EP0176014B1 - Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser - Google Patents

Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser Download PDF

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Publication number
EP0176014B1
EP0176014B1 EP85111601A EP85111601A EP0176014B1 EP 0176014 B1 EP0176014 B1 EP 0176014B1 EP 85111601 A EP85111601 A EP 85111601A EP 85111601 A EP85111601 A EP 85111601A EP 0176014 B1 EP0176014 B1 EP 0176014B1
Authority
EP
European Patent Office
Prior art keywords
air
sample
liquid
mixing
jets
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP85111601A
Other languages
German (de)
English (en)
Other versions
EP0176014A3 (en
EP0176014A2 (fr
Inventor
Peter Scheffler
Wilfried Mauser
Thomas Grazianski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf Netheler Hinz GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Netheler Hinz GmbH filed Critical Eppendorf Netheler Hinz GmbH
Priority to AT85111601T priority Critical patent/ATE44888T1/de
Publication of EP0176014A2 publication Critical patent/EP0176014A2/fr
Publication of EP0176014A3 publication Critical patent/EP0176014A3/de
Application granted granted Critical
Publication of EP0176014B1 publication Critical patent/EP0176014B1/fr
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/40—Mixers using gas or liquid agitation, e.g. with air supply tubes
    • B01F33/407—Mixers using gas or liquid agitation, e.g. with air supply tubes by blowing gas on the material from above

Definitions

  • the invention relates to a method for mixing a liquid sample to be analyzed, in which the liquid sample in a sample container, in particular in a ku. vette is introduced and mixed by periodic air movement.
  • the invention further relates to a device for mixing a liquid sample to be analyzed contained in a sample container, in particular a cuvette, in which an air movement can be generated above the sample surface.
  • a method of the type mentioned at the outset is designed in such a way that air jets which shift the respective sample surface areas and generate turbulence in the liquid sample are directed alternately to different areas of the sample surface, the air jets preferably having a higher temperature than the sample liquid.
  • the movement and thus the mixing of the sample liquid takes place solely by alternately directing air jets onto different surface areas of the samples and thereby causing the sample liquid to move in a wave-like manner and thus to generate turbulence which results in the sample being mixed quickly .
  • the sample container which can be a standard vessel with a single liquid column, and / or essential parts of the device with which the air jets are directed onto the sample surface. be brought into contact with the sample container or the sample so that an effective and quick mixing of the liquid sample is achieved in a simple manner.
  • the evaporation of sample liquid when carrying out the mixing process can be further reduced by using air with an air humidity of approximately 100% for the air jets, so that this saturated air does not absorb any moisture from the sample liquid.
  • the object on which the invention is based is also achieved with a device of the type mentioned at the beginning by at least two air cannulas with their outlet openings above the sample container, which are connected to a pump device which alternately applies air pulses to them.
  • the mixing of a liquid sample to be analyzed can be carried out very easily, without the opening of the sample container having to be sealed and without there being any contact between parts of the device and the sample container and / or the liquid sample must come.
  • the air supplying the air cannulas can be conducted through a temperature chamber which can be heated in a controlled manner.
  • the air to be supplied to the air cannulas can be conducted through a humidification device, which can consist, for example, of a water bowl provided at the bottom of the temperature control chamber, through which the air is conducted.
  • a humidification device which can consist, for example, of a water bowl provided at the bottom of the temperature control chamber, through which the air is conducted.
  • the length and the clear cross section of the air cannulas can be chosen so that laminar air flows emerge from them.
  • the sample container can be covered with an air passage opening for the passage of the air jets, for example in the form of a film attached to the sample container or a resilient cover held on the device.
  • the device shown contains a temperature control chamber 3 and a pump 12 driven by a drive 20, which is connected to the temperature control chamber via connecting hoses 10 and 11.
  • the pump 12 has two pump chambers 18, 19, each of which is closed with a membrane 16, 17.
  • an actuating side 15 is fastened by means of screws, which has an incision into which an eccentric 14 extends.
  • This eccentric is seated on the shaft 13 driven by the drive 20 (not described in more detail) and is rotated in the direction of the arrow shown in FIG.
  • the connecting hoses 10 and 11 are each connected to one of the chambers 18, 19 and lead in the tempering chamber 3 in subchambers 6, 7, and at a short distance above their bottoms. Air cannulas 4, 5 extend downward through these floors from the temperature control chamber 3, while the upper ends of the air cannulas 4, 5 end relatively far above the connections for the connecting hoses 10 and 11 in the subchambers 6 and 7.
  • the temperature control chamber 3 is surrounded by an electric heating jacket 26, and in its wall there is a temperature sensor 27, by means of which the temperature control chamber 8 is selected by means of a schematically illustrated temperature controller 8 so that the desired temperature is maintained in the subchambers 6, 7 .
  • the air cannulas 4, 5 are led downwards out of the temperature control chamber 3 such that when a sample vessel 1 is positioned below the outlet openings of the air cannulas 4 and 5 they are in the area of the clear opening cross section of the sample container 1, but at the greatest possible distance from one another. while the sample vessel 1 is covered by a cover 22 which is resiliently held on the temperature control chamber 3 and has through openings 23 for the air jets.
  • the drive 20 drives the shaft 13 and thereby rotates the eccentric 14, as a result of which air is compressed periodically in the chambers 18 and 19 (FIG. 2) and thereby displaced from it and is pressed into the subchambers 6 and 7 via the connecting slots 10 and 11.
  • Water can be located at the bottom of these subchambers, so that the air supplied to the subchambers flows over this water and is humidified, while the air in the subchambers 6 and 7 is also heated to a temperature which is above the temperature of the sample to be mixed .
  • Air jets alternately emerge from the air cannulas 4 and 5, the frequency and duration of which depend on the structure and mode of operation of the pump 12. It should be mentioned that each time air is pressed out of one of the air cannulas, air is sucked back into the associated subchamber via the other air cannula and, via the connecting hose, into the associated chamber of the pump which is closed by a membrane.
  • the dimensions of the air cannulas 4 and 5 are preferably chosen so that laminar flows emerge from them.
  • air cannulas with a length of 40 mm and a clear inner diameter of 0.8 mm were used for an exit velocity of the air jets of 1 m / sec to 2 m / sec with a volume of 5 cm 3 / sec to IOCM3 / sec .
  • the laminar flow produced in this way resulted in effective mixing if the outlet ends of the air cannulas 4, 5 were 21 mm to 30 mm above the sample surface.
  • a sample container 1 for example a cuvette, containing a liquid sample 2 to be mixed is placed under the air cannulas 4 and 5, as is indicated in FIGS. 1 and 3.
  • an air jet indicated by an arrow in FIG. 1
  • this air jet in the laterally offset, 1 'designated sample container, which is identical to the sample container 1, causes a deformation of the surface of the liquid sample designated 2', as indicated in Figure 1.
  • the surface of the liquid sample is alternately deformed in connection with the turbulence indicated by curved arrows in the manner indicated in FIG. 1, which leads to leads to a quick and effective mixing of the liquid sample, whereby due to the elevated temperature and the high humidity of the air jets there is no fear of a change in temperature or loss of liquid in the liquid sample.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)

Claims (11)

1. Procédé de mélange d'un échantillon de liquide à analyser dans lequel l'échantillon de liquide est introduit dans un récipient à échantillon, en particulier dans une cuvette, et mélangé par déplacement périodique d'air, caractérisé en ce que des jets d'air sont dirigés sur des zones différentes de la surface de l'échantillon et déplacent alternativement les zones de surface de l'échantillon concerné, et provoquent des turbulences dans l'échantillon de liquide.
2. Procédé selon la revendication 1, caractérisé en ce qu'on utilise pour les jets d'air, de l'air chauffé à une température supérieure à la température du liquide de l'échantillon.
3. Procédé selon la revendication 1 ou 2, caractérisé en ce que l'on utilise pour les jets d'air de l'air à une humidité d'environ 100%.
4. Procédé selon l'une des revendications 1 à 3, caractérisé en ce que les jets d'air sont acheminés vers la surface de l'échantillon sous la forme d'écoulements laminaires.
5. Dispositif de mélange d'un échantillon de liquide à analyser, contenu dans un récipient à échantillon, en particulier dans une cuvette, dans lequel un déplacement d'air est produit au-dessus de la surface de l'échantillon, caractérisé par au moins deux canules à air (4, 5) disposées avec leurs orifices de sortie au-dessus du récipient à échantillon (1), ces canules étant reliées à un dispositif à pompe (12) leur envoyant alternativement de l'air pulsé.
6. Dispositif selon la revendication 5, caractérisé en ce que le dispositif à pompe (12) fonctionne à une fréquence comprise entre 9 Hz et 14 Hz.
7. Dispositif selon la revendication 5 ou 6, caractérisé en ce que l'air à envoyer aux canules à air (4, 5) est guidé à travers un compartiment de mise à température (3) à chauffage réglable.
8. Dispositif selon la revendication 7, caractérisé en ce que l'air à acheminer vers les canules à air (4, 5) est guidé à travers un dispositif d'humidification.
9. Dispositif selon la revendication 8, caractérisé en ce que le dispositif d'humidification est constitué d'une coupe à eau prévue sur le fond du compartiment de mise à température (3) au-dessus de laquelle est guidé l'air.
10. Dispositif selon l'une des revendications 5 à 9, caractérisé en ce que la longueur et la section transversale intérieure des canules à air (4, 5) sont choisies de manière à donner lieu à des courants d'air laminaires à la sortie de celles-ci.
11. Dispositif selon l'une des revendications 5 à 10, caractérisé par un couvercle (22) destiné aux récipients à échantillon (1) et présentant des orifices de passage d'air.
EP85111601A 1984-09-22 1985-09-13 Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser Expired EP0176014B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT85111601T ATE44888T1 (de) 1984-09-22 1985-09-13 Verfahren und vorrichtung zum mischen einer zu analysierenden fluessigkeitsprobe.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3434931 1984-09-22
DE19843434931 DE3434931A1 (de) 1984-09-22 1984-09-22 Verfahren und vorrichtung zum mischen einer zu analysierenden fluessigkeitsprobe

Publications (3)

Publication Number Publication Date
EP0176014A2 EP0176014A2 (fr) 1986-04-02
EP0176014A3 EP0176014A3 (en) 1987-04-29
EP0176014B1 true EP0176014B1 (fr) 1989-07-26

Family

ID=6246151

Family Applications (1)

Application Number Title Priority Date Filing Date
EP85111601A Expired EP0176014B1 (fr) 1984-09-22 1985-09-13 Procédé et dispositif pour mélanger un échantillon d'un liquide à analyser

Country Status (5)

Country Link
US (1) US4664526A (fr)
EP (1) EP0176014B1 (fr)
JP (1) JPS6180026A (fr)
AT (1) ATE44888T1 (fr)
DE (2) DE3434931A1 (fr)

Families Citing this family (13)

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Publication number Priority date Publication date Assignee Title
DE3707368A1 (de) * 1987-03-07 1988-09-15 Hoechst Ag Vorrichtung zum thermostatisieren und mischen des inhaltes von gefaessen einer mikrotitrationsplatte
WO1990013956A1 (fr) * 1989-04-28 1990-11-15 First Pacific Networks, Inc. Reseau informatique decentralise a multiplexage temporel et en frequence
US5595707A (en) * 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
DE4232096A1 (de) * 1992-09-25 1994-03-31 Boehringer Mannheim Gmbh Verfahren und Vorrichtung zum berührungslosen automatischen Mischen eines Reaktionsgemisches in einem Analysegerät
DE4306085A1 (de) * 1993-02-26 1994-09-01 Max Planck Gesellschaft Verfahren und Vorrichtung zum Durchmischen von Flüssigkeiten in Mikrotiterplatten
US6565533B1 (en) 2000-01-21 2003-05-20 Novus International, Inc. Inoculation apparatus and method
US8323984B2 (en) * 2002-12-19 2012-12-04 Beckman Coulter, Inc. Method and apparatus for mixing blood samples for cell analysis
US7648678B2 (en) 2002-12-20 2010-01-19 Dako Denmark A/S Method and system for pretreatment of tissue slides
DE102004028303A1 (de) * 2004-06-11 2005-12-29 Roche Diagnostics Gmbh Verfahren und Vorrichtung zum Nachweis von Analyten
DE102005034175A1 (de) * 2005-07-21 2007-01-25 Roche Diagnostics Gmbh Verfahren und Vorrichtung zur Erzeugung eines Feuchtluftstromes mit definierter relativer Feuchte
EP2838650B1 (fr) 2012-04-18 2019-09-25 Life Technologies Corporation Procédés et appareil pour transfert de masse entre un flux de gaz et un liquide
DE102012023900A1 (de) 2012-12-07 2014-06-12 Meiko Maschinenbau Gmbh & Co. Kg Förderaggregat
US9298397B2 (en) 2013-11-12 2016-03-29 Globalfoundries Inc. Nonvolatile storage thresholding for ultra-SSD, SSD, and HDD drive intermix

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2171399A (en) * 1934-10-24 1939-08-29 Louis Peters Method and apparatus for homogenizing powdered mixtures
US2193002A (en) * 1939-09-07 1940-03-12 Ish-Shalom Samu-El Convertible mixing apparatus
US3087840A (en) * 1958-06-16 1963-04-30 Macrosonic Process Corp Methods and means for producing physical, chemical and physicochemical effects by large-amplitude sound waves
US3398935A (en) * 1964-03-25 1968-08-27 Bausch & Lomb Mixing means
CH478410A (de) * 1966-10-11 1969-09-15 Greiner Electronic Ag Verfahren zur Bestimmung der Blutgerinnung
JPS4820078U (fr) * 1971-07-15 1973-03-07
BE788877A (fr) * 1971-09-17 1973-01-02 Vickers Ltd Agitation d'echantillons liquides pour l'obtention de melanges homogenes
JPS58137758A (ja) * 1982-02-10 1983-08-16 Omron Tateisi Electronics Co 血液撹拌装置
ATE23280T1 (de) * 1982-07-01 1986-11-15 Eppendorf Geraetebau Netheler Verfahren zur mischung von zu analysierenden fluessigkeitsproben.
WO1985003571A1 (fr) * 1984-02-01 1985-08-15 Hulette William C Systemes et procedes d'analyse clinique

Also Published As

Publication number Publication date
EP0176014A3 (en) 1987-04-29
ATE44888T1 (de) 1989-08-15
JPH0578779B2 (fr) 1993-10-29
EP0176014A2 (fr) 1986-04-02
US4664526A (en) 1987-05-12
DE3571768D1 (en) 1989-08-31
DE3434931A1 (de) 1986-03-27
DE3434931C2 (fr) 1987-10-08
JPS6180026A (ja) 1986-04-23

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