DK2909305T3 - Celler med forbedret pentoseomdannelse - Google Patents

Celler med forbedret pentoseomdannelse Download PDF

Info

Publication number
DK2909305T3
DK2909305T3 DK13776502.0T DK13776502T DK2909305T3 DK 2909305 T3 DK2909305 T3 DK 2909305T3 DK 13776502 T DK13776502 T DK 13776502T DK 2909305 T3 DK2909305 T3 DK 2909305T3
Authority
DK
Denmark
Prior art keywords
cell
gene
leu
xylose
lys
Prior art date
Application number
DK13776502.0T
Other languages
English (en)
Inventor
Maria Bernedina Elizabeth Jonkers
Paul Klaassen
Aloysius Wilhelmus Rudolphus Hubertus Teunissen
Jong René Marcel De
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of DK2909305T3 publication Critical patent/DK2909305T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y404/00—Carbon-sulfur lyases (4.4)
    • C12Y404/01—Carbon-sulfur lyases (4.4.1)
    • C12Y404/01005—Lactoylglutathione lyase (4.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01001—Transketolase (2.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01002—Transaldolase (2.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03001—Ribulose-phosphate 3-epimerase (5.1.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01006—Ribose-5-phosphate isomerase (5.3.1.6)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)

Claims (14)

1. Saccharomyces-celle, der er i stand til at omdanne en eller flere pentose-sukkerarter og en eller flere hexose-sukkerarter til et fermenteringsprodukt, som omfatter en nukleotidsekvens, der koder for en xyloseisomerase, der bibringer evnen til at omdanne xylose ved hjælp af isomerisering eller reduktion, hvilken celle konstitutivt udtrykker et eller flere heterologe eller homologe polypeptider med aminosyresekvensen ifølge SEQ ID NO: 20 eller et variant polypeptid deraf, der har mindst 45 % identitet med SEQ ID NO: 20 og har glyoxylase I-aktivitet, hvor cellen omfatter en eller flere genmodifikationer, som resulterer i et fald i aldosereductaseaktivitet i cellen.
2. Celle ifølge krav 1, hvor det heterologe polypeptid har glyoxalase I-aktivitet.
3. Celle ifølge krav 1 eller 2, hvor cellen omfatter en eller flere genmodifikationer, som resulterer i: (a) en forøgelse af transporten af xylose i cellen; (b) en forøgelse af xylulosekinaseaktivitet; (c) en forøgelse af strømmen gennem pentosephosphatsystemet; (d) et fald i følsomheden for katabolitrepression; (e) en forøgelse af toleransen for ethanol, osmolaritet eller organiske syrer; eller (f) en reduceret produktion af biprodukter.
4. Celle ifølge krav 3, hvor den ene eller de flere genmodifikationer resulterer i overekspression af mindst ét gen, der koder for et enzym i den ikke-oxidative del af pentosephosphatsystemet.
5. Celle ifølge krav 4, hvor genet er et gen, der koder for en ribulose-5-phosphatisomerase, en ribulose-5- phosphatepimerase, en transketolase eller en transaldolase.
6. Celle ifølge et hvilket som helst af kravene 3 til 5, hvor den ene eller de flere genmodifikationer resulterer i overekspression af et gen, der koder for en xylulosekinase.
7. Celle ifølge et hvilket som helst af kravene 3 til 6, der har evnen til at anvende L-arabinose, hvor generne TALI, TKL1, RPE1 og RKI1 overudtrykkes.
8. Celle ifølge et hvilket som helst af kravene 1 til 7, hvor det kodende område af GRE3-genet er inaktiveret ved substitution af det kodende område med en nukleotidsekvens, der omfatter generne TALI, TKL1, RPE1 og RKI1.
9. Celle ifølge et hvilket som helst af kravene 1 til 8, hvor generne araA, araB og araD udtrykkes.
10. Celle ifølge et hvilket som helst af kravene 1 til 9, hvor et eller flere konstitutivt udtrykte eller konstitutivt overudtrykte gener er stabilt integreret i genomet i cellen.
11. Fremgangsmåde til frembringelse af et fermenteringsprodukt, hvilken fremgangsmåde omfatter fermentering af et medium, der indeholder en kilde af xylose og/eller arabinose, med en celle ifølge et hvilket som helst af kravene 1 til 10, således at cellen fermenterer xylose til fermenteringsproduktet.
12. Fremgangsmåde ifølge krav 11, hvor fermenteringsproduktet er valgt fra listen, der indbefatter ethanol, butanol, mælkesyre, 3-hydroxypropionsyre, acrylsyre, eddikesyre, ravsyre, citronsyre, æblesyre, fumarsyre, itaconsyre, en aminosyre, 1,3-propandiol, ethylen, glycerol, butanol, et β-lactam-antibiotikum og et cephalosporin.
13. Fremgangsmåde ifølge krav 12, hvor fermenteringsproduktet er ethanol.
14. Fremgangsmåde ifølge krav 12 eller 13, hvor fremgangsmåden er anaerob.
DK13776502.0T 2012-10-16 2013-10-15 Celler med forbedret pentoseomdannelse DK2909305T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12188715 2012-10-16
EP13166959 2013-05-08
PCT/EP2013/071462 WO2014060377A1 (en) 2012-10-16 2013-10-15 Cells with improved pentose conversion

Publications (1)

Publication Number Publication Date
DK2909305T3 true DK2909305T3 (da) 2019-04-15

Family

ID=49354680

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13776502.0T DK2909305T3 (da) 2012-10-16 2013-10-15 Celler med forbedret pentoseomdannelse

Country Status (12)

Country Link
US (1) US10273507B2 (da)
EP (2) EP3492579A1 (da)
KR (1) KR20150070151A (da)
CN (2) CN112195145A (da)
AR (1) AR093025A1 (da)
BR (1) BR112015008370B1 (da)
DK (1) DK2909305T3 (da)
ES (1) ES2717682T3 (da)
IN (1) IN2015DN02650A (da)
MY (1) MY171218A (da)
PL (1) PL2909305T3 (da)
WO (1) WO2014060377A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10604773B2 (en) * 2012-10-16 2020-03-31 Dsm Ip Assets B.V. Cells with improved pentose conversion
AU2015266065B2 (en) * 2014-05-26 2021-04-01 Katholieke Universiteit Leuven, K.U.Leuven R&D Causative genes conferring acetic acid tolerance in yeast
BR112018002284A2 (pt) 2015-08-05 2018-12-11 Cargill Inc método de fermentação para produzir um bioproduto, levedura crabtree negativa geneticamente engenheirada e método para reduzir a produção de glicerol por uma levedura engenheirada
JP6990385B2 (ja) * 2017-05-16 2022-02-03 国立大学法人 長崎大学 多重遺伝子導入手法
CN107937297B (zh) * 2017-11-29 2021-04-20 大连理工大学 一株多抑制物胁迫耐受性酿酒酵母及制备方法、应用
CN111304105B (zh) * 2020-02-27 2022-05-03 南京工业大学 利用甲醇和木糖共底物产脂肪酶的基因工程菌及其应用

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014423A1 (en) 1989-05-18 1990-11-29 The Infergene Company Microorganism transformation
IE81016B1 (en) 1989-07-07 1999-09-22 Unilever Plc Process for preparing a protein by a fungus transformed by a multicopy integration of an expression vector
FR2679920A1 (fr) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation.
EP0635574B1 (en) 1993-07-23 2003-04-23 Dsm N.V. Selection marker gene free recombinant strains, a method for obtaining them and the use of these strains
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
PL336345A1 (en) 1997-04-11 2000-06-19 Dsm Nv Genic conversion as a tool for constructing recombined filiform fungi
MXPA00011223A (es) 1998-05-19 2002-04-17 Dsm Nv Produccion mejorada in vivo de cefalosporinas.
KR20010089672A (ko) 1998-12-22 2001-10-08 윌리암 로엘프 드 보에르 개선된 생체내 세팔로스포린 생산
DE60325457D1 (de) 2002-01-23 2009-02-05 Royal Nedalco B V Fermentation von pentosezuckern
WO2003062340A1 (en) 2002-01-25 2003-07-31 Akzo Nobel N.V. Method and apparatus for gluing
SE0202090D0 (sv) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modifierd yeast consuming L-arabinose
AU2003240481B8 (en) 2002-05-30 2008-03-20 Natureworks Llc Methods and materials for the production of D-lactic acid in yeast
WO2004085627A1 (en) 2003-03-26 2004-10-07 Forskarpatent I Syd Ab New saccharomyces cerevisiae strains utilizing xylose
DK1781772T3 (da) 2004-07-16 2017-01-09 Dsm Ip Assets Bv Metabolisk modificering af xylose-fermenterende eukaryote celler
EP1841861A1 (en) 2005-01-24 2007-10-10 DSMIP Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EP2035561A1 (en) 2006-06-29 2009-03-18 DSMIP Assets B.V. A method for achieving improved polypeptide expression
MX2009003501A (es) 2006-10-02 2009-04-16 Dsm Ip Assets Bv Ingenieria metabolica de celulas eucariotas que fermentan arabinosa.
US8170326B2 (en) 2007-01-03 2012-05-01 Vialogy Corp. Multi parallax exploitation for omni-directional imaging electronic eye
WO2008121701A1 (en) 2007-03-30 2008-10-09 Cargill Inc. Metabolic engineering of yeasts for the production of 1-butanol
EP2171038A2 (en) 2007-07-19 2010-04-07 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
CN101939439B (zh) 2008-02-04 2014-08-06 东丽株式会社 通过连续发酵实施的乳酸的制造方法
AU2009221104B2 (en) * 2008-03-07 2014-07-31 Dsm Ip Assets B.V. A pentose sugar fermenting cell
CN101965400A (zh) * 2008-03-07 2011-02-02 帝斯曼知识产权资产管理有限公司 发酵戊糖的细胞
DE102008029302B4 (de) 2008-06-19 2016-08-11 Insilico Biotechnology Ag Biotechnologische Herstellung von Acrylsäure
FR2934264B1 (fr) 2008-07-22 2012-07-20 Arkema France Fabrication d'esters de vinyle a partir de matieres renouvelables, esters de vinyle obtenus et utilisations
WO2012015949A2 (en) * 2010-07-29 2012-02-02 Joule Unlimited Technologies, Inc. Methods and compositions for improving yields of reduced products of photosynthetic microorganisms
CA2834053C (en) 2010-11-18 2019-03-26 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from glycerol
WO2014003555A1 (en) * 2012-06-27 2014-01-03 Rijksuniversiteit Groningen Improved penicillin production

Also Published As

Publication number Publication date
AR093025A1 (es) 2015-05-13
EP2909305A1 (en) 2015-08-26
EP3492579A1 (en) 2019-06-05
ES2717682T3 (es) 2019-06-24
US20150275235A1 (en) 2015-10-01
PL2909305T3 (pl) 2019-06-28
CN112195145A (zh) 2021-01-08
BR112015008370B1 (pt) 2022-10-18
BR112015008370A2 (pt) 2021-06-15
KR20150070151A (ko) 2015-06-24
US10273507B2 (en) 2019-04-30
MY171218A (en) 2019-10-02
IN2015DN02650A (da) 2015-09-18
WO2014060377A1 (en) 2014-04-24
EP2909305B1 (en) 2018-12-26
CN104718280A (zh) 2015-06-17

Similar Documents

Publication Publication Date Title
US11649471B2 (en) Fermentative production of ethanol from glucose, galactose and arabinose employing a recombinant yeast strain
US9034608B2 (en) Polypeptides with permease activity
US9499841B2 (en) Cell suitable for fermentation of a mixed sugar composition
DK2909305T3 (da) Celler med forbedret pentoseomdannelse
US10604773B2 (en) Cells with improved pentose conversion
WO2016012429A1 (en) Yeast cell with improved pentose transport
WO2015091757A1 (en) Polypeptides with permease activity