DK3147292T3 - Mærkede nukleotider - Google Patents

Mærkede nukleotider Download PDF

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Publication number
DK3147292T3
DK3147292T3 DK16193088.8T DK16193088T DK3147292T3 DK 3147292 T3 DK3147292 T3 DK 3147292T3 DK 16193088 T DK16193088 T DK 16193088T DK 3147292 T3 DK3147292 T3 DK 3147292T3
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DK
Denmark
Prior art keywords
nucleotide
nucleoside
group
nucleotides
ethoxy
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DK16193088.8T
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English (en)
Inventor
John Milton
Silke Ruediger
Xiaohai Liu
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Illumina Cambridge Ltd
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=31946334&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3147292(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US10/227,131 external-priority patent/US7057026B2/en
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
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Publication of DK3147292T3 publication Critical patent/DK3147292T3/da

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electrophonic Musical Instruments (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Steroid Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (15)

1. Nukleosid eller nukleotid med en base bundet til et detekterbart mærke via en spaltelig linker, kendetegnet ved, at den spaltelige linker indeholder en del valgt fra gruppen bestående af:
hvor X er valgt fra gruppen bestående af O, S, NH og NQ, hvor Q er en substitueret eller usubstitueret Ci-io-alkylgruppe, Y er valgt fra gruppen bestående af O, S, NH og N(allyl), T er hydrogen eller en substitueret eller usubstitueret Ci-io-alkylgruppe, og * indikerer, hvor delen er forbundet med det resterende af nukleotidet eller nukleosidet, og hvor nukleosidet eller nukleotidet omfatter en ribose- eller deoxyribosedel med en allyl- eller azidomethyl-beskyttelsesgruppe bundet til 2'- eller 3'-oxygenatomet.
2. Nukleotid eller nukleosid ifølge krav 1, hvor X er O eller S.
3. Nukleotid eller nukleosid ifølge krav 1 eller krav 2, hvor Y er O eller S.
4. Nukleotid eller nukleosid ifølge et hvilket som helst af kravene 1 til 3, hvor Y er O.
5. Nukleotid eller nukleosid ifølge et hvilket som helst foregående krav, hvor delen kan være til stede i nukleotidet eller nukleosidet i én af to retninger.
6. Nukleotid eller nukleosid ifølge et hvilket som helst foregående krav, hvor basen er et purin eller pyrimidin.
7. Nukleotid eller nukleosid ifølge et hvilket som helst foregående krav, hvor linkeren er bundet til 5’-positionen af et pyrimidin eller 7’-positionen af et purin.
8. Nukleotid eller nukleosid ifølge et hvilket som helst foregående krav, hvor basen er et deazapurin.
9. Nukleotid eller nukleosid ifølge et hvilket som helst foregående krav, hvor nukleotidet er et deoxyribonukleotidtriphosphat.
10. Nukleosid eller nukleotid ifølge et hvilket som helst foregående krav, hvor det detekterbare mærke er en fluorophor.
11. Oligonukleotid, der omfatter et nukleosid eller nukleotid ifølge et hvilket som helst af kravene 1 til 9.
12. Oligonukleotid ifølge krav 11, hvor oligonukleotidet er bundet til en fast bærer.
13. Mikroarray, der omfatter et oligonukleotid ifølge krav 12.
14. Sammensætning, der omfatter et phosphinspaltningsreagens og et oligonukleotid ifølge krav 11.
15. Anvendelse af et nukleosid eller nukleotid ifølge et hvilket som helst af kravene 1 til 9 i sekvensering.
DK16193088.8T 2002-08-23 2003-08-22 Mærkede nukleotider DK3147292T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/227,131 US7057026B2 (en) 2001-12-04 2002-08-23 Labelled nucleotides
EP09005942.9A EP2119722B1 (en) 2002-08-23 2003-08-22 Labelled nucleotides

Publications (1)

Publication Number Publication Date
DK3147292T3 true DK3147292T3 (da) 2018-12-03

Family

ID=31946334

Family Applications (4)

Application Number Title Priority Date Filing Date
DK16193088.8T DK3147292T3 (da) 2002-08-23 2003-08-22 Mærkede nukleotider
DK09005942.9T DK2119722T3 (da) 2002-08-23 2003-08-22 Mærkede nukleotider
DK03792520T DK1560838T3 (da) 2002-08-23 2003-08-22 M rkede nuklotider
DK18195197.1T DK3438116T3 (da) 2002-08-23 2003-08-22 Mærkede nukleotider

Family Applications After (3)

Application Number Title Priority Date Filing Date
DK09005942.9T DK2119722T3 (da) 2002-08-23 2003-08-22 Mærkede nukleotider
DK03792520T DK1560838T3 (da) 2002-08-23 2003-08-22 M rkede nuklotider
DK18195197.1T DK3438116T3 (da) 2002-08-23 2003-08-22 Mærkede nukleotider

Country Status (12)

Country Link
EP (5) EP1560838B1 (da)
AT (1) ATE431354T1 (da)
AU (1) AU2003259352A1 (da)
CY (3) CY1118471T1 (da)
DE (1) DE60327649D1 (da)
DK (4) DK3147292T3 (da)
ES (4) ES2694651T3 (da)
GB (1) GB2395953A (da)
HU (2) HUE055068T2 (da)
PT (3) PT2119722T (da)
SI (3) SI3438116T1 (da)
WO (1) WO2004018493A1 (da)

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HUE055068T2 (hu) 2021-10-28
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PT3147292T (pt) 2018-11-22
SI2119722T1 (sl) 2017-03-31
AU2003259352A1 (en) 2004-03-11
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PT3438116T (pt) 2021-03-23
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