EA200600704A1 - Иммобилизация биокатализатора - Google Patents

Иммобилизация биокатализатора

Info

Publication number
EA200600704A1
EA200600704A1 EA200600704A EA200600704A EA200600704A1 EA 200600704 A1 EA200600704 A1 EA 200600704A1 EA 200600704 A EA200600704 A EA 200600704A EA 200600704 A EA200600704 A EA 200600704A EA 200600704 A1 EA200600704 A1 EA 200600704A1
Authority
EA
Eurasian Patent Office
Prior art keywords
preparing
aqueous solution
mixture
acrylic monomers
immobilization
Prior art date
Application number
EA200600704A
Other languages
English (en)
Other versions
EA015321B1 (ru
Inventor
Иоганнес Бартек
Карен Робенс
Яна Зигова
Original Assignee
Лонца Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Лонца Аг filed Critical Лонца Аг
Publication of EA200600704A1 publication Critical patent/EA200600704A1/ru
Publication of EA015321B1 publication Critical patent/EA015321B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/098—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer formed in the presence of the enzymes or microbial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Pyridine Compounds (AREA)

Abstract

Полиакриламидные гранулы, содержащие инкапсулированные клетки, получают способом, включающим следующие стадии: (1) получение водного раствора смеси акриловых мономеров, (2) получение суспензии клеток в водном растворе персульфата, (3) получение эмульсии водного раствора третичного амина в несмешивающейся с водой жидкости, которая необязательно содержит поверхностно-активное вещество (ПАВ), (4) смешивание раствора, полученного на стадии (1), и суспензии, полученной на стадии (2), (5) добавление смеси, полученной на стадии (4), в перемешиваемую эмульсию, полученную на стадии (3), и (6) полимеризация смеси акриловых мономеров и одновременное инкапсулирование клеток с образованием полиакриламидных гранул, содержащих инкапсулированные клетки.
EA200600704A 2003-10-27 2004-10-26 Способ получения полиакриламидных гранул, полиакриламидные гранулы и их применение EA015321B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03024648 2003-10-27
PCT/EP2004/012065 WO2005040373A1 (en) 2003-10-27 2004-10-26 Immobilization of biocatalyst

Publications (2)

Publication Number Publication Date
EA200600704A1 true EA200600704A1 (ru) 2006-10-27
EA015321B1 EA015321B1 (ru) 2011-06-30

Family

ID=34486107

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600704A EA015321B1 (ru) 2003-10-27 2004-10-26 Способ получения полиакриламидных гранул, полиакриламидные гранулы и их применение

Country Status (12)

Country Link
US (2) US20050089987A1 (ru)
EP (1) EP1682659A1 (ru)
JP (2) JP5107577B2 (ru)
KR (1) KR20060100396A (ru)
CN (1) CN1871345B (ru)
AU (1) AU2004284226B2 (ru)
BR (1) BRPI0415884A (ru)
CA (1) CA2540251A1 (ru)
EA (1) EA015321B1 (ru)
IL (1) IL174453A (ru)
MX (1) MXPA06004672A (ru)
WO (1) WO2005040373A1 (ru)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050266027A1 (en) * 2004-05-25 2005-12-01 Watson James B Live organism product
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
EP3256850A4 (en) 2015-02-09 2018-10-10 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
CN104894092A (zh) * 2015-05-13 2015-09-09 安徽国星生物化学有限公司 一种仿生固定化3-氰基吡啶腈水合酶的制备方法
WO2017055518A1 (en) * 2015-09-30 2017-04-06 Basf Se Means and methods for producing an amide compound
CN108660131B (zh) * 2018-04-27 2020-07-21 浙江工业大学 固定化腈基水合酶和(s)-n-乙基吡咯烷-2-甲酰胺的制备方法
CN115004009A (zh) 2020-01-24 2022-09-02 弹弓生物科学公司 用于细胞样校准颗粒的组合物和方法
EP4147049A1 (en) 2020-05-04 2023-03-15 Slingshot Biosciences, Inc. Compositions and methods for passive optical barcoding for multiplexed assays
CN113106130B (zh) * 2020-06-23 2023-03-14 核芯生物医药科技(杭州)有限公司 聚集性葡萄球菌来源的腈水合酶在制备酰胺吡嗪类化合物中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE441009B (sv) * 1982-03-08 1985-09-02 Kjell Nilsson Sett att immobilisera levande biomaterial i perlformiga polymerer
EP0160260A3 (de) * 1984-05-02 1986-10-08 Bayer Ag Verfahren zur Immobilisierung von biologischem Material
EP0839190A1 (en) * 1995-08-09 1998-05-06 Ciba Specialty Chemicals Water Treatments Limited Processes for the production of amidase
ZA968485B (en) * 1995-11-01 1997-05-20 Lonza Ag Process for preparing nicotinamide
GB9525372D0 (en) * 1995-12-12 1996-02-14 Allied Colloids Ltd Enzymes, their preparation and their use in the production of ammonium acrylate
WO1997029087A1 (en) * 1996-02-09 1997-08-14 Reilly Industries, Inc. Continuous processes for the hydrolysis of cyanopyridines under substantially adiabatic conditions
US5863750A (en) * 1996-12-18 1999-01-26 Cytec Tech Corp Methods for the detoxification of nitrile and/or amide compounds

Also Published As

Publication number Publication date
MX281018B (ru) 2010-11-16
MXPA06004672A (es) 2006-11-20
AU2004284226B2 (en) 2009-04-09
CN1871345A (zh) 2006-11-29
KR20060100396A (ko) 2006-09-20
JP2012196213A (ja) 2012-10-18
US20070259415A1 (en) 2007-11-08
JP5107577B2 (ja) 2012-12-26
JP2007508851A (ja) 2007-04-12
AU2004284226A1 (en) 2005-05-06
WO2005040373A1 (en) 2005-05-06
EA015321B1 (ru) 2011-06-30
CN1871345B (zh) 2014-05-07
CA2540251A1 (en) 2005-05-06
EP1682659A1 (en) 2006-07-26
BRPI0415884A (pt) 2007-01-09
IL174453A (en) 2010-11-30
US20050089987A1 (en) 2005-04-28
IL174453A0 (en) 2006-08-01

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU