MX2017009485A - Detección y aislamiento de células fetales basadas en microfluidos para prueba prenatal no invasiva. - Google Patents

Detección y aislamiento de células fetales basadas en microfluidos para prueba prenatal no invasiva.

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Publication number
MX2017009485A
MX2017009485A MX2017009485A MX2017009485A MX2017009485A MX 2017009485 A MX2017009485 A MX 2017009485A MX 2017009485 A MX2017009485 A MX 2017009485A MX 2017009485 A MX2017009485 A MX 2017009485A MX 2017009485 A MX2017009485 A MX 2017009485A
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MX
Mexico
Prior art keywords
fetal cell
isolation
fetal
specific antigen
cells
Prior art date
Application number
MX2017009485A
Other languages
English (en)
Inventor
Chen Fanqing
Chakrabarty Tania
Original Assignee
Unimed Biotech (Shanghai) Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unimed Biotech (Shanghai) Co Ltd filed Critical Unimed Biotech (Shanghai) Co Ltd
Publication of MX2017009485A publication Critical patent/MX2017009485A/es

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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0641—Erythrocytes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2600/00—Oligonucleotides characterized by their use
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Abstract

La modalidades descrita en la presente proporcionan métodos para aislamiento de células fetales para diagnosis prenatal no invasiva, que comprende proporcionar una muestra de sangre materna; aplicar la muestra de sangre materna a un filtro integrado en un dispositivo microfluídico para enriquecer de esta manera las células sanguíneas nucleadas de la muestra de sangre materna; etiquetar las células sanguíneas nucleadas enriquecidas, dentro del dispositivo microfluídico, con una porción de enlace fluorescente o molécula de afinidad que se enlaza específicamente a un antígeno específico de células fetales o a un antígeno específico de células no fetales; y aislar la células fetales. Las modalidades descritas en la presente proporcionan dispositivos microfluídicos integrados para el aislamiento no invasivo de células fetales, que comprende un filtro; una porción de enlace o molécula de afinidad que se enlaza específicamente a un antígeno específico de células fetales o un antígeno específico de células no fetales; y una cámara visualizable por microscopía.
MX2017009485A 2015-01-23 2016-01-19 Detección y aislamiento de células fetales basadas en microfluidos para prueba prenatal no invasiva. MX2017009485A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562107261P 2015-01-23 2015-01-23
PCT/US2016/013865 WO2016118484A1 (en) 2015-01-23 2016-01-19 Microfluidics based fetal cell detection and isolation for non-invasive prenatal testing

Publications (1)

Publication Number Publication Date
MX2017009485A true MX2017009485A (es) 2017-11-15

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ID=56417638

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Application Number Title Priority Date Filing Date
MX2017009485A MX2017009485A (es) 2015-01-23 2016-01-19 Detección y aislamiento de células fetales basadas en microfluidos para prueba prenatal no invasiva.

Country Status (9)

Country Link
US (1) US20180015470A1 (es)
EP (1) EP3247789A4 (es)
JP (1) JP2018508194A (es)
KR (1) KR20170120105A (es)
CN (1) CN107206380A (es)
AU (1) AU2016209521A1 (es)
CA (1) CA2974373A1 (es)
MX (1) MX2017009485A (es)
WO (1) WO2016118484A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018052730A1 (en) * 2016-09-13 2018-03-22 Unimed Biotech (Shanghai) Co., Ltd. Methods and devices for imaging objects on a microfluidic chip
WO2018191194A1 (en) * 2017-04-14 2018-10-18 Rarecyte, Inc. Enrichment and identification of fetal material
CN108795685B (zh) * 2017-04-28 2022-01-11 中国科学院苏州纳米技术与纳米仿生研究所 微流控芯片、其制法及胎儿有核红细胞捕获与释放方法
US11666915B2 (en) * 2017-10-19 2023-06-06 Tl Genomics Inc. Cell classification chip
EP3704058A4 (en) * 2017-10-31 2021-11-10 Astrego Diagnostics AB MICROFLUIDIC DEVICE FOR CELLULAR CHARACTERIZATION
US20230258663A9 (en) * 2019-05-02 2023-08-17 Kellbenx Incorporated Filtration-based methods for preparing fetal nucleated red blood cells (nrbcs) for diagnostic testing
CN111440696B (zh) * 2020-02-26 2023-02-24 德运康明(厦门)生物科技有限公司 胎儿细胞捕获模块、用于胎儿细胞捕获的微流控芯片，及它们的使用方法
CN112522194A (zh) * 2020-11-26 2021-03-19 北京贝康医学检验所有限公司 一种胎儿有核红细胞的捕获方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641628A (en) * 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
AUPR749901A0 (en) * 2001-09-06 2001-09-27 Monash University Method of identifying chromosomal abnormalities and prenatal diagnosis
US7166443B2 (en) * 2001-10-11 2007-01-23 Aviva Biosciences Corporation Methods, compositions, and automated systems for separating rare cells from fluid samples
CA2529285A1 (en) * 2003-06-13 2004-12-29 The General Hospital Corporation Microfluidic systems for size based removal of red blood cells and platelets from blood
US7469557B2 (en) * 2004-06-12 2008-12-30 Teresanne Griffin Method for forming a capillary column for filtering, separation and concentration
US20070196820A1 (en) * 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US20070059716A1 (en) * 2005-09-15 2007-03-15 Ulysses Balis Methods for detecting fetal abnormality
US20070059774A1 (en) * 2005-09-15 2007-03-15 Michael Grisham Kits for Prenatal Testing
AU2007260676A1 (en) * 2006-06-14 2007-12-21 Artemis Health, Inc. Rare cell analysis using sample splitting and DNA tags
US8137912B2 (en) * 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
ITTO20070307A1 (it) * 2007-05-04 2008-11-05 Silicon Biosystems Spa Metodo e dispositivo per la diagnosi prenatale non-invasiva
WO2010085815A1 (en) * 2009-01-26 2010-07-29 Artemis Health, Inc. Methods and compositions for identifying a fetal cell
WO2012016136A2 (en) * 2010-07-30 2012-02-02 The General Hospital Corporation Microscale and nanoscale structures for manipulating particles
JP6068354B2 (ja) * 2010-11-09 2017-01-25 アルセディ バイオテック アンパルトセルスカブＡＲＣＥＤＩ ＢＩＯＴＥＣＨ ＡｐＳ 母体血液中の胎児細胞の濃縮及び同定及びこれに使用するリガンド
KR20130061114A (ko) * 2011-11-30 2013-06-10 주식회사 맥스바이오텍 산전 트리플 마커 진단을 위한 랩 온어 칩과 이의 제조방법, 이를 위한 검사 키트
JP2016521134A (ja) * 2013-05-16 2016-07-21 バセトラ メディカル テクノロジー カンパニー リミテッド 母体血から捕捉する胎児細胞を使用する胎児診断

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JP2018508194A (ja) 2018-03-29
EP3247789A1 (en) 2017-11-29
US20180015470A1 (en) 2018-01-18
EP3247789A4 (en) 2018-09-12
WO2016118484A1 (en) 2016-07-28
AU2016209521A1 (en) 2017-08-17
CN107206380A (zh) 2017-09-26
CA2974373A1 (en) 2016-07-28
KR20170120105A (ko) 2017-10-30

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