EA000785B1 - Способ крупномасштабного выделения и очистки плазмидной днк - Google Patents

Способ крупномасштабного выделения и очистки плазмидной днк Download PDF

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Publication number
EA000785B1
EA000785B1 EA199700399A EA199700399A EA000785B1 EA 000785 B1 EA000785 B1 EA 000785B1 EA 199700399 A EA199700399 A EA 199700399A EA 199700399 A EA199700399 A EA 199700399A EA 000785 B1 EA000785 B1 EA 000785B1
Authority
EA
Eurasian Patent Office
Prior art keywords
plasmid dna
plasmid
dna
lysozyme
anion exchange
Prior art date
Application number
EA199700399A
Other languages
English (en)
Russian (ru)
Other versions
EA199700399A1 (ru
Inventor
Энн Л. Ли
Сэнгита Сагар
Original Assignee
Мерк Энд Ко., Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Мерк Энд Ко., Инк. filed Critical Мерк Энд Ко., Инк.
Publication of EA199700399A1 publication Critical patent/EA199700399A1/ru
Publication of EA000785B1 publication Critical patent/EA000785B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
EA199700399A 1995-05-19 1996-05-15 Способ крупномасштабного выделения и очистки плазмидной днк EA000785B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44611895A 1995-05-19 1995-05-19
PCT/US1996/007083 WO1996036706A1 (fr) 1995-05-19 1996-05-15 Procede de purification de plasmide effectuee a grande echelle

Publications (2)

Publication Number Publication Date
EA199700399A1 EA199700399A1 (ru) 1998-06-25
EA000785B1 true EA000785B1 (ru) 2000-04-24

Family

ID=23771380

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199700399A EA000785B1 (ru) 1995-05-19 1996-05-15 Способ крупномасштабного выделения и очистки плазмидной днк

Country Status (18)

Country Link
EP (1) EP0827536A1 (fr)
JP (1) JPH11505707A (fr)
KR (1) KR19990014924A (fr)
CN (1) CN1190435A (fr)
AR (1) AR003122A1 (fr)
AU (1) AU709003B2 (fr)
CA (1) CA2220867A1 (fr)
CZ (1) CZ366197A3 (fr)
EA (1) EA000785B1 (fr)
HR (1) HRP960222A2 (fr)
HU (1) HUP9802557A3 (fr)
MX (1) MX9708967A (fr)
NO (1) NO975280L (fr)
NZ (1) NZ309231A (fr)
PL (1) PL323475A1 (fr)
SK (1) SK155797A3 (fr)
WO (1) WO1996036706A1 (fr)
YU (1) YU29796A (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2807254C1 (ru) * 2022-12-26 2023-11-13 Федеральное государственное бюджетное учреждение науки "Федеральный исследовательский центр "Казанский научный центр Российской академии наук" Универсальный способ выделения ДНК и лизирующая смесь для его осуществления

Families Citing this family (32)

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US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
WO1996002658A1 (fr) * 1994-07-15 1996-02-01 Merck & Co., Inc. Procede de purification plasmidique a grande echelle
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2753204B1 (fr) * 1996-09-11 1998-12-04 Transgene Sa Procede de preparation d'adn plasmidique
SE9703532D0 (sv) 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
WO1999063076A1 (fr) * 1998-06-01 1999-12-09 The Immune Response Corporation Nouveau procede de purification plasmidique a grande echelle
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
EP1352059B1 (fr) * 2001-01-19 2005-11-09 Drm, Dr. Müller Ag Procede de traitement de biomasse pour la production de lysat cellulaire contenant de l'adn plasmidique
GB0107634D0 (en) 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
WO2006083721A2 (fr) 2005-01-31 2006-08-10 Merck & Co., Inc. Procede de purification amont et aval pour production a large echelle d'adn plasmidique
JP2006238822A (ja) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
DE102005059315A1 (de) * 2005-12-09 2007-06-14 Qiagen Gmbh Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren
CN100439497C (zh) * 2006-05-26 2008-12-03 吉林大学 一种适用于大规模生产质粒dna的纯化方法
WO2008045505A2 (fr) 2006-10-10 2008-04-17 Xenomics, Inc. Compositions, procédés et trousses pour isoler acides nucléiques à partir de fluides corporels au moyen d'un milieu d'échange anionique
KR101497728B1 (ko) * 2007-05-23 2015-03-03 이노비오 파마수티컬즈, 인크. 고농도의 생물학적 활성 분자 함유 조성물 및 그 제조 방법
CN101070543B (zh) * 2007-06-01 2010-12-29 广东药学院 超螺旋质粒分离纯化工艺
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
WO2011124709A1 (fr) 2010-04-08 2011-10-13 Qiagen Gmbh Dispositif chromatographique et procédé permettant l'isolement et la purification d'acides nucléiques
WO2011124705A1 (fr) * 2010-04-08 2011-10-13 Qiagen Gmbh Procédé d'isolement et de purification d'acides nucléiques
JP5624487B2 (ja) 2011-01-31 2014-11-12 横河電機株式会社 核酸抽出方法
WO2012146338A1 (fr) * 2011-04-27 2012-11-01 Merck Patent Gmbh Procédé pour la lyse de cellules
CN104099321A (zh) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 抗肺癌T-VISA-BikDD质粒的制备方法
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
CN112798697B (zh) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 慢病毒包装系统辅助质粒的超螺旋结构纯度的检测方法
CN114456940B (zh) * 2022-01-23 2024-05-03 和元生物技术（上海）股份有限公司 一种提高大肠杆菌裂解过程中质粒稳定性的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006309A1 (fr) * 1989-11-03 1991-05-16 Vanderbilt University Procede d'administration in vivo de genes etrangers fonctionnels
WO1993024640A2 (fr) * 1992-06-04 1993-12-09 The Regents Of The University Of California PROCEDES ET COMPOSITIONS UTILISES DANS UNE THERAPIE GENIQUE $i(IN VIVO)
WO1995007995A2 (fr) * 1993-09-13 1995-03-23 Applied Immune Sciences, Inc. Liposomes viraux adeno-associes et methodes associees
WO1996002658A1 (fr) * 1994-07-15 1996-02-01 Merck & Co., Inc. Procede de purification plasmidique a grande echelle

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006309A1 (fr) * 1989-11-03 1991-05-16 Vanderbilt University Procede d'administration in vivo de genes etrangers fonctionnels
WO1993024640A2 (fr) * 1992-06-04 1993-12-09 The Regents Of The University Of California PROCEDES ET COMPOSITIONS UTILISES DANS UNE THERAPIE GENIQUE $i(IN VIVO)
WO1995007995A2 (fr) * 1993-09-13 1995-03-23 Applied Immune Sciences, Inc. Liposomes viraux adeno-associes et methodes associees
WO1996002658A1 (fr) * 1994-07-15 1996-02-01 Merck & Co., Inc. Procede de purification plasmidique a grande echelle

Non-Patent Citations (4)

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Title
BIO-SCIENCES (1989), 8(1-2), 42-5 CODEN: BIOSER; ISSN: 0292-8418, XP000579530 COLOTE, S. ET AL: "Use of high-performance liquid chromatography in genetic engineering" *
BIOTECHNIQUES (1988), 6(9), 834, 837-8 CODEN: BTNQDO;ISSN: 0736-6205, XP000600195 LE BRUN, J. J. ET AL: "A simple method for the preparation of the covalently closed circular form of plasmid DNA" *
BIOTECHNIQUES (1992), 12(3), 430-4 CODEN: BTNQDO;ISSN: 0736-6205, XP002012269 HINES, RONALD N. ET AL: "Large-scale purification of plasmid DNA by anion-exchange high - performance liquid chromatography" see abstract *
SAMBROOK ET AL.: "Molecular cloning-1989. CSH LABORATORY PRESS, COLD SPRING HARBOR XP002011439 see page 1.34 - 1-35 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2807254C1 (ru) * 2022-12-26 2023-11-13 Федеральное государственное бюджетное учреждение науки "Федеральный исследовательский центр "Казанский научный центр Российской академии наук" Универсальный способ выделения ДНК и лизирующая смесь для его осуществления

Also Published As

Publication number Publication date
HUP9802557A3 (en) 2000-04-28
AU5921996A (en) 1996-11-29
NO975280D0 (no) 1997-11-18
AR003122A1 (es) 1998-07-08
WO1996036706A1 (fr) 1996-11-21
CA2220867A1 (fr) 1996-11-21
NO975280L (no) 1998-01-16
CZ366197A3 (cs) 1998-04-15
MX9708967A (es) 1998-03-31
PL323475A1 (en) 1998-03-30
HRP960222A2 (en) 1997-12-31
EA199700399A1 (ru) 1998-06-25
EP0827536A1 (fr) 1998-03-11
NZ309231A (en) 1999-10-28
AU709003B2 (en) 1999-08-19
SK155797A3 (en) 1998-07-08
KR19990014924A (ko) 1999-02-25
CN1190435A (zh) 1998-08-12
YU29796A (sh) 1999-06-15
HUP9802557A2 (hu) 1999-02-01
JPH11505707A (ja) 1999-05-25

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU