MX9708967A - A method for large scale plasmid purification. - Google Patents

A method for large scale plasmid purification.

Info

Publication number
MX9708967A
MX9708967A MX9708967A MX9708967A MX9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A
Authority
MX
Mexico
Prior art keywords
large scale
plasmid purification
scale plasmid
disclosed
plasmid dna
Prior art date
Application number
MX9708967A
Other languages
Spanish (es)
Inventor
Ann L Lee
Sangeetha Sagar
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of MX9708967A publication Critical patent/MX9708967A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A process is disclosed for the large scale isolation and purification of plasmid DNA from large scale microbial fermentations. All three forms of plasmid DNA; supercoil (form I), nicked or relaxed circle (form II), and linearized (form III), are individually isolatable using the disclosed process. Highly purified DNA suitable for inclusion in a pharmaceutical composition is provided by the disclosed process.
MX9708967A 1995-05-19 1996-05-15 A method for large scale plasmid purification. MX9708967A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44611895A 1995-05-19 1995-05-19
PCT/US1996/007083 WO1996036706A1 (en) 1995-05-19 1996-05-15 A method for large scale plasmid purification

Publications (1)

Publication Number Publication Date
MX9708967A true MX9708967A (en) 1998-03-31

Family

ID=23771380

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9708967A MX9708967A (en) 1995-05-19 1996-05-15 A method for large scale plasmid purification.

Country Status (18)

Country Link
EP (1) EP0827536A1 (en)
JP (1) JPH11505707A (en)
KR (1) KR19990014924A (en)
CN (1) CN1190435A (en)
AR (1) AR003122A1 (en)
AU (1) AU709003B2 (en)
CA (1) CA2220867A1 (en)
CZ (1) CZ366197A3 (en)
EA (1) EA000785B1 (en)
HR (1) HRP960222A2 (en)
HU (1) HUP9802557A3 (en)
MX (1) MX9708967A (en)
NO (1) NO975280L (en)
NZ (1) NZ309231A (en)
PL (1) PL323475A1 (en)
SK (1) SK155797A3 (en)
WO (1) WO1996036706A1 (en)
YU (1) YU29796A (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
ATE277175T1 (en) * 1994-07-15 2004-10-15 Merck & Co Inc A PROCESS FOR LARGE SCALE PLASMIDS CLEANING
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2753204B1 (en) * 1996-09-11 1998-12-04 Transgene Sa PROCESS FOR THE PREPARATION OF PLASMID DNA
SE9703532D0 (en) * 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
ATE309344T1 (en) 2001-01-19 2005-11-15 Mueller Drm Ag METHOD FOR PREPARING BIOMASS FOR PRODUCING CELL LYSATE CONTAINING PLASMID DNA
GB0107634D0 (en) 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP4971997B2 (en) 2005-01-31 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション Purification method of plasmid DNA
JP2006238822A (en) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd Method for separating and purifying nucleic acid
DE102005059315A1 (en) * 2005-12-09 2007-06-14 Qiagen Gmbh Process for the enrichment of short-chain nucleic acids
CN100439497C (en) * 2006-05-26 2008-12-03 吉林大学 Purification method adapted to mass production of plasmid DNA
WO2008045505A2 (en) 2006-10-10 2008-04-17 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
ES2622418T3 (en) * 2007-05-23 2017-07-06 Vgxi, Inc. Composition comprising high concentration of biologically active molecules and processes to prepare it
CN101070543B (en) * 2007-06-01 2010-12-29 广东药学院 Super spirial plasmid separation purifying process
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
JP2013528786A (en) 2010-04-08 2013-07-11 キアゲン ゲーエムベーハー Chromatographic device and method for isolating and purifying nucleic acids
JP2013523143A (en) * 2010-04-08 2013-06-17 キアゲン ゲーエムベーハー Methods for isolating and purifying nucleic acids
JP5624487B2 (en) 2011-01-31 2014-11-12 横河電機株式会社 Nucleic acid extraction method
CN103492550A (en) * 2011-04-27 2014-01-01 默克专利股份公司 Method for lysing cells
CN104099321A (en) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 Preparation method of lung cancer resistant plasmid T-VISA (VP16-Gal4-WPRE integrated systemic amplifier)-Bik (bcl-2interacting killer) DD
CN106884011B (en) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 Combined liquid chromatography separation method for large-scale plasmid purification
CN111721871A (en) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 High-resolution detection method for plasmid supercoiled DNA content
CN112798697B (en) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 Detection method for supercoiled structure purity of lentivirus packaging system helper plasmid
CN114456940B (en) * 2022-01-23 2024-05-03 和元生物技术（上海）股份有限公司 Method for improving plasmid stability in escherichia coli cracking process

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044593C (en) * 1989-11-03 2004-04-20 Kenneth L. Brigham Method of in vivo delivery of functioning foreign genes
IL105914A0 (en) * 1992-06-04 1993-10-20 Univ California Methods and compositions for in vivo gene therapy
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
ATE277175T1 (en) * 1994-07-15 2004-10-15 Merck & Co Inc A PROCESS FOR LARGE SCALE PLASMIDS CLEANING

Also Published As

Publication number Publication date
HRP960222A2 (en) 1997-12-31
CZ366197A3 (en) 1998-04-15
WO1996036706A1 (en) 1996-11-21
PL323475A1 (en) 1998-03-30
EA000785B1 (en) 2000-04-24
EP0827536A1 (en) 1998-03-11
AU709003B2 (en) 1999-08-19
YU29796A (en) 1999-06-15
HUP9802557A3 (en) 2000-04-28
NO975280D0 (en) 1997-11-18
KR19990014924A (en) 1999-02-25
NO975280L (en) 1998-01-16
NZ309231A (en) 1999-10-28
AU5921996A (en) 1996-11-29
SK155797A3 (en) 1998-07-08
JPH11505707A (en) 1999-05-25
CA2220867A1 (en) 1996-11-21
AR003122A1 (en) 1998-07-08
CN1190435A (en) 1998-08-12
HUP9802557A2 (en) 1999-02-01
EA199700399A1 (en) 1998-06-25

Similar Documents

Publication Publication Date Title
MX9708967A (en) A method for large scale plasmid purification.
EP0771355A4 (en) A method for large scale plasmid purification
AU5450998A (en) Methods for controlling above-ground plant diseases using antibiotic-producing strains of (bacillus sp.)
IL118169A (en) Process for bioconversion of a fermentable carbon source to 1, 3-propanediol by a single microorganism
AU7735196A (en) In vitro chondrogenic induction of human mesenchymal stem cells
EP0626998A4 (en) Production of viral resistant plants via introduction of untranslatable plus sense viral rna.
AU641460B2 (en) Process for the production and extraction of antioxidants from a micro-organism culture and photobioreactor for performing this process
AU7926098A (en) Methods and means for control of flowering in plants
IL157644A0 (en) Method for the purification of a protein preparation with erythropoietin activity
WO1998002550A3 (en) Microbial isoprene generation
AU6583696A (en) Methods for reducing inhibition of nucleic acid amplification
AU2967497A (en) Enhancement of gene expression
AU2151997A (en) Method for purification of a protein from a protein containing solution
AU1722997A (en) Natamycin recovery
HUP0003751A2 (en) Method for producing ergosterol and intermediate products thereof by means of recombinant yeasts
WO1996039501A3 (en) Oligonucleotides specific for human papillomavirus
CA2029201A1 (en) Process for purifying b pertussis antigens
AU5602286A (en) Continuous process for ethanol production by bacterial fermentation
AU3255897A (en) Inhibition of hiv-1 replication by antisense rna expression
AU5950398A (en) Process and plant for air separation by cryogenic distillation
EP0744363A3 (en) Method for extracting intermediate flat supports for stacks of products
WO1993018151A1 (en) Transposable element originating in bacterium of the genus brevibacterium
AU4342285A (en) Process for the isolation and purification of interferons
NO971326L (en) Process for preparation as well as for the use of acarviosyl transferase in the conversion of acarbose homologs to acarbose, and for the preparation of acarbose homologs
AU2020897A (en) An expression system for anaerobic gene expression in higher plants

Legal Events

Date Code Title Description
FG Grant or registration
MM Annulment or lapse due to non-payment of fees