EP0486073A2 - Liquid detergent composition containing lipase and protease - Google Patents

Liquid detergent composition containing lipase and protease Download PDF

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Publication number
EP0486073A2
EP0486073A2 EP91200149A EP91200149A EP0486073A2 EP 0486073 A2 EP0486073 A2 EP 0486073A2 EP 91200149 A EP91200149 A EP 91200149A EP 91200149 A EP91200149 A EP 91200149A EP 0486073 A2 EP0486073 A2 EP 0486073A2
Authority
EP
European Patent Office
Prior art keywords
detergent composition
protease
composition according
lipase
mixtures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP91200149A
Other languages
German (de)
French (fr)
Other versions
EP0486073B1 (en
EP0486073A3 (en
Inventor
James Pyott Johnston
Pierre Marie Alain Lenoir
Christiaan Arthur Jacques Kamiel Thoen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Procter and Gamble Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26128002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP0486073(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Procter and Gamble Co filed Critical Procter and Gamble Co
Priority to DE1991621059 priority Critical patent/DE69121059T2/en
Priority to ES91200149T priority patent/ES2091280T3/en
Priority to EP19910200149 priority patent/EP0486073B1/en
Priority to DK91200149T priority patent/DK0486073T3/en
Priority to JP4500878A priority patent/JPH06504555A/en
Priority to AU90416/91A priority patent/AU9041691A/en
Priority to CA002096256A priority patent/CA2096256C/en
Priority to PCT/US1991/008041 priority patent/WO1992008779A1/en
Priority to MX9102022A priority patent/MX9102022A/en
Priority to NZ24057191A priority patent/NZ240571A/en
Priority to IE395191A priority patent/IE913951A1/en
Priority to CN 91111550 priority patent/CN1062374A/en
Priority to TR106991A priority patent/TR27423A/en
Priority to TW81100525A priority patent/TW215108B/zh
Publication of EP0486073A2 publication Critical patent/EP0486073A2/en
Publication of EP0486073A3 publication Critical patent/EP0486073A3/en
Priority to US08/322,965 priority patent/US5733473A/en
Publication of EP0486073B1 publication Critical patent/EP0486073B1/en
Application granted granted Critical
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/66—Non-ionic compounds
    • C11D1/83—Mixtures of non-ionic with anionic compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/02—Anionic compounds
    • C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
    • C11D1/14—Sulfonic acids or sulfuric acid esters; Salts thereof derived from aliphatic hydrocarbons or mono-alcohols
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/38—Cationic compounds
    • C11D1/52—Carboxylic amides, alkylolamides or imides or their condensation products with alkylene oxides
    • C11D1/525—Carboxylic amides (R1-CO-NR2R3), where R1, R2 or R3 contain two or more hydroxy groups per alkyl group, e.g. R3 being a reducing sugar rest
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/66—Non-ionic compounds
    • C11D1/72—Ethers of polyoxyalkylene glycols

Definitions

  • the present invention relates to liquid detergent compositions which contain an enzyme system.
  • the enzyme system is a combination of a modified protease and a lipase.
  • detergent compositions may advantageously comprise enzyme systems.
  • enzyme systems include cellulase, protease, lipase and amylases.
  • the present invention is specifically aiming at providing liquid detergent compositions in which the enzyme system comprises a mixture of protease and lipase.
  • liquid detergent composition comprising an enzyme system comprising a lipase and a protease, wherein said enzyme system is stable; by stable, it is meant that the proteolytic degradation of the lipase is substantially reduced.
  • Modified bacterial serine proteases including proteases suitable for use in the compositions according to the invention are disclosed for instance in EP-A-0 328 229 as well as their use in detergent compositions.
  • This patent application describes among others a modified bacterial serine protease which is commercially available from GIST-BROCADES under the name MAXAPEM 15 R
  • LIPOLASE R can be combined with proteases to form a granular enzymatic detergent additive.
  • EP-A-0 381 262 describes detergent compositions comprising a protease and a lipase, preferably LIPOLASE R , together with a stabilizing system.
  • the proteases disclosed in this reference include bacterial proteases.
  • the present invention is a liquid detergent composition
  • a liquid detergent composition comprising an enzyme system, characterized in that the enzyme system comprises a modified bacterial serine protease or mixtures thereof, and a lipase or mixtures thereof.
  • the bacterial serine protease is modified in that the methionine adjacent to the serine of the active site is substituted by another amino acid.
  • the enzyme system according to the present invention comprises a lipase and a protease.
  • Any lipase suitable for use in a detergent composition can be used in the compositions according to the invention, as described for instance in EP 0 381 262 or EP 271 152.
  • the preferred lipase to be used in the compositions according to the present invention is a lipase derived from Humicola lanuginosa , as described in EP-A-0 258 068 to NOVO INDUSTRI A/S. This patent application describes how to obtain said specific lipase, but said specific lipase is also commercially available from NOVO NORDISK A/S under the trade name LIPOLASE R .
  • lipases suitable for use herein are Amono-P Lipase R , Amono-B Lipase R , Amono CES Lipase R , Amono AKG Lipase R , all from Amono Pharmaceuticals, Japan; Toyo Jozo Co, Japan and US biochemical Corp. USA as well as Diosynth Co, NL also commercialize suitable lipases for use in the compositions according to the present invention.
  • compositions according to the present invention typically comprise from 0.1 to 10000 Lipolytic Units per gram of finished product, preferably from 10 to 2500 Lipolytic Units per gram of finished product.
  • Lipolytic units are defined for instance in EP 0 258 268, page 5 line 38..
  • the proteases to be used according to the present invention are modified bacterial serine proteases. All native bacterial serine proteases are characterized in that the active site invariably comprises a triade of amino acids which are serine, histidine and aspartic acid. These amino acids are positioned in the native form of the enzyme in such a way that they catalyse the cleavage of internal peptide bonds of proteins. Another common point between these bacterial serine proteases is that there always is a methionine adjacent to the serine of the active site, in the native sequence.
  • the bacterial serine proteases suitable for use according to the present invention are those wherein the methionine adjacent to the serine of the active site has been substituted by another amino acid.
  • the serine of the active site can also be defined as the serine which is homologuous to the serine in position 221 in the amino acid sequence of the bacterial subtilisin protease produced by Bacillus Subtilis ; said sequence is listed herein after as SEQ ID NO 1
  • SEQ ID NO 1 In the sequence of this bacterial subtilisin protease produced by Bacillus Subtilis , the methionine is immediately after the serine in position 221 and therefore it is the methionine in position 222 which needs to be substituted by another amino acid.
  • this methionine would not be immediately following the serine of the active site; in such a case, it is the methionine homologuous to the methionine in position 222 in the sequence of this bacterial subtilisin protease produced by Bacillus Subtilis which needs to be substituted by another amino acid.
  • modified bacterial serine proteases suitable for use in the compositions according to the invention are commercially available, such as DURAZYM R from NOVO, which is the methionine modified version of SAVINASE R ; another example of available modified protease is MAXAPEM 15 from GIST-BROCADES, which is the modified version of MAXACAL R wherein the methionine in position 216 has been substituted. Also available are experimental samples of modified OPTICLEAN R and OPTIMASE R , from SOLVAY enzymes; both are modified in that the methionine in position 222 is substituted by a cysteine.
  • Preferred modified bacterial serine protease according to the present invention are MAXAPEM 15 R from GIST BROCADES and DURAZYM R from NOVO.
  • compositions according to the present invention typically will contain from 0.005 to 10 mg of active protease per gram of finished product, preferably from 0.01 to 5.0 mg of active protease per gram of finished product. Mixtures of the modified bacterial serine protease described herein above are also suitable for use in the compositions according to the invention.
  • the rest of the liquid detergent composition according to the present invention is made of conventional detergency ingredients, i.e. water, surfactants, builders and others.
  • the following description of these ingredients is for the sake of completeness of the description and is not to be construed as limiting the compositions of the present invention to those conventional ingredients described.
  • the liquid detergent compositions herein comprises from 5% to 60% by weight of the total liquid detergent composition, preferably from 10% by weight to 40% by weight of an organic surface-active agent selected from nonionic, anionic, cationic and zwitterionic surface-active agents and mixtures thereof.
  • Suitable anionic surface-active salts are selected from the group of sulfonates and sulfates.
  • the like anionic surfactants are well-known in the detergent arts and have found wide application in commercial detergents.
  • Preferred anionic water-soluble sulfonate or sulfate salts have in their molecular structure an alkyl radical containing from about 8 to about 22 carbon atoms.
  • Such preferred anionic surfactant salts are the reaction products obtained by sulfating C8-C18 fatty alcohols derived from e.g. tallow oil, palm oil, palm kernel oil and coconut oil; alkylbenzene sulfonates wherein the alkyl group contains from about 9 to about 15 carbon atoms; sodium alkylglyceryl ether sulfonates; ether sulfates of fatty alcohols derived from tallow and coconut oils; coconut fatty acid monoglyceride sulfates and sulfonates; and water-soluble salts of paraffin sulfonates having from about 8 to about 22 carbon atoms in the alkyl chain.
  • Sulfonated olefin surfactants as more fully described in e.g. U.S. Patent Specification 3,332,880 can also be used.
  • the neutralizing cation for the anionic synthetic sulfonates and/or sulfates is represented by conventional cations which are widely used in detergent technology such as sodium, potassium or alkanolammonium.
  • a suitable anionic synthetic surfactant component herein is represented by the water-soluble salts of an alkylbenzene sulfonic acid, preferably sodium alkylbenzene sulfonates, preferably sodium alkylbenzene sulfonates having from about 10 to 13 carbon atoms in the alkyl group.
  • Another preferred anionic surfactant component herein is sodium alkyl sulfates having from about 10 to 15 carbon atoms in the alkyl group.
  • nonionic surfactants suitable for use herein include those produced by condensing ethylene oxide with a hydrocarbon having a reactive hydrogen atom, e.g., a hydroxyl, carboxyl, or amido group, in the presence of an acidic or basic catalyst, and include compounds having the general formula RA(CH2CH2O) n H wherein R represents the hydrophobic moiety, A represents the group carrying the reactive hydrogen atom and n represents the average number of ethylene oxide moieties. R typically contains from about 8 to 22 carbon atoms They can also be formed by the condensation of propylene oxide with a lower molecular weight compound. n usually varies from about 2 to about 24.
  • a preferred class of nonionic ethoxylates is represented by the condensation product of a fatty alcohol having from 12 to 15 carbon atoms and from about 4 to 10 moles of ethylene oxide per mole or fatty - alcohol.
  • Suitable species of this class of ethoxylates include : the condensation product of C12-C15 oxo-alcohols and 3 to 9 moles of ethylene oxide per mole of alcohol; the condensation product or narrow cut C14-C15 oxo-alcohols and 3 to 9 moles of ethylene oxide per mole of fatty(oxo)alcohol; the condensation product of a narrow cut C12-C13 fatty(oxo)alcohol and 6,5 moles of ethylene oxide per mole of fatty alcohol; and the condensation products of a C10-C14 coconut fatty alcohol with a degree of ethoxylation (moles EO/mole fatty alcohol) in the range from 4 to 8.
  • the fatty oxo alcohols while mainly linear can have, depending upon the processing conditions and raw material olefins, a certain degree of branching, particularly short chain such as methyl branching.
  • a degree of branching in the range from 15% to 50% (weight%) is frequently found in commercial oxo alcohols.
  • Suitable cationic surfactants include quaternary ammonium compounds of the formula R1R2R3R4N+ where R1,R2 and R3 are methyl groups, and R4 is a C12 ⁇ 15 alkyl group, or where R1 is an ethyl or hydroxy ethyl group, R2 and R3 are methyl groups and R4 is a C12 ⁇ 15 alkyl group.
  • Zwitterionic surfactants include derivatives of aliphatic quaternary ammonium, phosphonium, and sulfonium compounds in which the aliphatic moiety can be straight or branched chain and wherein one of the aliphatic substituents contains from about 8 to about 24 carbon atoms and another substituent contains, at least, an anionic water-solubilizing group.
  • Particularly preferred zwitterionic materials are the ethoxylated ammonium sulfonates and sulfates disclosed in U.S. Patents 3,925,262, Laughlin et al., issued December 9, 1975 and 3,929,678, Laughlin et al., issued December 30, 1975.
  • Semi-polar nonionic surfactants include water-soluble amine oxides containing one alkyl or hydroxy alkyl moiety of from about 8 to about 28 carbon atoms and two moieties selected from the group consisting of alkyl groups and hydroxy alkyl groups, containing from 1 to about 3 carbon atoms which can optionally be joined into ring structures.
  • Poly hydroxy fatty acid amide surfactants of the formula R2-C-N-Z, wherein R1 is H, O R1 C1 ⁇ 4hydrocarbyl, 2-hydroxy ethyl, 2-hydroxy propyl or a mixture thereof, R2 is C5 ⁇ 31 hydrocarbyl, and Z is a polyhydroxyhydrocarbyl having a linear hydrocarbyl chain with at least 3 hydroxyls directly connected to the chain, or an alkoxylated derivative thereof.
  • R1 is methyl
  • R2 is a straight C11 ⁇ 15 alkyl or alkenyl chain or mixtures thereof
  • Z is derived from a reducing sugar such as glucose, fructose, maltose, lactose, in a reductive amination reaction.
  • compositions according to the present invention may further comprise a builder system.
  • a builder system Any conventional builder system is suitable, but preferred is a mixture of citric acid and a substituted succinic acid.
  • the citric acid builder employed in the practice of this invention will be present in the finished product in the form of any water-soluble salt of citric acid.
  • Such salts include, for example, sodium, potassium, ammonium or alkanolammonium salts.
  • a citric acid monohydrate slurry as a starting material, which will be neutralized in situ, so as to form the above mentioned salts.
  • the substituted succinic acid builders herein are of the general formula R-CH(COOH)CH2(COOH), i.e., derivatives of succinic acid, wherein R is C10-C16 alkyl or alkenyl, preferably C12-C14 alkenyl.
  • R is C10-C16 alkyl or alkenyl, preferably C12-C14 alkenyl.
  • These substituted succinic acid builders are preferably in the finished product in the form of their water-soluble salts, including the sodium, potassium, ammonium and alkanolammonium salts (e.g., mono-, di-, or tri-ethanolammonium).
  • As raw materials it is preferred to use these succinic acid derivatives in their diacid or anhydride form. The diacid will be neutralized in situ, while the anhydride will undergo a hydrolysis/neutralization process.
  • substituted succinic acid builders include : lauryl succinic acid, myristyl succinic acid, palmityl succinic acid, 2-dodecenyl succinic acid (preferred), 2-tetradecenyl succinic acid, and the like.
  • a preferred builder system comprises from 4% to 12% by weight of the total composition of the above substituted succinic acid builders, and from 4% to 12% by weight of the total composition of citric acid.
  • the compositions according to the invention may also contain a fatty acid. Preferred are oleic and palmitoleic acid.
  • compositions according to the invention preferably have a pH adjusted in the range of from 6 to 10, preferably from 7.5 to 8.0.
  • compositions according to the invention may also comprise an enzyme stabilizing system.
  • an enzyme stabilizing system provides a system wherein the protease does not significantly attack the native lipase, but the enzyme system or components thereof may still be subject to unstability problem due to the other detergency ingredients. Therefore, stabilizing agents may be needed, which are conventional and well known in the art.
  • a preferred enzyme stabilizing system is selected from boric acid, 1,2-propanediol, carboxylic acids, and mixtures thereof. These enzyme stabilizing systems are typically present in amounts of from 0.01% to 5% by weight of the total composition.
  • compositions of the invention may also comprise other enzymes.
  • enzymes such as cellulases or amylases.
  • Amylases particularly, seem to be stable in the presence of protease, and the compositions of the invention therefore preferably comprise an amylase.
  • compositions herein can contain a series of further optional ingredients.
  • the like additives include : suds regulants, opacifiers, agents to improve the machine compatibility in relation to enamel-coated surfaces, bactericides, dyes, perfumes, bleaches including perborate and percarbonate, brighteners, soil release agents, softening agents and the like.
  • the liquid compositions herein can contain further additives, typically at levels of from 0.05 to 5%.
  • additives include polyaminocarboxylates such as ethylenediaminotetracetic acid, diethylenetriaminopentacetic acid, ethylenediamino disuccinic acid or water-soluble alkali metals thereof.
  • Other additives include organo-phosphonic acids; particularly preferred are ethylenediamino tetramethylenephosphonic acid, hexamethylenediamino tetramethylenephosphonic acid, diethylenetriamino pentamethylenephosphonic acid and aminotrimethylenephosphonic acid.
  • compositions according to the invention are made by mixing the listed ingredients in the listed proportions.
  • compositions according to the invention are made by mixing the listed ingredients in the listed proportions 6 7 8 9 10 - Linear alkyl benzene sulfonate 5 7 9 8 10 - Sodium C12 ⁇ 15 alkyl sulfate 5 2 1.75 0 3 - C14 ⁇ 15 alkyl 2.5 times ethoxylated sulfate 2 0 2 0 0 - C12 glucose amide 6 0 7 0 0 - C12 ⁇ 15alcohol 7 times ethoxylated 0 0 0.5 0 11.6 - C12 ⁇ 15alcohol 5 times ethoxylated 0 8 0 8 - Oleic Acid 0 0 0 3.5 2.5 - Citric Acid 10 9 9.5 4 1 - C12 ⁇ 14 alkenyl substituted succinic acid 11 0 11.5 0 0 - STPP 0 20 0 0 0 - Zeolite 0 0 0 26 0 - Ethanol 6 4 4 3 6
  • compositions according to the invention are made by mixing the listed ingredients in the listed proportions 11 12 13 14 15 - Linear alkyl benzene sulfonate 5 7 9 8 10 - Sodium C12 ⁇ 15 alkyl sulfate 5 2 1.75 0 3 - C14 ⁇ 15 alkyl 2.5 times ethoxylated sulfate 2 0 2 0 0 - C12 glucose amide 6 0 7 0 0 - C12 ⁇ 15alcohol 7 times ethoxylated 0 0 0.5 0 11.6 - C12 ⁇ 15alcohol 5 times ethoxylated 0 8 0 8 - Oleic Acid 0 0 0 3.5 2.5 - Citric Acid 10 9 9.5 4 1 - C12 ⁇ 14 alkenyl substituted succinic acid 11 0 11.5 0 0 - Tartrate monosuccinate 0 15 0 17 20 - Diethoxylated poly (1,2 propylene terephtalate)

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  • Engineering & Computer Science (AREA)
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Abstract

Liquid detergent compositions are disclosed which contain conventional detergency ingredients and an enzyme system, wherein the enzyme system comprises a mixture of a lipase, or mixtures thereof, and a modified bacterial serine protease, or mixtures of said proteases..

Description

    Technical Field
  • The present invention relates to liquid detergent compositions which contain an enzyme system. The enzyme system is a combination of a modified protease and a lipase.
    • Background
  • It is well known in the art that detergent compositions may advantageously comprise enzyme systems. Such enzyme systems include cellulase, protease, lipase and amylases. The present invention is specifically aiming at providing liquid detergent compositions in which the enzyme system comprises a mixture of protease and lipase.
  • Formulating such a combination in a granular detergent raises no specific issue, since both enzymes can be physically separated. On the contrary, formulating such a combination in a liquid detergent raises a specific technical issue in that the protease is likely to take as a substrate any protein present in the detergent composition.
    Specifically, it has been observed that lipases which may also be present in the detergent composition are particularly subject to such proteolytic degradation; as a consequence, the residual activity of the lipase in the detergent composition will rapidly diminish with the storage time of the detergent composition, so that it was up to now impossible to formulate liquid detergent compositions comprising at the same time a lipase and a protease, said detergent compositions being sufficiently stable for a commercial exploitation.
  • It is thus an object of the present invention to provide a liquid detergent composition comprising an enzyme system comprising a lipase and a protease, wherein said enzyme system is stable; by stable, it is meant that the proteolytic degradation of the lipase is substantially reduced.
  • It has now been found that this object can be met by using any lipase, or mixtures thereof, together with a bacterial serine protease wherein the methionine adjacent to the serine of the active site has been replaced by another amino acid, or mixtures of such proteases. Indeed, it has been discovered that this specific combination would provide an enzyme system comprising a protease and a lipase, which would be stable in a liquid detergent composition.
  • This solution has the advantage of being simple because it only requires ingredients which are commercially available; indeed, several modified bacterial serine proteases suitable for the purpose of this invention are commercially available, as well as several lipases suitable for use in a detergent composition. Furthermore, the detergent compositions according to the invention require no addition of specific lipase stabilizers, and are therefore particularly attractive in terms of product cost and environmental compatibility.
  • Modified bacterial serine proteases including proteases suitable for use in the compositions according to the invention are disclosed for instance in EP-A-0 328 229 as well as their use in detergent compositions. This patent application describes among others a modified bacterial serine protease which is commercially available from GIST-BROCADES under the name MAXAPEM 15R
  • Biotechnology Newswatch, published March 1988, page 6, and EP-A-0 258 268 describe a lipase enzyme which is commercially available from NOVO NORDISK A/S under the trade name LIPOLASER. This European Patent application mentions that LIPOLASER can be combined with proteases to form a granular enzymatic detergent additive.
  • EP-A-0 381 262 describes detergent compositions comprising a protease and a lipase, preferably LIPOLASER, together with a stabilizing system. The proteases disclosed in this reference include bacterial proteases.
    • Summary of the invention
  • Accordingly, the present invention is a liquid detergent composition comprising an enzyme system, characterized in that the enzyme system comprises a modified bacterial serine protease or mixtures thereof, and a lipase or mixtures thereof. The bacterial serine protease is modified in that the methionine adjacent to the serine of the active site is substituted by another amino acid.
    • Detailed description of the invention
  • The enzyme system according to the present invention comprises a lipase and a protease. Any lipase suitable for use in a detergent composition can be used in the compositions according to the invention, as described for instance in EP 0 381 262 or EP 271 152. The preferred lipase to be used in the compositions according to the present invention is a lipase derived from Humicola lanuginosa, as described in EP-A-0 258 068 to NOVO INDUSTRI A/S. This patent application describes how to obtain said specific lipase, but said specific lipase is also commercially available from NOVO NORDISK A/S under the trade name LIPOLASER. Other commercially available lipases suitable for use herein are Amono-P Lipase R, Amono-B Lipase R, Amono CES Lipase R, Amono AKG Lipase R, all from Amono Pharmaceuticals, Japan; Toyo Jozo Co, Japan and US biochemical Corp. USA as well as Diosynth Co, NL also commercialize suitable lipases for use in the compositions according to the present invention.
  • The compositions according to the present invention typically comprise from 0.1 to 10000 Lipolytic Units per gram of finished product, preferably from 10 to 2500 Lipolytic Units per gram of finished product. Lipolytic units are defined for instance in EP 0 258 268, page 5 line 38..
  • The proteases to be used according to the present invention are modified bacterial serine proteases. All native bacterial serine proteases are characterized in that the active site invariably comprises a triade of amino acids which are serine, histidine and aspartic acid. These amino acids are positioned in the native form of the enzyme in such a way that they catalyse the cleavage of internal peptide bonds of proteins. Another common point between these bacterial serine proteases is that there always is a methionine adjacent to the serine of the active site, in the native sequence. The bacterial serine proteases suitable for use according to the present invention are those wherein the methionine adjacent to the serine of the active site has been substituted by another amino acid. The serine of the active site can also be defined as the serine which is homologuous to the serine in position 221 in the amino acid sequence of the bacterial subtilisin protease produced by Bacillus Subtilis; said sequence is listed herein after as SEQ ID NO 1
       In the sequence of this bacterial subtilisin protease produced by Bacillus Subtilis, the methionine is immediately after the serine in position 221 and therefore it is the methionine in position 222 which needs to be substituted by another amino acid. It is possible that, in the sequence of other bacterial serine proteases, this methionine would not be immediately following the serine of the active site; in such a case, it is the methionine homologuous to the methionine in position 222 in the sequence of this bacterial subtilisin protease produced by Bacillus Subtilis which needs to be substituted by another amino acid.
  • It is to be understood that the present invention does not reside in these modified proteases per se, rather in the particular application of these modified proteases to liquid detergent compositions also comprising a lipase; it is therefore not the aim of the present description to specify how these modified proteases can be obtained; This modification can be done by site-directed mutagenesis or any other genetic engeneering technique well known in the art for this purpose; for instance, EP-A-0 328 229, to GIST-BROCADES N.V. describes how to obtain such proteases. Another suitable method is described in EP 130 756, which also describes a mofified bacterial serine protease suitable for use in the compositions according to the invention.
       Furthermore, some modified bacterial serine proteases suitable for use in the compositions according to the invention are commercially available, such as DURAZYM R from NOVO, which is the methionine modified version of SAVINASE R; another example of available modified protease is MAXAPEM 15 from GIST-BROCADES, which is the modified version of MAXACALR wherein the methionine in position 216 has been substituted. Also available are experimental samples of modified OPTICLEAN R and OPTIMASER, from SOLVAY enzymes; both are modified in that the methionine in position 222 is substituted by a cysteine. Preferred modified bacterial serine protease according to the present invention are MAXAPEM 15R from GIST BROCADES and DURAZYM R from NOVO.
  • The compositions according to the present invention typically will contain from 0.005 to 10 mg of active protease per gram of finished product, preferably from 0.01 to 5.0 mg of active protease per gram of finished product. Mixtures of the modified bacterial serine protease described herein above are also suitable for use in the compositions according to the invention.
  • The rest of the liquid detergent composition according to the present invention is made of conventional detergency ingredients, i.e. water, surfactants, builders and others. The following description of these ingredients is for the sake of completeness of the description and is not to be construed as limiting the compositions of the present invention to those conventional ingredients described.
    The liquid detergent compositions herein comprises from 5% to 60% by weight of the total liquid detergent composition, preferably from 10% by weight to 40% by weight of an organic surface-active agent selected from nonionic, anionic, cationic and zwitterionic surface-active agents and mixtures thereof.
  • Suitable anionic surface-active salts are selected from the group of sulfonates and sulfates. The like anionic surfactants are well-known in the detergent arts and have found wide application in commercial detergents. Preferred anionic water-soluble sulfonate or sulfate salts have in their molecular structure an alkyl radical containing from about 8 to about 22 carbon atoms.
  • Examples of such preferred anionic surfactant salts are the reaction products obtained by sulfating C₈-C₁₈ fatty alcohols derived from e.g. tallow oil, palm oil, palm kernel oil and coconut oil; alkylbenzene sulfonates wherein the alkyl group contains from about 9 to about 15 carbon atoms; sodium alkylglyceryl ether sulfonates; ether sulfates of fatty alcohols derived from tallow and coconut oils; coconut fatty acid monoglyceride sulfates and sulfonates; and water-soluble salts of paraffin sulfonates having from about 8 to about 22 carbon atoms in the alkyl chain. Sulfonated olefin surfactants as more fully described in e.g. U.S. Patent Specification 3,332,880 can also be used. The neutralizing cation for the anionic synthetic sulfonates and/or sulfates is represented by conventional cations which are widely used in detergent technology such as sodium, potassium or alkanolammonium.
  • A suitable anionic synthetic surfactant component herein is represented by the water-soluble salts of an alkylbenzene sulfonic acid, preferably sodium alkylbenzene sulfonates, preferably sodium alkylbenzene sulfonates having from about 10 to 13 carbon atoms in the alkyl group.Another preferred anionic surfactant component herein is sodium alkyl sulfates having from about 10 to 15 carbon atoms in the alkyl group.
  • The nonionic surfactants suitable for use herein include those produced by condensing ethylene oxide with a hydrocarbon having a reactive hydrogen atom, e.g., a hydroxyl, carboxyl, or amido group, in the presence of an acidic or basic catalyst, and include compounds having the general formula RA(CH₂CH₂O)nH wherein R represents the hydrophobic moiety, A represents the group carrying the reactive hydrogen atom and n represents the average number of ethylene oxide moieties. R typically contains from about 8 to 22 carbon atoms They can also be formed by the condensation of propylene oxide with a lower molecular weight compound. n usually varies from about 2 to about 24.
  • A preferred class of nonionic ethoxylates is represented by the condensation product of a fatty alcohol having from 12 to 15 carbon atoms and from about 4 to 10 moles of ethylene oxide per mole or fatty - alcohol. Suitable species of this class of ethoxylates include : the condensation product of C₁₂-C₁₅ oxo-alcohols and 3 to 9 moles of ethylene oxide per mole of alcohol; the condensation product or narrow cut C₁₄-C₁₅ oxo-alcohols and 3 to 9 moles of ethylene oxide per mole of fatty(oxo)alcohol; the condensation product of a narrow cut C₁₂-C₁₃ fatty(oxo)alcohol and 6,5 moles of ethylene oxide per mole of fatty alcohol; and the condensation products of a C₁₀-C₁₄ coconut fatty alcohol with a degree of ethoxylation (moles EO/mole fatty alcohol) in the range from 4 to 8. The fatty oxo alcohols while mainly linear can have, depending upon the processing conditions and raw material olefins, a certain degree of branching, particularly short chain such as methyl branching. A degree of branching in the range from 15% to 50% (weight%) is frequently found in commercial oxo alcohols.
  • Suitable cationic surfactants include quaternary ammonium compounds of the formula R₁R₂R₃R₄N⁺ where R₁,R₂ and R₃ are methyl groups, and R₄ is a C₁₂₋₁₅ alkyl group, or where R₁ is an ethyl or hydroxy ethyl group, R₂ and R₃ are methyl groups and R₄ is a C₁₂₋₁₅ alkyl group.
  • Zwitterionic surfactants include derivatives of aliphatic quaternary ammonium, phosphonium, and sulfonium compounds in which the aliphatic moiety can be straight or branched chain and wherein one of the aliphatic substituents contains from about 8 to about 24 carbon atoms and another substituent contains, at least, an anionic water-solubilizing group. Particularly preferred zwitterionic materials are the ethoxylated ammonium sulfonates and sulfates disclosed in U.S. Patents 3,925,262, Laughlin et al., issued December 9, 1975 and 3,929,678, Laughlin et al., issued December 30, 1975.
  • Semi-polar nonionic surfactants include water-soluble amine oxides containing one alkyl or hydroxy alkyl moiety of from about 8 to about 28 carbon atoms and two moieties selected from the group consisting of alkyl groups and hydroxy alkyl groups, containing from 1 to about 3 carbon atoms which can optionally be joined into ring structures.
  • Also suitable are Poly hydroxy fatty acid amide surfactants of the formula R²-C-N-Z, wherein R¹ is H,



            O R¹



    C₁₋₄hydrocarbyl, 2-hydroxy ethyl, 2-hydroxy propyl or a mixture thereof, R₂ is C₅₋₃₁ hydrocarbyl, and Z is a polyhydroxyhydrocarbyl having a linear hydrocarbyl chain with at least 3 hydroxyls directly connected to the chain, or an alkoxylated derivative thereof. Preferably, R₁ is methyl, R₂ is a straight C₁₁₋₁₅ alkyl or alkenyl chain or mixtures thereof, and Z is derived from a reducing sugar such as glucose, fructose, maltose, lactose, in a reductive amination reaction.
  • The compositions according to the present invention may further comprise a builder system. Any conventional builder system is suitable, but preferred is a mixture of citric acid and a substituted succinic acid.
  • The citric acid builder employed in the practice of this invention will be present in the finished product in the form of any water-soluble salt of citric acid. Such salts include, for example, sodium, potassium, ammonium or alkanolammonium salts. In practice it is convenient to use a citric acid monohydrate slurry as a starting material, which will be neutralized in situ, so as to form the above mentioned salts.
  • The substituted succinic acid builders herein are of the general formula R-CH(COOH)CH₂(COOH), i.e., derivatives of succinic acid, wherein R is C₁₀-C₁₆ alkyl or alkenyl, preferably C₁₂-C₁₄ alkenyl.
       These substituted succinic acid builders are preferably in the finished product in the form of their water-soluble salts, including the sodium, potassium, ammonium and alkanolammonium salts (e.g., mono-, di-, or tri-ethanolammonium).
       As raw materials, it is preferred to use these succinic acid derivatives in their diacid or anhydride form. The diacid will be neutralized in situ, while the anhydride will undergo a hydrolysis/neutralization process.
       Specific examples of substituted succinic acid builders include : lauryl succinic acid, myristyl succinic acid, palmityl succinic acid, 2-dodecenyl succinic acid (preferred), 2-tetradecenyl succinic acid, and the like.
       A preferred builder system comprises from 4% to 12% by weight of the total composition of the above substituted succinic acid builders, and from 4% to 12% by weight of the total composition of citric acid. As an alternative builder, the compositions according to the invention may also contain a fatty acid. Preferred are oleic and palmitoleic acid.
  • It is well known from the man skilled in the art that the pH of the composition may significantly affect the enzyme system's performance. Accordingly, the compositions according to the invention preferably have a pH adjusted in the range of from 6 to 10, preferably from 7.5 to 8.0.
  • The compositions according to the invention may also comprise an enzyme stabilizing system. Indeed, the present invention provides a system wherein the protease does not significantly attack the native lipase, but the enzyme system or components thereof may still be subject to unstability problem due to the other detergency ingredients. Therefore, stabilizing agents may be needed, which are conventional and well known in the art. A preferred enzyme stabilizing system is selected from boric acid, 1,2-propanediol, carboxylic acids, and mixtures thereof. These enzyme stabilizing systems are typically present in amounts of from 0.01% to 5% by weight of the total composition.
  • The compositions of the invention may also comprise other enzymes. such as cellulases or amylases. Amylases, particularly, seem to be stable in the presence of protease, and the compositions of the invention therefore preferably comprise an amylase.
  • The compositions herein can contain a series of further optional ingredients. Examples of the like additives include : suds regulants, opacifiers, agents to improve the machine compatibility in relation to enamel-coated surfaces, bactericides, dyes, perfumes, bleaches including perborate and percarbonate, brighteners, soil release agents, softening agents and the like.
  • The liquid compositions herein can contain further additives, typically at levels of from 0.05 to 5%. These additives include polyaminocarboxylates such as ethylenediaminotetracetic acid, diethylenetriaminopentacetic acid, ethylenediamino disuccinic acid or water-soluble alkali metals thereof. Other additives include organo-phosphonic acids; particularly preferred are ethylenediamino tetramethylenephosphonic acid, hexamethylenediamino tetramethylenephosphonic acid, diethylenetriamino pentamethylenephosphonic acid and aminotrimethylenephosphonic acid.
    • EXAMPLES
  • The following compositions according to the invention are made by mixing the listed ingredients in the listed proportions.
    1 2 3 4 5
    - Linear alkyl benzene sulfonate 12 7 6 7 8
    - Sodium C₁₂₋₁₅ alkyl sulfate 2 2 3 3 2
    - C₁₄₋₁₅ alkyl 2.5 times ethoxylated sulfate 0 0 2 2 0
    - C₁₂ glucose amide 0 0 6 6 0
    - C₁₂₋₁₅alcohol 7 times ethoxylated 8 0 0 0 0
    - C₁₂₋₁₅alcohol 5 times ethoxylated 0 8 0 0 8
    - Oleic Acid 2 0 0 0 0
    - Citric Acid 3 9 9 13 15
    - C₁₂₋₁₄ alkenyl substituted succinic acid 10 5 5 7 6
    - Ethanol 4 4 3 4 5
    - 1,2-propanediol 2 3 3 1 2
    - NaOH 6 8 8 11 11
    - diethylene triamine penta(methylene phosphonic acid) 0.5 0.7 0.7 1 1
    - Amylase(143KNU/g) 0.1 0.1 0.05 0.2 0.1
    - LipolaseR(100KLU/g commercial solution) 0.4 0.2 0.3 0.3 0.3
    - PEM15R(50mg/g Commercial solution) 0.3 0 0 0 0.4
    - DurazymR (39 mg/g Commercial solution) 0 0.2 0 0 0
    - Opticlean M222CR (experimental sample) 0 0.1 0 0.4 0
    - Optimase M222CR (experimental sample) 0 0 0.3 0 0
    - CaCl2 0.01 0 0.01 0.01 0.02
    - Na metaborate 2.2 2 2 4 3
    - TEA 0 0 0 0 0
    - Sodium formate 0 0 0 0 0
    - Fatty Acids 0 0 0 0 0
    - Water and Minors Balance to 100%
    • EXAMPLES
  • The following compositions according to the invention are made by mixing the listed ingredients in the listed proportions
    6 7 8 9 10
    - Linear alkyl benzene sulfonate 5 7 9 8 10
    - Sodium C₁₂₋₁₅ alkyl sulfate 5 2 1.75 0 3
    - C₁₄₋₁₅ alkyl 2.5 times ethoxylated sulfate 2 0 2 0 0
    - C₁₂ glucose amide 6 0 7 0 0
    - C₁₂₋₁₅alcohol 7 times ethoxylated 0 0 0.5 0 11.6
    - C₁₂₋₁₅alcohol 5 times ethoxylated 0 8 0 8
    - Oleic Acid 0 0 0 3.5 2.5
    - Citric Acid 10 9 9.5 4 1
    - C₁₂₋₁₄ alkenyl substituted succinic acid 11 0 11.5 0 0
    - STPP 0 20 0 0 0
    - Zeolite 0 0 0 26 0
    - Ethanol 6 4 4 3 6
    - 1,2-propanediol 3 2 2 2 1.5
    - NaOH 9 9 9.8 9 3.5
    - diethylene triamine penta(methylene phosphonic acid) 1.0 1.0 1.0 0.5 0.8
    - Amylase(143KNU/g) 0.2 0.1 0.2 0.05 1
    - LipolaseR(100KLU/g commercial solution) 0.5 0.5 0.3 0.2 0.3
    - PEM15R(50mg/g Commercial solution) 0.4 0 0 0 0.2
    - DurazymR (39 mg/g Commercial solution) 0 0 0.5 0 0.2
    - Opticlean M222CR (experimental sample) 0 0 0 0.3 0
    - Optimase M222CR (experimental sample) 0 0.5 0 0 0
    - CaCl2 0.01 0.01 0.02 0.02 0.01
    - Na metaborate 4 2 4 3 0
    - TEA 0 0 0 0 6
    - Sodium formate 0 0 0 0 1
    - Fatty Acids 0 0 0 0 12
    - Water and Minors Balance to 100%
    • EXAMPLES
  • The following compositions according to the invention are made by mixing the listed ingredients in the listed proportions
    11 12 13 14 15
    - Linear alkyl benzene sulfonate 5 7 9 8 10
    - Sodium C₁₂₋₁₅ alkyl sulfate 5 2 1.75 0 3
    - C₁₄₋₁₅ alkyl 2.5 times ethoxylated sulfate 2 0 2 0 0
    - C₁₂ glucose amide 6 0 7 0 0
    - C₁₂₋₁₅alcohol 7 times ethoxylated 0 0 0.5 0 11.6
    - C₁₂₋₁₅alcohol 5 times ethoxylated 0 8 0 8
    - Oleic Acid 0 0 0 3.5 2.5
    - Citric Acid 10 9 9.5 4 1
    - C₁₂₋₁₄ alkenyl substituted succinic acid 11 0 11.5 0 0
    - Tartrate monosuccinate 0 15 0 17 20
    - Diethoxylated poly (1,2 propylene terephtalate) 1.0 0.5 0.7 0 0.5
    - Ethanol 6 4 4 3 6
    - 1,2-propanediol 3 2 2 2 1.5
    - NaOH 9 9 9.8 9 3.5
    - diethylene triamine penta(methylene phosphonic acid) 1.0 1.0 1.0 0.5 0.8
    - Amylase(143KNU/g) 0.2 0.1 0.2 0.05 1
    - LipolaseR(100KLU/g commercial solution) 0.5 0.5 0.3 0.2 0.3
    - PEM15R(50mg/g Commercial solution) 0.4 0 0 0 0.2
    - DurazymR (39 mg/g Commercial solution) 0 0 0.5 0 0.2
    - Opticlean M222CR (experimental sample) 0 0 0 0.3 0
    - Optimase M222CR (experimental sample) 0 0.5 0 0 0
    - CaCl2 0.01 0.01 0.02 0.02 0.01
    - Na metaborate 4 2 4 3 0
    - TEA 0 0 0 0 6
    - Sodium formate 0 0 0 0 1
    - Fatty Acids 0 0 0 0 12
    - Water and Minors Balance to 100%
    Figure imgb0001
    Figure imgb0002

Claims (11)

  1. A liquid detergent composition comprising conventional detergent ingredients and an enzyme system comprising a lipase or mixtures thereof and a protease, wherein the protease is a bacterial serine protease wherein the methionine adjacent to the serine of the active site has been replaced by another amino acid, or mixtures of said proteases.
  2. A liquid detergent composition according to claim 1 wherein the lipase is LIPOLASE R.
  3. A detergent composition according to claims 1 or 2 which comprises a lipase in amounts so as to obtain from 0.1 to 10000 Lipolytic Units per gram of finished product.
  4. A detergent composition according to any of the preceeding claims which comprises a protease according to claim 1 or mixtures thereof, in amounts such as to obtain from 0.005 to 10 mg of active protease per gram of finished product.
  5. A detergent composition according to any of the preceeding claims wherein the modified bacterial serine protease is selected from the group of MAXAPEM 15 R, DURAZYM R, M222C OPTICLEAN R and M222C OPTIMASE R, or mixtures thereof.
  6. A detergent composition according to any of the preceding claims which additionally comprises an amylase.
  7. A detergent composition according to any of the preceding claims which additionally comprises an enzyme stabilization system.
  8. A detergent composition according to claim 7 wherein the enzyme stabilization system consists of from 0.01% to 5% by weight of the total composition of boric acid.
  9. A detergent composition according to claims 7 or 8 wherein the enzyme stabilization system comprises 1,2-propane diol and/or carboxylic acids and mixtures thereof.
  10. A detergent composition according to any of the preceding claims which has a pH in the range of from 6 to 10.
  11. A detergent composition according to claim 9, which has a pH of from 7.0 to 8.5.
EP19910200149 1990-11-14 1991-01-25 Liquid detergent composition containing lipase and protease Expired - Lifetime EP0486073B1 (en)

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DE1991621059 DE69121059T2 (en) 1990-11-14 1991-01-25 Liquid detergent composition containing lipase and protease
ES91200149T ES2091280T3 (en) 1990-11-14 1991-01-25 LIQUID DETERGENT COMPOSITION CONTAINING LIPASE AND PROTEASE.
EP19910200149 EP0486073B1 (en) 1990-11-14 1991-01-25 Liquid detergent composition containing lipase and protease
DK91200149T DK0486073T3 (en) 1990-11-14 1991-01-25 Liquid detergent composition containing lipase and protease
JP4500878A JPH06504555A (en) 1990-11-14 1991-11-04 Liquid detergent composition containing lipase and protease
AU90416/91A AU9041691A (en) 1990-11-14 1991-11-04 Liquid detergent composition containing lipase and protease
CA002096256A CA2096256C (en) 1990-11-14 1991-11-04 Liquid detergent composition containing lipase and protease
PCT/US1991/008041 WO1992008779A1 (en) 1990-11-14 1991-11-04 Liquid detergent composition containing lipase and protease
MX9102022A MX9102022A (en) 1990-11-14 1991-11-12 LIQUID DETERGENT COMPOSITION CONTAINING LIPASE AND PROTEASE
NZ24057191A NZ240571A (en) 1990-11-14 1991-11-13 Liquid detergents containing an enzyme system of lipase and modified protease
IE395191A IE913951A1 (en) 1990-11-14 1991-11-13 Liquid detergent composition containing lipase and protease
CN 91111550 CN1062374A (en) 1990-11-14 1991-11-14 The liquid detergent composition that contains lipase and proteolytic enzyme
TR106991A TR27423A (en) 1990-11-14 1991-12-04 Liquid detergent containing lipase and protease.
TW81100525A TW215108B (en) 1990-11-14 1992-01-25
US08/322,965 US5733473A (en) 1990-11-14 1994-10-13 Liquid detergent composition containing lipase and protease

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WO1993008253A1 (en) * 1991-10-16 1993-04-29 Unilever N.V. Aqueous enzymatic detergent compositions
WO1995017494A1 (en) * 1993-12-23 1995-06-29 Henkel Kommanditgesellschaft Auf Aktien Enzyme-containing liquid washing agent
US5442100A (en) * 1992-08-14 1995-08-15 The Procter & Gamble Company β-aminoalkyl and β-N-peptidylaminoalkyl boronic acids
US5472628A (en) * 1991-04-30 1995-12-05 The Procter & Gamble Company Liquid detergents with an aryl acid for inhibition of proteolytic enzyme
EP2199386A1 (en) 1993-10-08 2010-06-23 Novozymes A/S Amylase variants
EP3074494B1 (en) 2013-11-27 2018-04-04 Henkel AG & Co. KGaA Lipase stabilization in dishwashing detergents
EP2250246B1 (en) * 2008-01-28 2019-10-02 Ecolab USA Inc. Multiple enzyme cleaner for surgical instruments and endoscopes

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JP2859520B2 (en) * 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ Lipase, microorganism producing the same, method for producing lipase, and detergent composition containing lipase
JP4733861B2 (en) * 2001-06-20 2011-07-27 花王株式会社 Enzyme particles
JP2013515139A (en) * 2009-12-21 2013-05-02 ダニスコ・ユーエス・インク Detergent composition containing lipase from Thermobifida fusca and method of use
CN102444018B (en) * 2011-10-26 2013-07-31 芜湖南翔羽绒有限公司 Biochemical combination precision washing technology for eiderdown
CN102604754B (en) * 2012-02-03 2013-07-03 深圳市绿微康生物工程有限公司 Biological enzyme cleaning agent and method for preparing same
WO2016206837A1 (en) * 2015-06-26 2016-12-29 Unilever Plc Laundry detergent composition
CN107922897A (en) * 2015-08-28 2018-04-17 荷兰联合利华有限公司 The liquid detergent compositions of enzyme comprising protease and non-protein enzyme

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US5472628A (en) * 1991-04-30 1995-12-05 The Procter & Gamble Company Liquid detergents with an aryl acid for inhibition of proteolytic enzyme
WO1993008253A1 (en) * 1991-10-16 1993-04-29 Unilever N.V. Aqueous enzymatic detergent compositions
US5442100A (en) * 1992-08-14 1995-08-15 The Procter & Gamble Company β-aminoalkyl and β-N-peptidylaminoalkyl boronic acids
EP2199386A1 (en) 1993-10-08 2010-06-23 Novozymes A/S Amylase variants
WO1995017494A1 (en) * 1993-12-23 1995-06-29 Henkel Kommanditgesellschaft Auf Aktien Enzyme-containing liquid washing agent
EP2250246B1 (en) * 2008-01-28 2019-10-02 Ecolab USA Inc. Multiple enzyme cleaner for surgical instruments and endoscopes
EP3074494B1 (en) 2013-11-27 2018-04-04 Henkel AG & Co. KGaA Lipase stabilization in dishwashing detergents

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WO1992008779A1 (en) 1992-05-29
CN1062374A (en) 1992-07-01

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