MX2013008507A - Uso del promotor rd29 o fragmentos del mismo para expresion de transgenes inducible por estres en algodon. - Google Patents

Uso del promotor rd29 o fragmentos del mismo para expresion de transgenes inducible por estres en algodon.

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Publication number
MX2013008507A
MX2013008507A MX2013008507A MX2013008507A MX2013008507A MX 2013008507 A MX2013008507 A MX 2013008507A MX 2013008507 A MX2013008507 A MX 2013008507A MX 2013008507 A MX2013008507 A MX 2013008507A MX 2013008507 A MX2013008507 A MX 2013008507A
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MX
Mexico
Prior art keywords
nucleic acid
acid sequence
stress
cotton plant
cotton
Prior art date
Application number
MX2013008507A
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English (en)
Other versions
MX356630B (es
Inventor
Stephane Pien
Boer Bart Den
Original Assignee
Bayer Cropscience Nv
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Publication date
Application filed by Bayer Cropscience Nv filed Critical Bayer Cropscience Nv
Publication of MX2013008507A publication Critical patent/MX2013008507A/es
Publication of MX356630B publication Critical patent/MX356630B/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • C12N15/8238Externally regulated expression systems chemically inducible, e.g. tetracycline
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En un aspecto, la presente solicitud describe un gen quimérico que comprende (a) una primera secuencia de ácido nucleico que comprende por lo menos 400 nucleótidos consecutivos de SEQ ID NO: 1 o SEQ ID NO: 2 o una secuencia de ácido nucleico que tiene por lo menos 80% de identidad de secuencia al mismo cualquiera de las cuales confiere inducibilidad de estrés sobre dicho gen quimérico; (b) una segunda secuencia de ácido nucleico que codifica un producto de expresión de interés, que está implicado en la respuesta de una planta de algodón al estrés; y opcionalmente (c) una secuencia de terminación de transcripción y poliadenilación; en otro aspecto, la solicitud describe una célula de planta de algodón que comprende (a) un gen quimérico que comprende una primera secuencia de ácido nucleico que comprende por lo menos 400 nucleótidos consecutivos de SEQ ID NO: 1 o SEQ ID NO: 2 o una secuencia de ácido nucleico que tiene por lo menos 80 identidad de secuencia a la misma, cualquiera de las cuales confiere inducibilidad de estrés sobre dicho gen quimérico; (b) una segunda secuencia de ácido nucleico que codifica un producto de expresión de interés; y opcionalmente (c) una secuencia de terminación de transcripción y poliadenilación; además, la presente solicitud describe una planta de algodón, un método de expresión de un transgén en algodón bajo condiciones de estrés, un método para producir una planta de algodón, un método para detectar la expresión de un transgén bajo condiciones de estrés y un método para modular la resistencia de una planta de algodón al estrés como se caracteriza en las reivindicaciones.
MX2013008507A 2011-01-24 2012-01-24 Uso del promotor rd29 o fragmentos del mismo para expresión de transgenes inducible por estrés en algodón. MX356630B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161435495P 2011-01-24 2011-01-24
EP11075010 2011-01-24
EP11187147 2011-10-28
PCT/EP2012/051036 WO2012101118A1 (en) 2011-01-24 2012-01-24 Use of the rd29 promoter or fragments thereof for stress-inducible expression of transgenes in cotton

Publications (2)

Publication Number Publication Date
MX2013008507A true MX2013008507A (es) 2013-08-12
MX356630B MX356630B (es) 2018-06-06

Family

ID=45531407

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013008507A MX356630B (es) 2011-01-24 2012-01-24 Uso del promotor rd29 o fragmentos del mismo para expresión de transgenes inducible por estrés en algodón.

Country Status (8)

Country Link
US (2) US20140090102A1 (es)
EP (1) EP2668278A1 (es)
CN (1) CN103443279A (es)
AU (1) AU2012210618B2 (es)
BR (1) BR112013018531A2 (es)
MX (1) MX356630B (es)
WO (1) WO2012101118A1 (es)
ZA (1) ZA201304930B (es)

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EP2951298A1 (en) 2013-01-29 2015-12-09 The University Court of The University Of Glasgow Methods and means for increasing stress tolerance and biomass in plants
AU2014286296B2 (en) 2013-07-01 2020-04-09 Basf Se Methods and means for modulating flowering time in monocot plants
CA2957921A1 (en) 2014-07-22 2016-01-28 Nmc, Inc. Improved carbon fixation systems in plants and algae
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
CN107723301A (zh) * 2016-08-09 2018-02-23 新疆农业大学 一种棉花抗旱基因及其表达载体与应用
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
CN110819633A (zh) * 2018-08-09 2020-02-21 南京农业大学 一种胡萝卜ABA应答元件结合蛋白基因DcABF3的序列及其应用

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Also Published As

Publication number Publication date
US20140090102A1 (en) 2014-03-27
BR112013018531A2 (pt) 2016-10-18
AU2012210618B2 (en) 2017-02-02
MX356630B (es) 2018-06-06
CN103443279A (zh) 2013-12-11
ZA201304930B (en) 2014-09-25
AU2012210618A1 (en) 2013-08-01
WO2012101118A1 (en) 2012-08-02
EP2668278A1 (en) 2013-12-04
US20170260539A1 (en) 2017-09-14

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