DK3536776T5 - Hidtil ukendte vertebratceller og fremgangsmåder til rekombinant ekspression af et polypeptid af interesse - Google Patents
Hidtil ukendte vertebratceller og fremgangsmåder til rekombinant ekspression af et polypeptid af interesse Download PDFInfo
- Publication number
- DK3536776T5 DK3536776T5 DK19161388.4T DK19161388T DK3536776T5 DK 3536776 T5 DK3536776 T5 DK 3536776T5 DK 19161388 T DK19161388 T DK 19161388T DK 3536776 T5 DK3536776 T5 DK 3536776T5
- Authority
- DK
- Denmark
- Prior art keywords
- polypeptide
- interest
- methods
- recombinant expression
- previously unknown
- Prior art date
Links
- 241000251539 Vertebrata <Metazoa> Species 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 238000003259 recombinant expression Methods 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21109—Matriptase (3.4.21.109)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Enzymes And Modification Thereof (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985589P | 2014-04-29 | 2014-04-29 | |
| US201461994310P | 2014-05-16 | 2014-05-16 | |
| EP15739327.3A EP3137595B1 (en) | 2014-04-29 | 2015-04-29 | Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3536776T3 DK3536776T3 (da) | 2024-01-15 |
| DK3536776T5 true DK3536776T5 (da) | 2024-09-02 |
Family
ID=53682742
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK19161388.4T DK3536776T5 (da) | 2014-04-29 | 2015-04-29 | Hidtil ukendte vertebratceller og fremgangsmåder til rekombinant ekspression af et polypeptid af interesse |
| DK15739327.3T DK3137595T3 (da) | 2014-04-29 | 2015-04-29 | Hidtil ukendte vertebratceller og fremgangsmåder til rekombinant ekspression af et polypeptid af interesse |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15739327.3T DK3137595T3 (da) | 2014-04-29 | 2015-04-29 | Hidtil ukendte vertebratceller og fremgangsmåder til rekombinant ekspression af et polypeptid af interesse |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US11512335B2 (enExample) |
| EP (3) | EP4332231A3 (enExample) |
| JP (1) | JP6636453B2 (enExample) |
| KR (1) | KR102307278B1 (enExample) |
| CN (1) | CN106255749A (enExample) |
| AU (1) | AU2015254913B2 (enExample) |
| CA (1) | CA2947198C (enExample) |
| DK (2) | DK3536776T5 (enExample) |
| ES (2) | ES2968879T3 (enExample) |
| IL (1) | IL248184B (enExample) |
| LT (2) | LT3137595T (enExample) |
| MX (1) | MX368664B (enExample) |
| PL (2) | PL3137595T3 (enExample) |
| PT (2) | PT3137595T (enExample) |
| RU (1) | RU2710726C2 (enExample) |
| SG (1) | SG11201608358VA (enExample) |
| WO (1) | WO2015166427A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4332231A3 (en) | 2014-04-29 | 2024-05-29 | Novartis AG | Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest |
| EP3015475A1 (en) | 2014-10-31 | 2016-05-04 | Novartis AG | Mammalian cells expressing cytomegalovirus antigens |
| AU2018290281B2 (en) | 2017-06-22 | 2021-12-16 | Vertex Pharmaceuticals Incorporated | Modified membrane type serine protease 1 (MTSP-1) polypeptides and methods of use |
| WO2019180607A1 (en) * | 2018-03-19 | 2019-09-26 | Centre For Cellular And Molecular Platforms | A vector, a host cell and a method thereof |
| WO2020081328A1 (en) * | 2018-10-15 | 2020-04-23 | The Regents Of The University Of California | Complement component 1s (c1s) deficient cells for production of vaccines and biopharmaceutical proteins |
| PE20221416A1 (es) | 2019-09-18 | 2022-09-20 | Novartis Ag | Proteinas de fusion nkg2d y sus usos |
| US20210244821A1 (en) | 2020-02-05 | 2021-08-12 | Novartis Ag | Cho cell expressed het il-15 |
| EP4150085A1 (en) * | 2020-05-11 | 2023-03-22 | Genentech, Inc. | Complement component c1r inhibitors for treating a neurological disease, and related compositions, systems and methods of using same |
| CN115551519A (zh) * | 2020-05-11 | 2022-12-30 | 基因泰克公司 | 用于治疗神经系统疾病的补体组分c1s抑制剂以及相关的组合物、系统和使用它们的方法 |
| EP4526462A1 (en) * | 2022-05-19 | 2025-03-26 | Novartis AG | Improved protein production using mirna technology |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4766075A (en) | 1982-07-14 | 1988-08-23 | Genentech, Inc. | Human tissue plasminogen activator |
| AU572108B2 (en) | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
| US4775624A (en) | 1985-02-08 | 1988-10-04 | Eli Lilly And Company | Vectors and compounds for expression of human protein C |
| US5648254A (en) * | 1988-01-15 | 1997-07-15 | Zymogenetics, Inc. | Co-expression in eukaryotic cells |
| WO1992008796A1 (en) | 1990-11-13 | 1992-05-29 | Immunex Corporation | Bifunctional selectable fusion genes |
| US5371198A (en) * | 1991-12-16 | 1994-12-06 | Novo Nordisk A/S | Method for protection of proteolysis-susceptible protein during protein production in a fluid medium |
| AU6953394A (en) | 1993-05-21 | 1994-12-20 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| AU2001278488A1 (en) | 2000-07-25 | 2002-02-05 | Bayer Aktiengesellschaft | Regulation of human matriptase-like serine protease |
| CN100513414C (zh) * | 2001-08-27 | 2009-07-15 | 杰南技术公司 | 进行抗体表达和装配的系统 |
| MXPA05003493A (es) * | 2002-10-02 | 2005-09-30 | Catalyst Biosciences | Metodos para generar y seleccionar proteasas con especificidad alterada. |
| ATE399872T1 (de) | 2003-03-11 | 2008-07-15 | Serono Lab | Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten |
| CN102443627B (zh) * | 2004-08-10 | 2014-10-29 | 加的夫生物学有限公司 | 用于乳腺癌的预后的试剂盒 |
| ATE504602T1 (de) | 2004-12-20 | 2011-04-15 | Amgen Fremont Inc | Für humane matriptase spezifische bindungsproteine |
| US7632509B2 (en) * | 2005-07-19 | 2009-12-15 | Biosante Pharmaceuticals, Inc. | Methods to express recombinant proteins from lentiviral vectors |
| ES2628395T3 (es) | 2007-08-15 | 2017-08-02 | Bayer Pharma Aktiengesellschaft | Anticuerpo regulado por proteasa |
| ES2546086T3 (es) | 2007-12-21 | 2015-09-18 | Novartis Ag | Sistema de selección en un cultivo de células eucariotas basado en un gen receptor de folato unido a la membrana |
| US8399219B2 (en) | 2009-02-23 | 2013-03-19 | Cytomx Therapeutics, Inc. | Protease activatable interferon alpha proprotein |
| CA2753814C (en) | 2009-02-27 | 2018-07-31 | Novartis Ag | Novel selection vectors and methods of selecting eukaryotic host cells |
| US8722964B2 (en) * | 2009-04-23 | 2014-05-13 | Transposagen Biopharmaceuticals, Inc. | Genetically engineered or transgenic rats exhibiting a cancer phenotype due to a disruption of germline tumor suppressor genes |
| PT2563904E (pt) | 2010-04-26 | 2015-05-12 | Novartis Ag | Meio de cultura de células aperfeiçoado |
| ES2676412T3 (es) | 2011-06-02 | 2018-07-19 | Socpra Sciences Santé Et Humaines S.E.C. | Inhibidores de matriptasa y usos de los mismos contra infecciones por ortomixovirus |
| CN110078827A (zh) * | 2012-04-27 | 2019-08-02 | 西托姆克斯治疗公司 | 结合表皮生长因子受体的可活化的抗体其使用方法 |
| SG11201408554QA (en) * | 2012-06-22 | 2015-02-27 | Cytomx Therapeutics Inc | Anti-jagged 1/jagged 2 cross-reactive antibodies, activatable anti-jagged antibodies and methods of use thereof |
| EP4332231A3 (en) | 2014-04-29 | 2024-05-29 | Novartis AG | Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest |
| EP3015475A1 (en) * | 2014-10-31 | 2016-05-04 | Novartis AG | Mammalian cells expressing cytomegalovirus antigens |
-
2015
- 2015-04-29 EP EP23205296.9A patent/EP4332231A3/en active Pending
- 2015-04-29 PL PL15739327T patent/PL3137595T3/pl unknown
- 2015-04-29 SG SG11201608358VA patent/SG11201608358VA/en unknown
- 2015-04-29 CN CN201580021660.0A patent/CN106255749A/zh active Pending
- 2015-04-29 DK DK19161388.4T patent/DK3536776T5/da active
- 2015-04-29 LT LTEP15739327.3T patent/LT3137595T/lt unknown
- 2015-04-29 DK DK15739327.3T patent/DK3137595T3/da active
- 2015-04-29 CA CA2947198A patent/CA2947198C/en active Active
- 2015-04-29 WO PCT/IB2015/053110 patent/WO2015166427A2/en not_active Ceased
- 2015-04-29 US US15/307,389 patent/US11512335B2/en active Active
- 2015-04-29 ES ES19161388T patent/ES2968879T3/es active Active
- 2015-04-29 KR KR1020167032875A patent/KR102307278B1/ko active Active
- 2015-04-29 PT PT15739327T patent/PT3137595T/pt unknown
- 2015-04-29 LT LTEP19161388.4T patent/LT3536776T/lt unknown
- 2015-04-29 JP JP2016565268A patent/JP6636453B2/ja active Active
- 2015-04-29 EP EP19161388.4A patent/EP3536776B1/en active Active
- 2015-04-29 PT PT191613884T patent/PT3536776T/pt unknown
- 2015-04-29 PL PL19161388.4T patent/PL3536776T3/pl unknown
- 2015-04-29 AU AU2015254913A patent/AU2015254913B2/en active Active
- 2015-04-29 ES ES15739327T patent/ES2731246T3/es active Active
- 2015-04-29 EP EP15739327.3A patent/EP3137595B1/en active Active
- 2015-04-29 MX MX2016014222A patent/MX368664B/es active IP Right Grant
- 2015-04-29 RU RU2016145928A patent/RU2710726C2/ru active
-
2016
- 2016-10-05 IL IL248184A patent/IL248184B/en active IP Right Grant
-
2022
- 2022-10-26 US US17/973,773 patent/US20230323419A1/en active Pending
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