EA037565B1 - Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка - Google Patents

Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка Download PDF

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Publication number
EA037565B1
EA037565B1 EA201691532A EA201691532A EA037565B1 EA 037565 B1 EA037565 B1 EA 037565B1 EA 201691532 A EA201691532 A EA 201691532A EA 201691532 A EA201691532 A EA 201691532A EA 037565 B1 EA037565 B1 EA 037565B1
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EA
Eurasian Patent Office
Prior art keywords
cell
cells
culture
cell culture
protein
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EA201691532A
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English (en)
Russian (ru)
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EA201691532A1 (ru
Inventor
Шивани Гупта
Сохие Канг
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Эмджен Инк.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53757626&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA037565(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Эмджен Инк. filed Critical Эмджен Инк.
Publication of EA201691532A1 publication Critical patent/EA201691532A1/ru
Publication of EA037565B1 publication Critical patent/EA037565B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01094—Protein N-acetylglucosaminyltransferase (2.4.1.94)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
EA201691532A 2014-01-29 2014-12-11 Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка EA037565B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461933137P 2014-01-29 2014-01-29
PCT/US2014/069744 WO2015116315A1 (en) 2014-01-29 2014-12-11 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Publications (2)

Publication Number Publication Date
EA201691532A1 EA201691532A1 (ru) 2017-01-30
EA037565B1 true EA037565B1 (ru) 2021-04-14

Family

ID=53757626

Family Applications (2)

Application Number Title Priority Date Filing Date
EA201691532A EA037565B1 (ru) 2014-01-29 2014-12-11 Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка
EA202190244A EA202190244A3 (ru) 2014-01-29 2014-12-11 Сверхэкспрессия регуляторов пути n-гликозилирования для модуляции гликозилирования рекомбинантных белков

Family Applications After (1)

Application Number Title Priority Date Filing Date
EA202190244A EA202190244A3 (ru) 2014-01-29 2014-12-11 Сверхэкспрессия регуляторов пути n-гликозилирования для модуляции гликозилирования рекомбинантных белков

Country Status (14)

Country Link
US (3) US10227627B2 (enExample)
EP (1) EP3099806A1 (enExample)
JP (4) JP6474420B2 (enExample)
KR (3) KR102301034B1 (enExample)
CN (2) CN106170554B (enExample)
AU (3) AU2014380174C1 (enExample)
BR (1) BR112016017606A2 (enExample)
CA (1) CA2938079C (enExample)
CL (1) CL2016001900A1 (enExample)
EA (2) EA037565B1 (enExample)
IL (3) IL282517B (enExample)
MX (2) MX377780B (enExample)
SG (1) SG10201912081RA (enExample)
WO (1) WO2015116315A1 (enExample)

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WO2020251537A1 (en) * 2019-06-10 2020-12-17 CHO Plus Inc. Cell lines for high level production of protein-based pharmaceuticals
KR101936049B1 (ko) 2015-10-15 2019-01-08 (주)알테오젠 IgG Fc 도메인을 가지는 융합 단백질의 생산방법
AU2018235928B2 (en) * 2017-03-14 2023-09-21 Amgen Inc. Control of total afucosylated glycoforms of antibodies produced in cell culture
IL307155B2 (en) 2018-05-01 2025-11-01 Amgen Inc Antibodies with modulated glycan profiles
CN109576212B (zh) * 2018-12-14 2021-06-22 杭州奕安济世生物药业有限公司 高密度接种培养中种子细胞的培养方法及其应用
US20240190951A1 (en) 2021-04-23 2024-06-13 Amgen Inc. Anti-tslp antibody compositions and uses thereof
MX2023012903A (es) * 2021-05-03 2023-11-08 Boehringer Ingelheim Int Metodo para producir spesolimab.
CN113684322B (zh) * 2021-09-09 2024-01-23 上海药明生物技术有限公司 一种降低抗体类蛋白高聚甘露糖型水平的方法
CN114525252B (zh) * 2022-03-10 2023-12-01 广州源井生物科技有限公司 一种提高mda-mb-231单细胞克隆形成率的单克隆增强培养基与培养方法及其应用
TW202426485A (zh) 2022-10-26 2024-07-01 美商安進公司 抗ｔｓｌｐ抗體組成物及其用途
WO2026130515A1 (en) * 2024-12-20 2026-06-25 Wuxi Biologics (Shanghai) Co., Ltd. Nucleic acid construct, vector, and approach to reduce high mannose of recombinant protein

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Also Published As

Publication number Publication date
CA2938079A1 (en) 2015-08-06
WO2015116315A1 (en) 2015-08-06
EA201691532A1 (ru) 2017-01-30
IL246914B (en) 2019-12-31
BR112016017606A2 (pt) 2017-10-10
JP2019088305A (ja) 2019-06-13
IL270861B (en) 2021-05-31
AU2020239681B2 (en) 2022-06-23
US20190153496A1 (en) 2019-05-23
AU2018264100B2 (en) 2020-07-23
AU2018264100A1 (en) 2018-12-06
EP3099806A1 (en) 2016-12-07
MX2020012356A (es) 2021-01-29
JP6797950B2 (ja) 2020-12-09
AU2020239681A1 (en) 2020-10-15
EA202190244A2 (ru) 2021-05-31
KR20210114549A (ko) 2021-09-23
US20190010532A1 (en) 2019-01-10
KR20220086703A (ko) 2022-06-23
JP7034236B2 (ja) 2022-03-11
US20200277642A1 (en) 2020-09-03
AU2014380174B2 (en) 2018-08-30
AU2014380174C1 (en) 2025-06-05
JP6474420B2 (ja) 2019-02-27
CN113774084A (zh) 2021-12-10
JP2021040638A (ja) 2021-03-18
CN106170554A (zh) 2016-11-30
KR20160111943A (ko) 2016-09-27
US10227627B2 (en) 2019-03-12
CA2938079C (en) 2022-10-11
US10655156B2 (en) 2020-05-19
IL246914A0 (en) 2016-09-29
AU2014380174A1 (en) 2016-08-11
CL2016001900A1 (es) 2017-05-12
CN106170554B (zh) 2021-08-17
IL282517A (en) 2021-06-30
KR102410393B1 (ko) 2022-06-16
MX377780B (es) 2025-03-11
US10907186B2 (en) 2021-02-02
JP2017505620A (ja) 2017-02-23
SG10201912081RA (en) 2020-02-27
IL270861A (en) 2020-01-30
MX2016009911A (es) 2016-10-28
KR102519540B1 (ko) 2023-04-10
JP2022068365A (ja) 2022-05-09
CN113774084B (zh) 2024-04-16
KR102301034B1 (ko) 2021-09-13
EA202190244A3 (ru) 2021-08-31
JP7788307B2 (ja) 2025-12-18
AU2018264100C1 (en) 2025-06-05
AU2020239681C1 (en) 2025-06-05
IL282517B (en) 2022-07-01

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