EA201000836A1 - Улучшенные зонды для fret и их применение - Google Patents

Улучшенные зонды для fret и их применение

Info

Publication number
EA201000836A1
EA201000836A1 EA201000836A EA201000836A EA201000836A1 EA 201000836 A1 EA201000836 A1 EA 201000836A1 EA 201000836 A EA201000836 A EA 201000836A EA 201000836 A EA201000836 A EA 201000836A EA 201000836 A1 EA201000836 A1 EA 201000836A1
Authority
EA
Eurasian Patent Office
Prior art keywords
probe
reactive group
application
fret probes
reactive
Prior art date
Application number
EA201000836A
Other languages
English (en)
Inventor
Якобус Йоханнес Мария Ван Донген
Хосе Альберто Орфао Де Матос Коррейа Э Вале
Original Assignee
Эразмус Юниверсити Медикал Сентер Роттердам
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Эразмус Юниверсити Медикал Сентер Роттердам filed Critical Эразмус Юниверсити Медикал Сентер Роттердам
Publication of EA201000836A1 publication Critical patent/EA201000836A1/ru

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5748—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving oncogenic proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57496—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving intracellular compounds

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)

Abstract

Настоящее изобретение касается выявления, среди прочего, специфичных для опухолей слитых белков и межбелковых взаимодействий. Предоставляется набор, по меньшей мере, из первого и второго молекулярных зондов, в котором каждый зонд предоставляется с красителем, где названные красители вместе обеспечивают перенос энергии, в котором каждый зонд дополнительно предоставляется с реактивной группой, обеспечивающей близкое расположение названных, по меньшей мере, первого и второго зондов, где названная реактивная группа представляет собой олигонуклеотид, и где реактивная группа названного первого зонда не взаимодействует непосредственно с реактивной группой названного второго зонда.
EA201000836A 2007-11-21 2008-11-21 Улучшенные зонды для fret и их применение EA201000836A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US411207P 2007-11-21 2007-11-21
PCT/NL2008/050737 WO2009067009A1 (en) 2007-11-21 2008-11-21 Improved fret-probes and use thereof

Publications (1)

Publication Number Publication Date
EA201000836A1 true EA201000836A1 (ru) 2010-12-30

Family

ID=40317061

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201000836A EA201000836A1 (ru) 2007-11-21 2008-11-21 Улучшенные зонды для fret и их применение

Country Status (14)

Country Link
US (1) US20120003632A1 (ru)
EP (1) EP2210100B1 (ru)
JP (1) JP2011505549A (ru)
KR (1) KR101623992B1 (ru)
CN (1) CN101868728A (ru)
AT (1) ATE511649T1 (ru)
AU (1) AU2008326922B2 (ru)
BR (1) BRPI0819739A2 (ru)
CA (1) CA2706477C (ru)
DK (1) DK2210100T3 (ru)
EA (1) EA201000836A1 (ru)
ES (1) ES2367232T3 (ru)
WO (1) WO2009067009A1 (ru)
ZA (1) ZA201003394B (ru)

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DK3467500T3 (da) 2011-11-11 2021-08-16 Eli N Glezer Cobinder-understøttede assayfremgangsmåder
JPWO2014007369A1 (ja) * 2012-07-05 2016-06-02 国立研究開発法人国立がん研究センター Ｆｇｆｒ２融合遺伝子
CN103207166B (zh) * 2012-12-25 2015-06-03 西安交通大学 用于快速检测atp的荧光共振体系的制备方法
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
US10379741B2 (en) * 2014-04-17 2019-08-13 Seagate Technology Llc Dynamic storage device region provisioning
CN103937490B (zh) * 2014-04-24 2015-07-29 辽宁大学 一种检测线粒体中硫离子的荧光探针及其应用
JP6695280B2 (ja) 2014-05-15 2020-05-20 メソ スケール テクノロジーズ エルエルシー 改善されたアッセイ方法
RU2717956C2 (ru) * 2014-09-30 2020-03-27 Конинклейке Филипс Н.В. Способ обнаружения пространственной близости первого и второго эпитопов
CN106290988A (zh) * 2015-05-25 2017-01-04 中国科学院上海应用物理研究所 饱和共振能量转移超分辨探针及其制备方法和应用
EP3314011A1 (en) * 2015-06-26 2018-05-02 Albert-Ludwigs-Universität Freiburg Branched proximity hybridization assay
CN105136761A (zh) * 2015-09-06 2015-12-09 常州大学 一种检测量子点表面多肽数量的方法
US10591481B2 (en) * 2015-10-30 2020-03-17 Genentech, Inc. Methods of measuring factor D activity and potency of factor D inhibitors
EP3480320A4 (en) * 2016-06-30 2020-03-11 Biois Co., Ltd. BICATENARY NUCLEIC ACID SIGNAL PROBE AND TARGET MOLECULE DETECTION METHOD USING THE SAME
CN106554378B (zh) * 2016-10-26 2019-06-21 广西大学 两/三簇糖基罗丹明衍生物及其制备方法和应用
CN110121364A (zh) 2016-12-19 2019-08-13 文塔纳医疗系统公司 肽核酸缀合物
CN107353903B (zh) * 2017-06-28 2019-05-10 武汉大学 一种合成染料修饰dna功能化含镉量子点的方法及其应用
WO2019120635A1 (en) 2017-12-18 2019-06-27 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
US11604186B2 (en) * 2018-10-17 2023-03-14 Molecular Devices (Austria) GmbH Real time western blot assays utilizing fluorescence resonance energy transfer (FRET)

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US5714331A (en) * 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5804384A (en) * 1996-12-06 1998-09-08 Vysis, Inc. Devices and methods for detecting multiple analytes in samples
AU7716500A (en) * 1999-09-27 2001-04-30 Array Bioscience Corporation Particle structures with receptors for analyte detection
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
JP2001245699A (ja) * 2001-02-01 2001-09-11 Hitachi Ltd 核酸の検出方法
EP1382965B1 (en) * 2001-04-23 2005-11-30 Eiken Kagaku Kabushiki Kaisha Analysis method using reporter (label) intermolecular interaction
NZ540219A (en) * 2002-11-07 2008-08-29 Univ Erasmus Fret probes and methods for detecting interacting molecules
ES2352050T3 (es) * 2002-11-07 2011-03-18 Erasmus Universiteit Rotterdam Método y sondas para la detección de una proteína de fusión específica de un tumor.
EP1692486B1 (en) * 2003-12-12 2015-10-28 Saint Louis University Biosensors for detecting macromolecules and other analytes
JP4626350B2 (ja) * 2005-03-22 2011-02-09 ソニー株式会社 ハイブリダイゼーション検出に適する反応部などを有する流路系、該流路系を用いるハイブリダイゼーション検出装置
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes

Also Published As

Publication number Publication date
BRPI0819739A2 (pt) 2017-05-09
ZA201003394B (en) 2011-02-23
CN101868728A (zh) 2010-10-20
EP2210100A1 (en) 2010-07-28
ES2367232T3 (es) 2011-10-31
WO2009067009A1 (en) 2009-05-28
JP2011505549A (ja) 2011-02-24
KR20100105605A (ko) 2010-09-29
AU2008326922B2 (en) 2013-11-28
DK2210100T3 (da) 2011-09-05
EP2210100B1 (en) 2011-06-01
KR101623992B1 (ko) 2016-05-24
ATE511649T1 (de) 2011-06-15
US20120003632A1 (en) 2012-01-05
CA2706477A1 (en) 2009-05-28
AU2008326922A1 (en) 2009-05-28
CA2706477C (en) 2016-09-13

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