EP0925366A1 - Procedes de fabrication de composes carotenoides et d'huiles speciales a partir de graines de plantes - Google Patents
Procedes de fabrication de composes carotenoides et d'huiles speciales a partir de graines de plantesInfo
- Publication number
- EP0925366A1 EP0925366A1 EP97938203A EP97938203A EP0925366A1 EP 0925366 A1 EP0925366 A1 EP 0925366A1 EP 97938203 A EP97938203 A EP 97938203A EP 97938203 A EP97938203 A EP 97938203A EP 0925366 A1 EP0925366 A1 EP 0925366A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- gene
- plant
- seed
- carotenoid
- carotenoid biosynthesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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- 108010058731 nopaline synthase Proteins 0.000 description 1
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- 230000035764 nutrition Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- LWTDZKXXJRRKDG-UHFFFAOYSA-N phaseollin Natural products C1OC2=CC(O)=CC=C2C2C1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-UHFFFAOYSA-N 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000010773 plant oil Substances 0.000 description 1
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 235000003441 saturated fatty acids Nutrition 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
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- 239000013589 supplement Substances 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 235000019245 violaxanthin Nutrition 0.000 description 1
- SZCBXWMUOPQSOX-PSXNNQPNSA-N violaxanthin Chemical compound C(\[C@@]12[C@](O1)(C)C[C@H](O)CC2(C)C)=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)/C=C/[C@]1(C(C[C@@H](O)C2)(C)C)[C@]2(C)O1 SZCBXWMUOPQSOX-PSXNNQPNSA-N 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 235000019195 vitamin supplement Nutrition 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 241000228158 x Triticosecale Species 0.000 description 1
- DHNSFMNURMJEQV-OIBMWOCGSA-N zeaxanthin bis(beta-D-glucoside) Chemical compound O([C@@H]1CC(C)=C(C(C1)(C)C)/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)/C=C/C=1C(C[C@@H](CC=1C)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O DHNSFMNURMJEQV-OIBMWOCGSA-N 0.000 description 1
- 239000002478 γ-tocopherol Substances 0.000 description 1
- QUEDXNHFTDJVIY-DQCZWYHMSA-N γ-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-DQCZWYHMSA-N 0.000 description 1
- 239000002446 δ-tocopherol Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Edible Oils And Fats (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention porte sur des méthodes de production de plantes et de graines ayant des compositions altérées de caroténoïde, d'acide gras et de tocophérol. Lesdits procédés sont particulièrement utiles pour augmenter les niveaux de caroténoïde dans des plantes oléagineuses et pour fournir des huiles de graines à haute teneur en acide oléique souhaitables.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2414596P | 1996-08-09 | 1996-08-09 | |
US24145P | 1996-08-09 | ||
PCT/US1997/014035 WO1998006862A1 (fr) | 1996-08-09 | 1997-08-08 | Procedes de fabrication de composes carotenoides et d'huiles speciales a partir de graines de plantes |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0925366A1 true EP0925366A1 (fr) | 1999-06-30 |
Family
ID=21819082
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP97938203A Withdrawn EP0925366A1 (fr) | 1996-08-09 | 1997-08-08 | Procedes de fabrication de composes carotenoides et d'huiles speciales a partir de graines de plantes |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0925366A1 (fr) |
JP (1) | JP2001505409A (fr) |
CN (1) | CN1227609A (fr) |
AU (1) | AU4058497A (fr) |
BR (1) | BR9713462A (fr) |
CA (1) | CA2261577A1 (fr) |
WO (1) | WO1998006862A1 (fr) |
Families Citing this family (48)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6642021B2 (en) * | 1996-03-29 | 2003-11-04 | University Of Maryland | Methods of producing carotenoids by the expression of plant ε-cyclase genes |
US6429356B1 (en) * | 1996-08-09 | 2002-08-06 | Calgene Llc | Methods for producing carotenoid compounds, and specialty oils in plant seeds |
US6653530B1 (en) | 1998-02-13 | 2003-11-25 | Calgene Llc | Methods for producing carotenoid compounds, tocopherol compounds, and specialty oils in plant seeds |
US6600089B1 (en) | 1998-04-24 | 2003-07-29 | E. I. Du Pont De Nemours And Company | Carotenoid biosynthesis enzymes |
AU3749199A (en) * | 1998-04-24 | 1999-11-16 | E.I. Du Pont De Nemours And Company | Carotenoid biosynthesis enzymes |
JP2002525034A (ja) * | 1998-08-05 | 2002-08-13 | サンジーン ゲーエムベーハー ウント コー.カーゲーアーアー | 1−デオキシ−d−キシルロース−5−リン酸シンターゼをコードするdna配列および植物におけるその過剰産生 |
WO2000011199A1 (fr) * | 1998-08-20 | 2000-03-02 | Pioneer Hi-Bred International, Inc. | Compositions et procedes de modification d'un mecanisme d'action metabolique de l'acetyl-coa d'une plante |
IL142041A0 (en) * | 1998-10-07 | 2002-03-10 | Syngenta Participations Ag | Therapeutically active proteins in plants |
US6232530B1 (en) | 1998-11-30 | 2001-05-15 | University Of Nevada | Marigold DNA encoding beta-cyclase |
US6515206B1 (en) | 1998-12-23 | 2003-02-04 | Calgene Llc | Plastid transformation of Brassica |
WO2000049116A1 (fr) * | 1999-02-18 | 2000-08-24 | Calgene Llc | Procede de raffinage d'une huile de graine contenant des micronutriments |
DE19909637A1 (de) * | 1999-03-05 | 2000-09-07 | Peter Beyer | Verfahren zur Verbesserung des agronomischen Wertes und der ernährungsphysiologischen Eigenschaften von Pflanzen |
ATE446370T1 (de) | 1999-04-12 | 2009-11-15 | Monsanto Technology Llc | Öl, das brassicastanol enthält |
JP2002541851A (ja) * | 1999-04-15 | 2002-12-10 | カルジーン エルエルシー | イソプレノイド合成に関与するタンパク質の核酸配列 |
SE9903336D0 (sv) * | 1999-09-17 | 1999-09-17 | Astacarotene Ab | DNA construct and its use |
CA2401991A1 (fr) * | 2000-03-17 | 2001-09-27 | Exelixis Plant Sciences, Inc. | Agents promoteurs de melon destines a l'expression de transgenes dans des plantes |
US6872815B1 (en) | 2000-10-14 | 2005-03-29 | Calgene Llc | Nucleic acid sequences to proteins involved in tocopherol synthesis |
AR028450A1 (es) * | 2000-05-12 | 2003-05-07 | Monsanto Technology Llc | Mctodos para producir compuestos carotenoides y aceites especiales en semillas de plantas |
MXPA02012804A (es) | 2000-06-27 | 2003-05-14 | Basf Ag | Fragmentos de acido nucleico dianobacterial que codifican las proteinas utiles para controlar las caracteristicas de la planta mediante transformacion nuclear o plastoma. |
US7083967B1 (en) | 2000-06-27 | 2006-08-01 | Basf Corporation | Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation |
AU2001290522B2 (en) | 2000-08-07 | 2006-11-30 | Monsanto Technology Llc | Methyl-D-erythritol phosphate pathway genes |
ES2318004T3 (es) | 2001-05-09 | 2009-05-01 | Monsanto Technology Llc | Genes tyra y usos de los mismos. |
WO2003016482A2 (fr) | 2001-08-17 | 2003-02-27 | Monsanto Technology Llc | Genes de methyltransferase et leurs utilisations |
DE60235252D1 (de) | 2001-10-25 | 2010-03-18 | Monsanto Technology Llc | Aromatische methyltransferasen und ihre verwendung |
BR0308740A (pt) | 2002-03-19 | 2007-01-09 | Monsanto Technology Llc | ácidos nucléicos e polipeptìdeos de homogentisado prenil transferase ("hpt"), e empregos destes |
AU2003268083A1 (en) | 2002-08-05 | 2004-02-23 | Monsanto Technology, Llc | Tocopherol biosynthesis related genes and uses thereof |
CA2495444A1 (fr) * | 2002-08-20 | 2004-03-04 | Sungene Gmbh & Co.Kgaa | Procede d'obtention de cetocarotinoides dans des fruits de plantes |
PT1589807E (pt) * | 2002-12-06 | 2012-02-02 | Del Monte Fresh Produce Company | Plantas transgénicas de ananás com níveis modificados de carotenóides e métodos da sua produção |
DE602004011035T2 (de) * | 2003-03-24 | 2008-12-18 | Syngenta Ltd., Guildford | Erhöhte ansammlung von karottenoiden in pflanzen |
DE102004007624A1 (de) * | 2004-02-17 | 2005-09-15 | Sungene Gmbh & Co. Kgaa | Neue Ketolasen und Verfahren zur Herstellung von Ketocarotinoiden |
AU2006287233A1 (en) * | 2005-09-02 | 2007-03-08 | Cornell University | Polynucleotides encoding carotenoid and apocarotenoid biosynthetic pathway |
CN100410376C (zh) * | 2006-03-06 | 2008-08-13 | 中国科学院遗传与发育生物学研究所 | 与油脂代谢调控相关的转录因子GmDofC及其编码基因与应用 |
CA2678762A1 (fr) * | 2007-02-21 | 2008-08-28 | Abdelali Hannoufa | Modification de profils des carotenoides de plantes |
ES2339094B1 (es) * | 2008-11-13 | 2011-03-18 | Iden Carbohydrate Biotechnology, S.L. | Procedimiento para la produccion de plantas transgenicas que presentan alto contenido en compuestos antioxidantes, alta capacidad antioxidante y resistencia al empardecimiento. |
CN101514345B (zh) * | 2009-02-19 | 2012-07-04 | 上海交通大学 | 生菜植醇激酶蛋白编码序列 |
US20140123339A1 (en) | 2012-10-31 | 2014-05-01 | Pioneer Hi Bred International Inc | Transformed Plants Having Increased Beta-Carotene Levels, Increased Half-Life and Bioavailability and Methods of Producing Such |
CN104357479B (zh) * | 2014-11-18 | 2017-06-06 | 西南大学 | 干扰黄色色素表达同时超量表达番茄红素在制备红色花瓣芸薹属植物中的应用 |
CN104480128B (zh) * | 2014-11-18 | 2018-02-27 | 西南大学 | 抑制黄色色素积累同时积累番茄红素和花青素苷在制备花瓣呈红色芸薹属植物中的应用 |
CN104404079B (zh) * | 2014-12-19 | 2017-03-15 | 西南大学 | 干扰lcyb、lcye表达同时超量表达gl3在制备花瓣呈红色芸薹属植物中的应用 |
CN104404080B (zh) * | 2014-12-19 | 2017-06-23 | 西南大学 | 干扰lcyb、lcye表达同时超量表达egl3在制备花瓣呈红色芸薹属植物中的应用 |
CN104531755B (zh) * | 2014-12-19 | 2017-06-06 | 西南大学 | 干扰lcyb、lcye表达同时异位表达tt8在制备红色花瓣芸薹属植物中的应用 |
CN104388464B (zh) * | 2014-12-19 | 2017-04-26 | 西南大学 | 干扰lcyb、lcye表达并超量表达crtiso在制备红色花瓣芸薹属植物中的应用 |
CN104531754B (zh) * | 2014-12-19 | 2017-05-31 | 西南大学 | 干扰lcyb、lcye表达同时超量表达pap在制备花瓣呈红色芸薹属植物中的应用 |
CN105695506A (zh) * | 2016-04-20 | 2016-06-22 | 西南大学 | 一种改良棉籽营养品质的方法及其用途 |
EP3792353A1 (fr) * | 2019-09-13 | 2021-03-17 | CRAG - Centre de Recerca en Agrigenomica CSIC-IRTA-UB-UAB | Biogenèse chromoplaste artificielle |
CN110656114B (zh) * | 2019-10-18 | 2022-07-01 | 云南中烟工业有限责任公司 | 一种烟草色素合成相关的基因及其应用 |
CN111926036B (zh) * | 2020-10-14 | 2020-12-29 | 中国农业科学院生物技术研究所 | 一种联合表达载体及其在玉米籽粒表达虾青素中的应用 |
CN113549639B (zh) * | 2021-07-21 | 2022-07-29 | 云南中烟工业有限责任公司 | 一种降低烟叶总蛋白及烟气苯酚含量的调控基因 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4727219A (en) * | 1986-11-28 | 1988-02-23 | Agracetus | Genic male-sterile maize using a linked marker gene |
US5922602A (en) * | 1988-02-26 | 1999-07-13 | Biosource Technologies, Inc. | Cytoplasmic inhibition of gene expression |
EP0462231A4 (en) * | 1989-08-01 | 1992-05-13 | Pioneer Hi-Bred International, Inc. | Transcriptional activators of anthocyanin biosynthesis as visual markers for plant transformation |
US7705215B1 (en) * | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
GB8928179D0 (en) * | 1989-12-13 | 1990-02-14 | Ici Plc | Dna,constructs,cells and plants derived therefrom |
US5618988A (en) * | 1990-03-02 | 1997-04-08 | Amoco Corporation | Enhanced carotenoid accumulation in storage organs of genetically engineered plants |
DE69132769T2 (de) * | 1990-03-02 | 2002-04-18 | Bp Corp North America Inc | Biosynthese von carotinoiden in genetisch hergestellten wirtszellen |
RU2005352C1 (ru) * | 1991-09-19 | 1994-01-15 | Владимир Савельевич Феденко | Способ определения специфичности эндоспермовых мутантов кукурузы к накоплению каротиноидов |
GB9204583D0 (en) * | 1992-03-03 | 1992-04-15 | Unilever Plc | Recombinant plant enzyme |
EP0668919B1 (fr) * | 1992-11-17 | 2003-06-04 | E.I. Du Pont De Nemours And Company | Genes pour des desaturases d'acides gras en position delta-12 microsomales et enzymes apparentees provenant de plantes |
CA2150133A1 (fr) * | 1993-02-05 | 1994-08-18 | Vincent Jean-Marie Armel Arondel | Plantes dont le contenu en acide linolenique et en acide linoleique a ete modifie |
WO1996002650A2 (fr) * | 1994-07-18 | 1996-02-01 | Zeneca Limited | Adn, produits de recombinaison, cellules et plantes derivees de celles-ci |
-
1997
- 1997-08-08 WO PCT/US1997/014035 patent/WO1998006862A1/fr not_active Application Discontinuation
- 1997-08-08 AU AU40584/97A patent/AU4058497A/en not_active Abandoned
- 1997-08-08 EP EP97938203A patent/EP0925366A1/fr not_active Withdrawn
- 1997-08-08 JP JP50991198A patent/JP2001505409A/ja active Pending
- 1997-08-08 BR BR9713462-7A patent/BR9713462A/pt not_active IP Right Cessation
- 1997-08-08 CN CN 97197150 patent/CN1227609A/zh active Pending
- 1997-08-08 CA CA002261577A patent/CA2261577A1/fr not_active Abandoned
Non-Patent Citations (1)
Title |
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See references of WO9806862A1 * |
Also Published As
Publication number | Publication date |
---|---|
CN1227609A (zh) | 1999-09-01 |
AU4058497A (en) | 1998-03-06 |
JP2001505409A (ja) | 2001-04-24 |
CA2261577A1 (fr) | 1998-02-19 |
BR9713462A (pt) | 2000-03-28 |
WO1998006862A1 (fr) | 1998-02-19 |
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