DK3523423T3 - Fremgangsmåder til målrettet differentiering af pluripotente stamceller til hla homozygote immunceller - Google Patents

Fremgangsmåder til målrettet differentiering af pluripotente stamceller til hla homozygote immunceller Download PDF

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DK3523423T3
DK3523423T3 DK17797460.7T DK17797460T DK3523423T3 DK 3523423 T3 DK3523423 T3 DK 3523423T3 DK 17797460 T DK17797460 T DK 17797460T DK 3523423 T3 DK3523423 T3 DK 3523423T3
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methods
pluripotent stem
stem cells
cells
immune cells
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Maksym A Vodyanyk
Xin Zhang
Andrew J Brandl
Deepika Rajesh
Bradley Swanson
Christie Munn
Sarah Burton
Wen Bo Wang
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Fujifilm Cellular Dynamics Inc
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DK17797460.7T 2016-10-05 2017-10-05 Fremgangsmåder til målrettet differentiering af pluripotente stamceller til hla homozygote immunceller DK3523423T3 (da)

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US201662404470P 2016-10-05 2016-10-05
US201762486895P 2017-04-18 2017-04-18
PCT/US2017/055369 WO2018067836A1 (en) 2016-10-05 2017-10-05 Methods for directed differentiation of pluripotent stem cells to hla homozygous immune cells

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US (1) US20200263139A1 (da)
EP (1) EP3523423B1 (da)
JP (2) JP7212615B2 (da)
KR (1) KR102598351B1 (da)
CN (1) CN110023491B (da)
AU (1) AU2017340644B2 (da)
BR (1) BR112019006910A2 (da)
CA (1) CA3038701A1 (da)
DK (1) DK3523423T3 (da)
IL (1) IL265810B2 (da)
MX (1) MX2019003689A (da)
WO (1) WO2018067836A1 (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2934555B1 (en) 2012-12-21 2021-09-22 Astellas Institute for Regenerative Medicine Methods for production of platelets from pluripotent stem cells
WO2018067836A1 (en) * 2016-10-05 2018-04-12 Cellular Dynamics International, Inc. Methods for directed differentiation of pluripotent stem cells to hla homozygous immune cells
WO2019094614A1 (en) * 2017-11-08 2019-05-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of preparing hematopoietic progenitor cells in vitro
EP3927353A4 (en) * 2019-02-24 2022-11-23 Gamida-Cell Ltd. METHODS FOR HOMING AND RETENTION OF GAMMA DELTA-T CELLS FOR THE PREPARATION OF CELL COMPOSITIONS FOR USE IN THERAPY
CN110106144B (zh) * 2019-05-17 2021-10-08 苏州大学 一种诱导造血干细胞向前t系细胞分化的方法和试剂盒
CN110564691A (zh) * 2019-07-19 2019-12-13 青岛华赛伯曼医学细胞生物有限公司 Hla纯合子来源的通用型car-t细胞的制备方法
CN111808812B (zh) * 2020-08-31 2020-12-15 首都医科大学附属北京友谊医院 一种用于多能干细胞向造血干细胞分化的材料和方法
US20220333074A1 (en) 2021-04-07 2022-10-20 Century Therapeutics, Inc. Compositions and Methods for Generating Alpha-Beta T Cells from Induced Pluripotent Stem Cells
JP2024519515A (ja) 2021-04-07 2024-05-15 センチュリー セラピューティクス，インコーポレイテッド 人工多能性幹細胞からガンマ－デルタｔ細胞を生成するための組成物および方法
EP4373920A1 (en) * 2021-07-19 2024-05-29 Repairon Immuno GmbH Method of producing a population of immune cells from pluripotent stem cells
CN114324126B (zh) * 2022-01-07 2024-03-19 南京鼓楼医院 一种改变早期b细胞分化的方法
CN115896016B (zh) * 2022-09-07 2023-09-12 普华赛尔生物医疗科技有限公司 培养组合物及其在培养免疫细胞中的应用
WO2024077159A1 (en) * 2022-10-05 2024-04-11 Garuda Therapeutics, Inc. B cell lineages derived from pluripotent cells
WO2024077157A2 (en) * 2022-10-05 2024-04-11 Garuda Therapeutics, Inc. Myeloid lineages derived from pluripotent cells
KR20240069648A (ko) * 2022-11-10 2024-05-20 의료법인 성광의료재단 전분화능 줄기세포에서 자연살해세포로의 분화 유도용 배지 조성물 및 이를 이용한 분화 방법
WO2024113354A1 (en) * 2022-12-02 2024-06-06 Nuwacell Biotechnologies Co., Ltd. Culture medium, coating matrix and method for expanding midbrain dopaminergic progenitor cells
CN117050941B (zh) * 2023-10-11 2024-01-26 苏州艾凯利元生物科技有限公司 一种制备自然杀伤细胞的方法

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB2197915B (en) 1986-11-19 1990-11-14 Rolls Royce Plc Improvements in or relating to fluid bearings
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US5610042A (en) 1991-10-07 1997-03-11 Ciba-Geigy Corporation Methods for stable transformation of wheat
US5556954A (en) 1992-02-13 1996-09-17 Beth Israel Hospital Boston Association Hematopoietic stem cell specific gene expression
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
DE4228457A1 (de) 1992-08-27 1994-04-28 Beiersdorf Ag Herstellung von heterodimerem PDGF-AB mit Hilfe eines bicistronischen Vektorsystems in Säugerzellen
GB9222888D0 (en) 1992-10-30 1992-12-16 British Tech Group Tomography
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
US5908782A (en) 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
AU5389096A (en) 1996-04-11 1997-10-29 Human Genome Sciences, Inc. Human hematopoietic-specific protein
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
DE10224242A1 (de) 2002-05-29 2003-12-11 Max Delbrueck Centrum Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
CA2504451A1 (en) * 2004-08-10 2006-02-10 Geron Corporation Dendritic cell vaccines for treating cancer made from embryonic stem cells
US7606580B2 (en) 2005-05-11 2009-10-20 Aol Llc Personalized location information for mobile devices
JPWO2007049749A1 (ja) 2005-10-28 2009-04-30 ディナベック株式会社 血液凝固異常の治療方法
CN102083963A (zh) * 2008-05-06 2011-06-01 先进细胞技术公司 血管瘤集落形成细胞和非移植血管瘤细胞
US8372642B2 (en) 2009-02-27 2013-02-12 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
EP3150701B1 (en) 2009-06-05 2018-10-03 FUJIFILM Cellular Dynamics, Inc. Reprogramming t cells and hematopoietic cells
AU2015200413B2 (en) * 2009-06-05 2017-08-24 FUJIFILM Cellular Dynamics, Inc. Reprogramming t cells and hematopoietic cells
WO2011016588A1 (en) 2009-08-07 2011-02-10 Kyoto University Method of efficiently establishing induced pluripotent stem cells
US8464599B2 (en) 2011-01-10 2013-06-18 GM Global Technology Operations LLC Eight speed dual clutch transmission
PL2970912T3 (pl) * 2013-03-13 2019-08-30 Wisconsin Alumni Research Foundation Sposoby i materiały do hematopoetyczno-śródbłonkowego różnicowania ludzkich pluripotentnych komórek macierzystych w zdefiniowanych warunkach
DK2981607T3 (da) * 2013-04-03 2020-11-16 Memorial Sloan Kettering Cancer Center Effektiv generering af tumormålrettede t-celler afledt af pluripotente stamceller
US20160272937A1 (en) * 2013-10-16 2016-09-22 The Brigham And Women's Hospital, Inc. Methods of generating intermediate mesoderm cells from human pluripotent stem cells
EP3068875B1 (en) 2013-11-13 2020-05-13 Cornell University Post-natal hematopoietic endothelial cells and their isolation and use
WO2015164740A1 (en) * 2014-04-24 2015-10-29 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
EP3152301B1 (en) * 2014-06-04 2021-05-05 Fred Hutchinson Cancer Research Center Expansion and engraftment of stem cells using notch 1 and/or notch 2 agonists
WO2018067836A1 (en) * 2016-10-05 2018-04-12 Cellular Dynamics International, Inc. Methods for directed differentiation of pluripotent stem cells to hla homozygous immune cells
DK3612557T3 (da) * 2017-04-18 2022-04-19 Fujifilm Cellular Dynamics Inc Antigenspecifikke immuneffektorceller

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