DK3511423T3 - Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve - Google Patents

Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve Download PDF

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DK3511423T3
DK3511423T3 DK18208823.7T DK18208823T DK3511423T3 DK 3511423 T3 DK3511423 T3 DK 3511423T3 DK 18208823 T DK18208823 T DK 18208823T DK 3511423 T3 DK3511423 T3 DK 3511423T3
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spacial
localized
optimization
detection
methods
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DK18208823.7T
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Fredrik Salmén
Jonas Frisén
Joakim Lundeberg
Patrik Ståhl
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Spatial Transcriptomics Ab
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Priority claimed from GBGB1218654.0A external-priority patent/GB201218654D0/en
Priority claimed from GB201304585A external-priority patent/GB201304585D0/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK18208823.7T 2012-10-17 2013-10-16 Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve DK3511423T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1218654.0A GB201218654D0 (en) 2012-10-17 2012-10-17 Methods and product
GB201304585A GB201304585D0 (en) 2013-03-14 2013-03-14 Methods and product
EP13780111.4A EP2909337B1 (en) 2012-10-17 2013-10-16 Methods and product for optimising localised or spatial detection of gene expression in a tissue sample

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DK3511423T3 true DK3511423T3 (da) 2021-06-07

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US (1) US9593365B2 (da)
EP (3) EP3511423B2 (da)
CA (1) CA2886974C (da)
DK (1) DK3511423T3 (da)
WO (1) WO2014060483A1 (da)

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Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2556171B1 (en) 2010-04-05 2015-09-02 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
JP6375230B2 (ja) 2012-02-27 2018-08-15 セルラー リサーチ， インコーポレイテッド 分子計数のための組成物およびキット
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
GB2525104B (en) 2013-08-28 2016-09-28 Cellular Res Inc Massively Parallel Single Cell Nucleic Acid Analysis
US9834814B2 (en) * 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CA2948951C (en) * 2014-05-14 2023-04-04 Ruprecht-Karls-Universitat Heidelberg Synthesis of double-stranded nucleic acids
KR20230070325A (ko) 2014-06-26 2023-05-22 10엑스 제노믹스, 인크. 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
GB201411624D0 (en) * 2014-06-30 2014-08-13 Ge Healthcare Uk Ltd Spatial molecular profiling of solid biological masses and profile storage
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US11535882B2 (en) 2015-03-30 2022-12-27 Becton, Dickinson And Company Methods and compositions for combinatorial barcoding
DK3901281T3 (da) 2015-04-10 2023-01-23 Spatial Transcriptomics Ab Rumligt adskilt, multipleks nukleinsyreanalyse af biologiske prøver
EP3283656A4 (en) * 2015-04-17 2018-12-05 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological molecules
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
CN108350486B (zh) 2015-07-17 2021-09-03 纳米线科技公司 在横切面组织的用户定义区域中的基因表达的同时定量
CA3176469A1 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
EP3882357B1 (en) 2015-12-04 2022-08-10 10X Genomics, Inc. Methods and compositions for nucleic acid analysis
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
EP3635135A1 (en) 2017-06-05 2020-04-15 Becton, Dickinson and Company Sample indexing for single cells
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
CN114525273A (zh) 2017-10-27 2022-05-24 10X基因组学有限公司 用于样品制备和分析的方法和系统
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US20190249248A1 (en) 2018-02-12 2019-08-15 Nanostring Technologies, Inc. Biomolecular probes and methods of detecting gene and protein expression
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019213254A1 (en) * 2018-05-02 2019-11-07 The General Hospital Corporation High-resolution spatial macromolecule abundance assessment
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
CA3105949A1 (en) 2018-07-18 2020-01-23 Max-Delbruck-Centrum Fur Molekulare Medizin In Der Helmholtz-Gemeinschaft Method for analyzing cell sample heterogeneity
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3844308A1 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3844306A2 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Increasing spatial array resolution
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
US11208648B2 (en) 2018-11-16 2021-12-28 International Business Machines Corporation Determining position and transcriptomes of biological cells
WO2020123319A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
US20220056434A1 (en) * 2018-12-31 2022-02-24 Auransa Inc. Genomics-Based Identification and Characterazition of Rare Cell Types
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020160044A1 (en) * 2019-01-28 2020-08-06 The Broad Institute, Inc. In-situ spatial transcriptomics
CN110033474B (zh) * 2019-01-30 2021-09-03 西安天伟电子系统工程有限公司 目标检测方法、装置、计算机设备和存储介质
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
EP3931354A1 (en) 2019-02-28 2022-01-05 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
EP3938537A1 (en) 2019-03-11 2022-01-19 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3938538A1 (en) * 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
EP3887542A1 (en) 2019-03-22 2021-10-06 10X Genomics, Inc. Three-dimensional spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
JP2022537069A (ja) 2019-06-21 2022-08-23 サーモ フィッシャー サイエンティフィック バルティックス ユーエービー 次世代配列決定ライブラリを調製するための核酸の標識化に有用なオリゴヌクレオチドが連結された三リン酸ヌクレオチド
US20210024919A1 (en) * 2019-07-22 2021-01-28 Massachusetts Institute Of Technology Functionalized solid support
CN114051534A (zh) 2019-07-22 2022-02-15 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
US11514575B2 (en) 2019-10-01 2022-11-29 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
US20210130881A1 (en) 2019-11-06 2021-05-06 10X Genomics, Inc. Imaging system hardware
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021097255A1 (en) 2019-11-13 2021-05-20 10X Genomics, Inc. Generating capture probes for spatial analysis
EP4062316A1 (en) 2019-11-18 2022-09-28 10X Genomics, Inc. Systems and methods for tissue classification
CA3158888A1 (en) 2019-11-21 2021-05-27 Yifeng YIN Spatial analysis of analytes
AU2020386521A1 (en) 2019-11-22 2022-06-23 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
EP4219754B1 (en) 2019-12-23 2024-05-15 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115715329A (zh) 2020-01-10 2023-02-24 10X基因组学有限公司 确定生物样品中靶核酸位置的方法
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) * 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US20230047782A1 (en) 2020-02-07 2023-02-16 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US20230081381A1 (en) 2020-02-20 2023-03-16 10X Genomics, Inc. METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
CA3168202A1 (en) * 2020-02-21 2021-08-26 Felice Alessio BAVA Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4146819A1 (en) 2020-05-04 2023-03-15 10X Genomics, Inc. Spatial transcriptomic transfer modes
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
US20230194469A1 (en) 2020-05-19 2023-06-22 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021237056A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Rna integrity analysis in a biological sample
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
EP4158054A1 (en) 2020-06-02 2023-04-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US20230279474A1 (en) 2020-06-10 2023-09-07 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4182472A1 (en) 2020-07-15 2023-05-24 DNA Script Massively parallel enzymatic synthesis of polynucleotides
WO2022025965A1 (en) 2020-07-31 2022-02-03 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
WO2022032194A1 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
WO2022032195A2 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Spatial sequencing
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022060798A1 (en) 2020-09-15 2022-03-24 10X Genomics, Inc. Methods of releasing an extended capture probe from a substrate and uses of the same
WO2022060953A1 (en) 2020-09-16 2022-03-24 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
AU2021343507A1 (en) 2020-09-18 2023-04-06 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
EP4214678A2 (en) 2020-09-18 2023-07-26 10X Genomics, Inc. Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
WO2022087273A1 (en) 2020-10-22 2022-04-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification
CN116829733A (zh) 2020-11-06 2023-09-29 10X基因组学有限公司 用于将分析物结合至捕获探针的组合物和方法
WO2022109181A1 (en) 2020-11-18 2022-05-27 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
CN112522371A (zh) * 2020-12-21 2021-03-19 广州基迪奥生物科技有限公司 一种空间转录组测序数据的分析方法
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022147005A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Methods for analyte capture determination
US20240076723A1 (en) 2020-12-30 2024-03-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
WO2022147140A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Molecular array generation using photoresist
US20220220544A1 (en) * 2021-01-08 2022-07-14 Agilent Technologies, Inc. Spatial nucleic acid detection using oligonucleotide microarrays
WO2022164615A1 (en) 2021-01-29 2022-08-04 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
AU2022256031A1 (en) 2021-04-05 2023-10-12 10X Genomics, Inc. Recombinant ligase composition and uses thereof
EP4305196A1 (en) 2021-04-14 2024-01-17 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022226057A1 (en) 2021-04-20 2022-10-27 10X Genomics, Inc. Methods for assessing sample quality prior to spatial analysis using templated ligation
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022243303A1 (en) 2021-05-19 2022-11-24 Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft Method and system for 3d reconstruction of tissue gene expression data
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US20220403462A1 (en) 2021-06-18 2022-12-22 Miltenyi Biotec B.V. & Co. KG Method of spatial sequencing of genes from tissue using padlocks with gaps on substrate
WO2022271820A1 (en) 2021-06-22 2022-12-29 10X Genomics, Inc. Spatial detection of sars-cov-2 using templated ligation
EP4352252A1 (en) 2021-07-13 2024-04-17 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
EP4370675A1 (en) 2021-08-12 2024-05-22 10X Genomics, Inc. Methods, compositions and systems for identifying antigen-binding molecules
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023044071A1 (en) 2021-09-17 2023-03-23 10X Genomics, Inc. Systems and methods for image registration or alignment
EP4155416B1 (en) 2021-09-23 2024-01-10 Miltenyi Biotec B.V. & Co. KG Method for obtaining spatial and sequencing information of m-rna from tissue
WO2023064822A1 (en) * 2021-10-14 2023-04-20 Indevr, Inc. Nucleic acid arrays for mrna characterization
WO2023076345A1 (en) 2021-10-26 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna capture
AU2022387613A1 (en) 2021-11-10 2024-05-30 10X Genomics, Inc. Methods for identification of antigen-binding molecules
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102313A1 (en) 2021-11-30 2023-06-08 10X Genomics, Inc. Systems and methods for identifying regions of aneuploidy in a tissue
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023116938A1 (zh) * 2021-12-24 2023-06-29 映泰科技有限公司 空间转录组学分析的生物芯片和其制备方法及应用
WO2023150098A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, kits, compositions, and systems for spatial analysis
WO2023150163A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from lymphatic tissue
WO2023150171A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from glioblastoma samples
US20230306593A1 (en) 2022-02-15 2023-09-28 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
WO2023172670A2 (en) 2022-03-11 2023-09-14 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
WO2023201235A2 (en) 2022-04-12 2023-10-19 10X Genomics, Inc. Compositions and methods for generating and characterizing recombinant antigen binding molecules
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
WO2023215552A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Molecular barcode readers for analyte detection
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
WO2023229982A2 (en) 2022-05-24 2023-11-30 10X Genomics, Inc. Porous structure confinement for convection suppression
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240167077A1 (en) 2022-06-29 2024-05-23 10X Genomics, Inc. Covalent attachment of splint oligonucleotides for molecular array generation using ligation
US20240076721A1 (en) 2022-06-29 2024-03-07 10X Genomics, Inc. Method of generating arrays using microfluidics and photolithography
US20240060127A1 (en) 2022-06-29 2024-02-22 10X Genomics, Inc. Methods and systems for light-controlled surface patterning using photomasks
WO2024006798A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. High definition molecular array feature generation using photoresist
WO2024006830A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Methods and compositions for patterned molecular array generation by directed bead delivery
WO2024006816A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Compositions and methods for oligonucleotide inversion on arrays
EP4376998A1 (en) 2022-06-29 2024-06-05 10X Genomics, Inc. Methods and compositions for refining feature boundaries in molecular arrays
US20240026444A1 (en) 2022-06-29 2024-01-25 10X Genomics, Inc. Compositions and methods for generating molecular arrays using oligonucleotide printing and photolithography
WO2024015862A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024015578A1 (en) 2022-07-15 2024-01-18 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
US20240052404A1 (en) 2022-08-05 2024-02-15 10X Genomics, Inc. Systems and methods for immunofluorescence quantification
WO2024036191A1 (en) 2022-08-10 2024-02-15 10X Genomics, Inc. Systems and methods for colocalization
WO2024035844A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Methods for reducing capture of analytes
WO2024044703A1 (en) 2022-08-24 2024-02-29 10X Genomics, Inc. Compositions and methods for antigenic epitope mapping in biological samples
WO2024081212A1 (en) 2022-10-10 2024-04-18 10X Genomics, Inc. In vitro transcription of spatially captured nucleic acids
US20240191297A1 (en) 2022-10-14 2024-06-13 10X Genomics, Inc. Methods, compositions, and systems for assessing biological sample quality
WO2024086167A2 (en) 2022-10-17 2024-04-25 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
ATE152831T1 (de) 1991-09-16 1997-05-15 Molecular Probes Inc Dimere unsymmetrische cyaninfarbstoffe
US5321130A (en) 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5410030A (en) 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
JP4325114B2 (ja) 1997-12-22 2009-09-02 日立化成工業株式会社 オリゴヌクレオチド固定化ｐｃｒマイクロプレート上での直接ｒｔ−ｐｃｒ
JP5213202B2 (ja) * 1998-10-28 2013-06-19 バイシス・インコーポレーテツド 細胞アレイならびに遺伝障害マーカーの検出および使用方法
DE69913092T2 (de) 1999-01-27 2004-09-09 Commissariat à l'Energie Atomique Microassay zur Serienanalyse der Genexpression und Anwendungen davon
ES2269171T3 (es) * 1999-07-26 2007-04-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Heal Dispositivo en capas con regiones de captura para analisis celular.
EP1238286A1 (en) 1999-12-13 2002-09-11 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES High-throughput tissue microarray technology and applications
US20020168639A1 (en) * 2000-09-22 2002-11-14 Muraca Patrick J. Profile array substrates
US20030017451A1 (en) 2000-12-21 2003-01-23 Hui Wang Methods for detecting transcripts
EP1385999B1 (en) 2001-04-30 2012-12-12 Ventana Medical Systems, Inc. Reagents and methods for automated in situ or microarray hybridization
AU2002322457A1 (en) 2001-06-28 2003-03-03 Illumina, Inc. Multiplex decoding of array sensors with microspheres
US6696271B2 (en) * 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins
US20030170637A1 (en) 2002-03-06 2003-09-11 Pirrung Michael C. Method of analyzing mRNA splice variants
US20040067492A1 (en) 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US20050037362A1 (en) 2003-08-11 2005-02-17 Eppendorf Array Technologies, S.A. Detection and quantification of siRNA on microarrays
US20070178503A1 (en) 2005-12-19 2007-08-02 Feng Jiang In-situ genomic DNA chip for detection of cancer
US20070254305A1 (en) 2006-04-28 2007-11-01 Nsabp Foundation, Inc. Methods of whole genome or microarray expression profiling using nucleic acids prepared from formalin fixed paraffin embedded tissue
US20100035249A1 (en) 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
WO2010026950A1 (ja) 2008-09-08 2010-03-11 株式会社日立製作所 Ｄｎａ検出装置、及びｄｎａ検出デバイス、並びにｄｎａ検出方法
CN102639716A (zh) † 2009-12-04 2012-08-15 株式会社日立制作所 使用二维cDNA文库的基因表达解析方法
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
JP5997278B2 (ja) † 2012-07-30 2016-09-28 株式会社日立製作所 タグ配列付き２次元ｃＤＮＡライブラリーデバイス、並びにこれを用いた遺伝子発現解析方法及び遺伝子発現解析装置

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