EP0187167A1 - Appareil doseur pour liquides - Google Patents

Appareil doseur pour liquides Download PDF

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Publication number
EP0187167A1
EP0187167A1 EP84115676A EP84115676A EP0187167A1 EP 0187167 A1 EP0187167 A1 EP 0187167A1 EP 84115676 A EP84115676 A EP 84115676A EP 84115676 A EP84115676 A EP 84115676A EP 0187167 A1 EP0187167 A1 EP 0187167A1
Authority
EP
European Patent Office
Prior art keywords
vessel
measuring device
dosing
metering
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP84115676A
Other languages
German (de)
English (en)
Other versions
EP0187167B1 (fr
Inventor
Winfried Dr. med. Stöcker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP19840115676 priority Critical patent/EP0187167B1/fr
Priority to DE8484115676T priority patent/DE3480383D1/de
Publication of EP0187167A1 publication Critical patent/EP0187167A1/fr
Application granted granted Critical
Publication of EP0187167B1 publication Critical patent/EP0187167B1/fr
Expired legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

Definitions

  • the present invention relates to a device for receiving and storing or dispensing liquids one or more times, which consists of a measuring device (pipette) which does not come into contact with these liquids and an exchangeable metering vessel, the filled metering vessel being able to be separated from the metering device. without it emptying itself in an uncontrolled manner.
  • a measuring device pipette
  • capillary piston pipettes work without air cushions.
  • the liquid is sucked into a capillary by a piston, both are wetted by the liquid.
  • These devices dispense more precisely than the pipettes with air cushions, but they have the disadvantage that the wetted parts are usually not replaced after each sample because of the expenditure of time and the material costs. It is accepted that liquid will be carried over from one sample to the next.
  • DT-OS 26 11 060 describes a dosing "element" which, after filling, could be separated from the pipette without becoming detached Contents emptied:
  • a dosing "element” there is a liquid displacement medium, the volume of which is reduced in the dosing "element” by a measuring device in order to absorb the liquid to be dosed.
  • Both liquids are separated from each other by a seal sliding in the dosing "element".
  • the seal must be sufficiently soft so that it can be moved by the displacement medium.
  • the compression of the displacement medium is therefore partially absorbed by the seal, and errors occur during metering, especially in the volume range of a few microliters.
  • Dosing "elements” with very small diameters are also difficult to manufacture according to this OS. They also have the disadvantage that they have to be pre-filled with the compression medium before the dosing process begins and that the liquid to be dosed comes into contact with a surface which was previously wetted with the compression medium.
  • dosing vessel of which can be filled with the liquid to be dosed and then temporarily separated from the pipette without the dosing vessel emptying in an uncontrolled manner dosing could be done as precisely as with a capillary piston pipette, and at the same time without carryover as with a piston piston pipette of the DT -PS 12 91 142.
  • the requirements for the dimensional accuracy of the measuring device in The area of the connection to the dosing vessel and the shape accuracy of the entire dosing vessel should be small, the dosing vessels should be inexpensive to dispose of and they should be able to be connected to or detached from the measuring device quickly and without effort, and yet the connection should seal well .
  • the measuring device has one or more independent measuring rooms, any number of which are connected to a metering vessel.
  • a metering vessel (3a) which is sealed airtight at its upper end with a resilient body (6a) made of silicone rubber, is connected to a measuring device (1), which has a resilient body at its outlet piercing hollow needle (2).
  • the air contained in the metering vessel is sucked out with the measuring device, the liquid of a supply vessel flowing into the metering vessel.
  • the filled dosing vessel is separated from the measuring device after the pressure equalization, and according to the inventive idea, the liquid cannot now flow out of the dosing vessel.
  • the absorbed liquid is kept in the metering vessel and stored, or defined volumes of a displacement medium are injected into the interior of the metering vessel with the same or with a second measuring device, all at once or in several steps.
  • the same volumes of the liquid to be dosed flow out of the dosing vessel which is open at its lower end. If the volume to be dispensed does not have to be measured precisely, a precisely metering measuring device is not required to dispense the liquid: the interior of the metering vessel is temporarily subjected to an overpressure, or, if an air cushion is present, the surroundings are under a vacuum, which one is required can act for any length of time after the volume to be dispensed.
  • a used dosing vessel is exchanged for a fresh dosing vessel before taking up the new sample.
  • the discharge of the metering vessel is facilitated by a lever linkage.
  • air is used as the displacement medium. If high precision is desired when dispensing the liquid, a displacement medium that is as incompressible as possible in relation to air is used.
  • An essential advantage of the invention is that the air cushion causing the metering error can be removed by repositioning the metering vessel. It is therefore possible to precisely dose a few microliters or even fractions of microliters.
  • the invention also shows another way of filling the dosing vessel, in which an air cushion is avoided (FIG. 2): the liquid to be dosed is filled into the portion (3m0) of a still incompletely assembled or disassembled dosing vessel (3m1, 3m2) ) and then closes it by z. B. with one end (5), screwed, welded, or the parts soldered together, clamped or joined together by other means, resulting in a complete dosing vessel according to the invention.
  • Faults due to contamination of the liquid to be dosed with a liquid displacement medium are not to be expected if, for example, to displace aqueous solutions.
  • B. silicone oil is used. With water as a displacement medium for aqueous solutions, elongated thin dosing vessels are used, and both liquids are separated from each other by a small air bubble.
  • a metering vessel of the invention (FIG. 3) consists of a hollow vessel (3a, 3b) with two ends (4, 5), one (4) of which has a sealing resilient body (6a; e.g. silicone rubber) and the other (5) has a narrow opening.
  • the measuring device suitable for the metering vessel ' ' i has at its exit a sharp hollow needle (2) with which the resilient body (6a) is pierced. Then the liquid to be dosed is taken up and the dosing vessel is separated from the measuring device again. When the hollow needle is withdrawn, the puncture channel closes by itself.
  • the level of the liquid in the area of the opening rises by a certain short distance, thereby delaying drying out.
  • the mirror lowers again when the dosing vessel is connected to the measuring device again.
  • a diaphragm valve (6b), the diaphragm of which is raised by a vacuum created to absorb the liquid.
  • the membrane is pierced by the pointed hollow needle of a measuring device.
  • the resilient body (6a; e.g. silicone rubber) is held in position by rigid projections (7) of the wall of the metering vessel.
  • a piercing hollow needle can now only slightly deform or deflect the resilient body, and disruptions due to changes in the internal volume of the dosing vessels when piercing with the hollow needle are reduced.
  • a channel (8) is additionally provided in a body (9) of low deformability connected to the wall of the metering vessel.
  • This channel sealingly comprises the outlet (2; e.g. a needle) of the measuring device connected to the metering vessel. The inaccuracy of dosing caused by the compressibility of the elastic rubber seal is eliminated.
  • One embodiment has a membrane (10) which is permeable to air but impermeable to certain liquids, in the case of aqueous solutions, for.
  • a membrane (10) which is permeable to air but impermeable to certain liquids, in the case of aqueous solutions, for.
  • B. a water-repellent membrane which is sealingly attached between the projections (7) and the resilient body (6a). It prevents the liquid to be dosed from accidentally entering the measuring device.
  • the resilient body (6a) has an already preformed channel 11, which is closed as long as it is not connected to the outlet (2; e.g. with a blunt hollow needle) of the measuring device.
  • the channel is designed in such a way that it opens by finger pressure on the resilient body, as a result of which the interior is pressure-balanced with the surroundings and the previously filled liquid flows out of the metering vessel through its hydrostatic pressure to the other open or open end .
  • a dosing vessel (3i) provided with several outputs is suitable for the simultaneous dispensing of several partial samples.
  • Another metering vessel (3k) is designed such that all ends are first closed and have the same means as are provided for connecting the metering vessel to the measuring device in the sense of the invention.
  • One end is connected to the measuring device, the other end or the other ends are permanently or temporarily opened by plugging on bidirectional end pieces (13), these openings being so narrow that the liquid to be dosed, which is taken up into the dosing vessel, does not flow out as long as They in the area of these openings are not under one compared to the external environment the dosing vessel is at higher pressure.
  • the dosing vessels of the invention are not required to have a particularly high dimensional accuracy in most embodiments because the dosing vessels are not part of the metering device of the metering device which determines the dosing volume, such as the piston and the capillary of a capillary piston pipette, and because the seal between the metering device and the metering vessel is in most cases Cases is not based on the precisely matched fit of the output of the measuring device and the part of the metering vessel connected to this outlet, as in the device of DT-PS 12 91 142, but because the seal in most cases is connected to the metering vessel Measuring device only forms by itself, for. B. in all versions in which a resilient body (6a; z. B. a rubber seal) is pierced by a sharp hollow needle. Accordingly, these dosing vessels are also easy to manufacture and, furthermore, no great force is required to connect them to or remove them from a measuring device.
  • the invention enables a simultaneous examination of many small samples: the samples are first taken individually into the dosing vessels and then put in at their destination at the same time (with a measuring device having several independent measuring rooms) or very quickly one after the other (measuring device with only one measuring room) Secondary vessels. As a result, the dosing period between the first and the last sample is canceled or it is considerably reduced, and analysis errors caused by evaporation of the dosed samples of different lengths are avoided.
  • the entire sample is filled into a dosing vessel and the required tell-tale volumes are then dispensed into secondary vessels.
  • one test tube and several dosing vessels are saved for each sample, as well as the work involved in replacing the dosing vessels and filling the sample holder into the dosing vessels from the second dispensing step.
  • the filled dosing vessels of the invention are coded, taken from their original order and placed in a sequence in accordance with the requirements in a grid which is based on the "sample plate" of the measuring station. Then the partial volumes of the samples to be metered are output to the sample receiving points of the measuring station, one after the other or, if a measuring device having a plurality of independent measuring rooms is available, simultaneously. The dosing vessels are sorted back and are ready for distribution to other measuring stations.
  • At least one metering vessel is designed so that it has another end with a resilient hollow needle (2a) carrying its resilient body (6a) carrying end with which it is connected to another dosing vessel.
  • Several dosing vessels are connected to one another like a chain, an open connection being created for the interior of the dosing vessels, and the liquid to be dosed is filled (injected or sucked in) with the aid of a measuring device (syringe).
  • the capacity of the individual dosing vessels forming the chain and / or the penetration depth of the hollow needles into the dosing vessels are adapted to the intended, if necessary, different volumes of the partial samples.
  • the dosing vessels are made in the desired size immediately before filling.
  • This chain technique is also suitable for dividing a sample into several portions. If the portions are to be kept for a longer period of time, preference is given to using dosing vessels (3k) which are closed at all ends and carry at least two resilient bodies which can be pierced. They are joined together for filling with two-directional or multi-directional connecting pieces (13; e.g. hollow hollow needles at both ends).
  • the dosing vessel can also be used as a reaction vessel ( Figure 5).
  • reagents and samples are taken one after the other and reacted in the dosing vessel. This procedure saves pipette tips and test tubes.
  • the dosing vessel itself is placed in the beam path of a photometer, or its contents are previously filled into a measuring vessel.
  • the metering vessels of the invention can be connected to conventional measuring devices that work according to the most varied of principles.
EP19840115676 1984-12-18 1984-12-18 Appareil doseur pour liquides Expired EP0187167B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP19840115676 EP0187167B1 (fr) 1984-12-18 1984-12-18 Appareil doseur pour liquides
DE8484115676T DE3480383D1 (en) 1984-12-18 1984-12-18 Liquid dispenser

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP19840115676 EP0187167B1 (fr) 1984-12-18 1984-12-18 Appareil doseur pour liquides

Publications (2)

Publication Number Publication Date
EP0187167A1 true EP0187167A1 (fr) 1986-07-16
EP0187167B1 EP0187167B1 (fr) 1989-11-08

Family

ID=8192361

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19840115676 Expired EP0187167B1 (fr) 1984-12-18 1984-12-18 Appareil doseur pour liquides

Country Status (2)

Country Link
EP (1) EP0187167B1 (fr)
DE (1) DE3480383D1 (fr)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0597837A4 (fr) * 1990-04-11 1993-06-11 Commw Scient Ind Res Org Appareil et procede utilises pour l'elevage de nematodes, de champignons de cultures tissulaires et similaires et pour la recolte de nematodes.
WO1994011108A1 (fr) * 1992-11-11 1994-05-26 Labsystems Oy Receptacle
WO1997018895A2 (fr) * 1995-11-20 1997-05-29 Abion Beteiligungs- Und Verwaltungsgesellschaft Mbh Applicateur jetable et kit
WO1998015810A1 (fr) * 1996-10-07 1998-04-16 Saliva Diagnostic Systems, Inc. Procede de prelevement d'echantillons liquides a analyser
EP1093856A1 (fr) * 1999-10-21 2001-04-25 Tecan Schweiz AG Embout de pipette remplacable et procédé pour sa production, tête et système de pipettage associés
WO2002039916A1 (fr) * 2000-11-17 2002-05-23 Ilia Borisovitch Izvoztchikov Distributeur de milieu destine a contenir des echantillons histologiques et biologiques
US6634243B1 (en) 2002-01-14 2003-10-21 Rapid Medical Diagnostics Corporation Sample testing device
US7378054B2 (en) 2004-04-16 2008-05-27 Savvipharm Inc Specimen collecting, processing and analytical assembly
EP2050500A1 (fr) * 2007-10-19 2009-04-22 Lifescan Scotland Ltd Adaptateur de seringue
WO2010025826A2 (fr) * 2008-09-02 2010-03-11 Scienion Ag Organe de régulation de pression, en particulier clapet de ventilation pour ventiler un micro-doseur

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2611060A1 (de) * 1975-03-25 1976-10-14 Jean Marie Faure Geraet mit abgemessenen fluessigkeitsvolumina
US4016765A (en) * 1976-05-07 1977-04-12 Lee Tsao Piao Pipette controller with graduate reading plunger
EP0005979A1 (fr) * 1978-05-25 1979-12-12 Dynatech Ag Récipient de réaction miniature et méthode et appareil pour l'introduction d'un microvolume de liquide dans un tel récipient
US4189943A (en) * 1975-03-25 1980-02-26 Faure Jean M Apparatus for volume measurement of liquids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2611060A1 (de) * 1975-03-25 1976-10-14 Jean Marie Faure Geraet mit abgemessenen fluessigkeitsvolumina
US4189943A (en) * 1975-03-25 1980-02-26 Faure Jean M Apparatus for volume measurement of liquids
US4016765A (en) * 1976-05-07 1977-04-12 Lee Tsao Piao Pipette controller with graduate reading plunger
EP0005979A1 (fr) * 1978-05-25 1979-12-12 Dynatech Ag Récipient de réaction miniature et méthode et appareil pour l'introduction d'un microvolume de liquide dans un tel récipient

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RESEARCH DISCLOSURE, Nr. 202, Februar 1981, Seiten 79-84, Nr. 20218, Havant Hampshire, GB; "Procédé et dispositif pour le dosage de volumes liquides" *

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0597837A4 (fr) * 1990-04-11 1993-06-11 Commw Scient Ind Res Org Appareil et procede utilises pour l'elevage de nematodes, de champignons de cultures tissulaires et similaires et pour la recolte de nematodes.
EP0597837A1 (fr) * 1990-04-11 1994-05-25 Commw Scient Ind Res Org Appareil et procede utilises pour l'elevage de nematodes, de champignons de cultures tissulaires et similaires et pour la recolte de nematodes.
WO1994011108A1 (fr) * 1992-11-11 1994-05-26 Labsystems Oy Receptacle
AU676233B2 (en) * 1992-11-11 1997-03-06 Labsystems Oy Container
US5660797A (en) * 1992-11-11 1997-08-26 Labsystems Oy Apparatus and method of making a pipette receptacle
WO1997018895A2 (fr) * 1995-11-20 1997-05-29 Abion Beteiligungs- Und Verwaltungsgesellschaft Mbh Applicateur jetable et kit
WO1997018895A3 (fr) * 1995-11-20 1997-09-12 Abion Beteilungs Nud Verwaltun Applicateur jetable et kit
US5935864A (en) * 1996-10-07 1999-08-10 Saliva Diagnostic Systems Inc. Method and kit for collecting samples of liquid specimens for analytical testing
WO1998015810A1 (fr) * 1996-10-07 1998-04-16 Saliva Diagnostic Systems, Inc. Procede de prelevement d'echantillons liquides a analyser
EP1093856A1 (fr) * 1999-10-21 2001-04-25 Tecan Schweiz AG Embout de pipette remplacable et procédé pour sa production, tête et système de pipettage associés
US6431015B1 (en) 1999-10-21 2002-08-13 Tecan Trading Ag Delivery apparatus with interchangeable pipette tip
WO2002039916A1 (fr) * 2000-11-17 2002-05-23 Ilia Borisovitch Izvoztchikov Distributeur de milieu destine a contenir des echantillons histologiques et biologiques
US6634243B1 (en) 2002-01-14 2003-10-21 Rapid Medical Diagnostics Corporation Sample testing device
US7257991B2 (en) 2002-01-14 2007-08-21 Rapid Medical Diagnostics Corporation Sample testing device
US7378054B2 (en) 2004-04-16 2008-05-27 Savvipharm Inc Specimen collecting, processing and analytical assembly
EP2050500A1 (fr) * 2007-10-19 2009-04-22 Lifescan Scotland Ltd Adaptateur de seringue
WO2009052259A1 (fr) * 2007-10-19 2009-04-23 Lifescan Scotland, Ltd Adaptateur pour seringues
WO2010025826A2 (fr) * 2008-09-02 2010-03-11 Scienion Ag Organe de régulation de pression, en particulier clapet de ventilation pour ventiler un micro-doseur
WO2010025826A3 (fr) * 2008-09-02 2010-04-29 Scienion Ag Organe de régulation de pression, en particulier clapet de ventilation pour ventiler un micro-doseur

Also Published As

Publication number Publication date
EP0187167B1 (fr) 1989-11-08
DE3480383D1 (en) 1989-12-14

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