DK2119722T3 - Mærkede nukleotider - Google Patents
Mærkede nukleotider Download PDFInfo
- Publication number
- DK2119722T3 DK2119722T3 DK09005942.9T DK09005942T DK2119722T3 DK 2119722 T3 DK2119722 T3 DK 2119722T3 DK 09005942 T DK09005942 T DK 09005942T DK 2119722 T3 DK2119722 T3 DK 2119722T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleotide
- nucleoside
- nucleotides
- group
- linker
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Electrophonic Musical Instruments (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Steroid Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (15)
1. Nukleosid eller nukleotid, der har en base, der er forbundet med et detekterbart mærke via en spaltelig linker, kendetegnet ved, at den spaltelige linker omfatter en allyl- eller azidogruppe og linkeren kan spaltes ved kontakt med vandopløselige phosphiner eller vandopløselige phosphinbaserede overgangsmetalkatalysatorer.
2. Nukleosid eller nukleotid ifølge krav 1, hvor nukleosidet eller nukleotidet endvidere omfatter en dideoxyribosedel.
3. Nukleosid eller nukleotid ifølge krav 1, hvor nukleosidet eller nukleotidet endvidere omfatter en ribose- eller deoxyribosedel med en hydroxylbeskyttelsesgruppe forbundet med det 2'- eller 3'-oxygenatom.
4. Nukleosid eller nukleotid ifølge krav 3, hvor linkeren og beskyttelsesgruppen kan spaltes under de samme betingelser.
5. Nukleosid eller nukleotid ifølge krav 3, hvor beskyttelsesgruppen er allyl eller azidomethyl.
6. Nukleosid eller nukleotid ifølge et hvilket som helst af kravene 1 til 5, hvor basen er et purin, pyrimidin eller deazapurin.
7. Nukleosid eller nukleotid ifølge et hvilket som helst af kravene 1 til 6, hvor det detekterbare mærke er en fluorophor.
8. Sammensætning, der omfatter fire nukleosider eller nukleotider ifølge krav 1, hvor hvert nukleosid eller nukleotid omfatter en base, der er forbundet med et detekterbart mærke via en spaltelig linker, kendetegnet ved, at den spaltelige linker omfatter en allyl- eller azidogruppe og linkeren kan spaltes ved kontakt med vandopløselige phosphiner eller vandopløselige phosphinbaserede transitionsmetalkatalysatorer, og hvert nukleosid eller nukleotid bærer et forskelligt detekterbart mærke.
9. Oligonukleotid, der omfatter nukleosid eller nukleotid ifølge et hvilket som helst af kravene 1 til 7.
10. Oligonukleotid ifølge krav 9, hvor oligonukleotidet er forbundet med et fast underlag.
11. Mikroarray, der omfatter et oligonukleotid ifølge krav 9.
12. Sammensætning, der omfatter et phosphinspaltningsreagens og et oligonukleotid ifølge krav 9, der har en base, der er forbundet med et detekterbart mærke via en spaltelig linker, hvor den spaltelige linker omfatter en allyl- eller azidogruppe.
13. Sammensætning ifølge krav 12, hvor phosphinspaltningsreagensen er et derivatiseret tri-alkyl-phosphin, der omfatter én eller flere sulfonat-, amin-, hydroxyl eller carboxylatrester.
14. Sammensætning ifølge kravene 12 eller 13, hvor den spaltelige linker omfatter en allyldel, og sammensætningen endvidere omfatter et palladiumspaltningsreagens.
15. Anvendelse af et nukleosid eller nukleotid ifølge et hvilket som helst af kravene 1 til 7 til sekvensering.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/227,131 US7057026B2 (en) | 2001-12-04 | 2002-08-23 | Labelled nucleotides |
EP03792520A EP1560838B1 (en) | 2002-08-23 | 2003-08-22 | Labelled nucleotides |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2119722T3 true DK2119722T3 (da) | 2016-12-12 |
Family
ID=31946334
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK16193088.8T DK3147292T3 (da) | 2002-08-23 | 2003-08-22 | Mærkede nukleotider |
DK09005942.9T DK2119722T3 (da) | 2002-08-23 | 2003-08-22 | Mærkede nukleotider |
DK03792520T DK1560838T3 (da) | 2002-08-23 | 2003-08-22 | M rkede nuklotider |
DK18195197.1T DK3438116T3 (da) | 2002-08-23 | 2003-08-22 | Mærkede nukleotider |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK16193088.8T DK3147292T3 (da) | 2002-08-23 | 2003-08-22 | Mærkede nukleotider |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK03792520T DK1560838T3 (da) | 2002-08-23 | 2003-08-22 | M rkede nuklotider |
DK18195197.1T DK3438116T3 (da) | 2002-08-23 | 2003-08-22 | Mærkede nukleotider |
Country Status (12)
Country | Link |
---|---|
EP (5) | EP1560838B1 (da) |
AT (1) | ATE431354T1 (da) |
AU (1) | AU2003259352A1 (da) |
CY (3) | CY1118471T1 (da) |
DE (1) | DE60327649D1 (da) |
DK (4) | DK3147292T3 (da) |
ES (4) | ES2694651T3 (da) |
GB (1) | GB2395953A (da) |
HU (2) | HUE055068T2 (da) |
PT (3) | PT2119722T (da) |
SI (3) | SI3438116T1 (da) |
WO (1) | WO2004018493A1 (da) |
Families Citing this family (105)
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2003
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- 2003-08-22 EP EP16193088.8A patent/EP3147292B1/en not_active Revoked
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- 2003-08-22 AT AT03792520T patent/ATE431354T1/de not_active IP Right Cessation
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- 2003-08-22 DE DE60327649T patent/DE60327649D1/de not_active Expired - Lifetime
- 2003-08-22 WO PCT/GB2003/003690 patent/WO2004018493A1/en not_active Application Discontinuation
- 2003-08-22 EP EP21156755.7A patent/EP3848380A1/en not_active Withdrawn
- 2003-08-22 EP EP09005942.9A patent/EP2119722B1/en not_active Revoked
- 2003-08-22 AU AU2003259352A patent/AU2003259352A1/en not_active Abandoned
- 2003-08-22 DK DK09005942.9T patent/DK2119722T3/da active
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DK1560838T3 (da) | 2009-09-07 |
ATE431354T1 (de) | 2009-05-15 |
CY1118471T1 (el) | 2017-07-12 |
EP3848380A1 (en) | 2021-07-14 |
ES2326077T3 (es) | 2009-09-30 |
DK3438116T3 (da) | 2021-03-22 |
GB0405882D0 (en) | 2004-04-21 |
EP3147292B1 (en) | 2018-09-26 |
EP2119722B1 (en) | 2016-10-26 |
WO2004018493A1 (en) | 2004-03-04 |
HUE055068T2 (hu) | 2021-10-28 |
DK3147292T3 (da) | 2018-12-03 |
PT3147292T (pt) | 2018-11-22 |
SI2119722T1 (sl) | 2017-03-31 |
AU2003259352A1 (en) | 2004-03-11 |
DE60327649D1 (de) | 2009-06-25 |
EP3438116B1 (en) | 2021-02-17 |
EP1560838B1 (en) | 2009-05-13 |
ES2604951T3 (es) | 2017-03-10 |
ES2864086T3 (es) | 2021-10-13 |
PT2119722T (pt) | 2016-12-12 |
PT3438116T (pt) | 2021-03-23 |
SI3147292T1 (sl) | 2019-01-31 |
GB2395953A (en) | 2004-06-09 |
EP1560838A1 (en) | 2005-08-10 |
EP3147292A1 (en) | 2017-03-29 |
CY1123971T1 (el) | 2022-05-27 |
HUE032483T2 (en) | 2017-09-28 |
EP3438116A1 (en) | 2019-02-06 |
ES2694651T3 (es) | 2018-12-26 |
SI3438116T1 (sl) | 2021-07-30 |
EP2119722A1 (en) | 2009-11-18 |
CY1120882T1 (el) | 2019-12-11 |
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