EP0397788A1 - Dispositif de melange de solutions - Google Patents

Dispositif de melange de solutions

Info

Publication number
EP0397788A1
EP0397788A1 EP89902577A EP89902577A EP0397788A1 EP 0397788 A1 EP0397788 A1 EP 0397788A1 EP 89902577 A EP89902577 A EP 89902577A EP 89902577 A EP89902577 A EP 89902577A EP 0397788 A1 EP0397788 A1 EP 0397788A1
Authority
EP
European Patent Office
Prior art keywords
mixing
chamber
head
mixing chamber
area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP89902577A
Other languages
German (de)
English (en)
Other versions
EP0397788A4 (en
Inventor
Bruno Michel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applied Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems Inc filed Critical Applied Biosystems Inc
Publication of EP0397788A1 publication Critical patent/EP0397788A1/fr
Publication of EP0397788A4 publication Critical patent/EP0397788A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/27—Mixers with stator-rotor systems, e.g. with intermeshing teeth or cylinders or having orifices
    • B01F27/272—Mixers with stator-rotor systems, e.g. with intermeshing teeth or cylinders or having orifices with means for moving the materials to be mixed axially between the surfaces of the rotor and the stator, e.g. the stator rotor system formed by conical or cylindrical surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F2025/91—Direction of flow or arrangement of feed and discharge openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F2025/91—Direction of flow or arrangement of feed and discharge openings
    • B01F2025/911—Axial flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis

Definitions

  • the present invention deals with a device for the mixing of solutions with a mixing chamber having minimally two input lines and an exit line.
  • deadtime re., time between mixing and start of the analysis, required is minimally 5 seconds.
  • a reduction of the total analysis volume and the frequently very expensive liquids is not realizable by manual mixing.
  • Known devices for the mechanical mixing of liquids consists, for example, of two syringes each filled with a liquid whose contents are simultaneously injected into a mixing chamber, mixed based on the resulting turbulence, and subsequently transfered to a cuvette.
  • the deadtime can be reduced to less than 5 mill- seconds.
  • the liquid must be injected into the mixing chamber under relatively high pressure. Due to the high back pressure in the mixing chamber the titration accuracy is reduced which is quite detremental when different liquids and large volume differences must be mixed. Therefore it is not possible to mix volume differences of 1 :50 with sufficient accuracy.
  • the task of the present invention is to create a device for the fast mixing of small amounts of liquids with quite different viscosities. This task • has been solved by the features of the first patent claim.
  • the essential advantages of this invention are that because of the active mixing principal no high injection pressures of liquids are required, which guarantees basically constant titration accuracy whereby very high mixing ratios (up to 1 :2000) are achieved. Due to the proposed cleft shear flux liquids with quite different viscosities can be mixed without problem. The location of the mixing head in the mixing chamber, as proposed in the invention, ensures moreover a small dead volume which has a desirable effect on analysis cost of expensive substances.
  • FIG. 1 Schematic presentation of a preferred version of the mixing chamber of the device according to the invention
  • Fig. 2a and 2b the progress of the shear flux in first mixing area
  • FIG. 3 Cross sectional view through the preferred version of the device according to the invention.
  • FIG. 4a and 4b axial and radial sectional views of a second cuvette
  • Fig. 6 The connection of the mixing device with matching controller.
  • Fig. 1 shows schematically the principal arrangements of this invention.
  • a mixing head (2) built in such a way that it can be propelled around an rotational axis (R) and with the inner wall of the mixing.
  • chamber (1) a
  • the inside of the mixing chamber (1 ), as well as the mixing head (2), are, as shown in Fig. 1 , constructed as coaxial vertical cylinders. Other forms such as cones, partial cones or any , other rotationally symmetric configuration are also applicable.
  • the mixing chamber (1 ) has at one end, for example, four feeding lines (11 , 12, 13, 14) through which different liquids (by enzyme kinetic determinations these are enzyme, substrate, buffer and activator/inhibitor) in the direction of the arrows A, B, C, D continuously inject into the first mixing area (3).
  • the feed lines (11 , 12, 13, 14) enter preferably mixing chamber (1) all at the same height and evenly spaced around the circumference.
  • the injection pressure creates an axial flux (arrow E) which is based on the rotation of the mixing head (2) a tangential flux is overlaid.
  • the overall result is a shear flux in the cleft mixing area (3), in which, for example, one injected volume element through line 11 mainly on spiral movement (24) around the mixing head (2) proceeds and
  • Fig. 2a shows an enlarged axial section through the entrance part of the mixing area (3).
  • a predetermined volume element (21) through line 11 injected liquid shows approximately an axial flow cross section (22).
  • Fig 2b shows a respective radial cross section through the entrance part of the mixing area (3) to illustrate a momentary tangential cross section (23) of the volume element (21 ).
  • the described hollow cylinderical shear flux in the first mixing area (3) slows the flux minimally, therefor the liquids can be injected with low pressure unlike the known passive mixing devices.
  • the injected liquids can be precisely controlled in volume proportions up to 1 :2000 with enough accuracy. Because the mixing procedure in the entire
  • mixing area occurs evenly, mixtures of liquids of different viscosity and consistency can be produced without problems.
  • the arrangement of the mixing head (2) inside mixing head (1) helps to keep the dead volume of the entire device very small.
  • the axial expansion of mixing head (2) is preferably smaller than mixing chamber (1) so that a second mixing area (4) is created in which there already exists a turbulent flux caused by the rotation of mixing heads (2), in turn, causes additional mixing of the different liquids.
  • the bottom of mixing head (2) has special projections, the bottom (6) of mixing chamber (1 ) has furrows.
  • an exit hole (15) through which the liquid exits the mixing chamber (1). It is understood that the mixing chamber can be equipped with multiple exit holes.
  • Fig. 3 shows the arrangement of the previously described mixing chamber in the system (10).
  • the inner case (42) contains a motor (32) and a coupled drive magnet (31).
  • the mixing head (2) is, for example, a magnet covered with an inert material which is driven on the same rotational axis (R) by another magnet (31). Through the pull of the drive magnet (31) the magnetic mixing head (2) is pulled to the top of the mixing chamber (1) thereby forming a thin liquid film between the mixing head and the cover which serves as a lubricant.
  • thermostated chamber (43) Between the outer cover (41) and the insulated inner chamber (42) is a thermostated chamber (43) and the liquids for mixing are transported through a connecting piece (44) into the thermostated chamber (43) which is filled with thermostated water to mixing chamber (1) (illustration only shows a single feed line 11).
  • the thermostated water acts simultaneously as cooling for the heat produced by motor 32.
  • the mixture exits through exit 15 and proceeds via a connecting piece 52 to a
  • cuvette 51 Different physical and chemical parameters of the mixture can be measured in cuvette (51) with usual analytical instruments (ie., by optional and electrical procedures).
  • the mixture flows through the cuvette (51) and goes via another connector (53) and a line (54) through the thermostated chamber (43) to a second cuvette (62) in which an addition measurement can be made with an additional measuring element (61 ).
  • the mixture exits through a tube (16) and the connector 44.
  • connectors (52 and 53) many different cuvettes (51) can be inserted into the outer shells (41 ).
  • Fig. 4a shows an axial cross section
  • Fig 4a a radial cross section through the outer cover (41) and the connected cuvette (62).
  • a hole (64) is provided for an exchangeable measuring probe (61) (compare with Fig. 3). By putting the probe (61) in place the hole (64) will be closed so that the mixture passing through feeding line (54) and a connector (63) to cuvette (61) exits the opening (64) through a lateral opening (65).
  • the volume of the second mixing area (4) corresponds preferably with the one of cuvette (51). This creates a buffer zone in which a homogeneous mixture is produced even by non-continuous additions of small amounts of liquids.
  • an array of mixing heads are planned that can be simply exchanged by opening the mixing chamber (1) (ie., removal of the base (6), as shown in Fig. 3, is part of the screwed together outer case 41).
  • opening the mixing chamber (1) ie., removal of the base (6), as shown in Fig. 3, is part of the screwed together outer case 41.
  • axial (R) is vertical manner. It does not matter which part of the device is on top.
  • the chosen illustration in the figures is random because the flux of the liquids to the device is independent of gravity but is a matter of the injection pressure.
  • the proposed mixing device is especially useful for the automatic analysis
  • FIG. 5 shows a possible arrangement in which the proposed mixing device (10), including a cuvette (51 ), are put into a conventional analytical instrument (101 ).
  • the proposed device can be designed in such a way that the use of conventional instruments is possible.
  • the liquids of mixing come from a control unit (102), through the different feed lines inside of a surrounding tube (71 ), to connector piece (44) of the device (10).
  • the control unit (102) has a sample chamber (105) from which the liquids are loaded by sucking syringes.
  • the barrels of the syringes are equipped with, for example, stepper motors so that for each measurement the required liquid amount is exactly delivered through tube (71) into the mixing chamber (1 ) of the device (10).
  • Sample chamber (105) can be additionally equipped with an autosampler so that one of the components can be automatically changed with each series of measurements.
  • the analysis is controlled and results analyzed with a computer (104) equipped with the respective peripheral
  • An instrument (103) provides the previously mentioned thermostated water.
  • Fig. 6 shows the backside of the control unit (102) with a flexible tube (71) to mixing device (10).
  • a flexible tube (71) to mixing device (10).
  • the four feeding lines (11 , 12, 13, 14) the return (16) for the analyzed mixture, an electrical connecting piece (108) for temperature sensor (not shown), power line (109) for the drive motor (32), as well as a feeder line (17) for delivering thermostated water to the thermostated chamber (43).
  • the return flow of the thermostated water in line (71) preferably occurs in an open manner such that the liquids in the feeding lines are already surrounded by thermostated water. This provides a very accurate temperature control of the components.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Accessories For Mixers (AREA)

Abstract

Un dispositif permettant le mélange de liquides comprend une chambre de mélange (1) dans laquelle est disposée une tête de mélange mue autour d'un axe. Entre la paroi de la chambre de mélange et la tête de mélange (2) est créée une zone de mélange fendue. La rotation de la tête de mélange (2) crée un flux de cisaillement des liquides injectés (11, 12, 13, 14), ce qui provoque le mélange. Avec ce procédé de mélange actif, on peut mélanger avec une rapidité élevée des quantités minimes de liquide, de volumes et de viscosités différents. Le dispositif est particulièrement adapté à l'analyse automatique de la cinétique des enzymes.
EP19890902577 1988-01-29 1989-01-27 Device for mixing solutions Withdrawn EP0397788A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH321/88A CH674317A5 (fr) 1988-01-29 1988-01-29
CH321/88 1988-01-29

Publications (2)

Publication Number Publication Date
EP0397788A1 true EP0397788A1 (fr) 1990-11-22
EP0397788A4 EP0397788A4 (en) 1991-07-10

Family

ID=4184354

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19890902577 Withdrawn EP0397788A4 (en) 1988-01-29 1989-01-27 Device for mixing solutions

Country Status (4)

Country Link
EP (1) EP0397788A4 (fr)
JP (1) JPH03503137A (fr)
CH (1) CH674317A5 (fr)
WO (1) WO1989007006A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4628489A (en) * 1988-11-08 1990-05-28 Applied Biosystems, Inc. Assayomate
EP0734798A1 (fr) * 1995-03-28 1996-10-02 R. LISCIANI TRAFILERIE E DIVISIONE DYN AUTOMAZIONE INDUSTRIALE S.n.c. Machine à terfiler pour fil métallique lubrifié à sec
JP4782426B2 (ja) * 2003-03-21 2011-09-28 ケミラ オユイ 乳濁液または分散液を連続的に作製するための装置および方法
WO2006109741A1 (fr) * 2005-04-08 2006-10-19 National University Corporation Okayama University Dispositif et procede de generation d'ecoulement mixte
JP5164790B2 (ja) * 2008-06-24 2013-03-21 キヤノン株式会社 分散体の製造方法および液体混合装置
JP2023515142A (ja) * 2020-02-25 2023-04-12 ヘリックスバインド・インコーポレイテッド 流体システムのための試薬キャリア

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2973946A (en) * 1958-07-29 1961-03-07 American Viscose Corp Mixing apparatus
DE1457182A1 (de) * 1965-07-26 1968-12-12 Wacker Chemie Gmbh Verfahren zum kontinuierlichen Mischen
FR1563514A (fr) * 1967-05-19 1969-04-11
US3752444A (en) * 1971-08-03 1973-08-14 P Foucault Apparatus for mixing fluids
EP0132169A1 (fr) * 1983-06-14 1985-01-23 Saint Gobain Vitrage International Procédé et dispositif pour la fabrication par coulée d'une couche optiquement homogène transparente à partir d'un mélange de composants
EP0191338A2 (fr) * 1985-02-14 1986-08-20 Werner & Pfleiderer GmbH Procédé et dispositif pour l'alimentation contrôlée de concentrés de couleurs dans des machines à hélices

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US2243309A (en) * 1938-11-25 1941-05-27 Mining Process & Patent Co Flotation apparatus
US2513562A (en) * 1942-06-06 1950-07-04 Colgate Palmolive Peet Co Method and apparatus for thermally regulating gravimetric flow of liquids
US2843169A (en) * 1954-08-04 1958-07-15 Frederick W Stein Laboratory mill for comminuting materials to fine particle size
US3212128A (en) * 1963-03-20 1965-10-19 Air Prod & Chem Mold filling apparatus
US3362919A (en) * 1965-02-04 1968-01-09 Pittsburgh Plate Glass Co Process for foaming thermoset organic materials
US3420506A (en) * 1967-04-03 1969-01-07 Mobay Chemical Corp Mixing apparatus
US3433465A (en) * 1967-05-29 1969-03-18 Roman Szpur Magnetic mixing and stirring device
US4140299A (en) * 1974-07-04 1979-02-20 Imperial Chemical Industries Limited Mixing liquids
US3972614A (en) * 1974-07-10 1976-08-03 Radiometer A/S Method and apparatus for measuring one or more constituents of a blood sample
US4174907A (en) * 1975-06-09 1979-11-20 Massachusetts Institute Of Technology Fluid mixing apparatus
US4390283A (en) * 1979-09-04 1983-06-28 Beckman Instruments, Inc. Magnetic strirrer for sample container
US4357110A (en) * 1979-09-17 1982-11-02 Hope Henry F Mixing apparatus
DE3301043C2 (de) * 1982-02-09 1986-10-23 Akzo Gmbh, 5600 Wuppertal Mischvorrichtung
US4403866A (en) * 1982-05-07 1983-09-13 E. I. Du Pont De Nemours And Company Process for making paints
FR2536298B1 (fr) * 1982-11-18 1986-03-28 Boiron Lab Sa Appareil de dilution, notamment pour la fabrication de medicaments homeopathiques
US4496244A (en) * 1983-01-17 1985-01-29 General Signal Corporation Small volume mixing and recirculating apparatus
JPH0785773B2 (ja) * 1985-12-04 1995-09-20 コニカ株式会社 粉粒体の分散装置
US4720998A (en) * 1986-06-13 1988-01-26 Hogue James D Crude oil sampling system
JPH0638028A (ja) * 1992-07-21 1994-02-10 Fuji Xerox Co Ltd 画像読取装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2973946A (en) * 1958-07-29 1961-03-07 American Viscose Corp Mixing apparatus
DE1457182A1 (de) * 1965-07-26 1968-12-12 Wacker Chemie Gmbh Verfahren zum kontinuierlichen Mischen
FR1563514A (fr) * 1967-05-19 1969-04-11
US3752444A (en) * 1971-08-03 1973-08-14 P Foucault Apparatus for mixing fluids
EP0132169A1 (fr) * 1983-06-14 1985-01-23 Saint Gobain Vitrage International Procédé et dispositif pour la fabrication par coulée d'une couche optiquement homogène transparente à partir d'un mélange de composants
EP0191338A2 (fr) * 1985-02-14 1986-08-20 Werner & Pfleiderer GmbH Procédé et dispositif pour l'alimentation contrôlée de concentrés de couleurs dans des machines à hélices

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO8907006A1 *

Also Published As

Publication number Publication date
WO1989007006A1 (fr) 1989-08-10
JPH03503137A (ja) 1991-07-18
CH674317A5 (fr) 1990-05-31
EP0397788A4 (en) 1991-07-10

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