CA2220867A1 - Procede de purification de plasmide effectuee a grande echelle - Google Patents

Procede de purification de plasmide effectuee a grande echelle Download PDF

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Publication number
CA2220867A1
CA2220867A1 CA002220867A CA2220867A CA2220867A1 CA 2220867 A1 CA2220867 A1 CA 2220867A1 CA 002220867 A CA002220867 A CA 002220867A CA 2220867 A CA2220867 A CA 2220867A CA 2220867 A1 CA2220867 A1 CA 2220867A1
Authority
CA
Canada
Prior art keywords
plasmid
plasmid dna
dna
large scale
anion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002220867A
Other languages
English (en)
Inventor
Ann L. Lee
Sangeetha Sagar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2220867A1 publication Critical patent/CA2220867A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un procédé d'isolation et de purification à grande échelle d'ADN plasmidique à partir de fermentations microbiennes importantes. Ce procédé permet d'isoler individuellement les trois formes d'ADN plasmidique: superhélice (forme I), cercle crénelé ou relâché (forme II) et ligne droite (forme III). Il permet également d'obtenir de l'ADN extrêmement purifié qu'on peut introduire dans une composition pharmaceutique.
CA002220867A 1995-05-19 1996-05-15 Procede de purification de plasmide effectuee a grande echelle Abandoned CA2220867A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44611895A 1995-05-19 1995-05-19
US08/446,118 1995-05-19

Publications (1)

Publication Number Publication Date
CA2220867A1 true CA2220867A1 (fr) 1996-11-21

Family

ID=23771380

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002220867A Abandoned CA2220867A1 (fr) 1995-05-19 1996-05-15 Procede de purification de plasmide effectuee a grande echelle

Country Status (18)

Country Link
EP (1) EP0827536A1 (fr)
JP (1) JPH11505707A (fr)
KR (1) KR19990014924A (fr)
CN (1) CN1190435A (fr)
AR (1) AR003122A1 (fr)
AU (1) AU709003B2 (fr)
CA (1) CA2220867A1 (fr)
CZ (1) CZ366197A3 (fr)
EA (1) EA000785B1 (fr)
HR (1) HRP960222A2 (fr)
HU (1) HUP9802557A3 (fr)
MX (1) MX9708967A (fr)
NO (1) NO975280L (fr)
NZ (1) NZ309231A (fr)
PL (1) PL323475A1 (fr)
SK (1) SK155797A3 (fr)
WO (1) WO1996036706A1 (fr)
YU (1) YU29796A (fr)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0771355B1 (fr) * 1994-07-15 2004-09-22 Merck & Co. Inc. Procede de purification plasmidique a grande echelle
US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2753204B1 (fr) * 1996-09-11 1998-12-04 Transgene Sa Procede de preparation d'adn plasmidique
SE9703532D0 (sv) * 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
AU4314099A (en) * 1998-06-01 1999-12-20 Immune Response Corporation, The Novel method of large scale plasmid purification
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
ES2251441T3 (es) 2001-01-19 2006-05-01 Drm, Dr. Muller Ag Preparacion integrada de biomasa a partir de un proceso de cultivo celular para la fabricacion de lisato celular claro, que contiene adn plasmido.
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP4971997B2 (ja) 2005-01-31 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション プラスミドｄｎａの精製方法
JP2006238822A (ja) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
DE102005059315A1 (de) * 2005-12-09 2007-06-14 Qiagen Gmbh Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren
CN100439497C (zh) * 2006-05-26 2008-12-03 吉林大学 一种适用于大规模生产质粒dna的纯化方法
WO2008045505A2 (fr) 2006-10-10 2008-04-17 Xenomics, Inc. Compositions, procédés et trousses pour isoler acides nucléiques à partir de fluides corporels au moyen d'un milieu d'échange anionique
CA2685069C (fr) * 2007-05-23 2019-01-15 Vgx Pharmaceuticals, Inc. Composition comprenant une haute concentration en molecules biologiquement actives et procedes de preparation de celle-ci
CN101070543B (zh) * 2007-06-01 2010-12-29 广东药学院 超螺旋质粒分离纯化工艺
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
WO2011124705A1 (fr) * 2010-04-08 2011-10-13 Qiagen Gmbh Procédé d'isolement et de purification d'acides nucléiques
JP2013528786A (ja) 2010-04-08 2013-07-11 キアゲン ゲーエムベーハー 核酸を単離および精製するためのクロマトグラフィーデバイスおよび方法
JP5624487B2 (ja) 2011-01-31 2014-11-12 横河電機株式会社 核酸抽出方法
ES2656947T3 (es) * 2011-04-27 2018-03-01 Merck Patent Gmbh Método para lisar células
CN104099321A (zh) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 抗肺癌T-VISA-BikDD质粒的制备方法
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
CN112798697B (zh) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 慢病毒包装系统辅助质粒的超螺旋结构纯度的检测方法
CN114456940B (zh) * 2022-01-23 2024-05-03 和元生物技术（上海）股份有限公司 一种提高大肠杆菌裂解过程中质粒稳定性的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0452457T3 (da) * 1989-11-03 1998-03-02 Univ Vanderbilt Fremgangsmåde til in vivo fjernelse af funktionelle fremmede gener
WO1993025673A1 (fr) * 1992-06-04 1993-12-23 The Regents Of The University Of California Therapie genique in vivo a l'aide d'une sequence significative sans intron
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
EP0771355B1 (fr) * 1994-07-15 2004-09-22 Merck & Co. Inc. Procede de purification plasmidique a grande echelle

Also Published As

Publication number Publication date
NZ309231A (en) 1999-10-28
NO975280L (no) 1998-01-16
EP0827536A1 (fr) 1998-03-11
HUP9802557A2 (hu) 1999-02-01
PL323475A1 (en) 1998-03-30
EA000785B1 (ru) 2000-04-24
MX9708967A (es) 1998-03-31
NO975280D0 (no) 1997-11-18
HRP960222A2 (en) 1997-12-31
JPH11505707A (ja) 1999-05-25
YU29796A (sh) 1999-06-15
AR003122A1 (es) 1998-07-08
WO1996036706A1 (fr) 1996-11-21
SK155797A3 (en) 1998-07-08
HUP9802557A3 (en) 2000-04-28
AU709003B2 (en) 1999-08-19
CZ366197A3 (cs) 1998-04-15
EA199700399A1 (ru) 1998-06-25
AU5921996A (en) 1996-11-29
CN1190435A (zh) 1998-08-12
KR19990014924A (ko) 1999-02-25

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued