DK2777405T3 - Nye Lactobacillus-stammer og anvendelser deraf - Google Patents

Nye Lactobacillus-stammer og anvendelser deraf Download PDF

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DK2777405T3
DK2777405T3 DK13159061.4T DK13159061T DK2777405T3 DK 2777405 T3 DK2777405 T3 DK 2777405T3 DK 13159061 T DK13159061 T DK 13159061T DK 2777405 T3 DK2777405 T3 DK 2777405T3
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lactic acid
pharmaceutical
food
lactobacillus
acid bacteria
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DK13159061.4T
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Christine Lang
Andreas Heilmann
Detlef Goelling
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Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • A23L3/3571—
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/121—Brevis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/10—General cosmetic use
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/24—Lactobacillus brevis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/25—Lactobacillus plantarum

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  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
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  • Polymers & Plastics (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • Food Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
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Claims (12)

1. Mælkesyrebakterie, som indleveret under Lactobacillus brevis DSM 22721, eller et lysat deraf, til brug som et tilsætningsmiddel i fødevarer, hvor mælkesyrebakterien er heteolaktisk, og hvor mælkesyrebakteriet eller dens lysat inhiberer væksten af mindst en svampeorganisme.
2. Mælkesyrebakterie ifølge krav 1, hvor mælkesyrebakterie er kendetegnet ved, at det kan testes ifølge de følgende trin: a) etablering af et substrat, b) etablering af et præparat, som omfatter de mælkesyrebakterier, der skal undersøges, c) præparatet, der omfatter mælkesyrebakterierne, tilsættes til lysatet, eventuelt efterfulgt af et fermenteringstrin, d) produktet ifølge trin c) samples i prøveholdere, hvorved der dannes testprøver, e) et forudbestemt antal svamesporer tilsættes til mindst en testprøve, f) inkubation af den i trin e) opnåede testprøve udføres over en forudbestemt periode ved en forudbstemt temperatur, g) detektering, eventuelt efterfulgt af evaluering af svampevækst.
3. Mælkesyrebakterie ifølge krav 1 eller 2, hvor mælkesyrebakterien er en levende mælkesyrebakterie.
4. Mælkesyrebakterie eller et lysat deraf ifølge mindst et af de foregående krav, hvor svampeorganismen er valgt fra gruppen, der omfatter slægten Penicillium, fortrinsvis Penicillium commune eller Penicillium roqueforti, slægten Aspergillus og slægten Alternaria, fortrinsvis Al-ternaria alternata, Penicillium expansum, Penicillium citrinum, Penicillium digitatum, Penicillium italicum, Scopulariopsis breviacaulis, Aspergillus flavus, Aspergillus parasiticus, Botrytis cinerea, Rhizopus sp., Mucor sp., Eurotium herbariorum, Geotrichum candidum, Cladosporium herbarum, Fusarium sambucinum, Phytophora infestans og Sclerotinia scerotiorum.
5. Anvendelse af mælkesyrebakterier eller et lysat deraf ifølge mindst et af kravene 1 til 4 til fremstilling af en fødevaresammensætning, et dyrefoder eller en farmaceutisk eller kosmetisk sammensætning, hvor mælkesyrebakterierne, fortrinsvis levende mælkesyrebakterier, eller et lysat deraf, tilsættes til fødevaren, dyrefoderet eller den farmaceutiske eller kosmetiske sammensætning.
6. Fødevaresammensætning, dyrefoder eller farmaceutisk eller kosmetisk sammensætning, der omfatter mælkesyrebakterierne eller lysater deraf ifølge mindst et af kravene 1 til 4 i fødevaren, dyrefoderet eller den farmaceutiske eller kosmetiske sammensætning.
7. Fødevaresammensætning, dyrefoder eller farmaceutisk eller kosmetisk sammensætning ifølge det foregående krav, hvor fødevaresammensætningen, dyrefoderet, den farmaceutiske eller kosmetiske sammensætning indeholder 102 til 1015, fortrinsvis 106 eller 108 til 1012 *, især 108 til 1O10 mælkesyrebakterieceller eller lysater deraf pr. 100 g sammensætning.
8. Fødevaresammensætning, dyrefoder eller farmaceutisk eller kosmetisk sammensætning ifølge krav 6 eller 7, hvor mælkesyrebakterierne er til stede i en koncentration på 0.0001% til 0.01% fortrinsvis 0.001%.
9. Fødevaresammensætning ifølge mindst et af kravene 6 til 8, hvor fødevaresammensætningen er et kødprodukt eller et mejeriprodukt, fortrinsvis yoghurt, mælk, ost, fløde og/eller ostemasse.
10. Fremgangsmåde til konservering affødevarer, dyrefoder eller en farmaceutisk eller kosmetisk sammensætning, hvor mælkesyrebakterierne eller lysaterne deraf ifølge mindst et af kravene 1 til 4 tilsættes til fødervarerne, dyrefoderet eller den farmaceutiske eller kosmetiske sammensætning.
11. Fremgangsmåde ifølge det foregående krav, hvor der pr. 100 g fødevare, dyrefoder eller farmaceutisk eller kosmetisk sammensætning tilsættes 102 til 1015, fortrinsvis 106 eller 108 til 1012, især 108 til 101° mælkesyrebakterieceller eller lysater deraf.
12. Fremgangsmåde ifølge krav 10 eller 11, hvor mælkesyrebakterierne tilsættes i en koncentration på 0.0001% til 0.01% fortrinsvis 0.001%.
DK13159061.4T 2013-03-13 2013-03-13 Nye Lactobacillus-stammer og anvendelser deraf DK2777405T3 (da)

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EP13159061.4A EP2777405B1 (en) 2013-03-13 2013-03-13 Novel Lactobacillus strains and the uses thereof

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US (1) US11053474B2 (da)
EP (2) EP2777405B1 (da)
JP (2) JP6263205B2 (da)
KR (1) KR101831040B1 (da)
CN (1) CN105357979B (da)
AU (1) AU2014230777B2 (da)
BR (1) BR112015022833B1 (da)
CA (1) CA2903940C (da)
DK (1) DK2777405T3 (da)
ES (1) ES2710435T3 (da)
MX (1) MX2015012379A (da)
PL (1) PL2777405T3 (da)
RU (1) RU2681437C2 (da)
WO (1) WO2014140123A1 (da)

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