EP0032544B1 - Rotor à angle fixe pour ultracentrifugeuse - Google Patents

Rotor à angle fixe pour ultracentrifugeuse Download PDF

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Publication number
EP0032544B1
EP0032544B1 EP80107484A EP80107484A EP0032544B1 EP 0032544 B1 EP0032544 B1 EP 0032544B1 EP 80107484 A EP80107484 A EP 80107484A EP 80107484 A EP80107484 A EP 80107484A EP 0032544 B1 EP0032544 B1 EP 0032544B1
Authority
EP
European Patent Office
Prior art keywords
sample tube
abutment
tube
fixed
angle rotor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP80107484A
Other languages
German (de)
English (en)
Other versions
EP0032544A1 (fr
Inventor
Peter Fanger
Rolf Näff
Theodor Schmidhauser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cessione kontron Instruments Holding Nv
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of EP0032544A1 publication Critical patent/EP0032544A1/fr
Application granted granted Critical
Publication of EP0032544B1 publication Critical patent/EP0032544B1/fr
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes

Definitions

  • the invention relates to a fixed-angle rotor for ultracentrifuges with sample tubes and closure devices for tightly closing the sample tubes during centrifugation.
  • Commercial caps for sample tubes used in fixed-angle rotors of ultracentrifuges generally consist of an insertion part for insertion into the opening of the sample tube and a clamping device with which the edge of the sample tube is clamped in the closure device and in this way a firm and tight connection between the sample tube and the closure device.
  • the insertion part is cylindrical in its lower area, which is intended for insertion into the opening of the sample tube, with an outer diameter corresponding to the inner diameter of the tube. At the top, the cylindrical part has a thinner shoulder, which is threaded.
  • the clamping device usually contains a cover which has a conical surface which is inclined outwards or inwards and which is screwed to the plug-in part using a nut which matches the thread.
  • the conical surface presses the edge of the sample tube either inwards against a corresponding conical surface at the upper edge of the cylindrical area of the plug-in part or with the help of an O-ring seal outwards against a corresponding cylindrical extension of the lid (cf. e.g. Prospect of the Kontron company "Rotors, tubes accessories", pp. 26-27, published in 1979).
  • the aim of the present invention was a simplified closure device which does not have the disadvantages of the known closure devices and nevertheless offers the same safety as is necessary in conventional devices of this type for use in ultracentrifuges.
  • closure devices have a lower cylindrical part intended for insertion into the upper region of the sample tube with a diameter corresponding to the opening of the tube and an upper part located outside the tube with a diameter widened compared to the lower part and in that each rotor chamber has an abutment surrounding the tube, against which the closure device presses the upper edge region of the tube due to the centrifugal force.
  • the abutment preferably has a conical or flat ring surface which is concentric with the axis of the sample tube.
  • the abutment can consist of a ring that moves from below through the sample tube to the sliding ring.
  • the abutment can be formed in the rotor itself.
  • the sample tubes usually made of plastic can either have an opening edge without additional reinforcement, or can be provided with a bead-like reinforcement or a type of flange. If no additional precautions are taken at the edge of the sample tube, the closure device must have an additional thickening in the upper region of the part to be inserted into the tube, by means of which the sample tube is expanded so that a conical surface corresponding to the abutment is created on its outer diameter.
  • the closure device 3 shown in perspective in FIG. 1 is formed by an insertion part which is attached to a sample tube 2 and cooperates with a clamping ring 4.
  • the closure device 3 has a cylindrical lower part 5, which is provided for insertion into the sample tube 2 and whose outside diameter corresponds to the inside diameter of the sample tube 2.
  • the likewise cylindrical upper part 6 of the plug-in part located outside the sample tube has a diameter which is larger than the inside diameter of the sample tube. Between the two areas there is a shoulder 7 on which the upper edge of the sample tube 2 is in contact.
  • the lower part 5 of the closure device 3 has an area 8, the outside diameter of which is somewhat larger than the inside diameter of the sample tube 2, but only so large that the area 8 can still be inserted into the same while the sample tube is widened. Between the thickened area 8 of the lower part 5 and a thinner area there is a transition zone in the form of an expansion 9 which has a conical shape.
  • the second part of the closure device consists of the ring 4, which surrounds the upper edge of the sample tube from the outside.
  • the inner contour of the ring 4 corresponds to the contour of the sample tube pushed onto the closure device 3, i. H.
  • the inner surface of the ring 4 is initially cylindrical with a diameter that corresponds to the outer diameter of the sample tube, then conical and then again cylindrical with an inner diameter that corresponds to the outer diameter of the upper part 6.
  • the exact diameters of the conical and cylindrical regions of the closure device 3 and of the ring 4 are matched to one another and to the wall thickness of the sample tube 2 in such a way that the upper edge of the sample tube is clamped in by the assembly of the closure device.
  • the sample tube sealed in this way is inserted into a corresponding chamber of a fixed-angle rotor.
  • the rotor chamber is designed in such a way that it precisely receives the sample tube 2 with its lower part and, where the lower edge of the ring 4 is located when the sample tube is inserted, has a shoulder that supports the ring 4.
  • the conical region of the inner wall of the ring 4 forms an abutment during centrifugation, against which the closure device 3, with its conical region 9, presses the corresponding wall zone of the sample tube 2 as a result of the centrifugal force.
  • the connection between the closure device and the sample tube becomes better and better as the centrifuge speed increases as a result of the increasing centrifugal force.
  • the sample tube 2 has a circumferential thickening 10 with a conical lower surface at its upper edge. Since in this version the conical surface already exists on the sample tube, the closure device 3 can be made simpler by dispensing with the thickened area 8 and the conical transition area 9. However, the ring 4 in turn has a corresponding conical inner surface against which the circumferential bead 10 of the sample tube is pressed by the centrifugal force during centrifugation. In Figure 2a) it can also be seen how the ring 4 rests with its underside on the shoulder 11 of the rotor chamber.
  • Figure 2b shows a further simplified closure device. In its lower part, this again has the thickened region 8 and the conical transition region 9 of the version shown in FIG. 1. In this embodiment, however, a corresponding conical surface 12 of the wall of the rotor chamber takes the place of the ring. The conically widened area of the sample tube is pressed against this conical surface 12 by the centrifugal force.
  • FIG. 2 c shows an embodiment in which the sample tube has a circumferential flange 13 at its upper edge.
  • This flange which is flat in the embodiment shown in FIG. 2c), lies on a corresponding flat surface of the ring 4 and is pressed against this flat surface of the ring during centrifugation.
  • the ring 4 could of course also be dispensed with if the corresponding abutment surface is provided in the rotor chamber itself.
  • closure device For the closure device according to the invention, the same materials come into question as are used for conventional closure devices, that is to say preferably aluminum or titanium.
  • the closure device according to the invention is considerably simplified compared to the known closure devices. So it consists of only two parts, in some embodiments even only one part. Above all, handling is extremely simplified. Inserting and, if necessary, pushing on the ring are operations that can be carried out in the order of seconds. In contrast, the attachment of a closure device of the known type was a work step that required at least ten times the time.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)

Claims (7)

1. Rotor à angle fixe pour ultracentrifugeuse comportant des tubes à essai (2) qui sont disposés dans des chambres inclinées par rapport à l'axe du rotor, et des dispositifs de fermeture (3) formés par des pièces enfichables pour fermer de façon étanche les tubes à essai pendant la centrifugation, caractérisé en ce que les dispositifs de fermeture (3) possèdent une partie inférieure cylindrique (5) destinée à être introduite dans la zone supérieure du tube à essai et possédant un diamètre correspondant à l'ouverture du tube, et une partie supérieure (6), située à l'extérieur du tube et possédant un diamètre élargi par rapport à la partie inférieure, et que chaque chambre du rotor comporte une butée (4, 11, 12) entourant le tube et contre laquelle le dispositif de fermeture repousse la zone marginale supérieure du tube, sous l'effet de la force centrifuge.
2. Rotor à angle fixe selon la revendication 1, caractérisé en ce que la butée (4, 11, 12) possède une surface annulaire conique ou plane, concentrique à l'axe du récipient.
3. Rotor à angle fixe selon la revendication 2, caractérisé en ce que la partie (5) du dispositif de fermeture (3), qui s'étend dans l'espace intérieur du tube à essai, possède un élément de diamètre accru (élargissement (9)), au moyen duquel lors de l'enfichage, une zone périphérique du bord du tube à essai est élargie.
4. Rotor à angle fixe selon la revendication 3, caractérisé en ce que le contour de l'élargissement (9) correspond à celui de la butée.
5. Rotor à angle fixe selon la revendication 1, caractérisé en ce que la butée est réalisée dans un anneau (4) qui est glissé à partir du bas sur le tube à essai et dont la surface intérieure correspond au contour du tube à essai dans lequel est introduit le dispositif de fermeture.
6. Rotor à angle fixe selon la revendication 1, caractérisé en ce que la butée (11, 12) est ménagée dans la paroi intérieure de la chambre du rotor.
7. Rotor à angle fixe selon la revendication 3, caractérisé en ce que l'élargissement (9) est conique et que son inclinaison est égale à celle d'une butée conique correspondante (4, 12).
EP80107484A 1980-01-15 1980-11-29 Rotor à angle fixe pour ultracentrifugeuse Expired EP0032544B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH30580 1980-01-15
CH305/80 1980-01-15

Publications (2)

Publication Number Publication Date
EP0032544A1 EP0032544A1 (fr) 1981-07-29
EP0032544B1 true EP0032544B1 (fr) 1984-07-25

Family

ID=4183971

Family Applications (1)

Application Number Title Priority Date Filing Date
EP80107484A Expired EP0032544B1 (fr) 1980-01-15 1980-11-29 Rotor à angle fixe pour ultracentrifugeuse

Country Status (4)

Country Link
US (1) US4396381A (fr)
EP (1) EP0032544B1 (fr)
JP (2) JPS56105767A (fr)
DE (1) DE3068734D1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2118155B (en) * 1982-04-15 1985-11-20 Fisons Plc Centrifuge tube sealing assembly
JPS59138463U (ja) * 1983-03-04 1984-09-14 日立工機株式会社 遠心管用圧着蓋
US4552278A (en) * 1984-10-30 1985-11-12 E. I. Du Pont De Nemours And Company Crimpable capping assembly for a centrifuge tube
US4778074A (en) * 1987-07-08 1988-10-18 Honeywell Inc. Composite material and metal canister
US4830209A (en) * 1988-05-09 1989-05-16 Multi-Technology Inc. Fail safe releasible locks for cappped disposable centrifuge containers
US4953741A (en) * 1988-05-09 1990-09-04 Multi-Technology Inc. Medical fail safe releasible locks and/or seals for capped disposable centrifuge containers, cryogenic vials and the like
US4874102A (en) * 1988-05-09 1989-10-17 Multi-Technology Inc. Medical fail safe releasible locks and/or seals for capped disposable centrifuge containers, cryogenic vials and the like
US4896780A (en) * 1988-05-09 1990-01-30 Multi-Technology Inc. Fail safe releasible locks for capped disposable centrifuge containers
US4956103A (en) * 1988-05-09 1990-09-11 Multi-Technology Inc. Fail safe releasible locks for capped disposable centrifuge containers
US4902270A (en) * 1988-10-03 1990-02-20 Nalge Company Centrifuge tube
US7947236B2 (en) 1999-12-03 2011-05-24 Becton, Dickinson And Company Device for separating components of a fluid sample
JP4761254B2 (ja) * 2005-06-09 2011-08-31 日立工機株式会社 遠心機用の密封機構付試料容器およびそれを用いた遠心機
EP2326422B1 (fr) 2008-07-21 2013-07-17 Becton, Dickinson and Company Dispositif de séparation de phases par densité
MX2011000794A (es) 2008-07-21 2011-02-24 Becton Dickinson Co Dispositivo de separacion de fase por densidad.
MX2011000798A (es) 2008-07-21 2011-03-01 Becton Dickinson Co Dispositivo de separacion de fase por densidad.
WO2010078417A2 (fr) * 2008-12-31 2010-07-08 Tapones Escobar, S.A. Bouchon synthétique composite mettant en oeuvre un composant de verrouillage mutuel et procédé de fabrication
EP4119228A1 (fr) 2009-05-15 2023-01-18 Becton, Dickinson and Company Dispositif de séparation de phases de densité
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid
CN105562221B (zh) * 2016-03-07 2018-05-15 中国工程物理研究院总体工程研究所 振动离心机上的大质量试验件安装装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2627133A1 (de) * 1975-07-16 1977-02-10 Du Pont Dichtegradient-zentrifugation und zentrifuge
DE2756225A1 (de) * 1976-12-17 1978-06-22 Du Pont Zentrifugenrotor zum zentrifugieren einer probe in einem elastischen probenbehaelter
US4166573A (en) * 1977-12-01 1979-09-04 E. I. Du Pont De Nemours And Company Centrifuge tube enclosure
FR2439622A1 (fr) * 1978-10-28 1980-05-23 Fisons Ltd Dispositif de montage etanche d'un tube a echantillon sur le rotor d'une centrifugeuse

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3508653A (en) * 1967-11-17 1970-04-28 Charles M Coleman Method and apparatus for fluid handling and separation
US3800947A (en) * 1971-07-16 1974-04-02 P Smith Reagent tube and centrifugally operated solid-liquid separating device
US4190196A (en) * 1975-04-29 1980-02-26 E. I. Du Pont De Nemours And Company Centrifuge tube cap
US4087043A (en) * 1976-08-17 1978-05-02 Beckman Instruments, Inc. Dual seal arrangement for a centrifuge rotor tube cavity
US4102490A (en) * 1977-06-06 1978-07-25 Beckman Instruments, Inc. Data ring for vertical tube rotor
US4222513A (en) * 1978-12-12 1980-09-16 E. I. Du Pont De Nemours And Company Centrifuge tube seal

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2627133A1 (de) * 1975-07-16 1977-02-10 Du Pont Dichtegradient-zentrifugation und zentrifuge
DE2756225A1 (de) * 1976-12-17 1978-06-22 Du Pont Zentrifugenrotor zum zentrifugieren einer probe in einem elastischen probenbehaelter
US4166573A (en) * 1977-12-01 1979-09-04 E. I. Du Pont De Nemours And Company Centrifuge tube enclosure
FR2439622A1 (fr) * 1978-10-28 1980-05-23 Fisons Ltd Dispositif de montage etanche d'un tube a echantillon sur le rotor d'une centrifugeuse

Also Published As

Publication number Publication date
JPS58112452U (ja) 1983-08-01
DE3068734D1 (en) 1984-08-30
JPS56105767A (en) 1981-08-22
JPS613469Y2 (fr) 1986-02-03
US4396381A (en) 1983-08-02
EP0032544A1 (fr) 1981-07-29

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