DK2726600T3 - Pattedyrcellekultur - Google Patents
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- DK2726600T3 DK2726600T3 DK12738674.6T DK12738674T DK2726600T3 DK 2726600 T3 DK2726600 T3 DK 2726600T3 DK 12738674 T DK12738674 T DK 12738674T DK 2726600 T3 DK2726600 T3 DK 2726600T3
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Claims (22)
- PAT E NT K RAV1. Fremgangsmåde til dyrkning af pattedyrceller, der eksprimerer et rekombi-nant protein, omfattende: at etablere en pattedyrcellekultur i et serumfrit dyrkningsmedium i en bioreaktor ved at pode bioreaktoren med mindst 0,5 x 106 til 3,0 x 106celler/ml_ i et serumfrit dyrkningsmedium; at dyrke pattedyrcellerne under en vækstfase og at supplere dyrkningsmediet med bolusforsyninger af et serumfrit næringsmedium, at starte perfusion på eller omkring dag 5 til på eller omkring dag 9 af cellekulturen, og at bevare pattedyrcellerne under en produktionsfase ved perfusion med et serumfrit perfusionsmedium, hvor det pakkede cellevolumen under produktionsfasen er mindre end eller lig med 35%.
- 2. Fremgangsmåde ifølge krav 1, hvor perfusionen begynder på eller omkring dag 5 til på eller omkring dag 7 af cellekulturen.
- 3. Fremgangsmåde ifølge krav 1, hvor perfusionen begynder, når cellerne har nået en produktionsfase.
- 4. Fremgangsmåde ifølge krav 1, der endvidere omfatter (a) at inducere cellevækstarrest med L-asparaginudsultning efterfulgt af perfusion med et serumfrit perfusionsmedium med en L-asparagi nkoncentration på 5 mM eller mindre eller (b) at inducere cellevækstarrest ved perfusion med et serumfrit perfusionsmedium med en L-asparagi nkoncentration på 5 mM eller mindre.
- 5. Fremgangsmåde ifølge krav 4, hvor koncentrationen af L-asparagin i det serumfri perfusionsmedium er mindre end eller lig med 5 mM, mindre end eller lig med 4,0 mM, mindre end eller lig med 3,0 mM, mindre end eller lig med 2,0 mM, mindre end eller lig med 1,0 mM eller er 0 mM.
- 6. Fremgangsmåde ifølge krav 4(a), hvor L-asparaginkoncentrationen af cellekulturmediet overvåges forud for og under L-asparaginudsultningen.
- 7. Fremgangsmåde ifølge krav 1, hvor det pakkede cellevolumen er mindre end eller lig med 30%.
- 8. Fremgangsmåde ifølge krav 1, hvor den levende celletæthed af pattedyrcellekulturen ved et pakket cellevolumen mindre end eller lig med 35% er 10x106 levende celler/ml til 80x106 levende celler/ml, hvor den levende celletæthed af pattedyrcellekulturen fortrinsvis er 20x106 levende celler/ml til 30x106 levende celler/ml.
- 9. Fremgangsmåde ifølge krav 1, hvor perfusionen omfatter kontinuerlig perfusion.
- 10. Fremgangsmåde ifølge krav 1, hvor perfusionshastigheden er konstant.
- 11. Fremgangsmåde ifølge krav 1, hvor perfusion udføres (a) ved en hastighed på mindre end eller lig med 1,0 arbejdsvoluminer per dag eller (b) ved en hastighed, der stiger under produktionsfasen fra 0,25 arbejdsvolumen per dag til 1,0 arbejdsvolumen per dag under celledyrkningen.
- 12. Fremgangsmåde ifølge krav 1, hvor perfusion udføres ved en hastighed, der når 1,0 arbejdsvolumen per dag på dag 9 til dag 11 af cellekulturen, fortrinsvis på dag 10 af cellekulturen.
- 13. Fremgangsmåde ifølge krav 1, hvor bolusforsyningerne af serumfrit medium begynder på dag 3 eller dag 4 af cellekulturen.
- 14. Fremgangsmåde ifølge krav 1, hvor pattedyrcellekulturen etableres ved podning af bioreaktoren med mindst 0,5 x 106 til 1,5 x 106 celler/mL i et serumfrit dyrkningsmedium.
- 15. Fremgangsmåde ifølge krav 1, der endvidere omfatter et temperaturskifte fra 36°C til 31 °C eller fra 36°C til 33°C.
- 16. Fremgangsmåde ifølge krav 15, hvor temperaturskiftet sker ved overgangen mellem vækstfasen og produktionsfasen eller under produktionsfasen.
- 17. Fremgangsmåde ifølge krav 1, hvor perfusionen gennemføres ved vekslende tangentiel strøm.
- 18. Fremgangsmåde ifølge krav 1, hvor bioreaktoren har en kapacitet på mindst 500L, fortrinsvis mindst 500L til 2000L eller mindst 1000L til 2000L.
- 19. Fremgangsmåde ifølge krav 1, hvor pattedyrcellerne er kinesiske hamster-ovarieceller (CHO).
- 20. Fremgangsmåde ifølge krav 1, hvor det rekombinante protein er valgt blandt gruppen bestående af et humant antistof, et humaniseret antistof, et kimært antistof, et rekombinant fusionsprotein eller et cytokin.
- 21. Fremgangsmåde ifølge krav 1, der endvidere omfatter et trin med at høste det rekombinante protein, der er produceret af cellekulturen.
- 22. Fremgangsmåde ifølge krav 1, hvor det rekombinante protein, der er produceret af cellekulturen, renses og formuleres til en farmaceutisk acceptabel formulering.
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