EA199700399A1 - METHOD FOR LARGE-SCALE PLASMID CLEANING - Google Patents

METHOD FOR LARGE-SCALE PLASMID CLEANING

Info

Publication number
EA199700399A1
EA199700399A1 EA199700399A EA199700399A EA199700399A1 EA 199700399 A1 EA199700399 A1 EA 199700399A1 EA 199700399 A EA199700399 A EA 199700399A EA 199700399 A EA199700399 A EA 199700399A EA 199700399 A1 EA199700399 A1 EA 199700399A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cleaning
plasmid dna
scale plasmid
disclosed
scale
Prior art date
Application number
EA199700399A
Other languages
Russian (ru)
Other versions
EA000785B1 (en
Inventor
Энн Л. Ли
Сэнгита Сагар
Original Assignee
Мерк Энд Ко., Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Мерк Энд Ко., Инк. filed Critical Мерк Энд Ко., Инк.
Publication of EA199700399A1 publication Critical patent/EA199700399A1/en
Publication of EA000785B1 publication Critical patent/EA000785B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Раскрывается способ крупномасштабного выделения и очистки плазмидной ДНК посредством крупномасштабной ферментации микробных клеток. С использованием этого способа все три формы плазмидной ДНК, а именно сверхспиральная (форма I), незамкнутая или релаксированная кольцевая (форма II) и линеаризованная (форма III), могут быть выделены отдельно. С помощью раскрытого способа получают высокоочищенную ДНК, пригодную для включения в фармацевтическую композицию.Международная заявка была опубликована вместе с отчетом о международном поиске.A method for large-scale isolation and purification of plasmid DNA by means of large-scale fermentation of microbial cells is disclosed. Using this method, all three forms of plasmid DNA, namely, superhelix (Form I), open or relaxed circular (Form II) and linearized (Form III), can be separated. Using the disclosed method, highly purified DNA suitable for inclusion in a pharmaceutical composition is prepared. The international application was published along with an international search report.

EA199700399A 1995-05-19 1996-05-15 A method for large scale isolation and purification of plasmid dna EA000785B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44611895A 1995-05-19 1995-05-19
PCT/US1996/007083 WO1996036706A1 (en) 1995-05-19 1996-05-15 A method for large scale plasmid purification

Publications (2)

Publication Number Publication Date
EA199700399A1 true EA199700399A1 (en) 1998-06-25
EA000785B1 EA000785B1 (en) 2000-04-24

Family

ID=23771380

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199700399A EA000785B1 (en) 1995-05-19 1996-05-15 A method for large scale isolation and purification of plasmid dna

Country Status (18)

Country Link
EP (1) EP0827536A1 (en)
JP (1) JPH11505707A (en)
KR (1) KR19990014924A (en)
CN (1) CN1190435A (en)
AR (1) AR003122A1 (en)
AU (1) AU709003B2 (en)
CA (1) CA2220867A1 (en)
CZ (1) CZ366197A3 (en)
EA (1) EA000785B1 (en)
HR (1) HRP960222A2 (en)
HU (1) HUP9802557A3 (en)
MX (1) MX9708967A (en)
NO (1) NO975280L (en)
NZ (1) NZ309231A (en)
PL (1) PL323475A1 (en)
SK (1) SK155797A3 (en)
WO (1) WO1996036706A1 (en)
YU (1) YU29796A (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
CA2192342A1 (en) * 1994-07-15 1996-02-01 Ann L. Lee A method for large scale plasmid purification
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2753204B1 (en) * 1996-09-11 1998-12-04 Transgene Sa PROCESS FOR THE PREPARATION OF PLASMID DNA
SE9703532D0 (en) * 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
AU4314099A (en) * 1998-06-01 1999-12-20 Immune Response Corporation, The Novel method of large scale plasmid purification
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
CA2446478C (en) 2001-01-19 2008-02-26 Drm, Dr. Muller Ag Method for treating biomass for producing cell lysate containing plasmid dna
GB0107634D0 (en) 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP4971997B2 (en) 2005-01-31 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション Purification method of plasmid DNA
JP2006238822A (en) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd Method for separating and purifying nucleic acid
DE102005059315A1 (en) * 2005-12-09 2007-06-14 Qiagen Gmbh Process for the enrichment of short-chain nucleic acids
CN100439497C (en) * 2006-05-26 2008-12-03 吉林大学 Purification method adapted to mass production of plasmid DNA
EP2081442B1 (en) * 2006-10-10 2016-08-10 TrovaGene, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
AU2008256681B2 (en) * 2007-05-23 2014-07-31 Vgxi, Inc Compositions comprising high concentration of biologically active molecules and processes for preparing the same
CN101070543B (en) * 2007-06-01 2010-12-29 广东药学院 Super spirial plasmid separation purifying process
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
EP2556158B1 (en) 2010-04-08 2019-12-18 Qiagen GmbH Chromatographic device and method for isolating and purifying nucleic acids
JP2013523143A (en) * 2010-04-08 2013-06-17 キアゲン ゲーエムベーハー Methods for isolating and purifying nucleic acids
JP5624487B2 (en) * 2011-01-31 2014-11-12 横河電機株式会社 Nucleic acid extraction method
US9493736B2 (en) 2011-04-27 2016-11-15 Merck Patent Gmbh Method for lysing cells
CN104099321A (en) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 Preparation method of lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD
CN106884011B (en) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 Combined liquid chromatography separation method for large-scale plasmid purification
CN111721871A (en) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 High-resolution detection method for plasmid supercoiled DNA content
CN112798697B (en) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 Detection method for supercoiled structure purity of lentivirus packaging system helper plasmid
CN114456940B (en) * 2022-01-23 2024-05-03 和元生物技术（上海）股份有限公司 Method for improving plasmid stability in escherichia coli cracking process

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0452457T3 (en) * 1989-11-03 1998-03-02 Univ Vanderbilt Method for in vivo removal of functional foreign genes
AU4528493A (en) * 1992-06-04 1994-01-04 Regents Of The University Of California, The In vivo gene therapy with intron-free sequence of interest
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
CA2192342A1 (en) * 1994-07-15 1996-02-01 Ann L. Lee A method for large scale plasmid purification

Also Published As

Publication number Publication date
CA2220867A1 (en) 1996-11-21
CZ366197A3 (en) 1998-04-15
AR003122A1 (en) 1998-07-08
NZ309231A (en) 1999-10-28
MX9708967A (en) 1998-03-31
KR19990014924A (en) 1999-02-25
AU709003B2 (en) 1999-08-19
SK155797A3 (en) 1998-07-08
PL323475A1 (en) 1998-03-30
YU29796A (en) 1999-06-15
NO975280L (en) 1998-01-16
NO975280D0 (en) 1997-11-18
EP0827536A1 (en) 1998-03-11
HUP9802557A2 (en) 1999-02-01
HUP9802557A3 (en) 2000-04-28
AU5921996A (en) 1996-11-29
EA000785B1 (en) 2000-04-24
CN1190435A (en) 1998-08-12
HRP960222A2 (en) 1997-12-31
JPH11505707A (en) 1999-05-25
WO1996036706A1 (en) 1996-11-21

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU