DK2909305T3 - CELLS WITH IMPROVED PENTOSE CONVERSION - Google Patents

CELLS WITH IMPROVED PENTOSE CONVERSION Download PDF

Info

Publication number
DK2909305T3
DK2909305T3 DK13776502.0T DK13776502T DK2909305T3 DK 2909305 T3 DK2909305 T3 DK 2909305T3 DK 13776502 T DK13776502 T DK 13776502T DK 2909305 T3 DK2909305 T3 DK 2909305T3
Authority
DK
Denmark
Prior art keywords
cell
gene
leu
xylose
lys
Prior art date
Application number
DK13776502.0T
Other languages
Danish (da)
English (en)
Inventor
Maria Bernedina Elizabeth Jonkers
Paul Klaassen
Aloysius Wilhelmus Rudolphus Hubertus Teunissen
Jong René Marcel De
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of DK2909305T3 publication Critical patent/DK2909305T3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y404/00Carbon-sulfur lyases (4.4)
    • C12Y404/01Carbon-sulfur lyases (4.4.1)
    • C12Y404/01005Lactoylglutathione lyase (4.4.1.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22Processes using, or culture media containing, cellulose or hydrolysates thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1022Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • C12N9/92Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y202/00Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01Transketolases and transaldolases (2.2.1)
    • C12Y202/01001Transketolase (2.2.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y202/00Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01Transketolases and transaldolases (2.2.1)
    • C12Y202/01002Transaldolase (2.2.1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017Xylulokinase (2.7.1.17)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y501/00Racemaces and epimerases (5.1)
    • C12Y501/03Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03001Ribulose-phosphate 3-epimerase (5.1.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y503/00Intramolecular oxidoreductases (5.3)
    • C12Y503/01Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01006Ribose-5-phosphate isomerase (5.3.1.6)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
DK13776502.0T 2012-10-16 2013-10-15 CELLS WITH IMPROVED PENTOSE CONVERSION DK2909305T3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12188715 2012-10-16
EP13166959 2013-05-08
PCT/EP2013/071462 WO2014060377A1 (en) 2012-10-16 2013-10-15 Cells with improved pentose conversion

Publications (1)

Publication Number Publication Date
DK2909305T3 true DK2909305T3 (en) 2019-04-15

Family

ID=49354680

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13776502.0T DK2909305T3 (en) 2012-10-16 2013-10-15 CELLS WITH IMPROVED PENTOSE CONVERSION

Country Status (12)

Country Link
US (1) US10273507B2 (enExample)
EP (2) EP3492579A1 (enExample)
KR (1) KR20150070151A (enExample)
CN (2) CN112195145A (enExample)
AR (1) AR093025A1 (enExample)
BR (1) BR112015008370B1 (enExample)
DK (1) DK2909305T3 (enExample)
ES (1) ES2717682T3 (enExample)
IN (1) IN2015DN02650A (enExample)
MY (1) MY171218A (enExample)
PL (1) PL2909305T3 (enExample)
WO (1) WO2014060377A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10604773B2 (en) * 2012-10-16 2020-03-31 Dsm Ip Assets B.V. Cells with improved pentose conversion
AU2015266065B2 (en) * 2014-05-26 2021-04-01 Katholieke Universiteit Leuven, K.U.Leuven R&D Causative genes conferring acetic acid tolerance in yeast
BR112018002284A2 (pt) 2015-08-05 2018-12-11 Cargill Inc método de fermentação para produzir um bioproduto, levedura crabtree negativa geneticamente engenheirada e método para reduzir a produção de glicerol por uma levedura engenheirada
JP6990385B2 (ja) * 2017-05-16 2022-02-03 国立大学法人 長崎大学 多重遺伝子導入手法
CN107937297B (zh) * 2017-11-29 2021-04-20 大连理工大学 一株多抑制物胁迫耐受性酿酒酵母及制备方法、应用
CN111304105B (zh) * 2020-02-27 2022-05-03 南京工业大学 利用甲醇和木糖共底物产脂肪酶的基因工程菌及其应用

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014423A1 (en) 1989-05-18 1990-11-29 The Infergene Company Microorganism transformation
ES2150188T3 (es) 1989-07-07 2000-11-16 Unilever Nv Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
FR2679920A1 (fr) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation.
ES2198410T3 (es) 1993-07-23 2004-02-01 Dsm Ip Assets B.V. Cepas recombinantes de hongos filamentosos libres de gen marcador de seleccion: un metodo para obtener dichas cepas y su utilizacion.
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
US6432672B1 (en) 1997-04-11 2002-08-13 Gerardus Cornelis Maria Selten Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
RU2230790C2 (ru) 1998-05-19 2004-06-20 Дсм Н.В. Способ расширения 5-членного кольца соединения бета-лактама до 6-членного цефема
WO2000037671A2 (en) 1998-12-22 2000-06-29 Dsm N.V. Improved in vivo production of cephalosporins
JP4334352B2 (ja) 2002-01-23 2009-09-30 ロイヤル ネダルコ ベスローテン フェンノートシャップ ペントース糖の発酵
WO2003062340A1 (en) 2002-01-25 2003-07-31 Akzo Nobel N.V. Method and apparatus for gluing
SE0202090D0 (sv) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modifierd yeast consuming L-arabinose
US7232664B2 (en) 2002-05-30 2007-06-19 Natureworks Llc Fermentation process using specific oxygen uptake rates as a process control
WO2004085627A1 (en) 2003-03-26 2004-10-07 Forskarpatent I Syd Ab New saccharomyces cerevisiae strains utilizing xylose
ES2607891T3 (es) 2004-07-16 2017-04-04 Dsm Ip Assets B.V. Ingeniería metabólica de células eucariotas que fermentan xilosa
ES2601200T3 (es) 2005-01-24 2017-02-14 Dsm Ip Assets B.V. Método para producir un compuesto de interés en una célula fúngica filamentosa
EP2035561A1 (en) 2006-06-29 2009-03-18 DSMIP Assets B.V. A method for achieving improved polypeptide expression
EP2069476B1 (en) 2006-10-02 2016-08-10 DSM IP Assets B.V. Metabolic engineering of arabinose- fermenting yeast cells
US8170326B2 (en) 2007-01-03 2012-05-01 Vialogy Corp. Multi parallax exploitation for omni-directional imaging electronic eye
WO2008121701A1 (en) 2007-03-30 2008-10-09 Cargill Inc. Metabolic engineering of yeasts for the production of 1-butanol
EP2171038A2 (en) 2007-07-19 2010-04-07 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
ES2407639T3 (es) 2008-02-04 2013-06-13 Toray Industries, Inc. Procedimiento de producción de ácido láctico por fermentación continua
EP2250262B1 (en) * 2008-03-07 2015-07-29 DSM IP Assets B.V. A pentose sugar fermenting cell
WO2009109634A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
DE102008029302B4 (de) 2008-06-19 2016-08-11 Insilico Biotechnology Ag Biotechnologische Herstellung von Acrylsäure
FR2934264B1 (fr) 2008-07-22 2012-07-20 Arkema France Fabrication d'esters de vinyle a partir de matieres renouvelables, esters de vinyle obtenus et utilisations
WO2012015949A2 (en) 2010-07-29 2012-02-02 Joule Unlimited Technologies, Inc. Methods and compositions for improving yields of reduced products of photosynthetic microorganisms
EP2663645B1 (en) 2010-11-18 2014-12-17 DSM IP Assets B.V. Yeast strains engineered to produce ethanol from glycerol
WO2014003555A1 (en) * 2012-06-27 2014-01-03 Rijksuniversiteit Groningen Improved penicillin production

Also Published As

Publication number Publication date
EP2909305A1 (en) 2015-08-26
BR112015008370A2 (pt) 2021-06-15
WO2014060377A1 (en) 2014-04-24
CN104718280A (zh) 2015-06-17
US10273507B2 (en) 2019-04-30
US20150275235A1 (en) 2015-10-01
PL2909305T3 (pl) 2019-06-28
IN2015DN02650A (enExample) 2015-09-18
ES2717682T3 (es) 2019-06-24
EP3492579A1 (en) 2019-06-05
BR112015008370B1 (pt) 2022-10-18
CN112195145A (zh) 2021-01-08
AR093025A1 (es) 2015-05-13
EP2909305B1 (en) 2018-12-26
MY171218A (en) 2019-10-02
KR20150070151A (ko) 2015-06-24

Similar Documents

Publication Publication Date Title
US11649471B2 (en) Fermentative production of ethanol from glucose, galactose and arabinose employing a recombinant yeast strain
US9034608B2 (en) Polypeptides with permease activity
US9499841B2 (en) Cell suitable for fermentation of a mixed sugar composition
DK2909305T3 (en) CELLS WITH IMPROVED PENTOSE CONVERSION
US10604773B2 (en) Cells with improved pentose conversion
WO2016012429A1 (en) Yeast cell with improved pentose transport
WO2015091757A1 (en) Polypeptides with permease activity