DK2861728T3 - Forbedret fremstilling af polyumættede fedtsyrer ved coekspression af acyl-coa:lysophosphatidylcholinacyltransferaser og phospholipid:diacylglycerolacyltransferaser - Google Patents

Forbedret fremstilling af polyumættede fedtsyrer ved coekspression af acyl-coa:lysophosphatidylcholinacyltransferaser og phospholipid:diacylglycerolacyltransferaser Download PDF

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DK2861728T3
DK2861728T3 DK13731233.6T DK13731233T DK2861728T3 DK 2861728 T3 DK2861728 T3 DK 2861728T3 DK 13731233 T DK13731233 T DK 13731233T DK 2861728 T3 DK2861728 T3 DK 2861728T3
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Michael W Bostick
Narendra S Yadav
Hongxiang Zhang
Quinn Qun Zhu
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Du Pont
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    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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Claims (8)

  1. FORBEDRET FREMSTILLING AF POLYUMÆTTEDE FEDTSYRER VED COEKSPRESSION AF ACYL-COA:LYSOPHOSPHATIDYLCHOLINACYLTRANSFERASER OG PHOSPHOLIPID:DIACYLGLYCEROLACYLTRANSFE RASER
    1. Rekombinant Yarrowia-celle til fremstilling af mindst én langkædet, polyumættet fedtsyre med en kædelængde på C20 eller større, hvor den rekombinante celle er blevet genetisk manipuleret til at omfatte en biosyntetisk polyumættet bane, der er i stand til at fremstille mindst én langkædet, polyumættet fedtsyre og er yderligere modificeret til at indføre; (a) et kimært gen, der koder for mindst ét polypeptid med acyl CoAdysophosphatidylcholinacyltransferase- (LPCAT) aktivitet, hvor polypeptidet har mindst 90 % aminosyreidentitet, baseret på Clustal W-alignmentmetoden ved sammenligning med aminosyresekvensen ifølge SEQ ID NO: 4 (YILPCAT); og (b) et kimært gen, der koder for mindst ét polypeptid med phospholipid: diacylgiycerolacyltransferase- (PDAT) aktivitet, hvor polypeptidet har mindst 90 % aminosyreidentitet, baseret på Clustal W-alignmentmetoden, ved sammenligning med aminosyresekvensen ifølge SEQ ID NO: 32 (YIPDAT); og hvor de kimære gener hvert omfatter en promoter, der er heterolog med den kodende sekvens, der koder for polypeptiderne fra (a) og (b), og hvor den rekombinante celle omfatter en øget mængde af den polyumættede fedtsyre målt som en vægtprocent af totale fedtsyrer ved sammenligning med en kontrolcelle, der svarer til den rekombinante Yarrowia celle til fremstilling, men som ikke er modificeret, til indføring af de kimære gener, der koder for LPCAT- eller PDAT-polypeptiderne fra (a) og (b).
  2. 2. Rekombinant Yarrowia-celle ifølge krav 1, hvor den rekombinant celle endvidere omfatter mindst én af følgende ved sammenligning med kontrolcellen: (i) en øget Ci8- til C20-elongeringsomdannelseseffektivitet, eller (ii) en øget mængde af totale fedtsyrer målt som en vægtprocent af tør cellevægt.
  3. 3. Rekombinant Yarrowia-celle ifølge krav 2, hvor den øgede Ci8- til C2o- elongeringsomdannelseseffektivitet er en virkning af øget delta-9-elongaseomdannelseseffektivitet eller øget delta- 6-elongaseomdannelseseffektivitet.
  4. 4. Rekombinant Yarrowia-celle ifølge krav 1, hvor polypeptidet, der har PDAT-aktivitet, har mindst 95 % aminosyreidentitet, baseret på Clustal W-alignmentmetoden ved sammenligning med en aminosyresekvens udvalgt fra gruppen bestående af SEQ ID NO: 32 (YIPDAT), og/eller hvor polypeptidet med acyl CoAdysophosphatidylcholin-acyltransferaseaktivitet har mindst 95 % aminosyreidentitet, baseret på Clustal W-alignmentmetoden ved sammenligning med aminosyresekvensen ifølge SEQ ID NO: 4 (YILPCAT).
  5. 5. Rekombinant Yarrowia-celle ifølge krav 1, hvor polypeptidet med LPCAT-aktivitet er udvalgt fra gruppen bestående af: a) et polypeptid, der omfatter mindst ét membranbundet mønster af O-acyltransferaseproteinfamilien, der er udvalgt fra gruppen bestående af: SEQ ID NO: 5 (WHG-X3-GY-X3-F), SEQ ID NO: 6 (Y-X4-F), SEQ ID NO: 7 (Y-X3-YF-X2-H), SEQ ID NO: 8 (M-[V/I]-[L/I]-X2-K-[L/V/I]- Xs-DG), SEQ ID NO: 9 (RxKYY-X2-W-X3-[E/D]-[A/G]-X5-GxG-[F/Y]-xG), SEQ ID NO: 10 (EXUWN-X2-[T/V]-X2-W), SEQ ID NO: 11 (SAxWHG-X2-PGY-X2-[T/F]-F), SEQ ID NO: 12 (M-[V/I]-[L/I/V]-[V/C/A/T]-[M/L/Q]-K-[IW/I/M]-[S/T/Y/I]-[S/T/A/M/G]-[F/L/CA^]-[C/A/G/S]-[W/Y/M/I/F/C]-[N/S/E/Q/D]-[V/Y/L/I]-[H/Y/A/N/S/T]-DG), SEQ ID NO: 13 (R-[L/M/F/W/P/Y]-KYY-[G/A/F/H/S]-[V/A/EC]-W-[Y/E/T/M/S/L]-[L/I/N]-[T/S/A]-[E/D]-[G/A]-[A/S/EV]-[C/S/I/N/H/L]-[V/I/N]-[L/I/N/A/C]-[S/CAV/A/I]-G-[M/I/L/A/F]-G-[Y/F]-[N/E/S/T/R/K]-G), SEQ ID NO: 14 (E-[T/F/L/M]-[A/S]- [Q/D/P/K/T]-[N/S]-[S/I/T/L/A/M/F]-[H/K/R/V]-[G/C/E/T/Q/D/M]-[Y/A/M/L/I/F]-[L/S/P/I]-[G/E/A/L/N/D]-[S/A/V/F/M/N]-WN-[K/M/I/C]-[N/K/Q/G]-[TA?]-[N/A/S]-[H/K/N/T/R/L]-W), SEQ ID NO: 15 (SA-[F/M/V/I]-WHG-[F/V/T/L] [Y/S/R]-PGY-[Y/M/I]-[L/M/I/F]-[T/F]-F), SEQ ID NO: 16 (M-[V/I]-L-X2-KL), SEQ ID NO: 17 (RxKYY-X2-W) og SEQ ID NO: 18 (SAxWHG); og (b) et polypeptid, der omfatter mindst ét mutant membranbundet mønster af O-acyltransferaseproteinfamilien, der er udvalgt fra gruppen bestående af: (i) et mutantmønster, der omfatter en aminosyresekvens ifølge SEQ ID NO: 38, hvor SEQ ID NO: 38 adskiller sig fra SEQ ID NO: 16 (M-[V/I]-L-X2-KL) ved mindst én aminosyremutation udvalgt fra gruppen bestående af: V2C, I2C, L3A, L3C, L3G, K6H, K6G, K6N, K6Y, L7A, L7N, L7G, L7H, L7I og L7M; (ii) et mutantmønster, der omfatter en aminosyresekvens ifølge SEQ ID NO: 39, hvor SEQ ID NO: 39 adskiller sig fra SEQ ID NO: 8 (M-[V/I]-[L/I]-X2-K-[L/V/I]-X8-DG) ved mindst én aminosyremutation udvalgt fra gruppen bestående af: V2C, I2C, L3A, L3C, L3G, I3A, I3C, I3G, K6H, K6G, K6N, K6Y, L7A, L7N, L7G, L7H, L7M, V7A, V7N, V7G, V7H, V7M, 17A, I7N, I7G, I7H, I7M, D16Q, D16N, D16H, G17A, G17V og G17N; (iii) et mutantmønster, der omfatter en aminosyresekvens ifølge SEQ ID NO: 40, hvor SEQ ID NO: 40 adskiller sig fra SEQ ID NO: 5 (WHG-X3-GY-X3-F) med mindst én aminosyremutation udvalgt fra gruppen bestående af: F12N, F12C, F12G og F12T; og (iv) et mutantmønster, der omfatter en aminosyresekvens ifølge SEQ ID NO: 41, hvor SEQ ID NO: 41 adskiller sig fra SEQ ID NO: 11 (SAxWHG-X2-PGY-X2-[T/F]-F) med mindst én aminosyremutation udvalgt fra gruppen bestående af: T14A, T14C, T14S, F14A, F14C, F14S, F15N, F15C, F15G og F15T.
  6. 6. Rekombinant Yarrowia-celle ifølge et hvilket som helst af kravene 1 til 5, hvor den langkædede, polyumættede fedtsyre er udvalgt fra gruppen bestående af: eicosadiensyre, dihomo-gamma-linolensyre, arachidonsyre, docosatetraensyre, omega-6-docosapentaensyre, eicosatriensyre, eicosatetraensyre, eicosapentaensyre, omega-3-docosapentaensyre og docosahexaensyre.
  7. 7. Fremgangsmåde til forbedring af fremstillingen af mindst én langkædet, polyumættet fedtsyre med en kædelængde på C20 eller større, hvilken fremgangsmåde omfatter: (a) dyrkning af den rekombinante mikrobielle celle ifølge et hvilket som helst af kravene 1 til 6 i nærvær af en fermenterbar carbonkilde; og (b) eventuel genvinding af den langkædede, polyumættede fedtsyre.
  8. 8. Fremgangsmåde ifølge krav 7, hvor den langkædede, polyumættede fedtsyre er udvalgt fra gruppen bestående af: eicosadiensyre, dihomo-gamma-linolensyre, arachidonsyre, docosatetraensyre, omega-6-docosapentaensyre, eicosatriensyre, eicosatetraensyre, eicosapentaensyre, omega-3-docosapentaensyre og docosahexaensyre.
DK13731233.6T 2012-06-19 2013-06-13 Forbedret fremstilling af polyumættede fedtsyrer ved coekspression af acyl-coa:lysophosphatidylcholinacyltransferaser og phospholipid:diacylglycerolacyltransferaser DK2861728T3 (da)

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PCT/US2013/045592 WO2013192002A1 (en) 2012-06-19 2013-06-13 IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES

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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6351582B2 (ja) * 2012-06-19 2018-07-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ アシルＣｏＡ：リゾホスファチジルコリンアシルトランスフェラーゼおよびリン脂質：ジアシルグリセロールアシルトランスフェラーゼの同時発現による多価不飽和脂肪酸の産生の改善
DK2935601T3 (da) * 2012-12-21 2018-06-18 Du Pont Rekombinante mikrobeceller, der fremstiller mindst 28 % eicosapentaensyre som tørvægt
JP2017195777A (ja) * 2014-08-29 2017-11-02 国立大学法人京都大学 エイコサペンタエン酸を高含有する脂質の生産方法
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
CN115820586A (zh) * 2022-09-06 2023-03-21 中国农业科学院油料作物研究所 一种溶血磷脂酰胆碱酰基转移酶、核酸分子及其应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
SK139999A3 (en) 1997-04-11 2000-05-16 Calgene Llc A nucleic acids construct, isolated nucleotide sequence, recombinant plant cell, method of biosynthesis of polyunsaturated long chain fatty acids, pharmaceutical, food and cosmetic preparation and use thereof
UA75041C2 (en) * 1999-04-01 2006-03-15 Basf Plant Science Gmbh Use of ferment of phospholipiddiacyglycerintransferase as catalyst in the process of biosynthetic producing triacylglycerin
TWI324181B (en) 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
EP1599582B1 (de) 2003-02-27 2011-07-27 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren
AU2004225838B2 (en) 2003-03-31 2009-11-05 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US8313911B2 (en) 2003-05-07 2012-11-20 E I Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
DE102004062294A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
US20090099343A1 (en) 2005-01-13 2009-04-16 David Peretz Isolation of pathogenic prions
KR101482081B1 (ko) 2005-03-18 2015-01-13 마이크로비아 인크. 유질 효모와 진균 내에서 카로티노이드의 생산
EP1906725A4 (en) 2005-05-20 2010-04-28 Univ Washington State IMPROVING ACCUMULATION OF HYDROXY FATTY ACIDS IN OILSEED PLANTS
US7732155B2 (en) 2006-12-13 2010-06-08 National Research Council Of Canada Methods for identifying lysophosphatidylcholine acyltransferases
ES2559312T3 (es) 2007-04-03 2016-02-11 E. I. Du Pont De Nemours And Company Multienzimas y su uso en la fabricación de ácidos grasos poliinsaturados
WO2009001315A2 (en) 2007-06-26 2008-12-31 Staahl Ulf Use of a class of genes encoding lysophospholipid acyl transferases for application in agriculture, biotechnology, and medicine
CA2707372C (en) 2007-07-23 2015-11-24 Suntory Holdings Limited Fatty acid compositions having novel fatty acid rate
EP2195415A1 (en) 2007-10-03 2010-06-16 E. I. du Pont de Nemours and Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
CN102170774A (zh) 2008-04-25 2011-08-31 联邦科学技术研究组织 用于产生包含修饰的脂肪酸的三酰基甘油的多肽及方法
BRPI0913714A2 (pt) * 2008-09-19 2020-10-13 E. I. Du Pont De Nemours And Company polipeptídeo mutante que possui atividade de a5 dessaturase, molécula de ácido nucleico isolada, célula hospedeira microbiana e métodos de produção de ácido araquidônico e de ácido eicosapentaenoico
US8524485B2 (en) 2009-06-16 2013-09-03 E I Du Pont De Nemours And Company Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases
CA2763424C (en) * 2009-06-16 2018-06-12 E.I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-coa lysophospholipid acyltransferases
CA2761589C (en) * 2009-06-16 2017-12-19 E. I. Du Pont De Nemours And Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
BR112013004351A2 (pt) 2010-08-26 2016-05-31 Du Pont célula hospedeira microbiana recombinante e método para a produção de um óleo microbiano compreendendo ácido eicosapentaenócio.
JP6351582B2 (ja) * 2012-06-19 2018-07-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ アシルＣｏＡ：リゾホスファチジルコリンアシルトランスフェラーゼおよびリン脂質：ジアシルグリセロールアシルトランスフェラーゼの同時発現による多価不飽和脂肪酸の産生の改善

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CL2014003418A1 (es) 2015-08-28
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JP2015519921A (ja) 2015-07-16
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CA2877279A1 (en) 2013-12-27
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US9347075B2 (en) 2016-05-24
EP2861727A1 (en) 2015-04-22
WO2013192002A1 (en) 2013-12-27
JP6351582B2 (ja) 2018-07-04
CL2014003419A1 (es) 2015-08-28
WO2013192007A1 (en) 2013-12-27
DK2861727T3 (da) 2018-06-14
CA2877334A1 (en) 2013-12-27
BR112014031784A2 (pt) 2019-10-01
US9416382B2 (en) 2016-08-16
EP2861728B1 (en) 2017-12-27

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