EA037565B1 - Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка - Google Patents

Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка Download PDF

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Publication number
EA037565B1
EA037565B1 EA201691532A EA201691532A EA037565B1 EA 037565 B1 EA037565 B1 EA 037565B1 EA 201691532 A EA201691532 A EA 201691532A EA 201691532 A EA201691532 A EA 201691532A EA 037565 B1 EA037565 B1 EA 037565B1
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Prior art keywords
cell
cells
culture
cell culture
protein
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EA201691532A
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English (en)
Russian (ru)
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EA201691532A1 (ru
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Шивани Гупта
Сохие Канг
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Эмджен Инк.
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53757626&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA037565(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Эмджен Инк. filed Critical Эмджен Инк.
Publication of EA201691532A1 publication Critical patent/EA201691532A1/ru
Publication of EA037565B1 publication Critical patent/EA037565B1/ru

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01101Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01094Protein N-acetylglucosaminyltransferase (2.4.1.94)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01143Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
EA201691532A 2014-01-29 2014-12-11 Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка EA037565B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461933137P 2014-01-29 2014-01-29
PCT/US2014/069744 WO2015116315A1 (en) 2014-01-29 2014-12-11 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Publications (2)

Publication Number Publication Date
EA201691532A1 EA201691532A1 (ru) 2017-01-30
EA037565B1 true EA037565B1 (ru) 2021-04-14

Family

ID=53757626

Family Applications (2)

Application Number Title Priority Date Filing Date
EA201691532A EA037565B1 (ru) 2014-01-29 2014-12-11 Способ снижения содержания высокоманнозных гликоформ рекомбинантного белка
EA202190244A EA202190244A3 (ru) 2014-01-29 2014-12-11 Сверхэкспрессия регуляторов пути n-гликозилирования для модуляции гликозилирования рекомбинантных белков

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EA202190244A EA202190244A3 (ru) 2014-01-29 2014-12-11 Сверхэкспрессия регуляторов пути n-гликозилирования для модуляции гликозилирования рекомбинантных белков

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US (3) US10227627B2 (enExample)
EP (1) EP3099806A1 (enExample)
JP (4) JP6474420B2 (enExample)
KR (3) KR102519540B1 (enExample)
CN (2) CN113774084B (enExample)
AU (3) AU2014380174C1 (enExample)
BR (1) BR112016017606A2 (enExample)
CA (1) CA2938079C (enExample)
CL (1) CL2016001900A1 (enExample)
EA (2) EA037565B1 (enExample)
IL (3) IL282517B (enExample)
MX (2) MX377780B (enExample)
SG (1) SG10201912081RA (enExample)
WO (1) WO2015116315A1 (enExample)

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KR101936049B1 (ko) 2015-10-15 2019-01-08 (주)알테오젠 IgG Fc 도메인을 가지는 융합 단백질의 생산방법
US12503700B2 (en) * 2017-03-14 2025-12-23 Amgen Inc. Control of total afucosylated glycoforms of antibodies produced in cell culture
IL321253A (en) 2018-05-01 2025-08-01 Amgen Inc Antibodies with a modulated glycan profile
CN109576212B (zh) * 2018-12-14 2021-06-22 杭州奕安济世生物药业有限公司 高密度接种培养中种子细胞的培养方法及其应用
BR112021024923A2 (pt) * 2019-06-10 2022-02-15 Cho Plus Inc Métodos de preparação de uma linha celular, de produção de um produto farmacêutico, de obtenção de uma linha celular e de produção de uma proteína, linhas celulares produtora e híbrida, sistema para produção de alto nível de produtos farmacêuticos, e, produto farmacêutico
AU2022262421A1 (en) 2021-04-23 2023-10-19 Amgen Inc. Anti-tslp antibody compositions and uses thereof
MX2023012903A (es) * 2021-05-03 2023-11-08 Boehringer Ingelheim Int Metodo para producir spesolimab.
CN113684322B (zh) * 2021-09-09 2024-01-23 上海药明生物技术有限公司 一种降低抗体类蛋白高聚甘露糖型水平的方法
CN114525252B (zh) * 2022-03-10 2023-12-01 广州源井生物科技有限公司 一种提高mda-mb-231单细胞克隆形成率的单克隆增强培养基与培养方法及其应用
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Also Published As

Publication number Publication date
EA202190244A3 (ru) 2021-08-31
CN106170554B (zh) 2021-08-17
EA202190244A2 (ru) 2021-05-31
AU2014380174C1 (en) 2025-06-05
KR20160111943A (ko) 2016-09-27
JP2022068365A (ja) 2022-05-09
JP2021040638A (ja) 2021-03-18
CN106170554A (zh) 2016-11-30
AU2018264100C1 (en) 2025-06-05
IL270861A (en) 2020-01-30
JP2019088305A (ja) 2019-06-13
JP6797950B2 (ja) 2020-12-09
KR102410393B1 (ko) 2022-06-16
JP2017505620A (ja) 2017-02-23
AU2014380174B2 (en) 2018-08-30
US10907186B2 (en) 2021-02-02
AU2018264100B2 (en) 2020-07-23
US20190010532A1 (en) 2019-01-10
AU2014380174A1 (en) 2016-08-11
AU2020239681C1 (en) 2025-06-05
WO2015116315A1 (en) 2015-08-06
EA201691532A1 (ru) 2017-01-30
US10227627B2 (en) 2019-03-12
BR112016017606A2 (pt) 2017-10-10
IL282517B (en) 2022-07-01
CN113774084A (zh) 2021-12-10
JP6474420B2 (ja) 2019-02-27
EP3099806A1 (en) 2016-12-07
CA2938079C (en) 2022-10-11
JP7788307B2 (ja) 2025-12-18
KR102301034B1 (ko) 2021-09-13
US10655156B2 (en) 2020-05-19
MX377780B (es) 2025-03-11
AU2020239681B2 (en) 2022-06-23
AU2018264100A1 (en) 2018-12-06
CN113774084B (zh) 2024-04-16
US20200277642A1 (en) 2020-09-03
JP7034236B2 (ja) 2022-03-11
IL246914B (en) 2019-12-31
IL282517A (en) 2021-06-30
KR20210114549A (ko) 2021-09-23
US20190153496A1 (en) 2019-05-23
KR20220086703A (ko) 2022-06-23
AU2020239681A1 (en) 2020-10-15
IL246914A0 (en) 2016-09-29
CL2016001900A1 (es) 2017-05-12
MX2020012356A (es) 2021-01-29
KR102519540B1 (ko) 2023-04-10
IL270861B (en) 2021-05-31
MX2016009911A (es) 2016-10-28
CA2938079A1 (en) 2015-08-06
SG10201912081RA (en) 2020-02-27

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