EP0946737A2 - Herbizidresistente pflanzen - Google Patents

Herbizidresistente pflanzen

Info

Publication number
EP0946737A2
EP0946737A2 EP97910550A EP97910550A EP0946737A2 EP 0946737 A2 EP0946737 A2 EP 0946737A2 EP 97910550 A EP97910550 A EP 97910550A EP 97910550 A EP97910550 A EP 97910550A EP 0946737 A2 EP0946737 A2 EP 0946737A2
Authority
EP
European Patent Office
Prior art keywords
polynucleotide
herbicide
plants
encoding
gst
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97910550A
Other languages
English (en)
French (fr)
Inventor
Paul Anthony Zeneca Agrochemicals THOMPSON
Mary Elizabeth Zeneca Agrochemicals KNIGHT
Ian Zeneca Agrochemicals JEPSON
Paul Graham Zeneca Agrochemicals THOMAS
Timothy Robert Zeneca Agrochemicals HAWKES
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9623248.3A external-priority patent/GB9623248D0/en
Priority claimed from GBGB9625957.7A external-priority patent/GB9625957D0/en
Priority claimed from GBGB9703855.8A external-priority patent/GB9703855D0/en
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Priority to EP06000993A priority Critical patent/EP1728868A2/de
Publication of EP0946737A2 publication Critical patent/EP0946737A2/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088—Glutathione transferase (2.5.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Definitions

  • the invention still further provides plant material which comprises nucleic acid sequences comprising regions encoding at least two proteins capable of conferring upon the material resistance to at least two herbicides, with the provisos that the material that the material does not contain a polynucleotide which encodes a fusion protein comprising only a 5-enol-pyruvyl-3-phosphoshikimate synthetase (EPSPS) and a glutathione S transferase (GST); (ii) that the material does not contain a polynucleotide which comprises only regions encoding superoxide dismutase (SOD) and glutathione S transferase (GST); (iii) that the material does not contain a polynucleotide which comprises only regions encoding GST and phosphinothricin acetyl transferase (PAT); and (iv). that when the plant from which the material is derived is sugar beet, the herbicide resistance or tolerance conferring genes which
  • the invention also includes a method of selectively controlling weeds in a field comprising weeds and crop plants, wherein the crop plants comprise (i) a polynucleotide comprising at least a first region encoding a first protein capable of conferring on a plant, or tissue comprising it, resistance or tolerance to a first herbicide, and a second region encoding a second protein likewise capable of conferring resistance to a second herbicide, with the provisos (i) that the polynucleotide does not encode a fusion protein comprising only a 5- enol-pyruvyl-3-phosphoshikimate synthetase (EPSPS) and a glutathione S transferase (GST); (ii) that the polynucleotide does not comprise only regions encoding superoxide dismutase (SOD) and glutathione S transferase (GST); (iii) that the polynucleotide does not comprise only regions encoding
  • D ⁇ A is isolated and used to transform Agrobacterium tumefaciens LBA4404 to kanamycin resistance again using standard procedures.
  • Potato and tomato tissue is subjected to Agrobacterium-mediated transformation using standard procedures.
  • Transformed shoots are regenerated from kanamycin resistant callus.
  • Shoots are rooted on MS agar containing kanamycin.
  • Surviving rooted explants are re-rooted to provide about 80 kanamycin resistant transformed tobacco plants.
  • the presence of the 4-HPPD gene (using pre-existing EDIT primers) is verified by PCR. A substantial number of PCR positive plants are selected for further analysis.
  • Probes are excised from plasmids, used for Agrobacterium transformation, containing the GST-27 gene by digestion with EcoRI. The probe is labelled
  • This sequence is placed under expression control of an enhanced 35S promoter and ⁇ OS terminator with an Omega fragment being positioned 5' of the protein encoding regions and isolated as an EcoRI Hind III fragment which is cloned into pJRIi to give dicot vector 2.
  • the calli which contain the GST-27 expression cassette are transferred to plant regeneration media and maize plants are recovered.
  • the transformed maize plants are confirmed - by Western blots of total protein extracts from leaves - to constitutively express the GST gene at high levels.
  • Such plants are cross pollinated with an elite maize inbred line and seed is recovered.
  • To confirm enhanced tolerance of the plants to the herbicide acetochlor the said seeds are planted in soil to which has been applied between 2,000 and 8,000 grams per hectare of the herbicide. The seeds are allowed to germinate and grow for 7 days after which time a sample of the resultant seedlings is assessed for damage caused the chemical and compared to the seedlings (if any) which result from non-transgenic seed sown under identical conditions.
  • Plasmid 1 The vector pUBl is digested with Kpnl and a Kpnl -Notl -Kpnl linker inserted, (sequence 5' CAT TTG CGG CCG CAA ATG GTA C 3 - SEQ ID NO. 17).
  • An EcoRl -Notl -EcoRl linker (5' AAT TCA TTT GCG GCC GCA AAT G 3' (SEQ ID No.18) is inserted into the EcoRl site of DVl-pUC.
  • the resulting plasmid is cut with Ncol and the 5' overhang filled using DNA Polymerase 1 Klenow fragment.
  • the linear vector is then digested with Notl and a Notl -blunt fragment isolated.
  • DV3 # 43 B (a glyphosate resistant line comprising the EPSPS and GOX genes - see Example 4) is cross pollinated onto homozygous ET4-208 and vice versa in the usual way. Seed is collected and used in herbicide trials similar to those described above. The tobacco seed is sown in rows in small units the day before treatment. Each compound is formulated in 4% JF5969 (apart from Racer which was a commercial formulation) and sprayed onto the units in the track sprayer at 200 1 ha. The test is assessed at 13, 20 and 27 DAT. Seedlings that are tolerant to bleaching herbicides are transferred after the final assessment into fresh John Innes 111 compost in 3" pots. After two weeks they are subjected to glyphosate herbicide applied at 500 and 800 g/ha. Scoring is performed 14 and 28 DAT. The resultant plants are resistant to both classes of herbicide, and the resistance is inherited in a Mendelian manner.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
  • Enzymes And Modification Thereof (AREA)
EP97910550A 1996-11-07 1997-10-31 Herbizidresistente pflanzen Withdrawn EP0946737A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06000993A EP1728868A2 (de) 1996-11-07 1997-10-31 Herbizidresistente Pflanzen

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB9623248 1996-11-07
GBGB9623248.3A GB9623248D0 (en) 1996-11-07 1996-11-07 Herbicide resistant plants
GBGB9625957.7A GB9625957D0 (en) 1996-12-13 1996-12-13 Improvements in or relating to organic compounds
GB9625957 1996-12-13
GB9703855 1997-02-25
GBGB9703855.8A GB9703855D0 (en) 1997-02-25 1997-02-25 Improvements in or relating to organic compounds
PCT/GB1997/002996 WO1998020144A2 (en) 1996-11-07 1997-10-31 Herbicide resistant plants

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP06000993A Division EP1728868A2 (de) 1996-11-07 1997-10-31 Herbizidresistente Pflanzen

Publications (1)

Publication Number Publication Date
EP0946737A2 true EP0946737A2 (de) 1999-10-06

Family

ID=27268575

Family Applications (2)

Application Number Title Priority Date Filing Date
EP06000993A Withdrawn EP1728868A2 (de) 1996-11-07 1997-10-31 Herbizidresistente Pflanzen
EP97910550A Withdrawn EP0946737A2 (de) 1996-11-07 1997-10-31 Herbizidresistente pflanzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP06000993A Withdrawn EP1728868A2 (de) 1996-11-07 1997-10-31 Herbizidresistente Pflanzen

Country Status (14)

Country Link
EP (2) EP1728868A2 (de)
JP (1) JP2001503625A (de)
KR (1) KR20000053140A (de)
CN (1) CN1236394A (de)
AU (1) AU4789597A (de)
BG (1) BG103462A (de)
BR (1) BR9712695A (de)
CA (1) CA2269666A1 (de)
CZ (1) CZ161699A3 (de)
IL (1) IL129707A0 (de)
NZ (1) NZ335101A (de)
PL (1) PL333131A1 (de)
SK (1) SK60999A3 (de)
WO (1) WO1998020144A2 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6069115A (en) * 1997-11-12 2000-05-30 Rhone-Poulenc Agrochimie Method of controlling weeds in transgenic crops
US6492578B1 (en) 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
ES2281183T3 (es) * 1998-07-10 2007-09-16 Calgene Llc Expresion de genes de tolerancia a herbicida en plastidios vegetales.
EP2319315B2 (de) 1998-08-13 2018-02-21 Bayer CropScience AG Herbizide Mittel für tolerante oder resistente Maiskulturen
DE19836700A1 (de) 1998-08-13 2000-02-17 Hoechst Schering Agrevo Gmbh Herbizide Mittel für tolerante oder resistente Getreidekulturen
DE19836659A1 (de) 1998-08-13 2000-02-17 Hoechst Schering Agrevo Gmbh Herbizide Mittel für tolerante oder resistente Baumwollkulturen
DE19836660A1 (de) 1998-08-13 2000-02-17 Hoechst Schering Agrevo Gmbh Herbizide Mittel für tolerante oder resistente Sojakulturen
DE19836684A1 (de) 1998-08-13 2000-02-17 Hoechst Schering Agrevo Gmbh Herbizide Mittel für tolerante oder resistente Reiskulturen
AU5779499A (en) * 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Organelle targeting sequences
AU2924800A (en) * 1999-03-03 2000-09-21 Syngenta Limited Use of glutathione-s-transferase to increase stress tolerance in plants
US20020079846A1 (en) * 2000-01-18 2002-06-27 Facchini Peter James Novel glutathione-S-transferase nucleic acids and polypeptides and methods of use thereof
MXPA02012804A (es) * 2000-06-27 2003-05-14 Basf Ag Fragmentos de acido nucleico dianobacterial que codifican las proteinas utiles para controlar las caracteristicas de la planta mediante transformacion nuclear o plastoma.
US7083967B1 (en) 2000-06-27 2006-08-01 Basf Corporation Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation
AU2002214158B2 (en) 2000-12-07 2007-01-18 Syngenta Limited Plant derived hydroxy phenyl pyruvate dioxygenases (HPPD) resistant against triketone herbicides and transgenic plants containing these dioxygenases
RU2532104C2 (ru) 2001-10-19 2014-10-27 Сумитомо Кемикал Компани, Лимитед Метаболизирующий гербицид белок, его ген и их применение
JP4779283B2 (ja) * 2001-10-19 2011-09-28 住友化学株式会社 雑草防除剤代謝蛋白質、その遺伝子およびその利用
EP2278017B1 (de) 2002-02-26 2015-03-25 Syngenta Limited Verfahren zur selektiven Herstellung männlich- oder weiblich-steriler Pflanzen
CN1330762C (zh) * 2002-05-10 2007-08-08 北京大学 新的草甘膦耐受型5－烯醇丙酮酰莽草酸－3－磷酸合酶及其编码基因
CA2897475C (en) 2004-04-30 2018-07-10 Dow Agrosciences Llc Novel herbicide resistance genes
JP4720223B2 (ja) * 2004-05-18 2011-07-13 住友化学株式会社 除草活性化合物耐性植物
AU2006308959B2 (en) 2005-10-28 2012-09-06 Corteva Agriscience Llc Novel herbicide resistance genes
US20070154963A1 (en) * 2005-12-30 2007-07-05 Danying Cai Method to determine whether a compound is a cellular GSTpi inhibitor
US7855326B2 (en) 2006-06-06 2010-12-21 Monsanto Technology Llc Methods for weed control using plants having dicamba-degrading enzymatic activity
AU2007257925B2 (en) 2006-06-06 2012-07-05 Monsanto Technology Llc Method for selection of transformed cells
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
EP3290520B1 (de) 2007-03-09 2021-07-14 Monsanto Technology LLC Zubereitung und verwendung von embryoexplantaten aus pflanzen für die transformation
EA021541B9 (ru) * 2007-04-04 2017-04-28 Басф Плант Сайенс Гмбх Рекомбинантный или мутантный полинуклеотид для придания растениям толерантности к гербицидам, ингибирующим ацетогидроксикислотную синтазу (ahas) (варианты), растение, не встречающееся в природе, и его семена, способ борьбы с сорняками, способ получения трансгенного растения, толерантного к соединениям, ингибирующим ahas, способ идентификации или отбора клетки растения, ткани растения или растения или части растения, способ предотвращения нежелательного вегетативного роста и способ идентификации растения
BRPI0811294B8 (pt) * 2007-05-09 2022-06-28 Dow Agrosciences Llc Polinucleotídeo isolado, e método para controle de ervas daninhas em uma área
AR066787A1 (es) * 2007-05-30 2009-09-09 Syngenta Participations Ag Genes del citocromo p450 que confieren resistencia a los herbicidas
EP2573183B1 (de) 2009-01-22 2017-01-04 Syngenta Participations AG Mutierte Hydroxylphenylpyruvat-Dioxygenase-Polypeptide und Verfahren zu ihrer Anwendung
US9175305B2 (en) 2009-01-22 2015-11-03 Syngenta Participations Ag Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
CA2749383A1 (en) 2009-07-10 2011-06-09 Syngenta Participations Ag Novel hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
CA2785208A1 (en) * 2009-12-23 2011-06-30 Bayer Intellectual Property Gmbh Plants tolerant to hppd inhibitor herbicides
ES2659085T3 (es) * 2009-12-23 2018-03-13 Bayer Intellectual Property Gmbh Plantas tolerantes a herbicidas inhibidores de HPPD
BR112012015692A2 (pt) * 2009-12-23 2015-08-25 Bayer Intelectual Property Gmbh Plantas tolerantes a herbicida inibidor de hppds.
CN102946715A (zh) * 2010-01-07 2013-02-27 巴斯福农业有限责任公司阿纳姆(荷兰)韦登斯维尔分公司 耐受除草剂的植物
MY161279A (en) 2011-03-07 2017-04-14 Sumitomo Chemical Co Method for controlling weeds in paddy rice cultivation
CN102776158B (zh) * 2012-06-14 2013-10-23 重庆市农业科学院 一种抗草甘膦EPSP合成酶GmEPSPS-2及其编码基因与应用
MY196630A (en) 2012-09-14 2023-04-23 BASF Agricultural Solutions Seed US LLC Hppd Variants and Methods of use
BR112016020889B1 (pt) 2014-03-11 2022-10-04 BASF Agricultural Solutions Seed US LLC Molécula de ácido nucleico recombinante, célula hospedeira bacteriana, proteína hppd recombinante, uso do ácido nucleico recombinante e produto de base
WO2015187944A2 (en) * 2014-06-06 2015-12-10 Cornell University Compositions and methods for deterring feeding by psyllids
WO2016007347A1 (en) * 2014-07-11 2016-01-14 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
CN104611304B (zh) * 2014-12-22 2017-05-17 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
CN104830845B (zh) * 2015-04-30 2018-10-30 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9878的核酸序列及其检测方法
CN104878094B (zh) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9888的核酸序列及其检测方法
CN104878095B (zh) * 2015-04-30 2018-10-26 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9858的核酸序列及其检测方法
CN104830846B (zh) * 2015-04-30 2018-10-30 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9898的核酸序列及其检测方法
CN104878096B (zh) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9868的核酸序列及其检测方法
CN104878090B (zh) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 用于检测除草剂耐受性玉米植物dbn9877的核酸序列及其检测方法
AU2016319093B2 (en) 2015-09-11 2022-11-03 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
CN105603047A (zh) * 2015-12-31 2016-05-25 中国烟草总公司郑州烟草研究院 一种适用于植物全蛋白提取液的GSTs活性分析方法
WO2017184727A1 (en) 2016-04-21 2017-10-26 Bayer Cropscience Lp Tal-effector mediated herbicide tolerance
US11708565B2 (en) 2017-03-07 2023-07-25 BASF Agricultural Solutions Seesi US LLC HPPD variants and methods of use
WO2019083810A1 (en) 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE FOR 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) INHIBITORS BY NEGATIVE REGULATION OF HPPD EXPRESSION IN SOYBEANS
BR112020008096A2 (pt) 2017-10-24 2020-11-03 Basf Se método para conferir tolerância a um herbicida e planta de soja transgênica
WO2019233349A1 (zh) 2018-06-04 2019-12-12 青岛清原化合物有限公司 突变型对羟苯基丙酮酸双氧化酶、其编码核酸以及应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7705215B1 (en) * 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
DE4003045A1 (de) * 1990-02-02 1991-08-08 Hoechst Ag Virus/herbizidresistenz-gene, verfahren zu ihrer herstellung und ihre verwendung
CA2083948C (en) * 1990-06-25 2001-05-15 Ganesh M. Kishore Glyphosate tolerant plants
JP3173785B2 (ja) * 1990-08-31 2001-06-04 モンサント カンパニー グリホセート耐性５―エノールピルビルシキミ酸―３―ホスフェートシンターゼ
US5290926A (en) * 1990-09-14 1994-03-01 Ciba-Geigy Corporation Isolated DNA Encoding plant histidinol dehydrogenase
ATE184449T1 (de) * 1991-03-12 1999-10-15 Hoechst Schering Agrevo Gmbh Gegen herbizide vom aryloxy-phenoxy- alkancarbonsäuretyp resistenter maiszellen und verfahren zur herstellung herbizidresistenter maiszelllinien
US5491076A (en) * 1993-11-01 1996-02-13 The Texas A&M University System Expression of foreign genes using a replicating polyprotein producing virus vector
US5767373A (en) * 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
US5631152A (en) * 1994-10-26 1997-05-20 Monsanto Company Rapid and efficient regeneration of transgenic plants
GB9515941D0 (en) * 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
EP0821729B1 (de) * 1995-04-20 2006-10-18 Basf Aktiengesellschaft Auf basis ihrer struktur entworfene herbizid resistente produkte
FR2751347B1 (fr) * 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9820144A3 *

Also Published As

Publication number Publication date
PL333131A1 (en) 1999-11-22
AU4789597A (en) 1998-05-29
NZ335101A (en) 2000-11-24
WO1998020144A3 (en) 1998-07-16
BG103462A (en) 2000-01-31
JP2001503625A (ja) 2001-03-21
BR9712695A (pt) 1999-10-19
WO1998020144A2 (en) 1998-05-14
CN1236394A (zh) 1999-11-24
CZ161699A3 (cs) 1999-08-11
KR20000053140A (ko) 2000-08-25
CA2269666A1 (en) 1998-05-14
EP1728868A2 (de) 2006-12-06
IL129707A0 (en) 2000-02-29
SK60999A3 (en) 1999-10-08

Similar Documents

Publication Publication Date Title
WO1998020144A2 (en) Herbicide resistant plants
US20090229006A1 (en) Herbicide resistant plants
US11371055B2 (en) Herbicide resistance genes
US6380463B1 (en) Inducible herbicide resistance
AU2008251445B2 (en) Novel herbicide resistance genes
US8097774B2 (en) Cytochrome P450 genes conferring herbicide resistance
PL190393B1 (pl) Konstrukt chimerowy zawierający gen chimerowy elementarny, wektor, komórka roślinna, roślina, sposób nadawania oporności na herbicydy roślinom, sposób uzyskiwania roślin o tolerancji na wiele herbicydów oraz sposób działania herbicydem na rośliny
AU2013271455B2 (en) Methods of improving the yield of 2,4-D resistant crop plants
CA2321965A1 (en) Method of producing plants which are tolerant or resistant to herbicides
AU5007101A (en) Herbicide resistant plants
MXPA99004200A (en) Herbicide resistant plants
WO2001007592A2 (en) Herbicide resistant plants and methods for the production thereof
US20130260995A1 (en) Methods of improving the yield of 2,4-d resistant crop plants
AU2012261523A1 (en) Novel herbicide resistance genes

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19990607

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

AX Request for extension of the european patent

Free format text: AL PAYMENT 19990607;LT PAYMENT 19990607;LV PAYMENT 19990607;RO PAYMENT 19990607;SI PAYMENT 19990607

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: SYNGENTA LIMITED

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: SYNGENTA LIMITED

17Q First examination report despatched

Effective date: 20040803

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20060404