DK3037552T5 - IMPROVED PROCEDURES FOR NUCLEIC ACID SEQUENCE - Google Patents

IMPROVED PROCEDURES FOR NUCLEIC ACID SEQUENCE Download PDF

Info

Publication number
DK3037552T5
DK3037552T5 DK15197029.0T DK15197029T DK3037552T5 DK 3037552 T5 DK3037552 T5 DK 3037552T5 DK 15197029 T DK15197029 T DK 15197029T DK 3037552 T5 DK3037552 T5 DK 3037552T5
Authority
DK
Denmark
Prior art keywords
dna
nucleic acid
rna
strand
transposon
Prior art date
Application number
DK15197029.0T
Other languages
Danish (da)
English (en)
Other versions
DK3037552T3 (en
Inventor
Niall Anthony Gormley
Louise Fraser
Paula Kokko-Gonzales
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Application granted granted Critical
Publication of DK3037552T3 publication Critical patent/DK3037552T3/en
Publication of DK3037552T5 publication Critical patent/DK3037552T5/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
DK15197029.0T 2012-03-06 2013-03-06 IMPROVED PROCEDURES FOR NUCLEIC ACID SEQUENCE DK3037552T5 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261607418P 2012-03-06 2012-03-06
EP13714865.6A EP2823058B1 (en) 2012-03-06 2013-03-06 Improved methods of nucleic acid sequencing

Publications (2)

Publication Number Publication Date
DK3037552T3 DK3037552T3 (en) 2018-05-28
DK3037552T5 true DK3037552T5 (en) 2018-06-06

Family

ID=48050665

Family Applications (2)

Application Number Title Priority Date Filing Date
DK15197029.0T DK3037552T5 (en) 2012-03-06 2013-03-06 IMPROVED PROCEDURES FOR NUCLEIC ACID SEQUENCE
DK13714865.6T DK2823058T3 (en) 2012-03-06 2013-03-06 Improved method for labeling nucleic acid duplexes

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK13714865.6T DK2823058T3 (en) 2012-03-06 2013-03-06 Improved method for labeling nucleic acid duplexes

Country Status (12)

Country Link
US (4) US9574226B2 (OSRAM)
EP (2) EP3037552B1 (OSRAM)
JP (2) JP6068517B2 (OSRAM)
CN (1) CN104160040B (OSRAM)
AU (1) AU2013229533B2 (OSRAM)
CA (1) CA2864276C (OSRAM)
DK (2) DK3037552T5 (OSRAM)
ES (1) ES2565809T3 (OSRAM)
HK (1) HK1226105B (OSRAM)
NO (1) NO2694769T3 (OSRAM)
TR (1) TR201807138T4 (OSRAM)
WO (1) WO2013131962A1 (OSRAM)

Families Citing this family (173)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2898456C (en) 2013-03-13 2020-11-10 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014189957A2 (en) 2013-05-23 2014-11-27 The Board Of Trustees Of The Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
EP3038834B1 (en) 2013-08-30 2018-12-12 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
SG11201605344YA (en) 2013-12-30 2016-07-28 Atreca Inc Analysis of nucleic acids associated with single cells using nucleic acid barcodes
EP3094741A1 (en) * 2014-01-14 2016-11-23 Qiagen GmbH Generation of tagged dna fragments
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CN114534806B (zh) 2014-04-10 2024-03-29 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
AU2015253299B2 (en) 2014-04-29 2018-06-14 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
WO2015172080A1 (en) 2014-05-08 2015-11-12 Fluidigm Corporation Integrated single cell sequencing
US20150353989A1 (en) * 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
CA3172086A1 (en) * 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2016003814A1 (en) * 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
WO2016037358A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
KR20170066540A (ko) 2014-10-09 2017-06-14 일루미나, 인코포레이티드 액체 중 적어도 하나를 효과적으로 격리시키기 위해 비혼화성 액체를 분리하기 위한 방법 및 디바이스
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
ES2821434T3 (es) * 2014-10-14 2021-04-26 Mgi Tech Co Ltd Método para romper un ácido nucleico y añadir un adaptador por medio de transposasa y reactivo
PT3207134T (pt) * 2014-10-17 2019-09-17 Illumina Cambridge Ltd Transposição que preserva a contiguidade
CA2964799A1 (en) * 2014-10-17 2016-04-21 Illumina Cambridge Limited Contiguity preserving transposition
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
EP3271073B1 (en) 2015-03-20 2019-06-12 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
WO2016189331A1 (en) * 2015-05-28 2016-12-01 Illumina Cambridge Limited Surface-based tagmentation
WO2017007757A1 (en) 2015-07-06 2017-01-12 Illumina, Inc. Balanced ac modulation for driving droplet operations electrodes
CN115261468A (zh) 2015-07-06 2022-11-01 伊卢米纳剑桥有限公司 用于核酸扩增的样品制备
WO2017030999A1 (en) 2015-08-14 2017-02-23 Illumina, Inc. Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
SG10201912283RA (en) 2015-08-28 2020-02-27 Illumina Inc Nucleic acid sequence analysis from single cells
WO2017037078A1 (en) 2015-09-02 2017-03-09 Illumina Cambridge Limited Systems and methods of improving droplet operations in fluidic systems
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
CA3002983A1 (en) 2015-10-22 2017-04-27 Illumina, Inc. Filler fluid for fluidic devices
US11203016B2 (en) 2015-12-01 2021-12-21 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
ES2911421T3 (es) * 2015-12-08 2022-05-19 Twinstrand Biosciences Inc Adaptadores, métodos y composiciones mejorados para secuenciación dúplex
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
CN109312396A (zh) 2016-04-07 2019-02-05 伊鲁米那股份有限公司 用于构建标准化核酸文库的方法和系统
EP3449017B1 (en) 2016-04-29 2021-12-22 Board of Regents, The University of Texas System Targeted measure of transcriptional activity related to hormone receptors
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
EP3459115A4 (en) 2016-05-16 2020-04-08 Agilome, Inc. GRAPHENE FETING DEVICES, SYSTEMS, AND METHODS OF USE THEREOF FOR NUCLEIC ACID SEQUENCING
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109642219B (zh) * 2016-08-05 2021-02-26 生物辐射实验室股份有限公司 第二链引导
EP3535566B1 (en) * 2016-11-03 2023-06-07 MGI Tech Co., Ltd. Biosensor
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
IL267445B2 (en) 2016-12-22 2023-10-01 Illumina Inc An array that includes a genetic tiling primer and a non tiling entity
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
KR102607830B1 (ko) 2017-02-21 2023-12-01 일루미나, 인코포레이티드 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화
WO2018175341A1 (en) 2017-03-20 2018-09-27 Complete Genomics, Inc. Biosensors for biological or chemical analysis and methods of manufacturing the same
KR20190133711A (ko) * 2017-03-24 2019-12-03 싱가포르국립대학교 분자의 다중 검출 방법
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10784103B2 (en) 2017-09-19 2020-09-22 Mgi Tech Co., Ltd. Water level sequencing flow cell fabrication
WO2019076768A1 (en) 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As METHOD AND KIT FOR PREPARING DNA BANK
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
MY203405A (en) * 2017-12-21 2024-06-26 Illumina Inc Flow cells with hydrogel coating
CN119020348A (zh) 2018-02-05 2024-11-26 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
EP3837381A1 (en) 2018-08-15 2021-06-23 Illumina, Inc. Compositions and methods for improving library enrichment
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP4603594A3 (en) * 2018-08-28 2025-11-12 10X Genomics, Inc. Increasing spatial array resolution
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
CN118979095A (zh) 2019-02-12 2024-11-19 10X基因组学有限公司 用于加工核酸分子的方法
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
CN113574181A (zh) * 2019-03-01 2021-10-29 武汉华大医学检验所有限公司 用于rna直接建库的核酸序列、基于rna样本直接构建测序文库的方法及应用
US20220042078A1 (en) 2019-04-29 2022-02-10 Illumina, Inc. Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP3997224A1 (en) 2019-07-12 2022-05-18 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
MX2023000872A (es) 2020-08-06 2023-02-22 Illumina Inc Preparacion de genotecas de secuenciacion de arn y adn usando transposomas enlazados por globulos.
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
EP4524261A3 (en) 2021-03-18 2025-05-14 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
CN117597453A (zh) 2021-06-23 2024-02-23 伊鲁米纳公司 用于测序试剂的组合物、方法、试剂盒、料筒和系统
WO2023034076A1 (en) 2021-08-31 2023-03-09 Illumina, Inc. Flow cell with enhanced well imaging resolution
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
WO2023056328A2 (en) * 2021-09-30 2023-04-06 Illumina, Inc. Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
CN117813397A (zh) 2021-12-21 2024-04-02 因美纳有限公司 蜡-微球基质组合物及其制备和使用方法
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5523221A (en) * 1993-06-16 1996-06-04 Stratagene Method for the directional cloning of DNA
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
WO1998030575A1 (en) 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
DE69826697T2 (de) 1997-07-07 2006-02-16 Medical Research Council In vitro selektionsmethode
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2003233219A1 (en) * 2002-04-30 2003-11-17 Board Of Regents, The University Of Texas System Ssh based methods for identifying and isolating unique nucleic acid sequences
ATE469135T1 (de) 2002-05-30 2010-06-15 Scripps Research Inst Kupferkatalysierte ligierung von aziden und acetylenen
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
EP2918595B1 (en) 2003-07-05 2019-12-11 The Johns-Hopkins University Method and compositions for detection and enumeration of genetic variations
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP2018622B1 (en) 2006-03-31 2018-04-25 Illumina, Inc. Systems for sequence by synthesis analysis
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20090226975A1 (en) 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CN105018548B (zh) * 2008-10-24 2019-03-12 阿霹震中科技公司 用于修饰核酸的试剂盒
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
MX2013001799A (es) 2010-08-27 2013-05-20 Genentech Inc Metodos para la captura y secuenciacion de acido nucleico
US9029103B2 (en) * 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
DK3037536T3 (da) * 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
EP2670894B1 (en) * 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
EP2691541B1 (en) * 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences

Also Published As

Publication number Publication date
AU2013229533A1 (en) 2014-09-04
US10267804B2 (en) 2019-04-23
JP2015509371A (ja) 2015-03-30
ES2565809T3 (es) 2016-04-07
US20240117409A1 (en) 2024-04-11
EP2823058A1 (en) 2015-01-14
NO2694769T3 (OSRAM) 2018-03-03
US11834699B2 (en) 2023-12-05
TR201807138T4 (tr) 2018-06-21
CA2864276C (en) 2020-05-05
CN104160040B (zh) 2017-07-07
EP3037552A1 (en) 2016-06-29
US9574226B2 (en) 2017-02-21
CA2864276A1 (en) 2013-09-12
DK2823058T3 (en) 2016-03-21
EP3037552B1 (en) 2018-04-25
AU2013229533B2 (en) 2018-06-28
CN104160040A (zh) 2014-11-19
JP6068517B2 (ja) 2017-01-25
DK3037552T3 (en) 2018-05-28
US20150087534A1 (en) 2015-03-26
US20170212123A1 (en) 2017-07-27
JP2017079773A (ja) 2017-05-18
HK1226105B (en) 2017-09-22
JP6341984B2 (ja) 2018-06-13
WO2013131962A1 (en) 2013-09-12
HK1206072A1 (zh) 2015-12-31
US20190324042A1 (en) 2019-10-24
EP2823058B1 (en) 2016-03-02

Similar Documents

Publication Publication Date Title
DK3037552T5 (en) IMPROVED PROCEDURES FOR NUCLEIC ACID SEQUENCE
HK1226105A1 (en) Improved methods of nucleic acid sequencing
CN104968805B (zh) 固体支持物上的样品制备
AU2015345125B2 (en) Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
HK1206072B (en) Improved methods of nucleic acid sequencing
HK40114869A (en) Sample preparation on a solid support
HK40007783A (en) Sample preparation on a solid support
HK1216776B (en) Methods and solid supports for sample preparation using transposomes