glass 12. With this action, the start end "C" of the mounting medium pattern can be wide as shown in Fig. 13A.
  • control section 2 controls the solenoid 74 and the motors 73 and 76b so as to stop the travel of the drop nozzle 72 after the drop action is stopped. With this action, the stop end "D" of the mounting medium pattern can be narrower as shown in Fig. 13A.
  • the action is controlled by the control section 3, which controls the motors 87 and 89.
  • the arm 86 whose one end holds the sucking pad 90, is moved downward by the motor 89 so as to move the sucking pad 90 and the pin 92 toward the cover glasses piled in the holder container 84 (see Fig. 14A).
  • the sucking pad 90 sucks and holds one end (lower end) of the uppermost cover glass 16a of the piled cover glasses 16, which have been slantingly piled in the holder container (see Fig. 14B).
  • a front end of the pin 92 which is moved downward together with the sucking pad 90, is located above the sucking pad 90 holding the cover glass 16a.
  • the arm 86 is turned by the motor 87 (see Fig. 10) to make the other end of the uppermost cover glass 16a contact an inner face of the holder container 84 and bend the uppermost cover glass 16a (see Fig. 14C).
  • the one end of the bent cover glass 16a is pressed downward by the pin 92, so that the cover glass 16a is deformed into an S-shape. It is difficult for a long cover glass to peel off from the piled cover glasses by merely bending, but the long cover glass can be easily peeled off by deforming into the S-shape. In the case of using short cover glasses which can be peeled off by merely bending, the pin 92 need not contact the one end of the cover glass.
  • the one end of the cover glass 16a which is taken out from the holder container 84, is slantingly held by the sucking pad 90. Then the arm 86 is turned by the motors 87 and 89, so that the other end of the cover glass 16a contacts the upper face of the slide glass 12, which has been mounted on the third station 62c of the supporting member 62, and the one end of the cover glass 16a is gradually moved close to the upper face of the slide glass 12 (see Fig. 15).
  • Moving speed of the arm 86 can be controlled by adjusting rotational speed of the motor 87. The rotational speed may be adjusted by the dial 32 of the operating panel 24 (see Fig. 2).
  • the moving speed of the arm 86 may be gradually changed, on the basis of characteristics of the mounting medium, speed of dropping the mounting medium, etc., by changing the rotational speed of the motor 87 so as to securely discharge air from the space between the cover glass 16a and the slide glass 12.
  • the drop action of the drop nozzle 72 is stopped until the next slide glass 12 is prepared.
  • the solvent in the mounting medium volatilizes, so that the mounting medium in the drop nozzle 72 is apt to solidify and close the drop nozzle 72.
  • the control section 2 enters the lower end of the drop nozzle 72 into the container 80, which is located outside of the slide glass 12 and within the moving range of the drop nozzle 72 and in which the solvent is reservoired (see Fig. 16). If the drop action is stopped for a short time, the lower end of the drop nozzle 72 may be located in an air space between an entrance of the container 80 and solvent surface so as to prevent the solidification of the mounting medium in the drop nozzle 72.
  • the air space is solvent environment.
  • the lower end of the drop nozzle 72 should be dipped in the solvent.
  • the drop nozzle is left in the air while the drop action is stopped, the mounting medium is apt to solidify. Conventionally, the drop nozzle is manually dipped into the solvent, but this work is troublesome for operators.
  • the lower end of the drop nozzle 72 can be automatically entered the solvent container 80.
  • the lower end of the drop nozzle 72 is located in the air space between the entrance of the container 80 and the solvent surface so as to prevent the solidification of the mounting medium in the drop nozzle 72.
  • the replacement which is required in the conventional drop means, is not required, so that useless cost can be removed and working efficiency can be improved.
  • the cover glass adhering device is controlled by the three control sections 1, 2 and 3.
  • the cover glass adhering device of the present invention may be controlled by one control section.
  • cover glass adhering device of the present invention By using the cover glass adhering device of the present invention, fixed amount of the mounting medium can be always supplied to the slide glasses, the cover glasses having various length can be employed, the device can be small in size, and many microscope specimens can be automatically made for a short time.
  • the cover glass adhering device of the present invention may be properly used in hospitals, etc..

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  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (13)

  1. Dispositif pour apposer une lamelle couvre-objet, qui monte une lamelle couvre-objet (16) sur un milieu de montage couvrant un prélèvement à analyser au microscope (10) sur une lame porte-objet (12),
    caractérisé par :
    un élément de soutien (62) qui fournit une station d'application de milieu de montage (62b) au niveau de laquelle une lame porte-objet (12) peut être retenue horizontalement ;
    une buse à goutte (72) pour verser goutte à goutte le milieu de montage sur un prélèvement (10) sur une lame porte-objet (12), lorsque la lame porte-objet est tenue horizontalement au niveau de ladite station (62b) ;
    un élément de retrait (82) pour retirer une goutte (B) du milieu de montage suspendue à ladite buse à goutte (72), ledit élément de retrait (82) étant situé à l'extérieur de la lame porte-objet (12) lorsqu'elle est située au niveau de ladite station (62b) ; et
    une unité de déplacement pour déplacer ladite buse à goutte (72), de laquelle la goutte suspendue (B) du milieu de montage a été retirée par ledit élément de retrait (82), afin que dans la pratique elle passe le long du prélèvement (10) sur la lame porte-objet (12) située au niveau de ladite station (62b), afin de verser goutte à goutte une quantité prescrite du milieu de montage sur le prélèvement (10).
  2. Dispositif pour apposer une lamelle couvre-objet selon la revendication 1, dans lequel ladite unité de déplacement comprend :
    une unité d'élévation pour déplacer ladite buse à goutte (72) dans la direction verticale ; et
    une unité coulissante pour faire coulisser ladite buse à goutte (72) de sorte que dans la pratique elle se déplace le long du prélèvement (10) sur la lame porte-objet (12) située au niveau de ladite station (62b) jusqu'à une position à l'extérieur de la lame porte-objet (12).
  3. Dispositif pour apposer une lamelle couvre-objet selon la revendication 1 ou 2 et
    comprenant en outre un récipient (80), pour contenir un solvant afin d'empêcher la solidification du milieu de montage, ledit réservoir (80) étant situé à une position, qui (a) lorsqu'elle est située au niveau de ladite station (62b) est à l'extérieur de la lame porte-objet (12) ; et (b) est telle qu'une extrémité avant de ladite buse à goutte (72) peut entrer dans ledit récipient (80).
  4. Dispositif pour apposer une lamelle couvre-objet selon l'une quelconque des revendications 1 à 3, comprenant en outre une section de commande (2) pour commander le mouvement de ladite unité de déplacement afin de placer l'extrémité avant de ladite buse à goutte (72) dans un récipient (80) dans lequel un solvant pour empêcher la solidification du milieu de montage est contenu, avant de verser goutte à goutte le milieu de montage, tout en arrêtant ou après l'action de versement goutte à goutte.
  5. Dispositif pour apposer des lamelles couvre-objets selon l'une quelconque des revendications 1 à 4, qui comprend en outre :
    un récipient de support (84) adapté pour contenir des lamelles couvre-objets (16) empilées de façon inclinée ;
    un élément de maintien (90) capable de maintenir une extrémité d'une lamelle supérieure (16a) d'une pile de lamelles couvre-objets (16), lorsque ces dernières ont été empilées de façon inclinée dans ledit récipient de support (84) ;
    une unité rotative pour tourner ledit élément de maintien (90) afin de plier vers le bas la lamelle supérieure (16a) et de la retirer de la lamelle couvre-objet adjacente (16) ; et
    une unité d'entraînement verticale pour déplacer verticalement ledit élément de maintien (90) et ladite unité rotative.
  6. Dispositif pour apposer une lamelle couvre-objet selon la revendication 5,
    comprenant en outre une broche (92) à proximité dudit élément de maintien (90), de sorte que dans la pratique ladite broche (92) pousse vers le bas la lamelle couvre-objet supérieure (16a), qui est pliée vers le bas, afin de déformer la lamelle couvre-objet supérieure (16a) en une forme en S.
  7. Dispositif pour apposer des lamelles couvre-objets, selon la revendication 1 qui comprend en outre :
    des moyens pour déplacer une lame porte-objet (12), lesdits moyens de déplacement (60) étant prévus entre un récipient à corbeille (34), dans lequel dans la pratique une pluralité de lames porte-objets (12), sur chacune desquelles un prélèvement à analyser au microscope (10) est monté, sont placées, et un portoir (36) pour accueillir les lames porte-objets (12) lorsque chacune a reçu une lamelle couvre-objet (16) pour couvrir le prélèvement (10), lesdits moyens de déplacement (60) ayant une plaque de transport (64) qui dans la pratique transporte par intermittence une lame porte-objet (12) tenue horizontalement vers le portoir (36) ;
    des moyens (50) pour sortir la lame porte-objet (12) du récipient à corbeille (34) et placer horizontalement la lame porte-objet (12) sur une position prédéterminée dans la plaque de transport (64) ;
    ladite buse à goutte (72) étant agencée pour verser goutte à goutte le milieu de montage sur le prélèvement (10), sur la lame porte-objet (12) qui a été horizontalement transportée à une position prédéterminée par lesdits moyens de déplacement (60) ;
    une unité d'alimentation (76), qui est capable de fournir la quantité prescrite de milieu de montage à la buse à goutte (72) pour le verser goutte à goutte sur le prélèvement (10) ; et
    une section de commande (2) pour commander ladite unité de déplacement et ladite unité d'alimentation (76) afin d'arrêter le mouvement de ladite buse à goutte (72) à une extrémité de départ, extrémité à laquelle ladite buse à goutte (72) commence à verser goutte à goutte le milieu de montage sur la lame porte-objet (12), et afin de déplacer ladite buse à goutte (72) le long de la lame porte-objet (12) après avoir commencé l'action de versement goutte à goutte ; et
    des moyens pour fournir la lamelle couvre-objet (16), incluant :
    un récipient de support (84) qui, dans la pratique, contient des lamelles couvre-objets (16) empilées de façon inclinée ;
    un élément de maintien (90) capable de maintenir une extrémité d'une lamelle supérieure (16a) des lamelles couvre-objets (16) qui ont été empilées de façon inclinée dans ledit récipient de support (84) ;
    une unité d'extraction qui dans la pratique tourne ledit élément de maintien (90) afin de plier vers le bas la lamelle supérieure (16a) et de la retirer de la lamelle couvre-objet (16) adjacente, puis de sortir la lamelle supérieure (16a) du récipient de support (84) ; et
    une unité de montage qui, dans la pratique, monte progressivement sur le milieu de montage la lamelle couvre-objet (16), dont l'extrémité est maintenue par l'élément de maintien (90), et qui couvre le prélèvement (10) sur la lame porte-objet (12) transportée vers une position prédéterminée, depuis l'autre extrémité vers la première extrémité.
  8. Dispositif pour apposer une lamelle couvre-objet selon la revendication 7,
    dans lequel une section de support (44) du portoir (36) a une face inclinée (48) disposée de sorte que, dans la pratique, les faces latérales des lames porte-objets (12) entrent en contact avec elle.
  9. Dispositif pour apposer une lamelle couvre-objet selon la revendication 7 ou 8,
    dans lequel la section de commande (2) desdits moyens de versement goutte à goutte commande ladite unité de déplacement et ladite unité d'alimentation (76) afin de déplacer ladite buse à goutte (72) le long de la lame porte-objet (12) après avoir arrêté l'action de versement goutte à goutte.
  10. Dispositif pour apposer une lamelle couvre-objet selon l'une quelconque des revendications 7 à 9, dans lequel ladite unité de déplacement comprend :
    une unité d'élévation pour déplacer ladite buse à goutte (72) dans la direction verticale ; et
    une unité coulissante pour faire coulisser ladite buse à goutte (72) le long du prélèvement (10) sur la lame porte-objet (12) jusqu'à une position à l'extérieur de la lame porte-objet (12).
  11. Dispositif pour apposer une lamelle couvre-objet selon l'une quelconque des revendications 7 à 10,
    comprenant en outre un récipient à solvant (80) pour un solvant afin d'empêcher la solidification du milieu de montage, situé à une position qui est hors de la lame porte-objet (12) et à laquelle une extrémité avant de ladite buse à goutte (72) peut entrer dans ledit récipient (80).
  12. Dispositif pour apposer une lamelle couvre-objet selon la revendication 11,
    dans lequel la section de commande (2) desdits moyens de versement goutte à goutte est adaptée pour commander le mouvement de ladite unité de déplacement afin de placer l'extrémité avant de ladite buse à goutte (72) dans ledit récipient à solvant (80), avant de verser goutte à goutte le milieu de montage, tout en arrêtant ou après l'action de versement goutte à goutte.
  13. Dispositif pour apposer une lamelle couvre-objet selon l'une quelconque des revendications 7 à 12,
    dans lequel ledit élément de maintien (90) est un élément d'aspiration (90) capable d'aspirer une extrémité de la lamelle supérieure (16a) ; et lesdits moyens de fourniture de lamelles couvre-objets comprennent en outre :
    une unité rotative pour tourner ledit élément d'aspiration (90) afin que l'autre extrémité de la lamelle supérieure (16a) soit en contact avec une face intérieure du récipient de support (84) et plient la lamelle supérieure (16a) ; et
    une broche prévue à proximité dudit élément d'aspiration (90), de sorte que dans la pratique ladite broche (92) pousse vers le bas la lamelle supérieure (16a), qui est pliée, afin de déformer la lamelle couvre-objet supérieure (16a) en une forme de S.
EP00303853A 1999-05-11 2000-05-08 Dispositif pour fixer des lames de verre Expired - Lifetime EP1052497B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP13034799 1999-05-11
JP13034799 1999-05-11

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EP1052497A2 EP1052497A2 (fr) 2000-11-15
EP1052497A3 EP1052497A3 (fr) 2003-01-02
EP1052497B1 true EP1052497B1 (fr) 2007-07-11

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EP (1) EP1052497B1 (fr)
JP (1) JP4659915B2 (fr)
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US6568447B1 (en) 2003-05-27
EP1052497A3 (fr) 2003-01-02
JP4659915B2 (ja) 2011-03-30
DE60035452T2 (de) 2008-03-13
EP1052497A2 (fr) 2000-11-15
JP2010217936A (ja) 2010-09-30
DE60035452D1 (de) 2007-08-23

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