DK3386550T3 - Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer - Google Patents

Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer Download PDF

Info

Publication number
DK3386550T3
DK3386550T3 DK16873782.3T DK16873782T DK3386550T3 DK 3386550 T3 DK3386550 T3 DK 3386550T3 DK 16873782 T DK16873782 T DK 16873782T DK 3386550 T3 DK3386550 T3 DK 3386550T3
Authority
DK
Denmark
Prior art keywords
preparing
methods
nucleic acids
guide nucleic
guide
Prior art date
Application number
DK16873782.3T
Other languages
Danish (da)
English (en)
Inventor
Stephane B Gourguechon
Original Assignee
Arc Bio Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arc Bio Llc filed Critical Arc Bio Llc
Application granted granted Critical
Publication of DK3386550T3 publication Critical patent/DK3386550T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK16873782.3T 2015-12-07 2016-12-07 Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer DK3386550T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562264262P 2015-12-07 2015-12-07
US201662298963P 2016-02-23 2016-02-23
PCT/US2016/065420 WO2017100343A1 (en) 2015-12-07 2016-12-07 Methods and compositions for the making and using of guide nucleic acids

Publications (1)

Publication Number Publication Date
DK3386550T3 true DK3386550T3 (da) 2021-04-26

Family

ID=59013518

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16873782.3T DK3386550T3 (da) 2015-12-07 2016-12-07 Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer

Country Status (8)

Country Link
US (3) US10787662B2 (enExample)
EP (2) EP3871695A1 (enExample)
JP (3) JP6995751B2 (enExample)
CN (1) CN109310784B (enExample)
AU (1) AU2016365720B2 (enExample)
CA (1) CA3006781A1 (enExample)
DK (1) DK3386550T3 (enExample)
WO (1) WO2017100343A1 (enExample)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LT3105328T (lt) 2014-02-11 2020-07-10 The Regents Of The University Of Colorado, A Body Corporate Daugiakomponentis genomo konstravimas, sudarantis sąlygas crispr
AU2016365720B2 (en) 2015-12-07 2020-11-26 Arc Bio, Llc Methods and compositions for the making and using of guide nucleic acids
CN109688820B (zh) 2016-06-24 2023-01-10 科罗拉多州立大学董事会（法人团体） 用于生成条形码化组合文库的方法
JP7383478B2 (ja) * 2016-12-22 2023-11-20 インテリア セラピューティクス，インコーポレイテッド アルファ１アンチトリプシン欠乏症を治療するための組成物および方法
EP3635114B1 (en) * 2017-06-07 2024-11-06 Arc Bio, LLC Creation and use of guide nucleic acids
IT201700067084A1 (it) * 2017-06-16 2018-12-16 Univ Degli Studi Di Palermo Metodo in vitro per l’arricchimento in sequenze genomiche bersaglio mediante sistema CRISPR-Cas
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
EP3640333A4 (en) * 2017-07-14 2020-12-30 Cure Genetics Co., Ltd GENE EDITING SYSTEM AND GENE EDITING METHOD
AU2018320864B2 (en) 2017-08-22 2024-02-22 Napigen, Inc. Organelle genome modification using polynucleotide guided endonuclease
CN112654710A (zh) 2018-05-16 2021-04-13 辛瑟高公司 用于指导rna设计和使用的方法和系统
AU2019282812B2 (en) 2018-06-07 2025-08-14 Arc Bio, Llc Compositions and methods for making guide nucleic acids
MX2021001070A (es) * 2018-07-31 2021-05-27 Intellia Therapeutics Inc Composiciones y métodos para editar el gen hidroxiácido oxidasa 1 (hao1) para tratar la hiperoxaluria primaria tipo 1 (ph1).
KR102200109B1 (ko) * 2018-09-12 2021-01-08 한국과학기술정보연구원 표적 서열 증폭용 올리고 뉴클레오타이드 및 이를 이용한 표적 서열의 증폭 방법
EP3861135B1 (en) 2018-10-04 2023-08-02 Arc Bio, LLC Normalization controls for managing low sample inputs in next generation sequencing
ES2927412T3 (es) 2018-11-08 2022-11-04 Siemens Healthcare Gmbh Secuenciación directa de nanoporos de ARN con la ayuda de un polinucleótido de horquilla
EP4317950B1 (en) 2019-03-18 2026-01-28 Regeneron Pharmaceuticals, Inc. Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation
JP7389135B2 (ja) 2019-03-18 2023-11-29 リジェネロン・ファーマシューティカルズ・インコーポレイテッド タウ凝集に関連する遺伝的脆弱性を明らかにするためのｃｒｉｓｐｒ／ｃａｓドロップアウトスクリーニングプラットフォーム
AU2020272770B2 (en) 2019-04-09 2026-01-22 Arc Bio, Llc Compositions and methods for nucleotide modification-based depletion
KR20220024053A (ko) 2019-06-14 2022-03-03 리제너론 파마슈티칼스 인코포레이티드 타우병증의 모델
AU2022272723A1 (en) * 2021-05-14 2023-11-30 Becton, Dickinson And Company Methods for making libraries for nucleic acid sequencing
CN114293264B (zh) * 2021-12-21 2024-12-27 翌圣生物科技(上海)股份有限公司 酶法靶序列随机sgRNA文库的制备方法
CN114277447A (zh) * 2021-12-21 2022-04-05 翌圣生物科技(上海)股份有限公司 靶序列随机sgRNA全覆盖组的制备方法
WO2023122762A1 (en) 2021-12-22 2023-06-29 Camp4 Therapeutics Corporation Modulation of gene transcription using antisense oligonucleotides targeting regulatory rnas
WO2024187091A1 (en) * 2023-03-08 2024-09-12 Seek Labs, Inc. Compositions and methods of isothermal nucleic acid amplification
CN116617412A (zh) * 2023-04-20 2023-08-22 浙江工业大学 一种靶向编辑mtDNA的CRISPR-Cas9递送载体的制备方法
EP4771158A1 (en) * 2023-09-01 2026-07-08 Script Biosciences, Inc. Compositions and methods for targeted modification of msh3

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8476011B1 (en) * 1996-06-18 2013-07-02 Bp Corporation North America Inc. Construction and use of catalogued nucleic acid libraries that contain advantageously adjusted representations of defined components
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US8323930B2 (en) 2007-07-28 2012-12-04 Dna Twopointo, Inc. Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
EP2393940B1 (en) 2009-02-03 2014-12-17 New England Biolabs, Inc. Generation of random double-strand breaks in dna using enzymes
JP5780971B2 (ja) 2009-03-09 2015-09-16 ニユー・イングランド・バイオレイブス・インコーポレイテツド 酵素を使用するｄｎａにおけるランダム二本鎖切断
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
DK2500436T3 (en) * 2011-03-17 2016-08-29 Pasteur Institut A method, probe and kit for in situ hybridization of DNA and use thereof
ES2728782T3 (es) 2012-05-25 2019-10-28 Univ California Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN
KR20150023670A (ko) * 2012-06-12 2015-03-05 제넨테크, 인크. 조건적 녹아웃 대립유전자의 생성 방법 및 이를 위한 조성물
KR102146721B1 (ko) 2012-07-13 2020-08-21 엑스-켐, 인크. 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리
CA2897390A1 (en) * 2013-01-10 2014-07-17 Ge Healthcare Dharmacon, Inc. Templates, libraries, kits and methods for generating molecules
CN103233028B (zh) * 2013-01-25 2015-05-13 南京徇齐生物技术有限公司 一种无物种限制无生物安全性问题的真核生物基因打靶方法及螺旋结构dna序列
US9234213B2 (en) * 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
WO2014165549A1 (en) 2013-04-01 2014-10-09 University Of Florida Research Foundation, Incorporated Determination of methylation state and chromatin structure of target genetic loci
US9873907B2 (en) 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
CA2913236A1 (en) 2013-06-07 2014-12-11 Keygene N.V. Method for targeted sequencing
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CN106133138A (zh) * 2013-08-22 2016-11-16 先锋国际良种公司 使用引导多核苷酸/cas内切核酸酶系统的基因组修饰及其使用方法
WO2015048690A1 (en) * 2013-09-27 2015-04-02 The Regents Of The University Of California Optimized small guide rnas and methods of use
EP3052651B1 (en) 2013-10-01 2019-11-27 Life Technologies Corporation Systems and methods for detecting structural variants
WO2015050501A1 (en) * 2013-10-03 2015-04-09 Agency For Science, Technology And Research Amplification paralleled library enrichment
WO2015089473A1 (en) * 2013-12-12 2015-06-18 The Broad Institute Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
JP6416939B2 (ja) * 2014-02-13 2018-10-31 タカラ バイオ ユーエスエー，インコーポレイティド 核酸の初期収集物から標的分子を減損させる方法、並びにそれを実施するための組成物及びキット
ES2898460T3 (es) * 2014-02-27 2022-03-07 Monsanto Technology Llc Composiciones y procedimientos para la modificación genómica dirigida al sitio
EP3142706A1 (en) * 2014-05-16 2017-03-22 Vrije Universiteit Brussel Genetic correction of myotonic dystrophy type 1
US20160053304A1 (en) 2014-07-18 2016-02-25 Whitehead Institute For Biomedical Research Methods Of Depleting Target Sequences Using CRISPR
US10435685B2 (en) * 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
AU2015364286B2 (en) 2014-12-20 2021-11-04 Arc Bio, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using CRISPR/Cas system proteins
AU2016211517B2 (en) 2015-01-28 2021-05-13 Caribou Biosciences, Inc. CRISPR hybrid DNA/RNA polynucleotides and methods of use
EP3250689B1 (en) * 2015-01-28 2020-11-04 The Regents of The University of California Methods and compositions for labeling a single-stranded target nucleic acid
WO2016133764A1 (en) 2015-02-17 2016-08-25 Complete Genomics, Inc. Dna sequencing using controlled strand displacement
US20160362680A1 (en) 2015-05-15 2016-12-15 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
CA3001683C (en) 2015-06-05 2024-06-04 The Regents Of The University Of California Methods and compositions for generating crispr/cas guide rnas
EP3985111A1 (en) 2015-08-19 2022-04-20 Arc Bio, LLC Capture of nucleic acids using a nucleic acid-guided nuclease-based system
AU2016335698B2 (en) 2015-10-08 2022-12-01 President And Fellows Of Harvard College Multiplexed genome editing
EP3374507A1 (en) 2015-11-09 2018-09-19 IFOM Fondazione Istituto Firc di Oncologia Molecolare Crispr-cas sgrna library
AU2016365720B2 (en) 2015-12-07 2020-11-26 Arc Bio, Llc Methods and compositions for the making and using of guide nucleic acids
CA3015360A1 (en) 2016-02-23 2017-08-31 Arc Bio, Llc Methods and compositions for target detection
EP3635114B1 (en) 2017-06-07 2024-11-06 Arc Bio, LLC Creation and use of guide nucleic acids

Also Published As

Publication number Publication date
EP3871695A1 (en) 2021-09-01
US10787662B2 (en) 2020-09-29
EP3386550B1 (en) 2021-01-20
AU2016365720A1 (en) 2018-06-14
CA3006781A1 (en) 2017-06-15
JP2021141904A (ja) 2021-09-24
US20190270984A1 (en) 2019-09-05
US20250230432A1 (en) 2025-07-17
CN109310784B (zh) 2022-08-19
US20210207130A1 (en) 2021-07-08
AU2016365720B2 (en) 2020-11-26
WO2017100343A1 (en) 2017-06-15
CN109310784A (zh) 2019-02-05
JP2018535689A (ja) 2018-12-06
EP3386550A4 (en) 2019-09-18
JP6995751B2 (ja) 2022-02-04
JP2024051149A (ja) 2024-04-10
EP3386550A1 (en) 2018-10-17

Similar Documents

Publication Publication Date Title
DK4008782T3 (da) Fremgangsmåder til tilvejebringelse af enkeltstrenget rna
DK3390631T3 (da) Fremgangsmåder og sammensætninger til t-rna-baseret guide-rna-ekspression
LT3334841T (lt) Nukleorūgščių tyrimo būdai
DK3325669T3 (da) Sammensætninger og fremgangsmåder til RNA-analyse
DK3390632T3 (da) Fremgangsmåder og sammensætninger til polymerase ii (pol-ii)-baseret guide-rna-ekspression
DK3359281T3 (da) Fremgangsmåde til udtrækning af CO2
DK3294885T3 (da) Fremgangsmåde til at fremstille rna
DK3456846T3 (da) Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre
DK3280441T3 (da) Anti-sortilin-antistoffer og fremgangsmåder til anvendelse deraf
DK3789042T3 (da) Fremgangsmåde til fremstilling af anti-her3-antistof-lægemiddelkonjugat
DK3227336T3 (da) Anti-CD79b-antistoffer og fremgangsmåder til anvendelse
DK3058092T3 (da) Fremgangsmåder og præparater til fremstilling af nukleinsyrebiblioteker
DK3294700T3 (da) Fremgangsmåde til fremstilling af limonen-4-ol
DK3483593T3 (da) Luminescent-oxygen-channeling-immunoassay ved anvendelse af tre antistoffer og fremgangsmåder til fremstilling og anvendelse deraf
EP3287793A4 (en) AUTO ANALYZER AND METHOD
DK3167076T3 (da) Fremgangsmåder og produkter til kvantificering af RNA-transkriptvarianter
DK3322734T3 (da) Multivalente og multispecifikke DR5-bindende fusionsproteiner
DK3397748T3 (da) Fremgangsmåde til dyrkning og konservering af Eubacterium hallii
DK3495482T3 (da) Fremgangsmåde til fremstilling af dna-enhedssammensætning og fremgangsmåde til frembringelse af concatenat-dna
DK3314010T3 (da) Fremgangsmåde til detektering af kromosomaberrationer
DK3441381T3 (da) Fremgangsmåde til fremstilling af methanol og indretning til fremstilling af methanol
DK3265443T3 (da) Pyrrolidincarboxamidderivater og fremgangsmåde til fremstilling og anvendelse af samme
DK3325889T3 (da) Opvarmningsmodul og fremgangsmåde til fremstilling heraf
DK3663407T3 (da) Fremgangsmåde til nukleinsyresekvensering
DK3733865T3 (da) Fremgangsmåde til opnåelse af encellet mrna-sekvens