五、發明説明（l )5. Description of the invention (l)
0 A 6 Β6 本發明係闞於含有酵素的液態清潔姐合物。該酵素糸统 是结合改良的脂肪梅及蛋白酶。 發明背暑 眾所週知的技蘐中，清潔劑姐合物可方便地含有酵素系 统這些酵素糸统包括纖維素酶、蛋白B及豭粉®。本發明 尤其指望提供在液態清潔劑姐合物中的酵素系統包含蛋白 酶及脂肪酶。 對以這種结合形式的粒狀清潔劑沒有提出任何特殊發佈 兩種酵素可以物理方式分離。反之*對Μ這棰结 合形式的潔劑提出了特殊的技術發佈•其中蛋白酶 似乎可以當成是任何存在於清潔劑姐合物中蛋白質的底物 (請先閲讀背面之注意事項再填寫本頁) 經濟部屮央標準沿3工消费合作社印製 特定 肪梅特 劑姐合 期快速 合物同 夠的阐 1人1此 _及蛋 统是S 用減少 目前 言之，頃覲察到也可以存在於清潔劑姐合物中的脂 別會受到這棰蛋白解的降解作用；结果是在清潔 物中脂肪酶的殘餘活性將會使清潔劑姐合物的儲存 地縮減，故至目前為止不可能形成的液態清潔劑姐 時含有脂肪酶及蛋白酶，上述的清潔劑姐合物有足 定性供商業利用。 本發明的丨Ξ]地记促供一 Μ & W的祁尜糸统U山脂肪 白胸所姐成的液Μ濟潔劑姐合物•其中上述酵索系 定的：所謂S定S指會確宵地使蛋白水解的降解作 已發現可利用任何脂肪酶或彼等的泪合物與细Μ絲 本紙張尺度逍用中國國家標準(CHS)甲4規格（210x297公货) 五、發明説明（2 ) _或這些蛋白梅的混合物一起達到本目地，其中 與絲氧酸活性位置鄰接的蛋氨酸已被另一個胺基酸取代。 事實上’已發現埴種持殊的结合可提供一種含有脂肪醻及 蛋白酶的酵素系统會在液態清潔劑姐合物中具有穩定性。 本溶液具有的優點是單純，因為它只需要具有商樂價值 一丨— I—. ....________________ 的成份即可；事實上，有數棰具商業價值的改良的细菌絲 氨_蛋白酶合於本發明的目地，及有數種脂肪梅適合在清 潔劑姐合物中使用。而且根據本發明的清潔劑姐合物不需 要加入特別的脂肪豳安定劑，而因此在產品價格及環境迪 用性方面具有特殊的吸引力。 在舉例的歐洲專利申請案第0 328 229號中掲示適合於 在根據本發明的姐合物中使用包括蛋白酶的改良细菌絲氨 酸蛋白酶及彼等在清潔劑姐合物中的用途。本專利申請荼 說明其中一個改良细菌絲氨酸蛋白酶，其商業價值來自於 以 M A X A P E Μ 為名稱的 G I S T - B R 0 C A D E S 。 (請先閲讀背面之注意事項再填寫本頁) 裝- 線- 於 表 發 的 月 三 年 6 第 中 第 案 請 Φ 利 專 歐 及 頁 素 酵 酶 肪 脂 種 1 明 說 號 經濟部屮央楳準局Α工消#合作社印製 '—- HJ P1 々π, τι L φ Μ 利 於爯 自洲 來歐 值本 "aο 的 S 上A/ 商 其0 A 6 Β6 The present invention is a liquid cleansing compound containing enzymes. The enzyme system is combined with improved fat plum and protease. Invention of the summer is a well-known technique, the detergent compound can conveniently contain enzyme systems. These enzyme systems include cellulase, protein B and fermented powder®. In particular, the present invention is expected to provide an enzyme system in a liquid detergent composition including protease and lipase. No particular release is proposed for the granular detergent in this combined form. The two enzymes can be physically separated. On the contrary * Special technical releases have been proposed for this combination of detergents. Among them, protease seems to be used as a substrate for any protein present in the detergent compound (please read the precautions on the back before filling this page) The Ministry of Economic Affairs ’standard is printed along with the 3 industrial and consumer cooperatives to print a specific compound of the specific fat medicinal agent. The combination is sufficient to explain 1 person 1 this _ and egg system is S used to reduce the current word, it can be observed that it can exist The fat in the detergent compound will be degraded by this protein hydrolysis; the result is that the residual activity of lipase in the cleaning agent will reduce the storage place of the detergent compound, so it has not been possible so far The formed liquid detergent contains lipase and protease, and the above detergent composition is fully qualitative for commercial use. The present invention is a liquid liquid detergent composition composed of Qi Mitsubishi U mountain fat white chest made by one M & W. The above enzymes are fixed: the so-called S fixed S Refers to the degradation of proteolysis that can be used in the night. It has been found that any lipase or their lacrimal compounds and fine papers can be used in accordance with the Chinese National Standard (CHS) A4 specifications (210x297 public goods). V. Description of the invention (2) _ or a mixture of these protein plums achieves this object together, in which the methionine adjacent to the active position of seric acid has been replaced by another amino acid. In fact, it has been found that the unique combination of species can provide an enzyme system containing fat mash and protease that will be stable in the liquid detergent composition. The advantage of this solution is simplicity, because it only needs the ingredients with commercial value-I-..... ________________; in fact, there are several improved bacterial serine proteases with commercial value. For the purpose of the present invention, there are several types of fatty plums suitable for use in detergent compositions. Moreover, the detergent composition according to the present invention does not require the addition of a special fat stabilizer, and therefore has a special attraction in terms of product price and environmental applicability. The example European Patent Application No. 0 328 229 shows the use of modified bacterial serine proteases including proteases in the sister compound according to the present invention and their use in detergent compounds. This patent application shows that one of the improved bacterial serine proteases has commercial value derived from GIST-BROCCADES under the name MAXAPEE. (Please read the precautions on the back before filling out this page) Install-Line-Issued on the table for the third year of the month 6 The first case please Φ Lithuania and Peptidase Fatty Acid Species 1 Clearly stated Ministry of Economic Affairs Yang Yang Quasi-bureau Α 工 消 # Cooperative Society Printed --- HJ P1 々π, τι L φ Μ is good for S from AZhou to the European value book " aο A / Shangqi
K S 白 νοίΗ ο ! N a. 的可 s. E 稱 S 名!.A 業PO 商1.1 為及 E !. S1" 包 物 合 姐 劑 濟 。 的 物明 加說 添號 劑 2 潔26 淸1 索38 酵 ο 的第 狀案 粒13 成申 形利 W 0. 合洲 結歐 0 在 统 糸 定 安 個 1 、 和酶 梅白 肪蛋 S 菌 的细 佳括 為包 SE酶 LA白 PO蛋 L1的 Μ 示 及揭 酶中 白考 蛋參 含本 逍 度 張 紙 本 準 標 家 國 _ 215108 Λ 6 Β6 五、發明説明（3) 經濟部屮央櫺準A工消费合作社印製 太發明的說明 因此，本發明是含有酵素糸统的液態清潔劑姐合物，其 持徴是酵素系統包含改良妞菌絲氨酸蛋白酶或彼等的混合 物及脂肪酶混合物或彼等的混合物。將另一個胺基酸取代 與细菌絲氨活性位置鄰接的蛋氨酸Μ改良细菌絲氨酸蛋白 酶。本發明的詳Μ說明根據本發明的酵素系统包含脂肪酶及蛋白酶。Κ舉例的 歐洲專利申請案第0 381 262號及第271 152號的說明為根據，任何適合於在清潔劑姐合物中使用的脂肪酶都可以 用於根據本發明的姐合物中。被用於Μ本發明為根據的姐 合物中較佳的脂肪海是衍生自Huraico la lanuginosa ，如 在歐洲專利申請荼第0 258 068號說明的NOVO I N D I! S T R I A / S。本專利申請案說明如何獲得上述特殊的脂 肪_ •但上述特殊的脂肪酶在商業上的價值來自於以 L I P 0 L A S E為商業名稱的N 0 V 0 N 0 R D I S K A / S «其它適合於在 此使闬貝商樂價值之脂肪_是A η ο η 〇 - P脂肪、A H ο η 〇 - B脂 肪、A m ο η ο (' Ε S Ul1 肪、A ra () η ο Λ Κ G 脂肪、PJf 丫._1 來 Γ.Ι [:i 木的 T 0 y 0 J ο z 〇公司之A m ο η 0製藥及美國的1丨S生丨l：公司與N L的 D i o s y n t h公司也Μ商業化適當的脂肪酶供在根邯本發明的 姐合物中使用。 根拇本發明的姐合物一般包含每克Μ·约淹品從〇 . 1至 (請先閱讀背面之注意事項再塡寫本頁) 裝- 訂 線· 本紙尺度边用中國國家櫺準（CNS)甲4規格（210x297公:¢) 315108 Λ 6 Β6 經濟部屮央梂準局A工消费合作社印製 五、發明説明（4 ) 10000解脂單位· W每克最終產品從10至2500解脂單位較 佳，解脂單位的定義在舉例的歐洲專利申謫案第0 258 068號第5頁的第38行中。 根據本發明所使用的蛋白酶是改良细菌絲氨酸蛋白酶。 所有天然的细菌絲氨酸蛋白酶特徵是活性位置必然包含一 姐三個為絲氨酸的胺基酸、姐氨酸及天冬氨酸。這些胺基 酸位於酵素的天然形式中具催化蛋白質内部肽鍵裂開的這 種作用。在這些天然系列中的细菌絲氨酸蛋白酶之間的另 —個共同點是總是有一個蛋氨酸郯接在絲氨酸的活性位置 上。以本發明為根據，適於使用的细菌絲氨酸蛋白酶是那 些其中邮接在絲氨酸活性位置上的蛋氨酸已被另一個胺基 酸所取代的蛋白酶。也可K將活性位置絲氨酸定義成與位 於由枯草桿菌所產生的细菌枯草桿菌蛋白_的胺基酸糸 列中第2 2 1位置上的絲氨酸同性質的絲氨酸；上述系列 列於本文之後的圖1中。 在由枯草桿菌所產生的這個细菌枯草桿Μ蛋白酶的胺基 酸系列中，蛋氨酸直接地在2 2 1位置上的絲氨酸之後，因 此蛋氨酸是在222的位置上，蛋氨酸必須被另一個胺基酸 所取代，在其它的细菌絲氛酸蛋白_系列中，該蛋#ί时可 能不β ίί接地接注絲鉍酸的活性位找之後；作辺梯的坫肜 中，蛋镇與位於由坫革捍阑所逄生的细閉坫草榫Μ蛋白 fig的胺基酸系列中第2 2 2位ΙΪ上的蛋氨_性苕相同，蛋® 肋必泊被另一画胺基酸所収代… 已知本發明與其沒有這些改良的蛋白飽本身，不如將這 (請先閱讀背面之注意事項再填寫本頁) 裝· 訂 線. 本紙張尺度逍用中國國家標準（CNS)甲4規格（210x297公.11) 215108 Λ 6 Β 6 經濟部屮央櫺準沿订工消t合作社印製 五、發明説明（5) 些改良的蛋白梅之特殊應用至也含有脂肪酶的清 物中；因此對怎麽樣可以獲得這些改良的蛋白酴不 明詳细說明目搮；可向誘爱作用的位置或為達該目 本技藝中所热知的任何其它基因工程技術進行這個&$作 用；例如在歐洲專利申請案第0 328 229號中說明如肖獲 得這些蛋白酶GIST-BROCADES Ν.Ζ·。在歐洲專利申謫案第 0 32 8 229號中說明另外一個方法，也說明一涸適合於在 根據發明的组合物中使用的改良的细菌絲氨酸蛋白_。 而且適合於在根據發明的姐合物中使用的部份改良细菌 絲氨酸蛋白梅具有商業價值•如來自N0V0的DURAZYH .其 為蛋氨酸改良的SAVIN AS E改造物；另一涸有用的改良蛋白 酶之亩拖例是來自GI5T-BR0CADES的MAXAPEM 15，其為改 良的MAXACAL改造物，其中在第216位置上的蛋氨酸已被 取代。來自S0LVAY酵素的改良0PTICLEAN及0PTIMASE也是 有效的實驗實施例：以半胱氨酸取代在216位置上的蛋氨 酸使兩者改良。根據本發明較佳的改良细菌絲氨酸蛋白酶 是來自 GIST-BROCADES 的MAXAPEM 15及來自 N0V0的 D U R ή Z Y Μ 根拽本發明的姐合物一般包栝每克敁终痄品從0 . 〇 〇 5至 1 0带克的活性街白酶，以埒克Μ终齑品從0 . 0 1朵5 . 〇的活 性蛋白酶較佳，在上述的本文中改良的细Μ絲劄SS蛋白酶 的混合物也適合用於根據本發明的姐合物中。 以本發明為根據的液態淖潔劑姐合物的其餘部份是由熟 知的濟潔劑成份所姐成的，即水、表面活性劏 '肋i劑ft ^·—· —— — - 7 - 本紙5艮尺度逍用中S國家樣準（CNS)肀4規格（210x297公货） (請先閲讀背面之注意事項再填窝本頁) 裝· 訂- 線· 215108 A 6 B 6 五、發明説明（6) 其它成份等。Μ下對這些成份的說明是為了使說明完整性 ，而不是解釋將根據本發明的姐合物限定成所說明的那些 熟知的成份。 在本文中的液態清潔劑姐合物包含以總液態清潔劑姐合 物的重量計從5%至6%選自於非離子性、陰離子性、陽 離子性及兩性離子的表面活性劑與彼等的混合物之有櫬表 面活性劑以重量計從10%至40%較佳。 適當的陰離子表面活性劑選自於磺酸鹽及硫酸翹族群。 在清潔劑技藝中所熟知適合的陰離子表面活性劑已發現廣 泛應用於商業清潔劑中。在較佳的陰離子水溶性磺酸鹽或 硫酸鹽之分子结構中具有的烷基含有約8至約22個碳原子 Ο 經濟部屮央榫準劝员工消费合作社印製 (請先閲讀背面之注意事項再填寫本頁) 這些較佳的陰離子表面活性劑鹽類的實胞例是使衍生於 舉例的脂油、棕欄油、棕櫊仁油及椰子油等C β - C ^ 8脂肪醇 類；其中的烷基含有從約9至約15個碳原子的烷基苯磺酸 詡；烷基甘油醚磺酸钠；衍生於脂油及椰子油之脂肪醇的 _硫酸鹽及在烷基鐽中具有從約8至約22個碳原子之鏈烷 烴磺酸鹽的水溶性鹽類等硫酸化所獲得的反應產物。也可 Μ使用在舉例的美國專利說明書第3 . 3 3 2 , 8 8 0號中更完赘 説叫的硫酸化烯烴表Ιίι丨活性劑‘、由熟知的1¾離子使屮和的 賜離子代表险離子合成磺酸鹽及/或硫时鹽。該陽離子被 厣泛地使用於清潔劑技術中，如钠、鉀及烷醉銨等。 以烷基笨磺酸的水溶性鹽類代表本文中適當的陰離子合 成的表面活性劑組成，Μ烷基笨碲酿钠較佳，以烷基·中具 本紙張尺度边用中a國家標準（CNS)甲4規格（210X297公垃） 21510S Λβ ______B_^__ 五、發明説明（7 ) 有約10至.13涸碳原子的烷基苯磺酸納較佳。在本文中另一 個較佳的陰離子表面活性劑姐成是在烷基中具有約10至15 個碳原子的烷基疏酸納。 適合於在本文中使用的非離子性表面活性劑包括那些在 酸性或驗性觸媒的存在下使次乙基氧化物與具有反應性的 氫原子，即羥基羧基或胺基之類的碳氫化合物濃缩所產生 的試劑，並包括具有一般式為RA(CH2CH20)„H之化合物， 其中R代表憎水性部份，A代表帶有反應性氫原子的族群 及η代表次乙基氣化物部份的平均值。R通常包括從約8 至22個碳原子。也可Μ次丙基氧化物與分子量較低的化合 物之濃縮作用形成化合物。η通常從約2赛化至約24。 經濟部屮央標準工消费合作社印製 (請先閱讀背面之注意事項再填寫本頁) Κ具有從12至15個碳原子的脂肪醇與每莫耳或每一個脂 肪酵具有從約4至1 0莫耳的次乙基氣化物之濃縮產物代表 較佳的非離子性乙氧醇鹽類。該適當的乙氧酵馥類物筲包 括Ci2-Cls氧基一醇類與每莫耳醇有3至9莫耳的次乙基 氧化物之濃縮產物；C14-C15氧基一酵類窄豳份與每箅耳 脂肪（氧基）醇有3至9 莫耳的次乙基氧化物之濃縮產 物；Cl2-Cl3氧基一醇類智趟份脂肪（氧基）與每莫耳 脂肪酵有· 5辑耳的次乙基氧化物之溏縮產物及Ci 〇 - C i 4 椰子脂肪龄與乙铽酌化作以等級範幽從4 Φ: b (次.乙钻 氧化物的莫耳數/ 一莫耳的脂肪酸）之漶縮奄物。以製程 條件及烯烴原料為根撺，在大部份為線性的脂防氣基醉可 能具有待別短的鏈，如為支鏈的甲基。常發拟在商業中的 氣堪漭之支鏈程度在從丨5 %至5 0 % ( K喷坫計）的範園内 本紙5fc尺度边用中國國家標準（CNS)甲4規格（210x297公龙） Λ (ί η 6 五、發明説明（8) 通常的陽離子表面活性劑包括化學式為hRzHiT之四 级铵化合物，在此Ra、R2ftR3是甲基，而是（：12-(：15的 综基，或者在此Ri是乙基或經基乙基· R2及R3是甲基，而 1?4是Cl2-Cl5的院基。 兩性離子表面活性劑包括脂肪条四级銨、璘及化物等 的衍生物，脂肪系部份可Μ是直鏈或支鏈；而其中一個脂 肪系取代物包括從約8至約24個碳原子及另一饀取代物包 括至少一個以水溶解的陰離子族群。等別佳的兩性離子物 質是由拉裼林（Laughlin在1975年12月9日發表的美國專 利申謫案第3,925.262號及由拉福林et al .在丨975年 12月30日發表的美國專利申請案第3,929.678號所掲示的 乙氧醇化的磺酸銨及硫酸銨。 半極性的非離子表面活性劑包括的水溶性胺氧化物含有 一假從約8至約28画碳原子的烷基或羥基烷基部份及兩部 份選自由含有從1至約3個碳原子的烷基及羥基烷基所姐 成的族群，可視需要選擇使這些族群併入環结搆中。 化學式為卜^-叾的聚羥基脂肪酸醯胺表面活性劑也適 0 R 1 濟 ·:!Γ_ 屮 火 Ιΐ: 肀 Λ ί\ i- w1] fi， if W H中K 1足1丨、（：烴沾、2杈坫乙丛、2 抒梢闪 基或彼#的混合物，R 2是C β _ Ί i烴蜗，而ζ是具有在爲線 烴堪鐽有至少3個羥基直接速结在辨h的聚稃搞坪稱或彼 存保氣笺化衍生物，較佳地以R i是帀基，r ;ί是直键 梭基或烯蘂或彼等的港合物，而ζ镫衍生自在堪原 印 ------—----_10 - 本趴认尺度边用中S ®家4JiMCNS) ΤΜ規帒（2丨0x297公及） Λ 6 Β6 215108 五、發明説明（9) (請先閱讀背面之注意事項再填寫本頁) 胺化反.應中的堪原糖，如葡萄糖、果糖、麥芽糖、乳糖等 〇 以本發明為根據的组合物通包含一個肋洗劑系统。任何 热知的助洗劑糸统均適合，但較佳地是檸檬酸與取代的 丁二酸之混合物。 在本發明實例中使用的檸樺酸肋洗劑將以任何檸樺酸水 溶性鹽的形式存在於最終產品中。例如，造些鹽類包括納 、鉀、銨或烷酵銨鹽類。事實上可方便地使用將會在原位 置中和的棒樣酸單水合物泥漿為起始物Μ便於形成上述蘧 類。 在本文中取代的丁二酸助洗劑具有的一般式是 r-ch(cooh)ch2(cooh)，即丁二酸的衍生物，其中R是 c1〇-cie烷基或烯基，以〔:12-(：14烯基較佳。這些在最终產 品中的取代的丁二酸肋洗劑較佳地是K具有彼等的水溶性 鹽的形式，包括納、鉀、銨或烷酵銨鹽類（即單、二或三 —乙醇銨）。 認為使用這些在彼等的二酸或酐形式中的丁二酸衍生物 較佳。二酸將會在原位置上中和，而酐將會進行水解/中 和作用的製程。 ^濟部中央杧準：：^:::工消^合作社印^ W殊的収代的T 股肋洗宵例Q Μ I. 烷鹄丁二故、 十四烷稱丁二酸、十六烷基丁二酸，2 —十二烯S 丁二_ (較佳的）、2 —十四烯基丁二酸及類似物 較佳的肋洗劑系统包含Κ總姐合物的响i計從4 %至 1 2 %的上述取代的丁二醃肋洗劑及K缌姐合物的爾》計從 - 1 1 - 各认張尺度迈用t 3 a家诈準（CNS)肀4規格（210x297公兮） Λ 6 B 6 五、發明説明（i〇) 'll 4 %至 括脂肪 佳。 從本 的性能 物具有 至8 . 0 Μ本 。事菁 SS天然 它的清 熟知且 、1 ， .1 2 %的擰 酸為視需 技蕤热知 會有重大 的酸驗值 較佳。 發明為根 上，在本 的脂肪酶 潔劑成份 眾所週知 2 —丙二 系统一般地存在 樺酸。以本發明為根據的姐合物也可以包 要而選用的助洗劑。Μ油酸及棕櫊油酸較 的技巧得知姐合物的酸鹸值可對酵素糸统 地影W。因此，將以本發明為根據的姐合 調整在從6至10的範圍内較佳，以從7.5 據的姐合物也可以包含一個酵素安定糸统 發明提供的糸統中之蛋白酶不會顯著地攻 ..... . . 但該酵素糸统或組成仍可能受到由於其 的不安定問題。因此可能需要在本技藝中 的安定劑。較佳的酵素安定糸統選自硼酸 醇、羧酸及彼等的混合物。這些酵素安定 量是以總姐合物的重最計從0 . 0 1 %至5 % (請先閱讀背面之注意事項再填寫本頁) 本發明的姐合物也可以包含其它的酵素，如纖維素酶及 猥粉酶。在蛋白酶存在下的澱粉酶似乎是持別锞定_，因此 本發明的姐合物Μ含有澱粉酶較佳。 -ν\ 钗濟$-央標準Χ,ΙΗ工消1'合作社印虹 木文的m合物可μ包括一系统更可視需要而選用的成份 適出的添加物宵例包坫肥@水rnsu 1 an l.s 、遮光劑、 改進關於塗以琺瑯的表面之機械適合性的試劑、殺细菌劑 、染枓、番料、含有過硼酸醴及過碳酸蒲的漂白劑增亮劑 、h埚釋出劑、炊化劑及類似物。 木文中的液態姐合物可Μ包枯更岑的添加劑，一般是在 12 本紙張尺度边用中S國家.fi準（CNS) Τ 4規格（210 X 297公Ji) Λ 6 B 6 五、發明説明（ll ) 從0.0 5至5%的量。這些添加劑包括聚胺基羧酸鹽類，如 次乙基二胺基四_酸、二次乙基三胺基五»酸、次乙基二 胺基二丁二酸或彼等的水溶性驗金靨。其它的添加劑包括 有機膦酸；特別佳的是次乙基二胺基四次甲基膦酸、六次 甲基二胺基四次甲基膦酸、二次乙基三胺基五次甲基膦酸 及胺基三次甲基膦酸。 將所列的成份依陳列的比例琨合以製備根據本發明的下 列姐合物。 (請先閱讀背面之注意事項再填寫本頁) 濟部十央耵準劝U工消"合作社, 本认张尺度边用中S 3家樣準（CNS)甲.1規格（210x297公及） 51 2l5i〇8 Λ 6 B 6K S white νοίΗ ο! N a. The s. E is called the S name !. A business PO business 1.1 is and E !. S1 " Baowuhejiejijiji. Wu Mingjia said Tim No. 2 Jie 26 淸 1 Suo 38 fermentation ο the first case 13 Cheng Shen Xingli W 0. Hezhou Jieou 0 in Tongshi Ding'an 1, and the enzyme plum white fat egg S bacteria The detailed descriptions include the M display of the SE enzyme LA white PO egg L1, and the white test egg ginseng contained in this enzyme in the enzyme. The standard copy of the paper is _ 215108 Λ 6 Β6 V. Description of the invention (3) The description of the invention is printed by the Beijun A Industrial Consumer Cooperative Society. Therefore, the present invention is a liquid detergent composition containing an enzyme system. Or their mixture. Substituting another amino acid for methionine M adjacent to the active site of bacterial serine improves the bacterial serine protease. Detailed description of the invention The enzyme system according to the invention comprises lipase and protease. The descriptions of European Patent Application Nos. 0 381 262 and 271 152 exemplified by K are based on the description that any lipase suitable for use in the detergent composition can be used in the composition according to the present invention. The preferred fatty sea used in the sister compound based on the present invention is derived from Huraico la lanuginosa, as described in European Patent Application No. 0 258 068 NOVO I N D I! S T R I A / S. This patent application describes how to obtain the above-mentioned special fats _ • However, the commercial value of the above-mentioned special lipases comes from N 0 V 0 N 0 RDISKA / S with the trade name of LIP 0 LASE «Others suitable for use here The fat of the shangle value is A η ο η 〇- P fat, AH ο η 〇- B fat, A m ο η ο ('Ε S Ul1 fat, A ra () η ο Λ Κ G fat, PJf雅 ._1 来 Γ.Ι [: i T 0 y 0 J ο z 〇 company's A m ο η 0 pharmaceuticals and the United States 1 丨 S Health 丨 l: the company and NL's D iosynth company is also suitable for commercialization The lipase is for use in Genhan's sister compound of the present invention. Root thumb's sister compound of the present invention generally contains from 0.1 to 1 per gram of M · Yanyan (please read the precautions on the back before writing. Page) Binding-Threading · The size of this paper is in Chinese National Standard (CNS) A 4 specifications (210x297 g: ¢) 315108 Λ 6 Β6 Printed by the Ministry of Economic Affairs, Central Bureau of Industry and Commerce Co., Ltd. V. Description of invention (4 ) 10000 lipolytic units · W per gram of the final product is preferably from 10 to 2500 lipolytic units, the definition of lipolytic units in the example of the European patent application Case No. 0 258 068, page 5, line 38. The protease used in accordance with the present invention is a modified bacterial serine protease. All natural bacterial serine proteases are characterized by an active site necessarily containing one sister and three amino acids of serine , Sister acid and aspartic acid. These amino acids are located in the natural form of the enzyme and have the effect of catalyzing the cleavage of peptide bonds within the protein. Another common point between the bacterial serine proteases in these natural series There is always a methionine tan attached to the active position of serine. According to the present invention, suitable bacterial serine proteases are those in which the methionine attached to the active position of serine has been replaced by another amino acid Protease. The active position serine can also be defined as serine at the same position as serine at position 2 2 1 of the amino acid strain of the bacterial subtilisin protein produced by Bacillus subtilis; the above series are listed hereafter In Figure 1. In the amino acid series of this bacterial subtilisin M protease produced by Bacillus subtilis, methionine is straight After the serine at the 2 2 1 position, the methionine is at the 222 position, and the methionine must be replaced by another amino acid. In other bacterial seric acid protein_series, the egg # ί 时 may not After searching for the active site of β ίί grounding bismuth acid; among the tarps that act as a ladder, the egg town and the amino acid series of the finely closed tortoise M protein fig, which are produced by the tarnishing of the grass, are the first in the amino acid series. 2 2 2 The methionine on the ΙΪ is the same, the egg ® ribipor is replaced by another amino acid ... It is known that the present invention is worse than this without these improved protein saturation itself (please read the back side first Please pay attention to this page and then fill out this page.) Binding and Binding. The paper size is in accordance with Chinese National Standard (CNS) A4 specifications (210x297 g.11) 215108 Λ 6 Β 6 Ministry of Economic Affairs Printed 5. Description of the invention (5) The special application of these improved protein plums to the clears that also contain lipase; therefore, it is not clear how to obtain these improved protein syrups in detail; Or any other basis known in the art of the subject Engineering technology for the & $ role; for example, describes Shaw obtain these proteases GIST-BROCADES Ν.Ζ · In European Patent Application No. 0 328 229. Another method is described in European Patent Application No. 0 32 8 229, which also shows an improved bacterial serine protein suitable for use in the composition according to the invention. Moreover, the partially modified bacterial serine protein plum suitable for use in the sister compound according to the invention has commercial value • such as DURAZYH from NOV0. It is a modified SAVIN AS E of methionine; another acre of useful modified protease A drag example is MAXAPEM 15 from GI5T-BR0CADES, which is a modified MAXACAL modification in which methionine at position 216 has been replaced. Modified OPTICLEAN and OPTIMASE from the SOLVAY enzyme are also effective experimental examples: replacing methionine at position 216 with cysteine improves both. The preferred improved bacterial serine proteases according to the present invention are MAXAPEM 15 from GIST-BROCADES and DUR ￥ ZY Μ from N0V0. The sister compound of the present invention generally contains from 0.5 to 0.5 g per gram of final product. 10 grams of active street white enzymes, with a gram of M final product from 0.01 to 5.0 active protease is preferred, the above-mentioned mixture of fine M silk SS protease modified herein is also suitable for use In the sister compound according to the invention. The rest of the liquid natrium detergent composition based on the present invention is composed of well-known detergent ingredients, namely water, surface active agent 'rib i agent ft ^ · — · —— —-7 -This paper is 5GB in size and is used in the National S National Standard (CNS) 4 specifications (210x297 public goods) (please read the precautions on the back before filling the nest page) Binding · Order-Line · 215108 A 6 B 6 5. Description of the invention (6) Other ingredients, etc. The description of these ingredients under Μ is for the completeness of the description, not to explain the limitation of the sister compound according to the present invention to those well-known ingredients described. The liquid detergent composition herein includes from 5% to 6% by weight of the total liquid detergent composition selected from nonionic, anionic, cationic and zwitterionic surfactants and others The mixture of surfactants is preferably from 10% to 40% by weight. Suitable anionic surfactants are selected from the sulfonate and sulfate group. Suitable anionic surfactants, which are well known in detergent technology, have found wide application in commercial detergents. The alkyl group contained in the molecular structure of the preferred anionic water-soluble sulfonate or sulfate contains from about 8 to about 22 carbon atoms. The Ministry of Economic Affairs of the People ’s Republic of China will print it on the consumer cooperative (please read the back side first. Please fill in this page again) The examples of these preferred anionic surfactant salts are derived from C β -C ^ 8 fatty alcohols such as fat oil, palm oil, palm kernel oil and coconut oil; The alkyl group contains alkyl benzene sulfonic acid from about 9 to about 15 carbon atoms; sodium alkyl glyceryl ether sulfonate; sulfates of fatty alcohols derived from fatty oil and coconut oil and having in alkyl sulfonate Reaction products obtained by sulfation of water-soluble salts of paraffin sulfonates of about 8 to about 22 carbon atoms. It can also be used in the example of US Patent Specification No. 3.3 2 3, 8 8 0, which is more extensively referred to as sulfated olefin table active agent, represented by the well-known ions and ions. Dangerous ions synthesize sulfonate and / or sulfur salt. The cation is widely used in detergent technology, such as sodium, potassium, and alkalium. The water-soluble salts of alkyl sulfonic acid represent the appropriate anionic synthetic surfactant composition in this article. M alkyl sulfonate is preferred, and the alkyl standard is used in the national standard of the paper. CNS) A 4 specifications (210X297 public waste) 21510S Λβ ______ B _ ^ __ V. Description of the invention (7) Alkylbenzene sulfonate with about 10 to .13 sterilized carbon atoms is preferred. Another preferred anionic surfactant in this context is an alkyl sulfonic acid sodium having about 10 to 15 carbon atoms in the alkyl group. Nonionic surfactants suitable for use herein include those that make ethoxylates reactive with hydrogen atoms in the presence of acidic or test catalysts, ie hydrocarbons such as hydroxycarboxy or amine groups Reagents produced by concentration of compounds, and include compounds with the general formula RA (CH2CH20) "H, where R represents the hydrophobic part, A represents the group with reactive hydrogen atoms and η represents the ethylidene gasification part The average value of parts. R usually includes from about 8 to 22 carbon atoms. It can also be formed by the concentration of M-propyl oxide with lower molecular weight compounds. Η usually ranges from about 2 to about 24. Ministry of Economic Affairs Printed by Biyang Standard Industry and Consumer Cooperative (please read the precautions on the back before filling out this page) Κ fatty alcohol with 12 to 15 carbon atoms and from 4 to 10 Mo per mole or per lipase Concentrated products of ethoxylated ethoxylates represent preferred nonionic ethoxylates. Suitable ethoxylates include Ci2-Cls oxymonoalcohols and 3 to 3 9 moles of concentrated products of oxyethylene; C14-C15 Concentrated product of a narrow base of fermented yeast and 3 to 9 moles of ethoxylate per fat (oxygen) alcohol; Cl2-Cl3 oxymonoalcohol fat (oxygen) and Each mole of lipase has 5 volumes of condensed products of ethoxylates and Ci 〇- C i 4 coconut fat age and ethyl terbium are converted into grades from 4 Φ: b (次. 乙 钻Molar number of oxides / fatty acid of 1 mol). It is based on process conditions and olefin raw materials. In most of the linear grease-proof gas-based drunks may have short chains, such as It is a branched-chain methyl group. Changfa intends to use the Chinese National Standard (CNS) for the 5fc scale of the original paper in the fan garden where the degree of branching in the business is from 5 to 50% (K spray meter) A4 specifications (210x297 male dragon) Λ (ί η 6 V. Description of the invention (8) Common cationic surfactants include quaternary ammonium compounds with the chemical formula hRzHiT, where Ra, R2ftR3 are methyl groups, but (: 12 -(: 15 of the comprehensive group, or here Ri is ethyl or ethyl group · R2 and R3 are methyl, and 1? 4 is Cl2-Cl5 of the hospital group. Zwitterionic surfactant package Derivatives of quaternary ammonium, phosphonium and chemical compounds of fatty strips, the fatty part may be linear or branched; and one of the fatty substitutions includes from about 8 to about 24 carbon atoms and another substitution It includes at least one anionic group that dissolves in water. The other excellent zwitterionic material is U.S. Patent Application No. 3,925.262 issued by Laughlin (Laughlin on December 9, 1975 and by Lafolin et al. In丨 Ethoxylated ammonium sulfonate and ammonium sulfate shown in US Patent Application No. 3,929.678 published on December 30, 975. The water-soluble amine oxides included in semi-polar nonionic surfactants contain a false The alkyl or hydroxyalkyl part of 8 to about 28 carbon atoms and the two parts are selected from the group consisting of alkyl and hydroxyalkyl groups containing from 1 to about 3 carbon atoms, and these groups can be selected as needed Incorporated into the ring structure. The polyhydroxy fatty acid amide surfactant with the chemical formula of ^^-is also suitable for 0 R 1.. !! Γ_ 屮 火 Ι ΐ: 舀 Λ ί \ i- w1] fi, if WH in K 1 foot 1 丨, (: Hydrocarbon dip, 2 湫 杫 乙 丛, 2 mixture of spermyl or other # mixture, R 2 is a C β _ Ί i hydrocarbon snail, and ζ is a linear hydrocarbon with a minimum of 3 hydroxyl groups directly in the The polyhedrons of h, or the other gas-retaining derivatives, preferably R i is a radical, r; ί is a straight-bonded shuttle or phenanthrene or their port complex, and ζ stapes Derived from the original seal in Zhankan --------------_ 10-This standard is used in the standard S ® home 4JiMCNS) ΤΜ standard (2 丨 0x297 public) Λ 6 Β6 215108 V. Description of the invention (9 ) (Please read the precautions on the back before filling out this page) Amidation reaction. The raw sugar in the reaction, such as glucose, fructose, maltose, lactose, etc. The composition based on the present invention generally contains a rib lotion system . Any well-known builder system is suitable, but is preferably a mixture of citric acid and substituted succinic acid. The citraconic acid rib lotion used in the examples of the present invention will be present in the final product in the form of any water soluble salt of citraconic acid. For example, these salts include sodium, potassium, ammonium or alkanoammonium salts. In fact, it is convenient to use the clavulanic acid monohydrate mud that will be neutralized in situ as the starting material M to facilitate the formation of the above-mentioned species. The succinic acid builders substituted herein have the general formula r-ch (cooh) ch2 (cooh), that is, a derivative of succinic acid, where R is a c1〇-cie alkyl or alkenyl group, with [ : 12-(: 14alkenyl is preferred. These substituted succinic rib lotions in the final product are preferably K in the form of their water-soluble salts, including sodium, potassium, ammonium or alkanolammonium Salts (ie mono-, di- or tri-ethanolammonium). It is believed that it is better to use these succinic acid derivatives in their diacid or anhydride form. The diacid will be neutralized in situ and the anhydride will The process of hydrolysis / neutralization is carried out. ^ Central Ministry of Economic Affairs: ^ ::: Gongxiao ^ Cooperative Society ^ W Special T-ribbed Washing Example Q Μ I. Alkyl saccharine, ten Tetraxane is called succinic acid, hexadecyl succinic acid, 2-dodecene S butadiene (preferred), 2-tetradecenyl succinic acid and the like. The preferred rib lotion system contains The total response of Κ total sister compound ranges from 4% to 12% of the above-mentioned substituted succinated rib lotion and K 缌 sister compound "from -1 1-each recognition scale is used t 3 a Domestic fraud standard (CNS) Xuan 4 specifications (210x297) Λ 6 B 6 , Description of the invention (i〇) 'll 4% to include fat is good. From the performance of this material has to 8.0 Μ 本. Shi Jing SS natural its clear and well-known, 1, 1.2% twisted acid is considered It is necessary to know that there will be a significant acid test value. The invention is based on the fact that the composition of the lipase cleanser of this book is well known. 2-Propylene system generally exists betulinic acid. The sister compound based on the present invention can also be included It is necessary to choose the builder. The skills of oleic acid and palm oleic acid know that the acid value of the sister compound can affect the enzyme system. Therefore, the sister combination based on the present invention will be adjusted to It is preferably in the range from 6 to 10, and the sister compound according to 7.5 may also contain an enzyme stabilization system. The protease in the system provided by the invention will not significantly attack ... but the enzyme system The system or composition may still be subject to instability issues. Therefore, stabilizers in this technique may be required. The preferred enzyme stabilizer is selected from boric alcohols, carboxylic acids, and mixtures of these enzymes. The weight of the total sister compound ranges from 0.01% to 5% (please read the note on the back first Please fill in this page for more details.) The sister compound of the present invention can also contain other enzymes, such as cellulase and lysozyme. The amylase in the presence of protease seems to be different, therefore It is better that the substance M contains amylase. -Ν \ 撗 济 $ -Central Standard Χ, ΙΗ 工 消 1 'Cooperative Society imprinted in Hongmu can include a system of more suitable additives that can be selected according to needs夜 例 包 坫 肥 @ 水 rnsu 1 an ls, sunscreen, reagents to improve the mechanical suitability of enamel-coated surfaces, bactericides, dyes, bleach, bleach containing perborate and percarbonate Brightener, h-pot release agent, cooking agent and the like. The liquid sister compound in Muwen can be used as an additive to dry cumen, which is generally used in the 12-sheet paper. The standard is S. Fibonacci (CNS) Τ 4 specifications (210 X 297 Gong Ji) Λ 6 B 6 5. Description of the invention (ll) Amount from 0.0 5 to 5%. These additives include polyamino carboxylates, such as the water solubility test of ethylenediamine tetra-acid, secondary ethyl triamine penta-acid, ethylene diamine di-succinic acid or their Jin Yu. Other additives include organic phosphonic acids; particularly preferred are methinediaminotetramethylenephosphonic acid, hexamethylenediaminotetramethylenephosphonic acid, and secondary ethyltriaminopentamethylene Phosphonic acid and aminotrimethylphosphonic acid. The listed ingredients are combined in the proportions shown to prepare the following compounds according to the present invention. (Please read the precautions on the back and then fill out this page) Shibu Shiji, the Ministry of Economic Affairs, advises Ugong Consumer Co., Ltd., this standard is to be used in the S 3 standard (CNS) A.1 specifications (210x297 ) 51 2l5i〇8 Λ 6 B 6
V i'·'·-濟郎屮央捣準乃以工消费合作社印製 五、發明説明（丨2 ) 1 2 3 4 5 —線性烷基苯磺酸鹽 12 7 6 7 8 一 C12-C1S烷基磺酸納 2 2 3 3 2 —(：14-(：15烷基以2.5次的 0 0 2 2 0 乙氧基化之硫酸鹽 一 C12葡萄糖醯胺 0 0 6 6 0 —C12-C15醇以7次乙氧基化 8 0 0 0 0 _(：12-(：15醇以5次乙氧基化 0 8 0 0 8 γ油酸 一槔樺酸 2 0 0 - 0 0 3 9 9 13 15 —(：12-(：14烯基取代丁二酸 10 5 5 7 6 —乙醇 4 4 3 4 5 一 1，2 —丙二醇 2 3 3 1 2 -NaOH 6 8 8 11 11 一二次乙基三胺五（次甲基膦酸） 0.5 0.7 0.7 1 1 —澱粉_ ( 143 KNU/克） 0.1 0,1 0.05 0.2 0.1 —Lipolase (100 KLU/克商業溶液） 0.4 0.2 0.3 0.3 0.3 -PRM15 (50¾克/克商業溶液） 0.3 0 0 0 0.4 I) u r a /, .y in ( Λ / Λ 构 ΐϊ 浴叔） 0 0 . ^ 0 0 0 — Optic lean M222C (賁驗樣品） 0 0.1 0 0.4 0 - Opt: i mase M222C (宵驗樣品） 0 0 0.3 0 0 -CaCL· 0.01 0 0.01 0.01 0.02 -Na偏硼峙辣 2.2 2 •1 4 3 --ΊΈΑ - 14 0 0 0 0 0 (請先閱讀背面之注意事項再填寫本頁) 本紙張尺度边用中a S家4ΐ準（CNS) 1丨規格（210x297公及） 濟 部 屮 央 準 X;V i '·' · -Jilang Panyang was printed by the Industrial and Consumer Cooperatives. Fifth, description of the invention (丨 2) 1 2 3 4 5 —Linear alkylbenzene sulfonate 12 7 6 7 8 —C12-C1S alkyl Sodium sulfonate 2 2 3 3 2 — (: 14-(: 15 alkyl with 2.5 times of 0 0 2 2 0 ethoxylated sulfate mono-C12 glucosamine 0 0 6 6 0 —C12-C15 alcohol with 7 times ethoxylation 8 0 0 0 0 _ (: 12- (: 15 alcohol with 5 times ethoxylation 0 8 0 0 8 γ oleic acid and betulinic acid 2 0 0-0 0 3 9 9 13 15 — (: 12-(: 14alkenyl substituted succinic acid 10 5 5 7 6 —ethanol 4 4 3 4 5 -1,2-propanediol 2 3 3 1 2 -NaOH 6 8 8 11 11 Amine penta (methine phosphonic acid) 0.5 0.7 0.7 1 1 — starch_ (143 KNU / g) 0.1 0,1 0.05 0.2 0.1 —Lipolase (100 KLU / g commercial solution) 0.4 0.2 0.3 0.3 0.3 -PRM15 (50¾ g / G commercial solution) 0.3 0 0 0 0.4 I) ura /, .y in (Λ / Λ constitutive ϊ bath uncle) 0 0. ^ 0 0 0 — Optic lean M222C (test sample) 0 0.1 0 0.4 0-Opt : i mase M222C (night test sample) 0 0 0.3 0 0 -CaCL · 0.01 0 0.01 0.01 0.02 -Na partial boron is hot 2.2 2 • 1 4 3 --ΊΈΑ-14 0 0 0 0 0 (Please read the precautions on the back before filling in this page) This paper is used in the borders of a S home 4 standards (CNS) 1 丨 Specifications (210x297) and the Ministry of Economic Affairs X;
A 工 W1! 合 作 印 ϋΐ Λ 6 B 6 五、發明説明（13) 一甲酸納 0 0 0 0 0 一脂肪酸 0 0 0 0 0 -Λ/ Vr /\y A sif m 4½ 一· « c=t 2S 1 λ λ ny ，J、久 / 里 WJ 厲 τα Tffi 土 i V u 將所列的成份依陳列的比例混合以製備根據本發明的下列姐合物。 70 * 6 7 8 9 10 一線性烷基苯磺酸鹽 5 7 9 8 10 —C12-C15烷基磺酸納 5 2 1.75 0 3 —(：14-(：15烷基以2.5次的 2 0 2 0 0 乙氧基化之硫酸鹽 一 C12葡萄糖醯胺 6 0 7 0 0 — C12_C15醇以7次乙氧基化 8 0 * 0.5 0 11.6 一 Ci2-C15酵以5次乙氧基化 0 8 0 8 一油酸 0 0 0 3.5 2.5 一擇樣酸 10 9 9.5 4 1 一Cu-Cw烯基取代丁二酸 11 0 1K5 0 0 —STPP 0 20 0 0 0 (# ί ι 0 0 0 Z() 0 乙龄 6 4 4 3 6 --1，2 —丙二龄 3 2 2 2 1.5 --N a 0 Η 9 9 9.8 9 3.5 一 —·.次乙褡三胺κ (次甲胡膦种') 1.0 1.0 1.0 0.5 0.8 —澱粉酶（1，丨3 KNU/克） 0.2 - 15 0. I 0.2 0.05 Ί 本紙悵尺度边用中S國家櫺準（CNS) T4規格（210x297公!1) (請先閲讀背面之注意事項再填寫本頁)A Gong W1! Cooperation seal ϋ Λ 6 B 6 V. Description of the invention (13) Sodium formate 0 0 0 0 0 0 Fatty acid 0 0 0 0 0 -Λ / Vr / \ y A sif m 4½ one «c = t 2S 1 λ λ ny, J, Jiu / Li WJ Li τα Tffi Tu i V u The listed ingredients are mixed in the proportions shown to prepare the following sister compounds according to the present invention. 70 * 6 7 8 9 10 linear alkylbenzene sulfonate 5 7 9 8 10 —C12-C15 sodium alkylsulfonate 5 2 1.75 0 3 — (: 14- (: 15 alkyl with 2.5 times of 2 0 2 0 0 Ethoxylated sulfate mono-C12 glucosamine 6 0 7 0 0 — C12_C15 alcohol is ethoxylated 7 times 8 0 * 0.5 0 11.6 A Ci2-C15 enzyme is ethoxylated 5 times 0 8 0 8 Monooleic acid 0 0 0 3.5 2.5 One-selected acid 10 9 9.5 4 1 Cu-Cw alkenyl substituted succinic acid 11 0 1K5 0 0 —STPP 0 20 0 0 0 (# ί ι 0 0 0 Z ( ) 0 AGE age 6 4 4 3 6 --1,2-propanediol age 3 2 2 2 1.5 --N a 0 Η 9 9 9.8 9 3.5 —— ·. Hypoethylene triamine κ ') 1.0 1.0 1.0 0.5 0.8 —amylase (1, 3 KNU / g) 0.2-15 0. I 0.2 0.05 Ί This paper is used for the standard side of China National Standards (CNS) T4 specification (210x297 g! 1) ( (Please read the notes on the back before filling this page)
Λ 6 Β6 -山人忙準X,IU 工;/1"+合ίί;社. 五、發明説明（i4) 一 Lipolase (10〇 KLU/克商業溶液） 0.5 0· 5 0.3 0.2 0.3 —PEM15 (50毫克/克商業溶液） 0.4 0 0 0 0.2 一 Durazyn (39毫克/克商業溶液） 0 0 0.5 0 0.2 —Opticlean M222C (實驗樣品） 0 0 0 0.3 0 —Opt i mase M222C (實驗樣品） 0 0. 5 0 0 0 —CaC 12 0.01 0. 01 0.02 0.02 0.01 一 Na偏硼酸鹽 4 2 4 3 0 -TEA 0 0 0 0 6 一甲酸納 0 0 0 0 1 一脂肪酸 0 0 0 0 12 一水及少量副靨物 - --補 足 至 100 %----- — 將所列的成份依陳列的比例混合以製備根據本發明的下列姐合物。 11 12 13 14 15 一線性烷基苯磺酸鹽 5 7 9 8 10 一 C 1 2 - 1 b综蟇確酸納 5 2 1.75 0 3 2 0 2 0 0 乙氣丨ί- ib之&ΐ般者3 一 Cu關萄槠醯胺 6 0 7 0 0 — 7次乙氧葫化 8 0 0.5 0 11.6 一1：12-1;,恶以5次乙氧基ib 0 « 0 3 油鹊 0 0 0 3.5 2.5 —Γ4 1¾ 10 9.5 4 1 — 16 - (請先閱讀背面之注意事項再塡寫本頁) 本紙張尺度边用中围Κ家標準（CNS) Μ規格（210 X 297公) 裝· 訂Λ 6 Β6-Shanren Busy X, IU Engineering; / 1 " + 合 ίί ;. V. Description of the invention (i4) A Lipolase (10〇KLU / g commercial solution) 0.5 0 · 5 0.3 0.2 0.3 —PEM15 ( 50 mg / g commercial solution) 0.4 0 0 0 0.2 Durazyn (39 mg / g commercial solution) 0 0 0.5 0 0.2 —Opticlean M222C (experimental sample) 0 0 0 0.3 0 —Opt i mase M222C (experimental sample) 0 0 . 5 0 0 0 —CaC 12 0.01 0. 01 0.02 0.02 0.01 mono Na metaborate 4 2 4 3 0 -TEA 0 0 0 0 6 sodium formate 0 0 0 0 1 mono fatty acid 0 0 0 0 12 monohydrate and A small amount of deputy substance--make up to 100% ------mix the listed ingredients in the proportions shown to prepare the following sister compound according to the present invention. 11 12 13 14 15 linear alkylbenzene sulfonate 5 7 9 8 10 mono C 1 2-1 b sodium oxalate 5 2 1.75 0 3 2 0 2 0 0 ethane Ordinary 3 a Cu-related guanamine 6 0 7 0 0 — 7 times ethoxylated 8 0 0.5 0 11.6 a 1: 12-1 ;, with 5 times ethoxy ib 0 «0 3 oil magpie 0 0 0 3.5 2.5 —Γ4 1¾ 10 9.5 4 1 — 16-(Please read the precautions on the back before writing this page) This paper size is installed in the Zhongwei K Family Standard (CNS) M specifications (210 X 297) · Order
Si Λ 6 Β6 五、發明説明（15) 濟 部 十 央 準 X； Π 工 合 社 卬 —C12-14烯基取代丁二酸 11 0 11.5 0 一酒石酸鹽單丁二酸鹽 0 15 0 17 一二乙氧基化聚（1，2—次丙 1.0 0.5 0.7 0 基對笨二酸鹽> 一乙醇 6 4 4 3 —1，2 —丙二醇 3 2 2 2 -NaOH 9 9 〆 9.8' 9 一二次乙基三胺五（次甲基膦酸） 1.0 1.0 1.0 0 一澱粉酶（143 KHU/克） 0.2 0.1 0.2 0 一 Lipolase (100 KLU/克商業溶液） 0.5 0.5 0.3 0 一 PEM15 (50毫克/克商業溶液） 0.4 0 0 0 —DU「azym (39毫克/克商業溶液） 0 0 0.5 0 —Opticlean M222C (實驗樣品） 0 0 0 0 —Optimase M222C (宵驗樣品） 0 0.5 0 0 —CaC 12 0.01 0.01 0.02 0 -Na偏硼酸翻 4 2 4 3 -TEA 0 0 0 0 --甲酸訥 0 0 0 0 -脂肪酸 0 0 0 0 水及少迠丨別祕物 h\ fL 个 100 % ,02 ο ο 2 6 ο ο ο 2 (請先閲讀背面之注意事項再填寫本頁) 装· 訂 17 本紙;k尺度边用中S 83家標準（CNS)甲4規格（210x297公及）Si Λ 6 Β6 V. Description of the invention (15) Shibu quasi-X of the Ministry of Economy; Π Gongheshe—C12-14 alkenyl substituted succinic acid 11 0 11.5 0 monotartrate monosuccinate 0 15 0 17 one two Ethoxylated poly (1,2-times propylene 1.0 0.5 0.7 0 base paradibenate> monoethanol 6 4 4 3 —1,2-propanediol 3 2 2 2 -NaOH 9 9 〆9.8 '9 one two Phenylenetriamine penta (methinephosphonic acid) 1.0 1.0 1.0 0 One amylase (143 KHU / g) 0.2 0.1 0.2 0 One Lipolase (100 KLU / g commercial solution) 0.5 0.5 0.3 0 One PEM15 (50 mg / G commercial solution) 0.4 0 0 0 —DU “azym (39 mg / g commercial solution) 0 0 0.5 0 —Opticlean M222C (experimental sample) 0 0 0 0 —Optimase M222C (night test sample) 0 0.5 0 0 —CaC 12 0.01 0.01 0.02 0 -Na metaboric acid 4 2 4 3 -TEA 0 0 0 0 -Formic acid 0 0 0 0 -Fatty acid 0 0 0 0 Water and Shao shu 丨 other secrets h \ fL 100%, 02 ο ο 2 6 ο ο ο 2 (Please read the precautions on the back before filling in this page) Binding · Order 17 copies of paper; k-size side use S 83 home standard (CNS) A 4 specifications (210x297)