EA201892663A1 - Аналоги фосфолипидного эфира для идентификации и выделения циркулирующих опухолевых клеток - Google Patents

Аналоги фосфолипидного эфира для идентификации и выделения циркулирующих опухолевых клеток

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Publication number
EA201892663A1
EA201892663A1 EA201892663A EA201892663A EA201892663A1 EA 201892663 A1 EA201892663 A1 EA 201892663A1 EA 201892663 A EA201892663 A EA 201892663A EA 201892663 A EA201892663 A EA 201892663A EA 201892663 A1 EA201892663 A1 EA 201892663A1
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EA
Eurasian Patent Office
Prior art keywords
tumor cells
circulating tumor
analogues
isolation
identification
Prior art date
Application number
EA201892663A
Other languages
English (en)
Inventor
Джейми Вайхерт
Чором Пак
Анатолий Пинчук
Кевин Козак
Марк Лонжино
Original Assignee
Селлектар Байосайенсис, Инк.
Джейми Вайхерт
Чором Пак
Анатолий Пинчук
Кевин Козак
Марк Лонжино
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Селлектар Байосайенсис, Инк., Джейми Вайхерт, Чором Пак, Анатолий Пинчук, Кевин Козак, Марк Лонжино filed Critical Селлектар Байосайенсис, Инк.
Publication of EA201892663A1 publication Critical patent/EA201892663A1/ru

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/12—Measuring magnetic properties of articles or specimens of solids or fluids
    • G01R33/1269—Measuring magnetic properties of articles or specimens of solids or fluids of molecules labeled with magnetic beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488—Methods for deciding
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
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  • Hospice & Palliative Care (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к способу идентификации, выделения и обеспечения последующего анализа циркулирующих опухолевых клеток, включающему приведение в контакт образца крови или сыворотки крови, полученного от субъекта, с композицией, содержащей аналог фосфолипидного эфира, связанный с люминесцентной молекулой или магнитной частицей, и подвергание образца крови или сыворотки крови, полученного от субъекта, флуоресцентной микроскопии, проточной цитометрии или выделению в магнитном поле.
EA201892663A 2016-06-14 2017-06-14 Аналоги фосфолипидного эфира для идентификации и выделения циркулирующих опухолевых клеток EA201892663A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662349713P 2016-06-14 2016-06-14
PCT/US2017/037549 WO2017218702A1 (en) 2016-06-14 2017-06-14 Phospholipid ether analogs for the identification and isolation of circulating tumor cells

Publications (1)

Publication Number Publication Date
EA201892663A1 true EA201892663A1 (ru) 2019-05-31

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EA201892663A EA201892663A1 (ru) 2016-06-14 2017-06-14 Аналоги фосфолипидного эфира для идентификации и выделения циркулирующих опухолевых клеток

Country Status (16)

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US (1) US11467159B2 (ru)
EP (1) EP3469367B1 (ru)
JP (1) JP7136770B2 (ru)
KR (1) KR20190037226A (ru)
CN (1) CN109791152B (ru)
AU (1) AU2017286604B2 (ru)
BR (1) BR112018077197A2 (ru)
CA (1) CA3027497C (ru)
DK (1) DK3469367T3 (ru)
EA (1) EA201892663A1 (ru)
ES (1) ES2952867T3 (ru)
IL (1) IL263687B2 (ru)
MX (1) MX2018015710A (ru)
NZ (1) NZ749272A (ru)
SI (1) SI3469367T1 (ru)
WO (1) WO2017218702A1 (ru)

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CN109154601B (zh) 2016-05-19 2022-11-29 斯坦福大学托管董事会 用于流动中的自动单细胞细胞学分类的系统和方法
EP3338806A1 (en) * 2016-12-21 2018-06-27 Université de Namur Method for functionalising nanoparticles
KR20200140835A (ko) * 2018-04-10 2020-12-16 셀렉타 바이오사이언시스, 인코퍼레이티드 인지질-플라베글린 접합체 및 표적화된 암 치료를 위해 이를 사용하는 방법
US11815507B2 (en) 2018-08-15 2023-11-14 Deepcell, Inc. Systems and methods for particle analysis
US10611995B2 (en) 2018-08-15 2020-04-07 Deepcell, Inc. Systems and methods for particle analysis
US20230066517A1 (en) * 2019-09-12 2023-03-02 Cellectar Biosciences, Inc. Phospholipid Ether Conjugates as Cancer-Targeting Drug Vehicles
KR102400464B1 (ko) * 2020-07-28 2022-05-19 부산대학교 산학협력단 암 줄기세포 검출용 바이오 마커 조성물
US20230251262A1 (en) * 2022-02-09 2023-08-10 Universitaesklinikum Hamburg-Eppendorf Enrichment, detection and characterization of circulating tumor cells with susd2 and enpp1
CN115389766B (zh) * 2022-08-29 2023-09-22 深圳市瑞格生物科技有限公司 用于诊断神经母细胞瘤是否发生骨髓浸润的标志物及其应用

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BR9907852A (pt) 1998-02-12 2000-10-24 Immunivest Corp Processos para detectar e enumerar células raras e cancerosas em uma população celular mista, para diagnosticar câncer de estágio precoce em um paciente de teste, para determinar a probabilidade de recorrência de câncer em um paciente humano anteriormente tratado de câncer, para distinguir um carcinoma confinado ao órgão de um carcinoma com propriedades metastáticas, para acompanhar a situação de remissão em um paciente humano com câncer que passa pelo tratamento de terapia contra o câncer e para aumentar quantidades de células epiteliais circulantes em uma amostra de sangue, partìcula magnética revestida, composição, conjuntos de teste para avaliar uma amostra de paciente quanto a presença de células raras circulantes, quanto a presença de células de tumor circulantes, quanto a presença de células de câncer de mama circulantes, quanto a presença de células de câncer de próstata circulantes, quanto a presença de células de câncer de cólon circulantes, quanto a presença de células de câncer de bexiga circulantes e para monitorar um paciente quanto a recorrência de câncer, e, fração de sangue periférico enriquecido quanto a células neoplásticas circulantes
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BRPI0709397A2 (pt) * 2006-03-13 2011-07-05 Veridex Llc propagação de células primárias
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US20100286510A1 (en) * 2009-05-11 2010-11-11 Pinchuk Anatoly Use of fluorescent phospholipid ether compounds in biopsies
EP2446271A1 (en) * 2009-06-26 2012-05-02 Erasmus University Medical Center Rotterdam Identifying circulating tumor cells (ctcs) using cd146 in breast cancers patients
CN105209489B (zh) * 2013-03-05 2019-06-14 得克萨斯州大学系统董事会 针对间质和上皮-间质转化的循环肿瘤细胞的特异性检测工具
KR101922322B1 (ko) * 2014-07-22 2018-11-26 가부시키가이샤 간멘에키켄큐쇼 말초 순환 암세포의 검출 방법 및 검출 장치
US9925283B2 (en) * 2015-04-10 2018-03-27 Wisconsin Alumni Research Foundation Alkylphosphocholine analogs for multiple myeloma imaging and therapy

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Publication number Publication date
EP3469367B1 (en) 2023-08-02
CA3027497C (en) 2023-12-05
JP7136770B2 (ja) 2022-09-13
EP3469367A1 (en) 2019-04-17
KR20190037226A (ko) 2019-04-05
MX2018015710A (es) 2019-06-10
CA3027497A1 (en) 2017-12-21
SI3469367T1 (sl) 2023-10-30
DK3469367T3 (da) 2023-08-28
CN109791152B (zh) 2023-03-03
NZ749272A (en) 2023-01-27
BR112018077197A2 (pt) 2019-08-20
AU2017286604B2 (en) 2023-05-18
AU2017286604A1 (en) 2019-01-03
WO2017218702A1 (en) 2017-12-21
IL263687A (en) 2019-02-03
CN109791152A (zh) 2019-05-21
IL263687B2 (en) 2023-09-01
US20170356914A1 (en) 2017-12-14
JP2019528456A (ja) 2019-10-10
US11467159B2 (en) 2022-10-11
IL263687B1 (en) 2023-05-01
EP3469367A4 (en) 2020-02-19
ES2952867T3 (es) 2023-11-06

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