EA033008B1 - Экспрессионные векторы, содержащие химерные цитомегаловирусные промоторные и энхансерные последовательности - Google Patents

Экспрессионные векторы, содержащие химерные цитомегаловирусные промоторные и энхансерные последовательности

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Publication number
EA033008B1
EA033008B1 EA201590264A EA201590264A EA033008B1 EA 033008 B1 EA033008 B1 EA 033008B1 EA 201590264 A EA201590264 A EA 201590264A EA 201590264 A EA201590264 A EA 201590264A EA 033008 B1 EA033008 B1 EA 033008B1
Authority
EA
Eurasian Patent Office
Prior art keywords
sequence
regulatory
nucleic acid
chimeric
acid sequence
Prior art date
Application number
EA201590264A
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English (en)
Other versions
EA201590264A1 (ru
Inventor
Том Пейн
Роберт Янг
Марк Фиари
Original Assignee
ЛОНЗА БАЙОЛОДЖИС ПиЭлСи.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by ЛОНЗА БАЙОЛОДЖИС ПиЭлСи. filed Critical ЛОНЗА БАЙОЛОДЖИС ПиЭлСи.
Publication of EA201590264A1 publication Critical patent/EA201590264A1/ru
Publication of EA033008B1 publication Critical patent/EA033008B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Изобретение относится к экспрессионному вектору для гетерологичной экспрессии представляющей интерес последовательности нуклеиновой кислоты в клетках млекопитающих, содержащему химерную регуляторную последовательность, функционально связанную с подлежащей экспрессии гетерологичной последовательностью нуклеиновой кислоты, причем химерная регуляторная последовательность содержит (i) промоторную последовательность регуляторного элемента IE1 цитомегаловируса мыши или человека, которая функционально связана с сайтом начала транскрипции подлежащей экспрессии последовательности нуклеиновой кислоты; и (ii) энхансерную последовательность регуляторного элемента IE1 цитомегаловируса обезьяны, или химерную энхансерную последовательность регуляторных элементов IE1 цитомегаловируса человека и обезьяны, причем указанная энхансерная последовательность расположена на 5'-конце относительно указанной промоторной последовательности и функционально связана с ней. Изобретение также относится к клеткам-хозяевам млекопитающих, трансфицированным заявленным экспрессионным вектором, и способу гетерологичной экспрессии последовательности нуклеиновой кислоты посредством заявленного экспрессионного вектора.
EA201590264A 2012-09-24 2013-09-23 Экспрессионные векторы, содержащие химерные цитомегаловирусные промоторные и энхансерные последовательности EA033008B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12185728.8A EP2711426B1 (en) 2012-09-24 2012-09-24 Expression vectors comprising chimeric cytomegalovirus promoter and enhancer sequences
PCT/EP2013/069715 WO2014044845A1 (en) 2012-09-24 2013-09-23 Expression vectors comprising chimeric cytomegalovirus promoter and enhancer sequences

Publications (2)

Publication Number Publication Date
EA201590264A1 EA201590264A1 (ru) 2015-07-30
EA033008B1 true EA033008B1 (ru) 2019-08-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201590264A EA033008B1 (ru) 2012-09-24 2013-09-23 Экспрессионные векторы, содержащие химерные цитомегаловирусные промоторные и энхансерные последовательности

Country Status (20)

Country Link
US (3) US9777290B2 (ru)
EP (2) EP2711426B1 (ru)
JP (2) JP6162246B2 (ru)
KR (1) KR101757083B1 (ru)
CN (1) CN104718296B (ru)
AU (1) AU2013320157B2 (ru)
BR (1) BR112015005604B1 (ru)
CA (1) CA2880750C (ru)
DK (2) DK2711426T3 (ru)
EA (1) EA033008B1 (ru)
ES (2) ES2540753T3 (ru)
HK (1) HK1206061A1 (ru)
HU (2) HUE026516T2 (ru)
IL (1) IL237171B (ru)
MX (1) MX371007B (ru)
PL (2) PL2711426T3 (ru)
SG (1) SG11201502243YA (ru)
TR (1) TR201816314T4 (ru)
TW (1) TWI507526B (ru)
WO (1) WO2014044845A1 (ru)

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PL2711426T3 (pl) * 2012-09-24 2015-09-30 Lonza Biologics Plc Wektory ekspresyjne zawierające chimeryczne sekwencje promotora i wzmacniacza wirusa cytomegalii
US10106817B2 (en) * 2013-02-14 2018-10-23 The J. David Gladstone Institutes Compositions and methods of use thereof for identifying anti-viral agents
JP6566536B2 (ja) * 2014-06-16 2019-08-28 国立大学法人山口大学 フォワードプライマー
GB201603577D0 (en) * 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
CN109312362B (zh) * 2016-06-20 2022-06-28 扬森疫苗与预防公司 有效和平衡的双向启动子
CN110785494B (zh) 2017-06-16 2024-07-02 隆萨有限公司 用于生产生物制品的通用自调节哺乳动物细胞系平台
CN109988759A (zh) * 2017-12-29 2019-07-09 上海细胞治疗研究院 一种在t细胞中具有高转录活性的嵌合启动子
WO2020034097A1 (en) * 2018-08-14 2020-02-20 Wuxi Biologics (Shanghai) Co., Ltd. Transcriptional regulatory element and its use in enhancing the expression of exogenous protein
JP7483907B2 (ja) * 2020-02-19 2024-05-15 ウーシー バイオロジクス アイルランド リミテッド 強化された発現系及びその使用方法

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WO2004081167A2 (en) * 2003-03-11 2004-09-23 Applied Research Systems Ars Holding N.V. Expression vectors comprising the mcmv ie2 promoter
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WO2004081167A2 (en) * 2003-03-11 2004-09-23 Applied Research Systems Ars Holding N.V. Expression vectors comprising the mcmv ie2 promoter
WO2005035771A2 (en) * 2003-10-10 2005-04-21 Powderject Vaccines, Inc. Nucleic acid constructs
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Also Published As

Publication number Publication date
EP2898077A1 (en) 2015-07-29
SG11201502243YA (en) 2015-04-29
DK2711426T3 (en) 2015-07-13
EP2711426A1 (en) 2014-03-26
HUE026516T2 (en) 2016-06-28
IL237171A0 (en) 2015-04-30
JP6162246B2 (ja) 2017-07-12
ES2540753T3 (es) 2015-07-13
PL2898077T3 (pl) 2019-01-31
CN104718296A (zh) 2015-06-17
EP2898077B1 (en) 2018-08-08
CA2880750A1 (en) 2014-03-27
US10294491B2 (en) 2019-05-21
AU2013320157A1 (en) 2015-02-19
CA2880750C (en) 2019-01-08
US20200002721A1 (en) 2020-01-02
DK2898077T3 (en) 2018-12-03
HUE040463T2 (hu) 2019-03-28
WO2014044845A1 (en) 2014-03-27
KR20150042858A (ko) 2015-04-21
PL2711426T3 (pl) 2015-09-30
US20170356007A1 (en) 2017-12-14
US9777290B2 (en) 2017-10-03
JP2015532831A (ja) 2015-11-16
HK1206061A1 (en) 2015-12-31
US20150218584A1 (en) 2015-08-06
IL237171B (en) 2020-10-29
MX371007B (es) 2020-01-09
TR201816314T4 (tr) 2018-11-21
JP2017136081A (ja) 2017-08-10
MX2015002888A (es) 2015-10-14
KR101757083B1 (ko) 2017-07-11
CN104718296B (zh) 2018-04-10
TWI507526B (zh) 2015-11-11
TW201412983A (zh) 2014-04-01
EP2711426B1 (en) 2015-04-08
AU2013320157B2 (en) 2016-09-08
BR112015005604A2 (pt) 2017-08-08
BR112015005604B1 (pt) 2022-02-22
EA201590264A1 (ru) 2015-07-30
ES2694778T3 (es) 2018-12-27

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