HUP9802557A3 - A method for large scale plasmid purification - Google Patents
A method for large scale plasmid purificationInfo
- Publication number
- HUP9802557A3 HUP9802557A3 HU9802557A HUP9802557A HUP9802557A3 HU P9802557 A3 HUP9802557 A3 HU P9802557A3 HU 9802557 A HU9802557 A HU 9802557A HU P9802557 A HUP9802557 A HU P9802557A HU P9802557 A3 HUP9802557 A3 HU P9802557A3
- Authority
- HU
- Hungary
- Prior art keywords
- large scale
- plasmid purification
- scale plasmid
- purification
- scale
- Prior art date
Links
- 238000000034 method Methods 0.000 title 1
- 239000013612 plasmid Substances 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US44611895A | 1995-05-19 | 1995-05-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUP9802557A2 HUP9802557A2 (en) | 1999-02-01 |
| HUP9802557A3 true HUP9802557A3 (en) | 2000-04-28 |
Family
ID=23771380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU9802557A HUP9802557A3 (en) | 1995-05-19 | 1996-05-15 | A method for large scale plasmid purification |
Country Status (18)
| Country | Link |
|---|---|
| EP (1) | EP0827536A1 (en) |
| JP (1) | JPH11505707A (en) |
| KR (1) | KR19990014924A (en) |
| CN (1) | CN1190435A (en) |
| AR (1) | AR003122A1 (en) |
| AU (1) | AU709003B2 (en) |
| CA (1) | CA2220867A1 (en) |
| CZ (1) | CZ366197A3 (en) |
| EA (1) | EA000785B1 (en) |
| HR (1) | HRP960222A2 (en) |
| HU (1) | HUP9802557A3 (en) |
| MX (1) | MX9708967A (en) |
| NO (1) | NO975280L (en) |
| NZ (1) | NZ309231A (en) |
| PL (1) | PL323475A1 (en) |
| SK (1) | SK155797A3 (en) |
| WO (1) | WO1996036706A1 (en) |
| YU (1) | YU29796A (en) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6197553B1 (en) | 1994-07-15 | 2001-03-06 | Merck & Co., Inc. | Method for large scale plasmid purification |
| CA2192342A1 (en) * | 1994-07-15 | 1996-02-01 | Ann L. Lee | A method for large scale plasmid purification |
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US5981735A (en) * | 1996-02-12 | 1999-11-09 | Cobra Therapeutics Limited | Method of plasmid DNA production and purification |
| FR2753204B1 (en) * | 1996-09-11 | 1998-12-04 | Transgene Sa | PROCESS FOR THE PREPARATION OF PLASMID DNA |
| SE9703532D0 (en) * | 1997-09-30 | 1997-09-30 | Pharmacia Biotech Ab | A process for the purification of plasmid DNA |
| US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| AU4314099A (en) * | 1998-06-01 | 1999-12-20 | Immune Response Corporation, The | Novel method of large scale plasmid purification |
| US6270970B1 (en) | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| US6310199B1 (en) | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| US20020012990A1 (en) | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| CA2446478C (en) | 2001-01-19 | 2008-02-26 | Drm, Dr. Muller Ag | Method for treating biomass for producing cell lysate containing plasmid dna |
| GB0107634D0 (en) | 2001-03-27 | 2001-05-16 | Fermentas Ab | Nucleic acid purification |
| US6406892B1 (en) | 2001-05-23 | 2002-06-18 | Bio-Rad Laboratories, Inc. | Acetate-free purification of plasmid DNA on hydroxyapatite |
| US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
| JP4971997B2 (en) | 2005-01-31 | 2012-07-11 | メルク・シャープ・エンド・ドーム・コーポレイション | Purification method of plasmid DNA |
| JP2006238822A (en) * | 2005-03-04 | 2006-09-14 | Fuji Photo Film Co Ltd | Method for separating and purifying nucleic acid |
| DE102005059315A1 (en) * | 2005-12-09 | 2007-06-14 | Qiagen Gmbh | Process for the enrichment of short-chain nucleic acids |
| CN100439497C (en) * | 2006-05-26 | 2008-12-03 | 吉林大学 | Purification method adapted to mass production of plasmid DNA |
| EP2081442B1 (en) * | 2006-10-10 | 2016-08-10 | TrovaGene, Inc. | Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media |
| AU2008256681B2 (en) * | 2007-05-23 | 2014-07-31 | Vgxi, Inc | Compositions comprising high concentration of biologically active molecules and processes for preparing the same |
| CN101070543B (en) * | 2007-06-01 | 2010-12-29 | 广东药学院 | Super spirial plasmid separation purifying process |
| GB0913160D0 (en) * | 2009-07-29 | 2009-09-02 | Avecia Biolog Ltd | Extraction process |
| EP2556158B1 (en) | 2010-04-08 | 2019-12-18 | Qiagen GmbH | Chromatographic device and method for isolating and purifying nucleic acids |
| JP2013523143A (en) * | 2010-04-08 | 2013-06-17 | キアゲン ゲーエムベーハー | Methods for isolating and purifying nucleic acids |
| JP5624487B2 (en) * | 2011-01-31 | 2014-11-12 | 横河電機株式会社 | Nucleic acid extraction method |
| US9493736B2 (en) | 2011-04-27 | 2016-11-15 | Merck Patent Gmbh | Method for lysing cells |
| CN104099321A (en) * | 2014-07-10 | 2014-10-15 | 广州市军科泰特医药科技有限公司 | Preparation method of lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD |
| CN106884011B (en) * | 2015-12-16 | 2020-11-17 | 固安鼎泰海规生物科技有限公司 | Combined liquid chromatography separation method for large-scale plasmid purification |
| CN111721871A (en) * | 2020-06-24 | 2020-09-29 | 南京济群生物科技有限公司 | High-resolution detection method for plasmid supercoiled DNA content |
| CN112798697B (en) * | 2020-11-16 | 2022-11-22 | 武汉波睿达生物科技有限公司 | Detection method for supercoiled structure purity of lentivirus packaging system helper plasmid |
| CN114456940B (en) * | 2022-01-23 | 2024-05-03 | 和元生物技术（上海）股份有限公司 | Method for improving plasmid stability in escherichia coli cracking process |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0452457T3 (en) * | 1989-11-03 | 1998-03-02 | Univ Vanderbilt | Method for in vivo removal of functional foreign genes |
| AU4528493A (en) * | 1992-06-04 | 1994-01-04 | Regents Of The University Of California, The | In vivo gene therapy with intron-free sequence of interest |
| US5834441A (en) * | 1993-09-13 | 1998-11-10 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Adeno-associated viral (AAV) liposomes and methods related thereto |
| CA2192342A1 (en) * | 1994-07-15 | 1996-02-01 | Ann L. Lee | A method for large scale plasmid purification |
-
1996
- 1996-05-09 AR ARP960102507A patent/AR003122A1/en unknown
- 1996-05-15 HU HU9802557A patent/HUP9802557A3/en unknown
- 1996-05-15 AU AU59219/96A patent/AU709003B2/en not_active Ceased
- 1996-05-15 MX MX9708967A patent/MX9708967A/en not_active IP Right Cessation
- 1996-05-15 EP EP96916486A patent/EP0827536A1/en not_active Withdrawn
- 1996-05-15 CA CA002220867A patent/CA2220867A1/en not_active Abandoned
- 1996-05-15 KR KR1019970708270A patent/KR19990014924A/en not_active Application Discontinuation
- 1996-05-15 CN CN96195329A patent/CN1190435A/en active Pending
- 1996-05-15 HR HR08/446,118A patent/HRP960222A2/en not_active Application Discontinuation
- 1996-05-15 PL PL96323475A patent/PL323475A1/en unknown
- 1996-05-15 JP JP8535061A patent/JPH11505707A/en not_active Withdrawn
- 1996-05-15 NZ NZ309231A patent/NZ309231A/en unknown
- 1996-05-15 CZ CZ973661A patent/CZ366197A3/en unknown
- 1996-05-15 SK SK1557-97A patent/SK155797A3/en unknown
- 1996-05-15 EA EA199700399A patent/EA000785B1/en not_active IP Right Cessation
- 1996-05-15 WO PCT/US1996/007083 patent/WO1996036706A1/en not_active Application Discontinuation
- 1996-05-17 YU YU29796A patent/YU29796A/en unknown
-
1997
- 1997-11-18 NO NO975280A patent/NO975280L/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2220867A1 (en) | 1996-11-21 |
| CZ366197A3 (en) | 1998-04-15 |
| AR003122A1 (en) | 1998-07-08 |
| NZ309231A (en) | 1999-10-28 |
| MX9708967A (en) | 1998-03-31 |
| KR19990014924A (en) | 1999-02-25 |
| AU709003B2 (en) | 1999-08-19 |
| SK155797A3 (en) | 1998-07-08 |
| PL323475A1 (en) | 1998-03-30 |
| YU29796A (en) | 1999-06-15 |
| NO975280L (en) | 1998-01-16 |
| NO975280D0 (en) | 1997-11-18 |
| EP0827536A1 (en) | 1998-03-11 |
| HUP9802557A2 (en) | 1999-02-01 |
| AU5921996A (en) | 1996-11-29 |
| EA000785B1 (en) | 2000-04-24 |
| CN1190435A (en) | 1998-08-12 |
| HRP960222A2 (en) | 1997-12-31 |
| JPH11505707A (en) | 1999-05-25 |
| EA199700399A1 (en) | 1998-06-25 |
| WO1996036706A1 (en) | 1996-11-21 |
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